Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04385 and LRK53_RS04390 are separated by 119 nucleotides LRK53_RS04390 and LRK53_RS04395 are separated by 84 nucleotides LRK53_RS04395 and LRK53_RS04400 are separated by 262 nucleotides
LRK53_RS04385: LRK53_RS04385 - class I SAM-dependent methyltransferase, at 1,016,657 to 1,018,543
_RS04385
LRK53_RS04390: LRK53_RS04390 - glycosyltransferase family 2 protein, at 1,018,663 to 1,020,996
_RS04390
LRK53_RS04395: LRK53_RS04395 - hypothetical protein, at 1,021,081 to 1,021,656
_RS04395
LRK53_RS04400: LRK53_RS04400 - hypothetical protein, at 1,021,919 to 1,024,573
_RS04400
Position (kb) 1018
1019
1020
1021 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1017.889 kb on + strand, within LRK53_RS04385 at 1017.992 kb on - strand, within LRK53_RS04385 at 1018.362 kb on + strand at 1018.362 kb on + strand at 1018.513 kb on + strand at 1018.513 kb on + strand at 1018.522 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.541 kb on + strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.542 kb on - strand at 1018.545 kb on + strand at 1018.545 kb on + strand at 1018.546 kb on - strand at 1018.546 kb on - strand at 1018.546 kb on - strand at 1018.546 kb on - strand at 1018.596 kb on + strand at 1018.596 kb on + strand at 1018.597 kb on - strand at 1018.597 kb on - strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.621 kb on + strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.622 kb on - strand at 1018.629 kb on + strand at 1018.629 kb on + strand at 1018.629 kb on + strand at 1018.629 kb on + strand at 1018.629 kb on + strand at 1018.629 kb on + strand at 1018.629 kb on + strand at 1018.629 kb on + strand at 1018.630 kb on - strand at 1018.630 kb on - strand at 1018.630 kb on - strand at 1018.630 kb on - strand at 1018.650 kb on - strand at 1018.657 kb on + strand at 1018.657 kb on + strand at 1018.657 kb on + strand at 1018.851 kb on + strand at 1018.900 kb on + strand, within LRK53_RS04390 at 1018.909 kb on + strand, within LRK53_RS04390 at 1019.108 kb on - strand, within LRK53_RS04390 at 1019.108 kb on - strand, within LRK53_RS04390 at 1019.108 kb on - strand, within LRK53_RS04390 at 1019.283 kb on - strand, within LRK53_RS04390 at 1019.408 kb on + strand, within LRK53_RS04390 at 1019.438 kb on + strand, within LRK53_RS04390 at 1019.438 kb on + strand, within LRK53_RS04390 at 1019.495 kb on - strand, within LRK53_RS04390 at 1019.641 kb on + strand, within LRK53_RS04390 at 1019.641 kb on + strand, within LRK53_RS04390 at 1019.642 kb on - strand, within LRK53_RS04390 at 1019.642 kb on - strand, within LRK53_RS04390 at 1019.849 kb on - strand, within LRK53_RS04390 at 1019.867 kb on - strand, within LRK53_RS04390 at 1019.936 kb on - strand, within LRK53_RS04390 at 1020.256 kb on - strand, within LRK53_RS04390 at 1020.259 kb on + strand, within LRK53_RS04390 at 1020.476 kb on - strand, within LRK53_RS04390 at 1020.767 kb on - strand at 1020.886 kb on + strand at 1020.887 kb on - strand at 1020.887 kb on - strand at 1020.887 kb on - strand at 1020.932 kb on + strand at 1020.933 kb on - strand at 1021.160 kb on + strand, within LRK53_RS04395 at 1021.160 kb on + strand, within LRK53_RS04395 at 1021.160 kb on + strand, within LRK53_RS04395 at 1021.160 kb on + strand, within LRK53_RS04395 at 1021.160 kb on + strand, within LRK53_RS04395 at 1021.160 kb on + strand, within LRK53_RS04395 at 1021.160 kb on + strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.161 kb on - strand, within LRK53_RS04395 at 1021.179 kb on + strand, within LRK53_RS04395 at 1021.179 kb on + strand, within LRK53_RS04395 at 1021.180 kb on - strand, within LRK53_RS04395 at 1021.180 kb on - strand, within LRK53_RS04395 at 1021.180 kb on - strand, within LRK53_RS04395 at 1021.180 kb on - strand, within LRK53_RS04395 at 1021.266 kb on + strand, within LRK53_RS04395 at 1021.266 kb on + strand, within LRK53_RS04395 at 1021.267 kb on - strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.283 kb on + strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.284 kb on - strand, within LRK53_RS04395 at 1021.494 kb on + strand, within LRK53_RS04395 at 1021.494 kb on + strand, within LRK53_RS04395 at 1021.494 kb on + strand, within LRK53_RS04395 at 1021.495 kb on - strand, within LRK53_RS04395 at 1021.495 kb on - strand, within LRK53_RS04395 at 1021.504 kb on + strand, within LRK53_RS04395 at 1021.504 kb on + strand, within LRK53_RS04395 at 1021.504 kb on + strand, within LRK53_RS04395 at 1021.504 kb on + strand, within LRK53_RS04395 at 1021.504 kb on + strand, within LRK53_RS04395 at 1021.505 kb on - strand, within LRK53_RS04395 at 1021.512 kb on + strand, within LRK53_RS04395 at 1021.558 kb on - strand, within LRK53_RS04395 at 1021.638 kb on - strand at 1021.648 kb on + strand at 1021.648 kb on + strand at 1021.648 kb on + strand at 1021.648 kb on + strand at 1021.648 kb on + strand at 1021.648 kb on + strand at 1021.648 kb on + strand at 1021.649 kb on - strand at 1021.649 kb on - strand at 1021.656 kb on + strand at 1021.656 kb on + strand at 1021.657 kb on - strand at 1021.657 kb on - strand at 1021.657 kb on - strand at 1021.672 kb on - strand at 1021.678 kb on + strand at 1021.684 kb on + strand at 1021.685 kb on - strand at 1021.685 kb on - strand at 1021.685 kb on - strand at 1021.685 kb on - strand at 1021.685 kb on - strand at 1021.695 kb on + strand at 1021.695 kb on + strand at 1021.695 kb on + strand at 1021.695 kb on + strand at 1021.695 kb on + strand at 1021.695 kb on + strand at 1021.695 kb on + strand at 1021.695 kb on + strand at 1021.696 kb on - strand at 1021.696 kb on - strand at 1021.696 kb on - strand at 1021.696 kb on - strand at 1021.696 kb on - strand at 1021.696 kb on - strand at 1021.700 kb on + strand at 1021.701 kb on - strand at 1021.701 kb on - strand at 1021.701 kb on - strand at 1021.701 kb on - strand at 1021.701 kb on - strand at 1021.706 kb on - strand at 1021.712 kb on + strand at 1021.723 kb on + strand at 1021.829 kb on + strand at 1021.835 kb on - strand at 1021.839 kb on - strand at 1021.890 kb on - strand at 1021.897 kb on + strand at 1021.897 kb on + strand at 1021.899 kb on + strand at 1021.899 kb on + strand at 1021.900 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,017,889 + LRK53_RS04385 0.65 -1.5 1,017,992 - LRK53_RS04385 0.71 -1.0 1,018,362 + +1.0 1,018,362 + -3.3 1,018,513 + -0.7 1,018,513 + -0.6 1,018,522 + +1.0 1,018,541 + -1.3 1,018,541 + -0.8 1,018,541 + +2.6 1,018,541 + -1.5 1,018,541 + -0.0 1,018,541 + -0.8 1,018,541 + -0.8 1,018,541 + -1.8 1,018,541 + +0.2 1,018,541 + -0.6 1,018,542 - -1.6 1,018,542 - +0.2 1,018,542 - +0.2 1,018,542 - +2.6 1,018,542 - -0.3 1,018,542 - -0.4 1,018,542 - -0.6 1,018,542 - -0.7 1,018,542 - -0.6 1,018,542 - -0.6 1,018,545 + +0.7 1,018,545 + +0.4 1,018,546 - -2.6 1,018,546 - +1.0 1,018,546 - -0.8 1,018,546 - -0.7 1,018,596 + -0.5 1,018,596 + +0.5 1,018,597 - -0.9 1,018,597 - -0.6 1,018,621 + +2.0 1,018,621 + -0.7 1,018,621 + +1.0 1,018,621 + -0.6 1,018,621 + -0.8 1,018,621 + -1.7 1,018,621 + -0.9 1,018,621 + -0.0 1,018,621 + -0.6 1,018,621 + +1.0 1,018,621 + -1.0 1,018,621 + -0.6 1,018,621 + +0.2 1,018,621 + +0.4 1,018,621 + -2.5 1,018,622 - +0.6 1,018,622 - +0.4 1,018,622 - +1.0 1,018,622 - -0.0 1,018,622 - -0.0 1,018,622 - -0.6 1,018,622 - +2.0 1,018,622 - -0.5 1,018,622 - -0.0 1,018,622 - -1.0 1,018,622 - -1.6 1,018,622 - -0.2 1,018,629 + -0.6 1,018,629 + -1.0 1,018,629 + +1.0 1,018,629 + +0.1 1,018,629 + -1.0 1,018,629 + -1.6 1,018,629 + -0.4 1,018,629 + -0.8 1,018,630 - +0.5 1,018,630 - -0.6 1,018,630 - +0.6 1,018,630 - -0.6 1,018,650 - -0.9 1,018,657 + -1.3 1,018,657 + +0.4 1,018,657 + -1.2 1,018,851 + -0.0 1,018,900 + LRK53_RS04390 0.10 -1.8 1,018,909 + LRK53_RS04390 0.11 -0.8 1,019,108 - LRK53_RS04390 0.19 -0.0 1,019,108 - LRK53_RS04390 0.19 +1.0 1,019,108 - LRK53_RS04390 0.19 -0.0 1,019,283 - LRK53_RS04390 0.27 -1.0 1,019,408 + LRK53_RS04390 0.32 -0.6 1,019,438 + LRK53_RS04390 0.33 -0.0 1,019,438 + LRK53_RS04390 0.33 -1.0 1,019,495 - LRK53_RS04390 0.36 -0.8 1,019,641 + LRK53_RS04390 0.42 -1.0 1,019,641 + LRK53_RS04390 0.42 -1.6 1,019,642 - LRK53_RS04390 0.42 +1.0 1,019,642 - LRK53_RS04390 0.42 -1.0 1,019,849 - LRK53_RS04390 0.51 -1.0 1,019,867 - LRK53_RS04390 0.52 -0.0 1,019,936 - LRK53_RS04390 0.55 +2.0 1,020,256 - LRK53_RS04390 0.68 -1.3 1,020,259 + LRK53_RS04390 0.68 +0.4 1,020,476 - LRK53_RS04390 0.78 -0.0 1,020,767 - -0.0 1,020,886 + +0.4 1,020,887 - -1.3 1,020,887 - -0.0 1,020,887 - -1.0 1,020,932 + +0.2 1,020,933 - -1.0 1,021,160 + LRK53_RS04395 0.14 -1.3 1,021,160 + LRK53_RS04395 0.14 -0.7 1,021,160 + LRK53_RS04395 0.14 -1.3 1,021,160 + LRK53_RS04395 0.14 -0.7 1,021,160 + LRK53_RS04395 0.14 -1.0 1,021,160 + LRK53_RS04395 0.14 +0.4 1,021,160 + LRK53_RS04395 0.14 -0.2 1,021,161 - LRK53_RS04395 0.14 -1.1 1,021,161 - LRK53_RS04395 0.14 -0.0 1,021,161 - LRK53_RS04395 0.14 -1.0 1,021,161 - LRK53_RS04395 0.14 -0.3 1,021,161 - LRK53_RS04395 0.14 -1.0 1,021,161 - LRK53_RS04395 0.14 -0.0 1,021,161 - LRK53_RS04395 0.14 -0.5 1,021,161 - LRK53_RS04395 0.14 -0.0 1,021,179 + LRK53_RS04395 0.17 -0.6 1,021,179 + LRK53_RS04395 0.17 -2.3 1,021,180 - LRK53_RS04395 0.17 +1.0 1,021,180 - LRK53_RS04395 0.17 +0.7 1,021,180 - LRK53_RS04395 0.17 +0.4 1,021,180 - LRK53_RS04395 0.17 +0.8 1,021,266 + LRK53_RS04395 0.32 -1.1 1,021,266 + LRK53_RS04395 0.32 -0.0 1,021,267 - LRK53_RS04395 0.32 -1.0 1,021,283 + LRK53_RS04395 0.35 -0.9 1,021,283 + LRK53_RS04395 0.35 +0.8 1,021,283 + LRK53_RS04395 0.35 +1.0 1,021,283 + LRK53_RS04395 0.35 +1.3 1,021,283 + LRK53_RS04395 0.35 -0.6 1,021,283 + LRK53_RS04395 0.35 -1.8 1,021,283 + LRK53_RS04395 0.35 -0.6 1,021,283 + LRK53_RS04395 0.35 -0.1 1,021,283 + LRK53_RS04395 0.35 -0.6 1,021,283 + LRK53_RS04395 0.35 -1.6 1,021,283 + LRK53_RS04395 0.35 -1.3 1,021,283 + LRK53_RS04395 0.35 -0.5 1,021,283 + LRK53_RS04395 0.35 -1.0 1,021,284 - LRK53_RS04395 0.35 +2.0 1,021,284 - LRK53_RS04395 0.35 +2.0 1,021,284 - LRK53_RS04395 0.35 -0.2 1,021,284 - LRK53_RS04395 0.35 +0.6 1,021,284 - LRK53_RS04395 0.35 -1.1 1,021,284 - LRK53_RS04395 0.35 -0.2 1,021,284 - LRK53_RS04395 0.35 +0.6 1,021,284 - LRK53_RS04395 0.35 +0.2 1,021,494 + LRK53_RS04395 0.72 +0.4 1,021,494 + LRK53_RS04395 0.72 -0.0 1,021,494 + LRK53_RS04395 0.72 -0.0 1,021,495 - LRK53_RS04395 0.72 -1.6 1,021,495 - LRK53_RS04395 0.72 -0.3 1,021,504 + LRK53_RS04395 0.73 -1.6 1,021,504 + LRK53_RS04395 0.73 -1.7 1,021,504 + LRK53_RS04395 0.73 +1.0 1,021,504 + LRK53_RS04395 0.73 -1.2 1,021,504 + LRK53_RS04395 0.73 +0.2 1,021,505 - LRK53_RS04395 0.74 +2.0 1,021,512 + LRK53_RS04395 0.75 -1.3 1,021,558 - LRK53_RS04395 0.83 -0.8 1,021,638 - -0.3 1,021,648 + +1.0 1,021,648 + -0.2 1,021,648 + -0.6 1,021,648 + +0.7 1,021,648 + -0.8 1,021,648 + -0.0 1,021,648 + +2.0 1,021,649 - +0.9 1,021,649 - -1.2 1,021,656 + -2.2 1,021,656 + -0.2 1,021,657 - -0.3 1,021,657 - -0.6 1,021,657 - +0.4 1,021,672 - -0.6 1,021,678 + -0.3 1,021,684 + -0.6 1,021,685 - -0.8 1,021,685 - -1.0 1,021,685 - +2.0 1,021,685 - -0.8 1,021,685 - -0.6 1,021,695 + +1.0 1,021,695 + -1.1 1,021,695 + -0.5 1,021,695 + -1.3 1,021,695 + -0.6 1,021,695 + -0.3 1,021,695 + -1.5 1,021,695 + -0.0 1,021,696 - -1.2 1,021,696 - -1.7 1,021,696 - +0.7 1,021,696 - -0.6 1,021,696 - -0.0 1,021,696 - -0.7 1,021,700 + -0.0 1,021,701 - +1.0 1,021,701 - -0.6 1,021,701 - +1.6 1,021,701 - -0.7 1,021,701 - -2.3 1,021,706 - -1.0 1,021,712 + -0.6 1,021,723 + +0.1 1,021,829 + +1.0 1,021,835 - -0.6 1,021,839 - -0.3 1,021,890 - +0.8 1,021,897 + -0.0 1,021,897 + -0.0 1,021,899 + -1.3 1,021,899 + -0.9 1,021,900 - -1.5
Or see this region's nucleotide sequence