Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04460 and LRK53_RS04465 are separated by 124 nucleotides LRK53_RS04465 and LRK53_RS04470 overlap by 1 nucleotides LRK53_RS04470 and LRK53_RS04475 are separated by 102 nucleotides LRK53_RS04475 and LRK53_RS04480 are separated by 166 nucleotides
LRK53_RS04460: LRK53_RS04460 - glycosyltransferase, at 1,035,457 to 1,037,436
_RS04460
LRK53_RS04465: LRK53_RS04465 - HigA family addiction module antitoxin, at 1,037,561 to 1,037,854
_RS04465
LRK53_RS04470: LRK53_RS04470 - type II toxin-antitoxin system RelE/ParE family toxin, at 1,037,854 to 1,038,132
_RS04470
LRK53_RS04475: LRK53_RS04475 - electron transfer flavoprotein-ubiquinone oxidoreductase, at 1,038,235 to 1,039,845
_RS04475
LRK53_RS04480: LRK53_RS04480 - amidase, at 1,040,012 to 1,041,409
_RS04480
Position (kb) 1038
1039
1040 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.862 kb on - strand at 1037.870 kb on + strand at 1037.870 kb on + strand at 1037.870 kb on + strand at 1037.870 kb on + strand at 1037.871 kb on - strand at 1037.871 kb on - strand at 1037.871 kb on - strand at 1037.882 kb on + strand, within LRK53_RS04470 at 1037.882 kb on + strand, within LRK53_RS04470 at 1037.882 kb on + strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1037.883 kb on - strand, within LRK53_RS04470 at 1038.248 kb on + strand at 1038.248 kb on + strand at 1038.248 kb on + strand at 1038.249 kb on - strand at 1038.249 kb on - strand at 1038.249 kb on - strand at 1038.296 kb on + strand at 1038.434 kb on + strand, within LRK53_RS04475 at 1038.434 kb on + strand, within LRK53_RS04475 at 1038.435 kb on - strand, within LRK53_RS04475 at 1038.435 kb on - strand, within LRK53_RS04475 at 1038.524 kb on + strand, within LRK53_RS04475 at 1038.524 kb on + strand, within LRK53_RS04475 at 1038.524 kb on + strand, within LRK53_RS04475 at 1038.525 kb on - strand, within LRK53_RS04475 at 1038.525 kb on - strand, within LRK53_RS04475 at 1038.525 kb on - strand, within LRK53_RS04475 at 1038.525 kb on - strand, within LRK53_RS04475 at 1038.525 kb on - strand, within LRK53_RS04475 at 1038.570 kb on - strand, within LRK53_RS04475 at 1039.049 kb on + strand, within LRK53_RS04475 at 1039.065 kb on - strand, within LRK53_RS04475 at 1039.065 kb on - strand, within LRK53_RS04475 at 1039.082 kb on + strand, within LRK53_RS04475 at 1039.082 kb on + strand, within LRK53_RS04475 at 1039.083 kb on - strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.115 kb on + strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.116 kb on - strand, within LRK53_RS04475 at 1039.133 kb on + strand, within LRK53_RS04475 at 1039.134 kb on - strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.137 kb on + strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.138 kb on - strand, within LRK53_RS04475 at 1039.233 kb on - strand, within LRK53_RS04475 at 1039.352 kb on + strand, within LRK53_RS04475 at 1039.353 kb on - strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.430 kb on + strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.431 kb on - strand, within LRK53_RS04475 at 1039.820 kb on + strand at 1039.820 kb on + strand at 1039.820 kb on + strand at 1039.820 kb on + strand at 1039.820 kb on + strand at 1039.820 kb on + strand at 1039.821 kb on - strand at 1039.821 kb on - strand at 1039.821 kb on - strand at 1039.821 kb on - strand at 1039.821 kb on - strand at 1039.821 kb on - strand at 1039.821 kb on - strand at 1039.821 kb on - strand at 1039.896 kb on + strand at 1039.896 kb on + strand at 1039.988 kb on - strand at 1040.123 kb on + strand at 1040.123 kb on + strand at 1040.123 kb on + strand at 1040.123 kb on + strand at 1040.124 kb on - strand at 1040.124 kb on - strand at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.165 kb on + strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.166 kb on - strand, within LRK53_RS04480 at 1040.477 kb on + strand, within LRK53_RS04480 at 1040.585 kb on + strand, within LRK53_RS04480 at 1040.585 kb on + strand, within LRK53_RS04480 at 1040.586 kb on - strand, within LRK53_RS04480 at 1040.597 kb on + strand, within LRK53_RS04480
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,037,862 - -0.6 1,037,862 - -0.0 1,037,862 - -0.8 1,037,862 - -1.0 1,037,862 - +1.0 1,037,862 - +0.7 1,037,862 - +1.0 1,037,862 - -1.8 1,037,862 - +0.4 1,037,862 - -0.2 1,037,870 + -0.0 1,037,870 + -0.6 1,037,870 + -1.3 1,037,870 + -0.8 1,037,871 - -0.6 1,037,871 - -0.0 1,037,871 - -0.3 1,037,882 + LRK53_RS04470 0.10 -0.3 1,037,882 + LRK53_RS04470 0.10 -0.8 1,037,882 + LRK53_RS04470 0.10 -0.8 1,037,883 - LRK53_RS04470 0.10 -1.0 1,037,883 - LRK53_RS04470 0.10 -0.5 1,037,883 - LRK53_RS04470 0.10 -2.5 1,037,883 - LRK53_RS04470 0.10 -1.5 1,037,883 - LRK53_RS04470 0.10 +1.0 1,037,883 - LRK53_RS04470 0.10 -0.6 1,037,883 - LRK53_RS04470 0.10 -2.0 1,037,883 - LRK53_RS04470 0.10 +1.0 1,038,248 + -1.1 1,038,248 + -1.4 1,038,248 + -1.3 1,038,249 - +2.6 1,038,249 - -1.8 1,038,249 - -0.3 1,038,296 + -1.3 1,038,434 + LRK53_RS04475 0.12 -0.0 1,038,434 + LRK53_RS04475 0.12 -1.6 1,038,435 - LRK53_RS04475 0.12 -0.0 1,038,435 - LRK53_RS04475 0.12 -0.6 1,038,524 + LRK53_RS04475 0.18 -1.6 1,038,524 + LRK53_RS04475 0.18 -0.0 1,038,524 + LRK53_RS04475 0.18 -0.0 1,038,525 - LRK53_RS04475 0.18 -0.0 1,038,525 - LRK53_RS04475 0.18 +1.0 1,038,525 - LRK53_RS04475 0.18 -1.3 1,038,525 - LRK53_RS04475 0.18 +0.2 1,038,525 - LRK53_RS04475 0.18 -0.6 1,038,570 - LRK53_RS04475 0.21 +2.0 1,039,049 + LRK53_RS04475 0.51 -0.0 1,039,065 - LRK53_RS04475 0.52 -0.9 1,039,065 - LRK53_RS04475 0.52 +1.0 1,039,082 + LRK53_RS04475 0.53 -1.0 1,039,082 + LRK53_RS04475 0.53 +1.0 1,039,083 - LRK53_RS04475 0.53 -0.5 1,039,115 + LRK53_RS04475 0.55 -0.5 1,039,115 + LRK53_RS04475 0.55 -1.6 1,039,115 + LRK53_RS04475 0.55 +1.6 1,039,115 + LRK53_RS04475 0.55 -1.0 1,039,115 + LRK53_RS04475 0.55 +1.0 1,039,115 + LRK53_RS04475 0.55 -1.1 1,039,115 + LRK53_RS04475 0.55 -0.6 1,039,115 + LRK53_RS04475 0.55 +0.4 1,039,115 + LRK53_RS04475 0.55 +1.0 1,039,115 + LRK53_RS04475 0.55 -0.6 1,039,116 - LRK53_RS04475 0.55 -0.6 1,039,116 - LRK53_RS04475 0.55 -1.0 1,039,116 - LRK53_RS04475 0.55 +1.6 1,039,116 - LRK53_RS04475 0.55 -0.6 1,039,116 - LRK53_RS04475 0.55 +1.0 1,039,116 - LRK53_RS04475 0.55 +1.0 1,039,116 - LRK53_RS04475 0.55 -0.6 1,039,116 - LRK53_RS04475 0.55 -2.5 1,039,116 - LRK53_RS04475 0.55 -0.6 1,039,116 - LRK53_RS04475 0.55 -0.0 1,039,116 - LRK53_RS04475 0.55 +2.0 1,039,116 - LRK53_RS04475 0.55 +1.0 1,039,116 - LRK53_RS04475 0.55 -1.8 1,039,116 - LRK53_RS04475 0.55 -0.6 1,039,116 - LRK53_RS04475 0.55 +1.0 1,039,133 + LRK53_RS04475 0.56 -1.8 1,039,134 - LRK53_RS04475 0.56 -1.0 1,039,137 + LRK53_RS04475 0.56 -0.6 1,039,137 + LRK53_RS04475 0.56 -0.0 1,039,137 + LRK53_RS04475 0.56 -2.0 1,039,137 + LRK53_RS04475 0.56 -1.6 1,039,137 + LRK53_RS04475 0.56 +1.0 1,039,137 + LRK53_RS04475 0.56 +0.2 1,039,137 + LRK53_RS04475 0.56 -1.3 1,039,137 + LRK53_RS04475 0.56 -0.3 1,039,137 + LRK53_RS04475 0.56 -0.0 1,039,137 + LRK53_RS04475 0.56 -1.0 1,039,137 + LRK53_RS04475 0.56 -1.3 1,039,137 + LRK53_RS04475 0.56 +0.2 1,039,137 + LRK53_RS04475 0.56 -0.0 1,039,137 + LRK53_RS04475 0.56 +1.0 1,039,137 + LRK53_RS04475 0.56 +1.0 1,039,138 - LRK53_RS04475 0.56 -0.6 1,039,138 - LRK53_RS04475 0.56 -1.0 1,039,138 - LRK53_RS04475 0.56 +0.4 1,039,138 - LRK53_RS04475 0.56 -1.6 1,039,138 - LRK53_RS04475 0.56 -1.0 1,039,138 - LRK53_RS04475 0.56 -0.0 1,039,138 - LRK53_RS04475 0.56 -1.5 1,039,138 - LRK53_RS04475 0.56 -0.0 1,039,138 - LRK53_RS04475 0.56 -0.6 1,039,138 - LRK53_RS04475 0.56 -2.0 1,039,138 - LRK53_RS04475 0.56 -0.0 1,039,138 - LRK53_RS04475 0.56 -0.6 1,039,138 - LRK53_RS04475 0.56 -0.0 1,039,138 - LRK53_RS04475 0.56 -0.8 1,039,138 - LRK53_RS04475 0.56 -0.4 1,039,138 - LRK53_RS04475 0.56 -1.0 1,039,233 - LRK53_RS04475 0.62 +1.0 1,039,352 + LRK53_RS04475 0.69 -1.3 1,039,353 - LRK53_RS04475 0.69 -0.0 1,039,430 + LRK53_RS04475 0.74 -0.0 1,039,430 + LRK53_RS04475 0.74 -0.3 1,039,430 + LRK53_RS04475 0.74 -0.0 1,039,430 + LRK53_RS04475 0.74 +1.6 1,039,430 + LRK53_RS04475 0.74 +1.6 1,039,430 + LRK53_RS04475 0.74 +1.0 1,039,430 + LRK53_RS04475 0.74 +1.0 1,039,430 + LRK53_RS04475 0.74 -1.6 1,039,430 + LRK53_RS04475 0.74 -0.3 1,039,430 + LRK53_RS04475 0.74 -1.0 1,039,431 - LRK53_RS04475 0.74 -0.0 1,039,431 - LRK53_RS04475 0.74 -0.0 1,039,431 - LRK53_RS04475 0.74 -0.6 1,039,431 - LRK53_RS04475 0.74 -1.3 1,039,431 - LRK53_RS04475 0.74 +0.4 1,039,431 - LRK53_RS04475 0.74 -2.0 1,039,431 - LRK53_RS04475 0.74 -0.6 1,039,431 - LRK53_RS04475 0.74 -0.8 1,039,820 + -1.0 1,039,820 + -0.0 1,039,820 + -1.0 1,039,820 + -0.2 1,039,820 + -1.3 1,039,820 + -0.0 1,039,821 - -0.6 1,039,821 - -1.6 1,039,821 - -0.6 1,039,821 - -2.2 1,039,821 - +0.4 1,039,821 - -1.8 1,039,821 - +0.6 1,039,821 - -0.8 1,039,896 + +2.0 1,039,896 + -4.4 1,039,988 - -1.3 1,040,123 + -0.6 1,040,123 + -0.0 1,040,123 + -1.8 1,040,123 + -1.3 1,040,124 - -0.9 1,040,124 - -0.5 1,040,165 + LRK53_RS04480 0.11 +1.0 1,040,165 + LRK53_RS04480 0.11 +1.0 1,040,165 + LRK53_RS04480 0.11 -0.6 1,040,165 + LRK53_RS04480 0.11 +0.1 1,040,165 + LRK53_RS04480 0.11 -0.6 1,040,165 + LRK53_RS04480 0.11 +1.0 1,040,165 + LRK53_RS04480 0.11 -0.5 1,040,165 + LRK53_RS04480 0.11 -0.0 1,040,166 - LRK53_RS04480 0.11 -0.5 1,040,166 - LRK53_RS04480 0.11 -0.3 1,040,166 - LRK53_RS04480 0.11 -0.8 1,040,166 - LRK53_RS04480 0.11 -0.0 1,040,166 - LRK53_RS04480 0.11 -0.8 1,040,166 - LRK53_RS04480 0.11 -0.0 1,040,166 - LRK53_RS04480 0.11 +0.2 1,040,166 - LRK53_RS04480 0.11 -1.6 1,040,166 - LRK53_RS04480 0.11 -0.0 1,040,166 - LRK53_RS04480 0.11 -1.0 1,040,166 - LRK53_RS04480 0.11 -0.0 1,040,477 + LRK53_RS04480 0.33 -0.6 1,040,585 + LRK53_RS04480 0.41 +0.4 1,040,585 + LRK53_RS04480 0.41 -0.4 1,040,586 - LRK53_RS04480 0.41 -0.8 1,040,597 + LRK53_RS04480 0.42 -0.0
Or see this region's nucleotide sequence