Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05065 and LRK53_RS05070 are separated by 93 nucleotides LRK53_RS05070 and LRK53_RS05075 are separated by 45 nucleotides LRK53_RS05075 and LRK53_RS05080 are separated by 3 nucleotides LRK53_RS05080 and LRK53_RS05085 are separated by 101 nucleotides
LRK53_RS05065: LRK53_RS05065 - NAD(P)-dependent alcohol dehydrogenase, at 1,195,153 to 1,196,166
_RS05065
LRK53_RS05070: LRK53_RS05070 - helix-turn-helix transcriptional regulator, at 1,196,260 to 1,196,703
_RS05070
LRK53_RS05075: LRK53_RS05075 - DUF3363 domain-containing protein, at 1,196,749 to 1,196,921
_RS05075
LRK53_RS05080: LRK53_RS05080 - integrase, at 1,196,925 to 1,197,059
_RS05080
LRK53_RS05085: LRK53_RS05085 - TonB-dependent receptor, at 1,197,161 to 1,199,533
_RS05085
Position (kb)
1196
1197 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1195.800 kb on + strand, within LRK53_RS05065 at 1195.800 kb on + strand, within LRK53_RS05065 at 1195.800 kb on + strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065 at 1196.191 kb on + strand at 1196.191 kb on + strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.192 kb on - strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.212 kb on + strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.213 kb on - strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.238 kb on + strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.239 kb on - strand at 1196.253 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.259 kb on + strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.260 kb on - strand at 1196.458 kb on + strand, within LRK53_RS05070 at 1196.557 kb on + strand, within LRK53_RS05070 at 1196.584 kb on + strand, within LRK53_RS05070 at 1196.584 kb on + strand, within LRK53_RS05070 at 1196.584 kb on + strand, within LRK53_RS05070 at 1196.585 kb on - strand, within LRK53_RS05070 at 1196.585 kb on - strand, within LRK53_RS05070 at 1196.585 kb on - strand, within LRK53_RS05070 at 1196.585 kb on - strand, within LRK53_RS05070 at 1196.585 kb on - strand, within LRK53_RS05070 at 1196.666 kb on + strand at 1196.745 kb on + strand at 1196.746 kb on - strand at 1196.750 kb on + strand at 1196.750 kb on + strand at 1196.750 kb on + strand at 1196.750 kb on + strand at 1196.751 kb on - strand at 1196.751 kb on - strand at 1196.751 kb on - strand at 1196.867 kb on - strand, within LRK53_RS05075 at 1196.899 kb on + strand, within LRK53_RS05075 at 1196.899 kb on + strand, within LRK53_RS05075 at 1196.899 kb on + strand, within LRK53_RS05075 at 1196.899 kb on + strand, within LRK53_RS05075 at 1196.899 kb on + strand, within LRK53_RS05075 at 1196.899 kb on + strand, within LRK53_RS05075 at 1196.899 kb on + strand, within LRK53_RS05075 at 1196.900 kb on - strand, within LRK53_RS05075 at 1196.900 kb on - strand, within LRK53_RS05075 at 1196.900 kb on - strand, within LRK53_RS05075 at 1196.900 kb on - strand, within LRK53_RS05075 at 1196.900 kb on - strand, within LRK53_RS05075 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.982 kb on + strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1196.983 kb on - strand, within LRK53_RS05080 at 1197.073 kb on + strand at 1197.073 kb on + strand at 1197.073 kb on + strand at 1197.073 kb on + strand at 1197.073 kb on + strand at 1197.073 kb on + strand at 1197.074 kb on - strand at 1197.074 kb on - strand at 1197.074 kb on - strand at 1197.074 kb on - strand at 1197.074 kb on - strand at 1197.074 kb on - strand at 1197.074 kb on - strand at 1197.074 kb on - strand at 1197.162 kb on + strand at 1197.162 kb on + strand at 1197.162 kb on + strand at 1197.163 kb on - strand at 1197.171 kb on + strand at 1197.171 kb on + strand at 1197.176 kb on + strand at 1197.176 kb on + strand at 1197.176 kb on + strand at 1197.176 kb on + strand at 1197.176 kb on + strand at 1197.177 kb on - strand at 1197.177 kb on - strand at 1197.177 kb on - strand at 1197.177 kb on - strand at 1197.177 kb on - strand at 1197.186 kb on + strand at 1197.186 kb on + strand at 1197.193 kb on - strand at 1197.193 kb on - strand at 1197.193 kb on - strand at 1197.193 kb on - strand at 1197.193 kb on - strand at 1197.193 kb on - strand at 1197.193 kb on - strand at 1197.228 kb on + strand at 1197.229 kb on - strand at 1197.330 kb on + strand at 1197.330 kb on + strand at 1197.330 kb on + strand at 1197.330 kb on + strand at 1197.330 kb on + strand at 1197.330 kb on + strand at 1197.331 kb on - strand at 1197.331 kb on - strand at 1197.415 kb on - strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.600 kb on + strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.601 kb on - strand, within LRK53_RS05085 at 1197.606 kb on + strand, within LRK53_RS05085 at 1197.607 kb on - strand, within LRK53_RS05085 at 1197.607 kb on - strand, within LRK53_RS05085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,195,800 + LRK53_RS05065 0.64 -0.7 1,195,800 + LRK53_RS05065 0.64 -0.6 1,195,800 + LRK53_RS05065 0.64 +0.4 1,195,801 - LRK53_RS05065 0.64 +0.4 1,195,801 - LRK53_RS05065 0.64 +1.0 1,195,801 - LRK53_RS05065 0.64 -0.4 1,195,801 - LRK53_RS05065 0.64 -0.9 1,196,191 + -1.2 1,196,191 + -0.6 1,196,192 - -0.6 1,196,192 - +0.2 1,196,192 - -0.4 1,196,192 - +0.1 1,196,192 - -0.0 1,196,192 - -0.0 1,196,192 - -2.0 1,196,192 - -1.4 1,196,192 - -0.0 1,196,192 - -1.0 1,196,192 - -1.0 1,196,212 + +0.2 1,196,212 + -0.0 1,196,212 + -0.8 1,196,212 + -0.8 1,196,212 + -1.6 1,196,212 + +2.0 1,196,212 + +0.5 1,196,212 + -0.9 1,196,212 + +0.2 1,196,212 + -0.0 1,196,212 + -1.0 1,196,212 + -0.6 1,196,212 + -0.9 1,196,213 - -2.2 1,196,213 - -0.6 1,196,213 - -1.4 1,196,213 - -0.6 1,196,213 - -1.0 1,196,213 - +2.0 1,196,213 - -0.0 1,196,213 - +1.0 1,196,213 - -0.7 1,196,213 - +1.0 1,196,238 + -1.0 1,196,238 + -1.4 1,196,238 + -0.8 1,196,238 + -1.0 1,196,238 + -0.0 1,196,238 + -0.0 1,196,238 + -0.4 1,196,238 + -1.0 1,196,238 + -1.4 1,196,238 + +2.0 1,196,238 + -0.4 1,196,239 - +0.2 1,196,239 - +0.4 1,196,239 - -1.5 1,196,239 - +0.6 1,196,239 - -1.0 1,196,239 - -0.6 1,196,239 - -1.4 1,196,239 - -1.4 1,196,239 - +0.4 1,196,253 + -0.6 1,196,259 + -0.4 1,196,259 + -0.0 1,196,259 + +3.3 1,196,259 + -1.5 1,196,259 + -0.6 1,196,259 + -0.0 1,196,259 + -0.7 1,196,259 + -0.4 1,196,259 + -0.0 1,196,259 + -1.0 1,196,259 + -1.8 1,196,259 + -1.1 1,196,259 + -0.4 1,196,260 - -0.3 1,196,260 - -0.8 1,196,260 - +0.0 1,196,260 - -1.1 1,196,260 - -0.4 1,196,260 - -2.8 1,196,260 - -0.7 1,196,260 - -1.0 1,196,260 - -0.6 1,196,260 - -0.0 1,196,260 - +1.0 1,196,260 - -0.4 1,196,260 - -1.8 1,196,458 + LRK53_RS05070 0.45 -0.6 1,196,557 + LRK53_RS05070 0.67 +0.2 1,196,584 + LRK53_RS05070 0.73 -1.4 1,196,584 + LRK53_RS05070 0.73 -1.4 1,196,584 + LRK53_RS05070 0.73 -0.6 1,196,585 - LRK53_RS05070 0.73 -0.7 1,196,585 - LRK53_RS05070 0.73 -0.0 1,196,585 - LRK53_RS05070 0.73 -2.6 1,196,585 - LRK53_RS05070 0.73 -1.6 1,196,585 - LRK53_RS05070 0.73 -2.2 1,196,666 + -1.0 1,196,745 + -1.0 1,196,746 - -2.0 1,196,750 + -0.6 1,196,750 + -0.8 1,196,750 + -0.0 1,196,750 + +1.0 1,196,751 - +1.0 1,196,751 - +0.4 1,196,751 - +1.0 1,196,867 - LRK53_RS05075 0.68 +1.0 1,196,899 + LRK53_RS05075 0.87 +1.0 1,196,899 + LRK53_RS05075 0.87 +1.0 1,196,899 + LRK53_RS05075 0.87 -1.6 1,196,899 + LRK53_RS05075 0.87 -1.3 1,196,899 + LRK53_RS05075 0.87 +1.5 1,196,899 + LRK53_RS05075 0.87 -0.0 1,196,899 + LRK53_RS05075 0.87 -0.7 1,196,900 - LRK53_RS05075 0.87 -0.0 1,196,900 - LRK53_RS05075 0.87 -0.6 1,196,900 - LRK53_RS05075 0.87 -0.4 1,196,900 - LRK53_RS05075 0.87 -0.5 1,196,900 - LRK53_RS05075 0.87 -0.3 1,196,982 + LRK53_RS05080 0.42 -0.4 1,196,982 + LRK53_RS05080 0.42 -0.3 1,196,982 + LRK53_RS05080 0.42 -1.2 1,196,982 + LRK53_RS05080 0.42 -0.0 1,196,982 + LRK53_RS05080 0.42 -0.6 1,196,982 + LRK53_RS05080 0.42 +1.0 1,196,982 + LRK53_RS05080 0.42 -0.0 1,196,982 + LRK53_RS05080 0.42 +1.0 1,196,982 + LRK53_RS05080 0.42 -1.0 1,196,982 + LRK53_RS05080 0.42 +0.2 1,196,982 + LRK53_RS05080 0.42 +1.0 1,196,982 + LRK53_RS05080 0.42 -0.1 1,196,982 + LRK53_RS05080 0.42 -0.0 1,196,982 + LRK53_RS05080 0.42 -1.5 1,196,982 + LRK53_RS05080 0.42 -0.4 1,196,982 + LRK53_RS05080 0.42 -1.3 1,196,982 + LRK53_RS05080 0.42 +0.2 1,196,983 - LRK53_RS05080 0.43 +0.3 1,196,983 - LRK53_RS05080 0.43 -0.9 1,196,983 - LRK53_RS05080 0.43 -0.3 1,196,983 - LRK53_RS05080 0.43 -0.4 1,196,983 - LRK53_RS05080 0.43 +1.0 1,196,983 - LRK53_RS05080 0.43 -0.3 1,196,983 - LRK53_RS05080 0.43 -2.0 1,196,983 - LRK53_RS05080 0.43 +0.3 1,196,983 - LRK53_RS05080 0.43 +1.0 1,196,983 - LRK53_RS05080 0.43 +0.6 1,196,983 - LRK53_RS05080 0.43 -1.2 1,196,983 - LRK53_RS05080 0.43 +0.4 1,196,983 - LRK53_RS05080 0.43 -0.8 1,196,983 - LRK53_RS05080 0.43 -0.8 1,196,983 - LRK53_RS05080 0.43 -1.4 1,196,983 - LRK53_RS05080 0.43 -1.0 1,196,983 - LRK53_RS05080 0.43 -0.0 1,196,983 - LRK53_RS05080 0.43 -0.3 1,196,983 - LRK53_RS05080 0.43 +0.4 1,196,983 - LRK53_RS05080 0.43 -0.8 1,196,983 - LRK53_RS05080 0.43 +2.0 1,196,983 - LRK53_RS05080 0.43 -0.2 1,197,073 + -1.5 1,197,073 + -1.1 1,197,073 + +0.1 1,197,073 + -0.9 1,197,073 + -2.0 1,197,073 + +1.4 1,197,074 - +1.3 1,197,074 - -2.4 1,197,074 - +1.0 1,197,074 - -1.8 1,197,074 - +1.0 1,197,074 - +1.0 1,197,074 - -0.4 1,197,074 - -1.0 1,197,162 + -0.0 1,197,162 + +0.4 1,197,162 + -0.0 1,197,163 - -1.4 1,197,171 + -2.5 1,197,171 + -0.0 1,197,176 + +1.0 1,197,176 + -0.4 1,197,176 + -0.4 1,197,176 + -0.6 1,197,176 + -0.2 1,197,177 - -0.1 1,197,177 - -0.4 1,197,177 - -0.7 1,197,177 - -1.0 1,197,177 - -1.1 1,197,186 + +0.4 1,197,186 + -0.6 1,197,193 - -1.6 1,197,193 - +0.4 1,197,193 - -0.2 1,197,193 - -2.0 1,197,193 - +1.0 1,197,193 - -0.6 1,197,193 - -0.4 1,197,228 + -1.9 1,197,229 - -0.8 1,197,330 + +0.3 1,197,330 + -1.2 1,197,330 + -0.5 1,197,330 + -1.6 1,197,330 + -1.1 1,197,330 + -0.5 1,197,331 - -1.8 1,197,331 - +3.0 1,197,415 - LRK53_RS05085 0.11 -0.0 1,197,600 + LRK53_RS05085 0.18 -0.0 1,197,600 + LRK53_RS05085 0.18 -1.6 1,197,600 + LRK53_RS05085 0.18 -0.9 1,197,600 + LRK53_RS05085 0.18 -1.4 1,197,600 + LRK53_RS05085 0.18 -1.0 1,197,600 + LRK53_RS05085 0.18 -0.4 1,197,600 + LRK53_RS05085 0.18 -0.0 1,197,600 + LRK53_RS05085 0.18 -0.0 1,197,600 + LRK53_RS05085 0.18 -0.8 1,197,600 + LRK53_RS05085 0.18 -1.8 1,197,600 + LRK53_RS05085 0.18 -0.6 1,197,600 + LRK53_RS05085 0.18 -0.8 1,197,600 + LRK53_RS05085 0.18 -0.0 1,197,601 - LRK53_RS05085 0.19 +0.2 1,197,601 - LRK53_RS05085 0.19 -0.6 1,197,601 - LRK53_RS05085 0.19 -0.4 1,197,601 - LRK53_RS05085 0.19 -1.4 1,197,601 - LRK53_RS05085 0.19 -2.4 1,197,601 - LRK53_RS05085 0.19 -2.2 1,197,601 - LRK53_RS05085 0.19 -0.6 1,197,601 - LRK53_RS05085 0.19 -0.9 1,197,601 - LRK53_RS05085 0.19 -1.1 1,197,601 - LRK53_RS05085 0.19 -0.0 1,197,601 - LRK53_RS05085 0.19 -1.0 1,197,601 - LRK53_RS05085 0.19 -1.3 1,197,601 - LRK53_RS05085 0.19 -0.8 1,197,601 - LRK53_RS05085 0.19 -1.2 1,197,606 + LRK53_RS05085 0.19 -0.9 1,197,607 - LRK53_RS05085 0.19 +0.3 1,197,607 - LRK53_RS05085 0.19 -1.0
Or see this region's nucleotide sequence