Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04500 and LRK53_RS04505 are separated by 609 nucleotides LRK53_RS04505 and LRK53_RS04510 are separated by 633 nucleotides
LRK53_RS04500: LRK53_RS04500 - glycine cleavage system protein GcvH, at 1,045,934 to 1,046,329
_RS04500
LRK53_RS04505: LRK53_RS04505 - met regulon transcriptional regulator MetJ, at 1,046,939 to 1,047,166
_RS04505
LRK53_RS04510: LRK53_RS04510 - energy transducer TonB, at 1,047,800 to 1,048,828
_RS04510
Position (kb)
1046
1047
1048 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.018 kb on - strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.058 kb on - strand, within LRK53_RS04500 at 1046.058 kb on - strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.561 kb on - strand at 1046.719 kb on + strand at 1046.720 kb on - strand at 1046.731 kb on - strand at 1046.737 kb on + strand at 1046.761 kb on - strand at 1046.761 kb on - strand at 1046.764 kb on - strand at 1046.764 kb on - strand at 1046.781 kb on + strand at 1046.781 kb on + strand at 1046.904 kb on + strand at 1046.905 kb on - strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.955 kb on - strand at 1046.955 kb on - strand at 1046.955 kb on - strand at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.997 kb on + strand, within LRK53_RS04505 at 1046.997 kb on + strand, within LRK53_RS04505 at 1047.470 kb on + strand at 1047.470 kb on + strand at 1047.470 kb on + strand at 1047.470 kb on + strand at 1047.470 kb on + strand at 1047.471 kb on - strand at 1047.471 kb on - strand at 1047.471 kb on - strand at 1047.471 kb on - strand at 1047.471 kb on - strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.495 kb on + strand at 1047.496 kb on - strand at 1047.496 kb on - strand at 1047.496 kb on - strand at 1047.496 kb on - strand at 1047.501 kb on + strand at 1047.501 kb on + strand at 1047.698 kb on + strand at 1047.698 kb on + strand at 1047.698 kb on + strand at 1047.698 kb on + strand at 1047.698 kb on + strand at 1047.699 kb on - strand at 1047.699 kb on - strand at 1047.699 kb on - strand at 1047.699 kb on - strand at 1047.774 kb on + strand at 1047.774 kb on + strand at 1047.774 kb on + strand at 1047.774 kb on + strand at 1047.774 kb on + strand at 1047.775 kb on - strand at 1047.775 kb on - strand at 1047.775 kb on - strand at 1047.785 kb on - strand at 1047.812 kb on + strand at 1047.812 kb on + strand at 1047.812 kb on + strand at 1047.812 kb on + strand at 1047.812 kb on + strand at 1047.812 kb on + strand at 1047.813 kb on - strand at 1047.813 kb on - strand at 1047.813 kb on - strand at 1047.813 kb on - strand at 1047.813 kb on - strand at 1047.813 kb on - strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.816 kb on + strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1047.817 kb on - strand at 1048.055 kb on - strand, within LRK53_RS04510 at 1048.076 kb on + strand, within LRK53_RS04510 at 1048.076 kb on + strand, within LRK53_RS04510 at 1048.077 kb on - strand, within LRK53_RS04510 at 1048.077 kb on - strand, within LRK53_RS04510 at 1048.077 kb on - strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.112 kb on + strand, within LRK53_RS04510 at 1048.113 kb on - strand, within LRK53_RS04510 at 1048.113 kb on - strand, within LRK53_RS04510 at 1048.113 kb on - strand, within LRK53_RS04510 at 1048.113 kb on - strand, within LRK53_RS04510 at 1048.113 kb on - strand, within LRK53_RS04510 at 1048.113 kb on - strand, within LRK53_RS04510 at 1048.113 kb on - strand, within LRK53_RS04510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,046,017 + LRK53_RS04500 0.21 +2.0 1,046,017 + LRK53_RS04500 0.21 -2.0 1,046,017 + LRK53_RS04500 0.21 +1.0 1,046,017 + LRK53_RS04500 0.21 +1.0 1,046,018 - LRK53_RS04500 0.21 -1.6 1,046,057 + LRK53_RS04500 0.31 -0.8 1,046,057 + LRK53_RS04500 0.31 -1.6 1,046,057 + LRK53_RS04500 0.31 -0.3 1,046,057 + LRK53_RS04500 0.31 +1.0 1,046,057 + LRK53_RS04500 0.31 -0.6 1,046,057 + LRK53_RS04500 0.31 -1.7 1,046,058 - LRK53_RS04500 0.31 -1.6 1,046,058 - LRK53_RS04500 0.31 -1.2 1,046,144 + LRK53_RS04500 0.53 -0.6 1,046,144 + LRK53_RS04500 0.53 +1.0 1,046,144 + LRK53_RS04500 0.53 +0.4 1,046,144 + LRK53_RS04500 0.53 +1.4 1,046,144 + LRK53_RS04500 0.53 +2.0 1,046,144 + LRK53_RS04500 0.53 -1.5 1,046,144 + LRK53_RS04500 0.53 -2.9 1,046,144 + LRK53_RS04500 0.53 -0.6 1,046,144 + LRK53_RS04500 0.53 -0.0 1,046,144 + LRK53_RS04500 0.53 -2.1 1,046,144 + LRK53_RS04500 0.53 +1.0 1,046,144 + LRK53_RS04500 0.53 +1.0 1,046,144 + LRK53_RS04500 0.53 -1.3 1,046,144 + LRK53_RS04500 0.53 -1.6 1,046,144 + LRK53_RS04500 0.53 +0.1 1,046,144 + LRK53_RS04500 0.53 -1.3 1,046,144 + LRK53_RS04500 0.53 -2.7 1,046,144 + LRK53_RS04500 0.53 -1.3 1,046,144 + LRK53_RS04500 0.53 -2.2 1,046,144 + LRK53_RS04500 0.53 +1.6 1,046,144 + LRK53_RS04500 0.53 -1.2 1,046,144 + LRK53_RS04500 0.53 -1.7 1,046,145 - LRK53_RS04500 0.53 +1.0 1,046,145 - LRK53_RS04500 0.53 -0.0 1,046,145 - LRK53_RS04500 0.53 -0.6 1,046,145 - LRK53_RS04500 0.53 -1.5 1,046,145 - LRK53_RS04500 0.53 -1.8 1,046,145 - LRK53_RS04500 0.53 -0.6 1,046,145 - LRK53_RS04500 0.53 -0.6 1,046,145 - LRK53_RS04500 0.53 -1.2 1,046,561 - -1.0 1,046,719 + +0.8 1,046,720 - -1.4 1,046,731 - +2.0 1,046,737 + -0.6 1,046,761 - +0.4 1,046,761 - +0.2 1,046,764 - -2.3 1,046,764 - -1.6 1,046,781 + -0.8 1,046,781 + -1.6 1,046,904 + +1.0 1,046,905 - -1.0 1,046,954 + -0.0 1,046,954 + -1.8 1,046,954 + -1.6 1,046,954 + +1.6 1,046,954 + -0.0 1,046,954 + -0.2 1,046,955 - +1.0 1,046,955 - -1.0 1,046,955 - +2.0 1,046,966 + LRK53_RS04505 0.12 -0.8 1,046,966 + LRK53_RS04505 0.12 +1.0 1,046,966 + LRK53_RS04505 0.12 -0.6 1,046,966 + LRK53_RS04505 0.12 -1.9 1,046,967 - LRK53_RS04505 0.12 -1.0 1,046,967 - LRK53_RS04505 0.12 -0.6 1,046,967 - LRK53_RS04505 0.12 -1.8 1,046,967 - LRK53_RS04505 0.12 -0.6 1,046,997 + LRK53_RS04505 0.25 -1.7 1,046,997 + LRK53_RS04505 0.25 -0.6 1,047,470 + +0.2 1,047,470 + -0.0 1,047,470 + +0.2 1,047,470 + -1.0 1,047,470 + -0.0 1,047,471 - -0.6 1,047,471 - -1.0 1,047,471 - -1.3 1,047,471 - -1.8 1,047,471 - -1.0 1,047,495 + -0.3 1,047,495 + +1.0 1,047,495 + -0.0 1,047,495 + -0.5 1,047,495 + +0.4 1,047,495 + +1.0 1,047,495 + -1.0 1,047,495 + +0.4 1,047,495 + -1.2 1,047,496 - -0.0 1,047,496 - +0.4 1,047,496 - -1.0 1,047,496 - -0.1 1,047,501 + -1.0 1,047,501 + +1.0 1,047,698 + +1.0 1,047,698 + -0.6 1,047,698 + -0.2 1,047,698 + -0.8 1,047,698 + -2.3 1,047,699 - -0.0 1,047,699 - -1.6 1,047,699 - -0.6 1,047,699 - -0.3 1,047,774 + -0.0 1,047,774 + -0.6 1,047,774 + -1.0 1,047,774 + -1.0 1,047,774 + -0.6 1,047,775 - +1.0 1,047,775 - +1.0 1,047,775 - +0.2 1,047,785 - -0.6 1,047,812 + +0.4 1,047,812 + -1.3 1,047,812 + -0.8 1,047,812 + -0.0 1,047,812 + -0.6 1,047,812 + -1.9 1,047,813 - +1.0 1,047,813 - -1.0 1,047,813 - +0.4 1,047,813 - -0.2 1,047,813 - -0.6 1,047,813 - -1.0 1,047,816 + -0.2 1,047,816 + -1.0 1,047,816 + +2.6 1,047,816 + -0.3 1,047,816 + -0.2 1,047,816 + -1.8 1,047,816 + -1.8 1,047,816 + -0.6 1,047,816 + +1.6 1,047,816 + -0.0 1,047,816 + -0.7 1,047,816 + -1.0 1,047,816 + -1.6 1,047,816 + +1.0 1,047,816 + -2.2 1,047,816 + -0.9 1,047,816 + -0.6 1,047,817 - +1.0 1,047,817 - -0.0 1,047,817 - -0.8 1,047,817 - +0.2 1,047,817 - -1.8 1,047,817 - +1.0 1,047,817 - -1.0 1,047,817 - -1.2 1,047,817 - +1.0 1,047,817 - +1.0 1,047,817 - -1.6 1,047,817 - +1.0 1,047,817 - -0.2 1,047,817 - -1.6 1,047,817 - -1.2 1,047,817 - -1.6 1,047,817 - -1.0 1,047,817 - -1.3 1,047,817 - +0.4 1,047,817 - -1.2 1,048,055 - LRK53_RS04510 0.25 -0.6 1,048,076 + LRK53_RS04510 0.27 +0.4 1,048,076 + LRK53_RS04510 0.27 -0.0 1,048,077 - LRK53_RS04510 0.27 -2.8 1,048,077 - LRK53_RS04510 0.27 +0.5 1,048,077 - LRK53_RS04510 0.27 +0.4 1,048,112 + LRK53_RS04510 0.30 -0.6 1,048,112 + LRK53_RS04510 0.30 -0.2 1,048,112 + LRK53_RS04510 0.30 -1.0 1,048,112 + LRK53_RS04510 0.30 -0.2 1,048,112 + LRK53_RS04510 0.30 -0.6 1,048,112 + LRK53_RS04510 0.30 -1.3 1,048,112 + LRK53_RS04510 0.30 -0.8 1,048,112 + LRK53_RS04510 0.30 -0.6 1,048,113 - LRK53_RS04510 0.30 -0.0 1,048,113 - LRK53_RS04510 0.30 -1.2 1,048,113 - LRK53_RS04510 0.30 -0.4 1,048,113 - LRK53_RS04510 0.30 -2.7 1,048,113 - LRK53_RS04510 0.30 +1.0 1,048,113 - LRK53_RS04510 0.30 -3.3 1,048,113 - LRK53_RS04510 0.30 -0.0
Or see this region's nucleotide sequence