Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05055 and LRK53_RS05060 are separated by 75 nucleotides LRK53_RS05060 and LRK53_RS05065 are separated by 164 nucleotides
LRK53_RS05055: LRK53_RS05055 - ATP-dependent chaperone ClpB, at 1,192,034 to 1,194,631
_RS05055
LRK53_RS05060: LRK53_RS05060 - hypothetical protein, at 1,194,707 to 1,194,988
_RS05060
LRK53_RS05065: LRK53_RS05065 - NAD(P)-dependent alcohol dehydrogenase, at 1,195,153 to 1,196,166
_RS05065
Position (kb)
1194
1195 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1193.990 kb on + strand, within LRK53_RS05055 at 1193.990 kb on + strand, within LRK53_RS05055 at 1193.990 kb on + strand, within LRK53_RS05055 at 1193.990 kb on + strand, within LRK53_RS05055 at 1193.991 kb on - strand, within LRK53_RS05055 at 1194.014 kb on + strand, within LRK53_RS05055 at 1194.014 kb on + strand, within LRK53_RS05055 at 1194.015 kb on - strand, within LRK53_RS05055 at 1194.015 kb on - strand, within LRK53_RS05055 at 1194.045 kb on - strand, within LRK53_RS05055 at 1194.254 kb on + strand, within LRK53_RS05055 at 1194.254 kb on + strand, within LRK53_RS05055 at 1194.254 kb on + strand, within LRK53_RS05055 at 1194.255 kb on - strand, within LRK53_RS05055 at 1194.255 kb on - strand, within LRK53_RS05055 at 1194.255 kb on - strand, within LRK53_RS05055 at 1194.255 kb on - strand, within LRK53_RS05055 at 1194.255 kb on - strand, within LRK53_RS05055 at 1194.255 kb on - strand, within LRK53_RS05055 at 1194.395 kb on + strand at 1194.395 kb on + strand at 1194.396 kb on - strand at 1194.396 kb on - strand at 1194.396 kb on - strand at 1194.396 kb on - strand at 1194.396 kb on - strand at 1194.396 kb on - strand at 1194.396 kb on - strand at 1194.488 kb on + strand at 1194.489 kb on - strand at 1194.489 kb on - strand at 1194.810 kb on + strand, within LRK53_RS05060 at 1194.811 kb on - strand, within LRK53_RS05060 at 1194.900 kb on + strand, within LRK53_RS05060 at 1194.900 kb on + strand, within LRK53_RS05060 at 1194.900 kb on + strand, within LRK53_RS05060 at 1194.901 kb on - strand, within LRK53_RS05060 at 1194.901 kb on - strand, within LRK53_RS05060 at 1195.049 kb on + strand at 1195.049 kb on + strand at 1195.050 kb on - strand at 1195.092 kb on - strand at 1195.163 kb on + strand at 1195.163 kb on + strand at 1195.163 kb on + strand at 1195.164 kb on - strand at 1195.164 kb on - strand at 1195.225 kb on - strand at 1195.800 kb on + strand, within LRK53_RS05065 at 1195.800 kb on + strand, within LRK53_RS05065 at 1195.800 kb on + strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065 at 1195.801 kb on - strand, within LRK53_RS05065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,193,990 + LRK53_RS05055 0.75 -1.9 1,193,990 + LRK53_RS05055 0.75 -1.6 1,193,990 + LRK53_RS05055 0.75 -0.1 1,193,990 + LRK53_RS05055 0.75 -0.0 1,193,991 - LRK53_RS05055 0.75 -0.3 1,194,014 + LRK53_RS05055 0.76 -1.7 1,194,014 + LRK53_RS05055 0.76 -0.0 1,194,015 - LRK53_RS05055 0.76 -1.6 1,194,015 - LRK53_RS05055 0.76 -2.7 1,194,045 - LRK53_RS05055 0.77 -1.1 1,194,254 + LRK53_RS05055 0.85 +1.0 1,194,254 + LRK53_RS05055 0.85 -1.6 1,194,254 + LRK53_RS05055 0.85 -0.5 1,194,255 - LRK53_RS05055 0.85 -0.6 1,194,255 - LRK53_RS05055 0.85 -1.6 1,194,255 - LRK53_RS05055 0.85 -0.9 1,194,255 - LRK53_RS05055 0.85 -0.6 1,194,255 - LRK53_RS05055 0.85 -1.4 1,194,255 - LRK53_RS05055 0.85 +1.0 1,194,395 + -0.7 1,194,395 + +1.5 1,194,396 - +2.0 1,194,396 - -1.0 1,194,396 - +0.4 1,194,396 - -1.4 1,194,396 - -0.6 1,194,396 - +0.2 1,194,396 - -1.0 1,194,488 + -0.0 1,194,489 - -0.5 1,194,489 - -0.6 1,194,810 + LRK53_RS05060 0.37 -0.0 1,194,811 - LRK53_RS05060 0.37 -1.4 1,194,900 + LRK53_RS05060 0.68 -0.0 1,194,900 + LRK53_RS05060 0.68 +0.1 1,194,900 + LRK53_RS05060 0.68 -0.6 1,194,901 - LRK53_RS05060 0.69 -1.2 1,194,901 - LRK53_RS05060 0.69 -1.0 1,195,049 + -0.7 1,195,049 + +0.4 1,195,050 - -0.9 1,195,092 - +0.5 1,195,163 + +0.5 1,195,163 + -1.6 1,195,163 + -0.0 1,195,164 - +1.0 1,195,164 - -1.4 1,195,225 - -0.8 1,195,800 + LRK53_RS05065 0.64 -0.7 1,195,800 + LRK53_RS05065 0.64 -0.6 1,195,800 + LRK53_RS05065 0.64 +0.4 1,195,801 - LRK53_RS05065 0.64 +0.4 1,195,801 - LRK53_RS05065 0.64 +1.0 1,195,801 - LRK53_RS05065 0.64 -0.4 1,195,801 - LRK53_RS05065 0.64 -0.9
Or see this region's nucleotide sequence