Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04495 and LRK53_RS04500 are separated by 91 nucleotides LRK53_RS04500 and LRK53_RS04505 are separated by 609 nucleotides
LRK53_RS04495: LRK53_RS04495 - glycine cleavage system aminomethyltransferase GcvT, at 1,044,742 to 1,045,842
_RS04495
LRK53_RS04500: LRK53_RS04500 - glycine cleavage system protein GcvH, at 1,045,934 to 1,046,329
_RS04500
LRK53_RS04505: LRK53_RS04505 - met regulon transcriptional regulator MetJ, at 1,046,939 to 1,047,166
_RS04505
Position (kb)
1045
1046
1047 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1044.994 kb on + strand, within LRK53_RS04495 at 1044.994 kb on + strand, within LRK53_RS04495 at 1044.994 kb on + strand, within LRK53_RS04495 at 1044.994 kb on + strand, within LRK53_RS04495 at 1044.995 kb on - strand, within LRK53_RS04495 at 1044.995 kb on - strand, within LRK53_RS04495 at 1044.995 kb on - strand, within LRK53_RS04495 at 1044.995 kb on - strand, within LRK53_RS04495 at 1044.995 kb on - strand, within LRK53_RS04495 at 1045.045 kb on + strand, within LRK53_RS04495 at 1045.045 kb on + strand, within LRK53_RS04495 at 1045.045 kb on + strand, within LRK53_RS04495 at 1045.045 kb on + strand, within LRK53_RS04495 at 1045.045 kb on + strand, within LRK53_RS04495 at 1045.045 kb on + strand, within LRK53_RS04495 at 1045.046 kb on - strand, within LRK53_RS04495 at 1045.046 kb on - strand, within LRK53_RS04495 at 1045.046 kb on - strand, within LRK53_RS04495 at 1045.046 kb on - strand, within LRK53_RS04495 at 1045.066 kb on + strand, within LRK53_RS04495 at 1045.182 kb on - strand, within LRK53_RS04495 at 1045.321 kb on + strand, within LRK53_RS04495 at 1045.321 kb on + strand, within LRK53_RS04495 at 1045.324 kb on - strand, within LRK53_RS04495 at 1045.801 kb on + strand at 1045.801 kb on + strand at 1045.801 kb on + strand at 1045.802 kb on - strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.860 kb on + strand at 1045.861 kb on - strand at 1045.861 kb on - strand at 1045.861 kb on - strand at 1045.861 kb on - strand at 1045.885 kb on + strand at 1045.886 kb on - strand at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.017 kb on + strand, within LRK53_RS04500 at 1046.018 kb on - strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.057 kb on + strand, within LRK53_RS04500 at 1046.058 kb on - strand, within LRK53_RS04500 at 1046.058 kb on - strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.144 kb on + strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.145 kb on - strand, within LRK53_RS04500 at 1046.561 kb on - strand at 1046.719 kb on + strand at 1046.720 kb on - strand at 1046.731 kb on - strand at 1046.737 kb on + strand at 1046.761 kb on - strand at 1046.761 kb on - strand at 1046.764 kb on - strand at 1046.764 kb on - strand at 1046.781 kb on + strand at 1046.781 kb on + strand at 1046.904 kb on + strand at 1046.905 kb on - strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.954 kb on + strand at 1046.955 kb on - strand at 1046.955 kb on - strand at 1046.955 kb on - strand at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.966 kb on + strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.967 kb on - strand, within LRK53_RS04505 at 1046.997 kb on + strand, within LRK53_RS04505 at 1046.997 kb on + strand, within LRK53_RS04505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,044,994 + LRK53_RS04495 0.23 -1.4 1,044,994 + LRK53_RS04495 0.23 +0.4 1,044,994 + LRK53_RS04495 0.23 -0.9 1,044,994 + LRK53_RS04495 0.23 -0.6 1,044,995 - LRK53_RS04495 0.23 -1.8 1,044,995 - LRK53_RS04495 0.23 -2.2 1,044,995 - LRK53_RS04495 0.23 +1.4 1,044,995 - LRK53_RS04495 0.23 -1.0 1,044,995 - LRK53_RS04495 0.23 +1.6 1,045,045 + LRK53_RS04495 0.28 +1.0 1,045,045 + LRK53_RS04495 0.28 -2.9 1,045,045 + LRK53_RS04495 0.28 -1.8 1,045,045 + LRK53_RS04495 0.28 -0.6 1,045,045 + LRK53_RS04495 0.28 -1.0 1,045,045 + LRK53_RS04495 0.28 +1.0 1,045,046 - LRK53_RS04495 0.28 -0.8 1,045,046 - LRK53_RS04495 0.28 -1.0 1,045,046 - LRK53_RS04495 0.28 -0.8 1,045,046 - LRK53_RS04495 0.28 -1.5 1,045,066 + LRK53_RS04495 0.29 -1.7 1,045,182 - LRK53_RS04495 0.40 +0.3 1,045,321 + LRK53_RS04495 0.53 -1.6 1,045,321 + LRK53_RS04495 0.53 -0.8 1,045,324 - LRK53_RS04495 0.53 +1.0 1,045,801 + +0.4 1,045,801 + +1.0 1,045,801 + -1.3 1,045,802 - -0.0 1,045,860 + -1.6 1,045,860 + -0.3 1,045,860 + +0.2 1,045,860 + -0.9 1,045,860 + -1.0 1,045,860 + -0.6 1,045,860 + -0.1 1,045,860 + +0.1 1,045,860 + +0.1 1,045,860 + -0.3 1,045,861 - -0.6 1,045,861 - +1.0 1,045,861 - -0.6 1,045,861 - -1.1 1,045,885 + -0.6 1,045,886 - +2.6 1,046,017 + LRK53_RS04500 0.21 +2.0 1,046,017 + LRK53_RS04500 0.21 -2.0 1,046,017 + LRK53_RS04500 0.21 +1.0 1,046,017 + LRK53_RS04500 0.21 +1.0 1,046,018 - LRK53_RS04500 0.21 -1.6 1,046,057 + LRK53_RS04500 0.31 -0.8 1,046,057 + LRK53_RS04500 0.31 -1.6 1,046,057 + LRK53_RS04500 0.31 -0.3 1,046,057 + LRK53_RS04500 0.31 +1.0 1,046,057 + LRK53_RS04500 0.31 -0.6 1,046,057 + LRK53_RS04500 0.31 -1.7 1,046,058 - LRK53_RS04500 0.31 -1.6 1,046,058 - LRK53_RS04500 0.31 -1.2 1,046,144 + LRK53_RS04500 0.53 -0.6 1,046,144 + LRK53_RS04500 0.53 +1.0 1,046,144 + LRK53_RS04500 0.53 +0.4 1,046,144 + LRK53_RS04500 0.53 +1.4 1,046,144 + LRK53_RS04500 0.53 +2.0 1,046,144 + LRK53_RS04500 0.53 -1.5 1,046,144 + LRK53_RS04500 0.53 -2.9 1,046,144 + LRK53_RS04500 0.53 -0.6 1,046,144 + LRK53_RS04500 0.53 -0.0 1,046,144 + LRK53_RS04500 0.53 -2.1 1,046,144 + LRK53_RS04500 0.53 +1.0 1,046,144 + LRK53_RS04500 0.53 +1.0 1,046,144 + LRK53_RS04500 0.53 -1.3 1,046,144 + LRK53_RS04500 0.53 -1.6 1,046,144 + LRK53_RS04500 0.53 +0.1 1,046,144 + LRK53_RS04500 0.53 -1.3 1,046,144 + LRK53_RS04500 0.53 -2.7 1,046,144 + LRK53_RS04500 0.53 -1.3 1,046,144 + LRK53_RS04500 0.53 -2.2 1,046,144 + LRK53_RS04500 0.53 +1.6 1,046,144 + LRK53_RS04500 0.53 -1.2 1,046,144 + LRK53_RS04500 0.53 -1.7 1,046,145 - LRK53_RS04500 0.53 +1.0 1,046,145 - LRK53_RS04500 0.53 -0.0 1,046,145 - LRK53_RS04500 0.53 -0.6 1,046,145 - LRK53_RS04500 0.53 -1.5 1,046,145 - LRK53_RS04500 0.53 -1.8 1,046,145 - LRK53_RS04500 0.53 -0.6 1,046,145 - LRK53_RS04500 0.53 -0.6 1,046,145 - LRK53_RS04500 0.53 -1.2 1,046,561 - -1.0 1,046,719 + +0.8 1,046,720 - -1.4 1,046,731 - +2.0 1,046,737 + -0.6 1,046,761 - +0.4 1,046,761 - +0.2 1,046,764 - -2.3 1,046,764 - -1.6 1,046,781 + -0.8 1,046,781 + -1.6 1,046,904 + +1.0 1,046,905 - -1.0 1,046,954 + -0.0 1,046,954 + -1.8 1,046,954 + -1.6 1,046,954 + +1.6 1,046,954 + -0.0 1,046,954 + -0.2 1,046,955 - +1.0 1,046,955 - -1.0 1,046,955 - +2.0 1,046,966 + LRK53_RS04505 0.12 -0.8 1,046,966 + LRK53_RS04505 0.12 +1.0 1,046,966 + LRK53_RS04505 0.12 -0.6 1,046,966 + LRK53_RS04505 0.12 -1.9 1,046,967 - LRK53_RS04505 0.12 -1.0 1,046,967 - LRK53_RS04505 0.12 -0.6 1,046,967 - LRK53_RS04505 0.12 -1.8 1,046,967 - LRK53_RS04505 0.12 -0.6 1,046,997 + LRK53_RS04505 0.25 -1.7 1,046,997 + LRK53_RS04505 0.25 -0.6
Or see this region's nucleotide sequence