Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04365 and LRK53_RS04370 are separated by 272 nucleotides LRK53_RS04370 and LRK53_RS04375 are separated by 95 nucleotides LRK53_RS04375 and LRK53_RS04380 overlap by 11 nucleotides
LRK53_RS04365: LRK53_RS04365 - dTDP-glucose 4,6-dehydratase, at 1,011,965 to 1,013,035
_RS04365
LRK53_RS04370: LRK53_RS04370 - cystathionine gamma-synthase, at 1,013,308 to 1,014,474
_RS04370
LRK53_RS04375: LRK53_RS04375 - ABC transporter permease, at 1,014,570 to 1,015,328
_RS04375
LRK53_RS04380: LRK53_RS04380 - ABC transporter ATP-binding protein, at 1,015,318 to 1,016,667
_RS04380
Position (kb) 1013
1014
1015 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1012.489 kb on - strand, within LRK53_RS04365 at 1012.908 kb on + strand, within LRK53_RS04365 at 1013.038 kb on - strand at 1013.038 kb on - strand at 1013.038 kb on - strand at 1013.038 kb on - strand at 1013.038 kb on - strand at 1013.038 kb on - strand at 1013.038 kb on - strand at 1013.038 kb on - strand at 1013.040 kb on + strand at 1013.040 kb on + strand at 1013.040 kb on + strand at 1013.041 kb on - strand at 1013.041 kb on - strand at 1013.041 kb on - strand at 1013.246 kb on + strand at 1013.247 kb on - strand at 1013.247 kb on - strand at 1013.247 kb on - strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.342 kb on + strand at 1013.343 kb on - strand at 1013.343 kb on - strand at 1013.343 kb on - strand at 1013.343 kb on - strand at 1013.343 kb on - strand at 1013.343 kb on - strand at 1013.397 kb on + strand at 1013.397 kb on + strand at 1013.397 kb on + strand at 1013.397 kb on + strand at 1013.397 kb on + strand at 1013.397 kb on + strand at 1013.397 kb on + strand at 1013.397 kb on + strand at 1013.398 kb on - strand at 1013.410 kb on + strand at 1013.410 kb on + strand at 1013.410 kb on + strand at 1013.410 kb on + strand at 1013.410 kb on + strand at 1013.411 kb on - strand at 1013.411 kb on - strand at 1013.411 kb on - strand at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.425 kb on + strand, within LRK53_RS04370 at 1013.426 kb on - strand, within LRK53_RS04370 at 1013.426 kb on - strand, within LRK53_RS04370 at 1013.426 kb on - strand, within LRK53_RS04370 at 1013.426 kb on - strand, within LRK53_RS04370 at 1013.426 kb on - strand, within LRK53_RS04370 at 1013.458 kb on + strand, within LRK53_RS04370 at 1013.458 kb on + strand, within LRK53_RS04370 at 1013.458 kb on + strand, within LRK53_RS04370 at 1013.459 kb on - strand, within LRK53_RS04370 at 1013.459 kb on - strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.464 kb on + strand, within LRK53_RS04370 at 1013.465 kb on - strand, within LRK53_RS04370 at 1013.465 kb on - strand, within LRK53_RS04370 at 1013.465 kb on - strand, within LRK53_RS04370 at 1013.465 kb on - strand, within LRK53_RS04370 at 1013.484 kb on + strand, within LRK53_RS04370 at 1013.485 kb on - strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.497 kb on + strand, within LRK53_RS04370 at 1013.498 kb on - strand, within LRK53_RS04370 at 1013.626 kb on + strand, within LRK53_RS04370 at 1013.626 kb on + strand, within LRK53_RS04370 at 1013.627 kb on - strand, within LRK53_RS04370 at 1013.638 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.923 kb on + strand, within LRK53_RS04370 at 1013.924 kb on - strand, within LRK53_RS04370 at 1013.924 kb on - strand, within LRK53_RS04370 at 1013.924 kb on - strand, within LRK53_RS04370 at 1013.924 kb on - strand, within LRK53_RS04370 at 1013.924 kb on - strand, within LRK53_RS04370 at 1013.924 kb on - strand, within LRK53_RS04370 at 1013.924 kb on - strand, within LRK53_RS04370 at 1013.970 kb on + strand, within LRK53_RS04370 at 1013.970 kb on + strand, within LRK53_RS04370 at 1013.971 kb on - strand, within LRK53_RS04370 at 1014.157 kb on + strand, within LRK53_RS04370 at 1014.157 kb on + strand, within LRK53_RS04370 at 1014.157 kb on + strand, within LRK53_RS04370 at 1014.157 kb on + strand, within LRK53_RS04370 at 1014.157 kb on + strand, within LRK53_RS04370 at 1014.158 kb on - strand, within LRK53_RS04370 at 1014.598 kb on + strand at 1015.372 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,012,489 - LRK53_RS04365 0.49 +1.0 1,012,908 + LRK53_RS04365 0.88 -1.8 1,013,038 - +1.0 1,013,038 - +1.0 1,013,038 - +2.0 1,013,038 - -1.8 1,013,038 - +1.0 1,013,038 - -0.5 1,013,038 - -1.3 1,013,038 - +1.0 1,013,040 + +0.4 1,013,040 + -0.6 1,013,040 + -0.0 1,013,041 - +1.0 1,013,041 - -0.8 1,013,041 - -0.6 1,013,246 + -0.6 1,013,247 - -0.1 1,013,247 - -1.1 1,013,247 - -0.3 1,013,342 + -0.0 1,013,342 + -0.6 1,013,342 + +1.0 1,013,342 + -1.0 1,013,342 + -1.3 1,013,342 + -1.0 1,013,342 + -0.0 1,013,342 + -2.5 1,013,342 + -0.6 1,013,342 + -1.9 1,013,342 + -0.0 1,013,342 + -1.6 1,013,342 + -1.0 1,013,342 + -0.9 1,013,342 + -0.0 1,013,342 + -0.0 1,013,342 + -1.0 1,013,342 + +1.6 1,013,342 + -0.0 1,013,342 + -1.0 1,013,342 + -1.7 1,013,342 + +1.0 1,013,342 + -1.1 1,013,342 + -3.0 1,013,343 - -1.3 1,013,343 - -0.3 1,013,343 - -0.6 1,013,343 - -0.0 1,013,343 - +0.6 1,013,343 - -0.6 1,013,397 + +2.0 1,013,397 + -1.6 1,013,397 + -1.6 1,013,397 + -2.0 1,013,397 + -0.8 1,013,397 + +1.0 1,013,397 + -0.3 1,013,397 + -1.7 1,013,398 - +1.0 1,013,410 + +0.4 1,013,410 + -2.2 1,013,410 + -0.6 1,013,410 + -1.3 1,013,410 + +1.0 1,013,411 - -1.3 1,013,411 - -0.0 1,013,411 - +1.0 1,013,425 + LRK53_RS04370 0.10 -1.6 1,013,425 + LRK53_RS04370 0.10 -1.8 1,013,425 + LRK53_RS04370 0.10 -0.3 1,013,425 + LRK53_RS04370 0.10 -1.0 1,013,425 + LRK53_RS04370 0.10 -0.6 1,013,425 + LRK53_RS04370 0.10 -0.4 1,013,425 + LRK53_RS04370 0.10 -1.8 1,013,425 + LRK53_RS04370 0.10 -1.8 1,013,425 + LRK53_RS04370 0.10 -1.0 1,013,425 + LRK53_RS04370 0.10 -1.8 1,013,425 + LRK53_RS04370 0.10 -0.0 1,013,425 + LRK53_RS04370 0.10 -0.6 1,013,425 + LRK53_RS04370 0.10 -1.1 1,013,426 - LRK53_RS04370 0.10 +1.0 1,013,426 - LRK53_RS04370 0.10 -0.6 1,013,426 - LRK53_RS04370 0.10 -1.0 1,013,426 - LRK53_RS04370 0.10 +1.0 1,013,426 - LRK53_RS04370 0.10 -0.0 1,013,458 + LRK53_RS04370 0.13 -0.6 1,013,458 + LRK53_RS04370 0.13 -1.0 1,013,458 + LRK53_RS04370 0.13 -0.3 1,013,459 - LRK53_RS04370 0.13 -1.2 1,013,459 - LRK53_RS04370 0.13 -1.8 1,013,464 + LRK53_RS04370 0.13 -1.6 1,013,464 + LRK53_RS04370 0.13 -1.6 1,013,464 + LRK53_RS04370 0.13 -4.4 1,013,464 + LRK53_RS04370 0.13 -0.0 1,013,464 + LRK53_RS04370 0.13 -1.8 1,013,464 + LRK53_RS04370 0.13 -2.3 1,013,464 + LRK53_RS04370 0.13 -1.0 1,013,464 + LRK53_RS04370 0.13 -1.0 1,013,465 - LRK53_RS04370 0.13 -1.3 1,013,465 - LRK53_RS04370 0.13 +1.0 1,013,465 - LRK53_RS04370 0.13 -0.6 1,013,465 - LRK53_RS04370 0.13 -0.0 1,013,484 + LRK53_RS04370 0.15 -2.2 1,013,485 - LRK53_RS04370 0.15 -0.6 1,013,497 + LRK53_RS04370 0.16 -1.3 1,013,497 + LRK53_RS04370 0.16 -1.3 1,013,497 + LRK53_RS04370 0.16 -1.1 1,013,497 + LRK53_RS04370 0.16 -2.2 1,013,497 + LRK53_RS04370 0.16 -0.6 1,013,497 + LRK53_RS04370 0.16 -0.3 1,013,497 + LRK53_RS04370 0.16 +1.0 1,013,497 + LRK53_RS04370 0.16 -1.0 1,013,498 - LRK53_RS04370 0.16 +0.2 1,013,626 + LRK53_RS04370 0.27 -1.6 1,013,626 + LRK53_RS04370 0.27 -0.0 1,013,627 - LRK53_RS04370 0.27 -0.6 1,013,638 + LRK53_RS04370 0.28 -0.6 1,013,923 + LRK53_RS04370 0.53 -0.6 1,013,923 + LRK53_RS04370 0.53 -1.8 1,013,923 + LRK53_RS04370 0.53 -2.0 1,013,923 + LRK53_RS04370 0.53 -0.0 1,013,923 + LRK53_RS04370 0.53 -1.0 1,013,923 + LRK53_RS04370 0.53 -2.0 1,013,923 + LRK53_RS04370 0.53 +0.4 1,013,923 + LRK53_RS04370 0.53 -1.7 1,013,923 + LRK53_RS04370 0.53 +1.0 1,013,923 + LRK53_RS04370 0.53 -0.6 1,013,923 + LRK53_RS04370 0.53 -0.9 1,013,923 + LRK53_RS04370 0.53 -1.0 1,013,923 + LRK53_RS04370 0.53 -1.3 1,013,923 + LRK53_RS04370 0.53 +2.0 1,013,923 + LRK53_RS04370 0.53 -0.6 1,013,923 + LRK53_RS04370 0.53 -0.6 1,013,924 - LRK53_RS04370 0.53 -1.6 1,013,924 - LRK53_RS04370 0.53 -0.0 1,013,924 - LRK53_RS04370 0.53 -0.6 1,013,924 - LRK53_RS04370 0.53 -1.0 1,013,924 - LRK53_RS04370 0.53 -0.6 1,013,924 - LRK53_RS04370 0.53 +1.0 1,013,924 - LRK53_RS04370 0.53 -0.0 1,013,970 + LRK53_RS04370 0.57 -0.0 1,013,970 + LRK53_RS04370 0.57 -0.0 1,013,971 - LRK53_RS04370 0.57 -0.6 1,014,157 + LRK53_RS04370 0.73 -1.6 1,014,157 + LRK53_RS04370 0.73 -0.3 1,014,157 + LRK53_RS04370 0.73 -1.6 1,014,157 + LRK53_RS04370 0.73 -0.2 1,014,157 + LRK53_RS04370 0.73 -0.4 1,014,158 - LRK53_RS04370 0.73 -0.0 1,014,598 + -0.6 1,015,372 + -0.0
Or see this region's nucleotide sequence