Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05010 and LRK53_RS05015 are separated by 332 nucleotides LRK53_RS05015 and LRK53_RS05020 are separated by 3 nucleotides
LRK53_RS05010: LRK53_RS05010 - beta-phosphoglucomutase, at 1,178,764 to 1,181,787
_RS05010
LRK53_RS05015: LRK53_RS05015 - LacI family DNA-binding transcriptional regulator, at 1,182,120 to 1,183,124
_RS05015
LRK53_RS05020: LRK53_RS05020 - HAMP domain-containing histidine kinase, at 1,183,128 to 1,184,777
_RS05020
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1181.264 kb on + strand, within LRK53_RS05010 at 1181.514 kb on + strand at 1181.514 kb on + strand at 1181.514 kb on + strand at 1181.515 kb on - strand at 1181.515 kb on - strand at 1181.546 kb on + strand at 1181.547 kb on - strand at 1181.547 kb on - strand at 1181.774 kb on + strand at 1181.774 kb on + strand at 1181.774 kb on + strand at 1181.774 kb on + strand at 1181.774 kb on + strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.775 kb on - strand at 1181.883 kb on - strand at 1181.883 kb on - strand at 1182.288 kb on + strand, within LRK53_RS05015 at 1182.288 kb on + strand, within LRK53_RS05015 at 1182.288 kb on + strand, within LRK53_RS05015 at 1182.288 kb on + strand, within LRK53_RS05015 at 1182.288 kb on + strand, within LRK53_RS05015 at 1182.288 kb on + strand, within LRK53_RS05015 at 1182.288 kb on + strand, within LRK53_RS05015 at 1182.289 kb on - strand, within LRK53_RS05015 at 1182.289 kb on - strand, within LRK53_RS05015 at 1182.289 kb on - strand, within LRK53_RS05015 at 1182.289 kb on - strand, within LRK53_RS05015 at 1182.289 kb on - strand, within LRK53_RS05015 at 1182.335 kb on + strand, within LRK53_RS05015 at 1182.335 kb on + strand, within LRK53_RS05015 at 1182.346 kb on + strand, within LRK53_RS05015 at 1182.349 kb on + strand, within LRK53_RS05015 at 1182.350 kb on - strand, within LRK53_RS05015 at 1182.350 kb on - strand, within LRK53_RS05015 at 1182.350 kb on - strand, within LRK53_RS05015 at 1182.350 kb on - strand, within LRK53_RS05015 at 1182.957 kb on + strand, within LRK53_RS05015 at 1182.958 kb on - strand, within LRK53_RS05015 at 1182.958 kb on - strand, within LRK53_RS05015 at 1182.958 kb on - strand, within LRK53_RS05015 at 1182.958 kb on - strand, within LRK53_RS05015 at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.234 kb on + strand at 1183.235 kb on - strand at 1183.235 kb on - strand at 1183.235 kb on - strand at 1183.235 kb on - strand at 1183.235 kb on - strand at 1183.235 kb on - strand at 1183.235 kb on - strand at 1183.279 kb on + strand at 1183.279 kb on + strand at 1183.279 kb on + strand at 1183.280 kb on - strand at 1183.280 kb on - strand at 1183.280 kb on - strand at 1183.280 kb on - strand at 1183.280 kb on - strand at 1183.280 kb on - strand at 1183.280 kb on - strand at 1183.280 kb on - strand at 1183.300 kb on + strand, within LRK53_RS05020 at 1183.300 kb on + strand, within LRK53_RS05020 at 1183.301 kb on - strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.315 kb on + strand, within LRK53_RS05020 at 1183.316 kb on - strand, within LRK53_RS05020 at 1183.316 kb on - strand, within LRK53_RS05020 at 1183.316 kb on - strand, within LRK53_RS05020 at 1183.316 kb on - strand, within LRK53_RS05020 at 1183.316 kb on - strand, within LRK53_RS05020 at 1183.316 kb on - strand, within LRK53_RS05020 at 1183.390 kb on - strand, within LRK53_RS05020 at 1183.390 kb on - strand, within LRK53_RS05020 at 1183.585 kb on + strand, within LRK53_RS05020 at 1183.585 kb on + strand, within LRK53_RS05020 at 1183.585 kb on + strand, within LRK53_RS05020 at 1183.585 kb on + strand, within LRK53_RS05020 at 1183.585 kb on + strand, within LRK53_RS05020 at 1183.585 kb on + strand, within LRK53_RS05020 at 1183.585 kb on + strand, within LRK53_RS05020 at 1183.586 kb on - strand, within LRK53_RS05020 at 1183.586 kb on - strand, within LRK53_RS05020 at 1183.586 kb on - strand, within LRK53_RS05020 at 1183.586 kb on - strand, within LRK53_RS05020 at 1183.656 kb on + strand, within LRK53_RS05020 at 1184.011 kb on + strand, within LRK53_RS05020 at 1184.011 kb on + strand, within LRK53_RS05020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,181,264 + LRK53_RS05010 0.83 -0.6 1,181,514 + +0.2 1,181,514 + -1.3 1,181,514 + -1.4 1,181,515 - -2.0 1,181,515 - +0.3 1,181,546 + -1.0 1,181,547 - -0.4 1,181,547 - -0.3 1,181,774 + +0.4 1,181,774 + -0.5 1,181,774 + +0.1 1,181,774 + -0.6 1,181,774 + +0.6 1,181,775 - -1.0 1,181,775 - +0.4 1,181,775 - -0.8 1,181,775 - -1.4 1,181,775 - -0.0 1,181,775 - +0.5 1,181,775 - -0.2 1,181,775 - -0.3 1,181,775 - -2.5 1,181,775 - -0.6 1,181,775 - -0.0 1,181,775 - -1.2 1,181,775 - -0.5 1,181,883 - -2.1 1,181,883 - -0.4 1,182,288 + LRK53_RS05015 0.17 -1.0 1,182,288 + LRK53_RS05015 0.17 -2.4 1,182,288 + LRK53_RS05015 0.17 -1.1 1,182,288 + LRK53_RS05015 0.17 +1.0 1,182,288 + LRK53_RS05015 0.17 -0.8 1,182,288 + LRK53_RS05015 0.17 -0.6 1,182,288 + LRK53_RS05015 0.17 +1.0 1,182,289 - LRK53_RS05015 0.17 -0.4 1,182,289 - LRK53_RS05015 0.17 -0.0 1,182,289 - LRK53_RS05015 0.17 -1.8 1,182,289 - LRK53_RS05015 0.17 -1.0 1,182,289 - LRK53_RS05015 0.17 -1.0 1,182,335 + LRK53_RS05015 0.21 -1.4 1,182,335 + LRK53_RS05015 0.21 -0.0 1,182,346 + LRK53_RS05015 0.22 +0.4 1,182,349 + LRK53_RS05015 0.23 -0.0 1,182,350 - LRK53_RS05015 0.23 -1.4 1,182,350 - LRK53_RS05015 0.23 -1.2 1,182,350 - LRK53_RS05015 0.23 -1.5 1,182,350 - LRK53_RS05015 0.23 -1.0 1,182,957 + LRK53_RS05015 0.83 -0.4 1,182,958 - LRK53_RS05015 0.83 -0.0 1,182,958 - LRK53_RS05015 0.83 -0.6 1,182,958 - LRK53_RS05015 0.83 -0.0 1,182,958 - LRK53_RS05015 0.83 +0.2 1,183,234 + -0.0 1,183,234 + -1.0 1,183,234 + -0.5 1,183,234 + +1.0 1,183,234 + -1.0 1,183,234 + -2.0 1,183,234 + -1.0 1,183,234 + -1.5 1,183,234 + -1.4 1,183,234 + +0.4 1,183,234 + -0.6 1,183,234 + -0.4 1,183,234 + +0.3 1,183,235 - +1.0 1,183,235 - -0.5 1,183,235 - -2.0 1,183,235 - -1.0 1,183,235 - +1.0 1,183,235 - -0.6 1,183,235 - -2.0 1,183,279 + +0.3 1,183,279 + -0.6 1,183,279 + +3.3 1,183,280 - -0.6 1,183,280 - -0.0 1,183,280 - -0.0 1,183,280 - +1.0 1,183,280 - +2.0 1,183,280 - -0.6 1,183,280 - -0.2 1,183,280 - -0.0 1,183,300 + LRK53_RS05020 0.10 -1.9 1,183,300 + LRK53_RS05020 0.10 +0.4 1,183,301 - LRK53_RS05020 0.10 -1.0 1,183,315 + LRK53_RS05020 0.11 -0.0 1,183,315 + LRK53_RS05020 0.11 -0.0 1,183,315 + LRK53_RS05020 0.11 -1.0 1,183,315 + LRK53_RS05020 0.11 +2.0 1,183,315 + LRK53_RS05020 0.11 -0.0 1,183,315 + LRK53_RS05020 0.11 +0.4 1,183,315 + LRK53_RS05020 0.11 +1.0 1,183,315 + LRK53_RS05020 0.11 -1.2 1,183,315 + LRK53_RS05020 0.11 +2.6 1,183,315 + LRK53_RS05020 0.11 -0.4 1,183,316 - LRK53_RS05020 0.11 +2.0 1,183,316 - LRK53_RS05020 0.11 -1.8 1,183,316 - LRK53_RS05020 0.11 +2.6 1,183,316 - LRK53_RS05020 0.11 +0.4 1,183,316 - LRK53_RS05020 0.11 +1.5 1,183,316 - LRK53_RS05020 0.11 -1.4 1,183,390 - LRK53_RS05020 0.16 -1.4 1,183,390 - LRK53_RS05020 0.16 +0.6 1,183,585 + LRK53_RS05020 0.28 -0.9 1,183,585 + LRK53_RS05020 0.28 -1.0 1,183,585 + LRK53_RS05020 0.28 -1.6 1,183,585 + LRK53_RS05020 0.28 +1.0 1,183,585 + LRK53_RS05020 0.28 +1.0 1,183,585 + LRK53_RS05020 0.28 -2.0 1,183,585 + LRK53_RS05020 0.28 +0.4 1,183,586 - LRK53_RS05020 0.28 -1.6 1,183,586 - LRK53_RS05020 0.28 +0.4 1,183,586 - LRK53_RS05020 0.28 +1.0 1,183,586 - LRK53_RS05020 0.28 -1.1 1,183,656 + LRK53_RS05020 0.32 -1.8 1,184,011 + LRK53_RS05020 0.54 -0.3 1,184,011 + LRK53_RS05020 0.54 -0.6
Or see this region's nucleotide sequence