Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04265 and LRK53_RS04270 are separated by 127 nucleotides LRK53_RS04270 and LRK53_RS04275 overlap by 4 nucleotides LRK53_RS04275 and LRK53_RS04280 are separated by 133 nucleotides LRK53_RS04280 and LRK53_RS04285 overlap by 1 nucleotides
LRK53_RS04265: LRK53_RS04265 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 993,623 to 994,519
_RS04265
LRK53_RS04270: LRK53_RS04270 - c-type cytochrome, at 994,647 to 994,955
_RS04270
LRK53_RS04275: LRK53_RS04275 - co-chaperone DjlA, at 994,952 to 995,758
_RS04275
LRK53_RS04280: LRK53_RS04280 - HAD hydrolase-like protein, at 995,892 to 996,530
_RS04280
LRK53_RS04285: LRK53_RS04285 - phosphoglycerate kinase, at 996,530 to 997,714
_RS04285
Position (kb) 994
995
996 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 994.143 kb on + strand, within LRK53_RS04265 at 994.143 kb on + strand, within LRK53_RS04265 at 994.144 kb on - strand, within LRK53_RS04265 at 994.171 kb on - strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.446 kb on + strand at 994.447 kb on - strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.556 kb on + strand at 994.612 kb on + strand at 994.657 kb on + strand at 994.657 kb on + strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.812 kb on + strand, within LRK53_RS04270 at 994.812 kb on + strand, within LRK53_RS04270 at 994.812 kb on + strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.924 kb on - strand, within LRK53_RS04270 at 994.924 kb on - strand, within LRK53_RS04270 at 994.924 kb on - strand, within LRK53_RS04270 at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 995.061 kb on - strand, within LRK53_RS04275 at 995.425 kb on + strand, within LRK53_RS04275 at 995.468 kb on + strand, within LRK53_RS04275 at 995.510 kb on + strand, within LRK53_RS04275 at 995.510 kb on + strand, within LRK53_RS04275 at 995.511 kb on - strand, within LRK53_RS04275 at 995.529 kb on - strand, within LRK53_RS04275 at 995.567 kb on + strand, within LRK53_RS04275 at 995.568 kb on - strand, within LRK53_RS04275 at 995.723 kb on + strand at 995.723 kb on + strand at 995.724 kb on - strand at 995.757 kb on - strand at 995.757 kb on - strand at 995.757 kb on - strand at 996.145 kb on + strand, within LRK53_RS04280 at 996.145 kb on + strand, within LRK53_RS04280 at 996.145 kb on + strand, within LRK53_RS04280 at 996.281 kb on - strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.325 kb on + strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.326 kb on - strand, within LRK53_RS04280 at 996.328 kb on + strand, within LRK53_RS04280 at 996.328 kb on + strand, within LRK53_RS04280 at 996.328 kb on + strand, within LRK53_RS04280 at 996.328 kb on + strand, within LRK53_RS04280 at 996.328 kb on + strand, within LRK53_RS04280 at 996.329 kb on - strand, within LRK53_RS04280 at 996.329 kb on - strand, within LRK53_RS04280 at 996.329 kb on - strand, within LRK53_RS04280 at 996.329 kb on - strand, within LRK53_RS04280 at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.531 kb on + strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.532 kb on - strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.621 kb on + strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.622 kb on - strand at 996.645 kb on + strand at 996.645 kb on + strand at 996.645 kb on + strand at 996.645 kb on + strand at 996.645 kb on + strand at 996.645 kb on + strand at 996.645 kb on + strand at 996.646 kb on - strand at 996.646 kb on - strand at 996.646 kb on - strand at 996.646 kb on - strand at 996.646 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 994,143 + LRK53_RS04265 0.58 +1.0 994,143 + LRK53_RS04265 0.58 -0.0 994,144 - LRK53_RS04265 0.58 +1.0 994,171 - LRK53_RS04265 0.61 -1.1 994,236 + LRK53_RS04265 0.68 -1.3 994,236 + LRK53_RS04265 0.68 -1.8 994,236 + LRK53_RS04265 0.68 -1.0 994,236 + LRK53_RS04265 0.68 +0.7 994,236 + LRK53_RS04265 0.68 -0.6 994,236 + LRK53_RS04265 0.68 -1.3 994,236 + LRK53_RS04265 0.68 +1.0 994,236 + LRK53_RS04265 0.68 -1.6 994,237 - LRK53_RS04265 0.68 -0.3 994,237 - LRK53_RS04265 0.68 -1.0 994,237 - LRK53_RS04265 0.68 -1.3 994,237 - LRK53_RS04265 0.68 +0.4 994,237 - LRK53_RS04265 0.68 +1.0 994,237 - LRK53_RS04265 0.68 -2.3 994,446 + -0.0 994,447 - +0.6 994,448 + +0.4 994,448 + -0.3 994,448 + +0.1 994,448 + -0.8 994,448 + -0.4 994,448 + -0.4 994,448 + -1.0 994,448 + +0.7 994,449 - -1.8 994,449 - +0.4 994,449 - +0.4 994,449 - -0.0 994,449 - -0.0 994,449 - -0.8 994,449 - -0.5 994,449 - -1.6 994,449 - -1.0 994,449 - +0.6 994,449 - +1.4 994,556 + -0.3 994,612 + -0.0 994,657 + -0.0 994,657 + -1.0 994,658 - -0.6 994,658 - -0.3 994,658 - -0.0 994,658 - -0.6 994,658 - -0.6 994,812 + LRK53_RS04270 0.53 -0.3 994,812 + LRK53_RS04270 0.53 -0.8 994,812 + LRK53_RS04270 0.53 -1.3 994,813 - LRK53_RS04270 0.54 -1.1 994,813 - LRK53_RS04270 0.54 +0.6 994,813 - LRK53_RS04270 0.54 -0.0 994,813 - LRK53_RS04270 0.54 +1.0 994,813 - LRK53_RS04270 0.54 -0.6 994,923 + LRK53_RS04270 0.89 -0.0 994,923 + LRK53_RS04270 0.89 +0.3 994,923 + LRK53_RS04270 0.89 -0.0 994,923 + LRK53_RS04270 0.89 -0.6 994,924 - LRK53_RS04270 0.90 +0.6 994,924 - LRK53_RS04270 0.90 -0.0 994,924 - LRK53_RS04270 0.90 -1.4 994,958 + -1.4 994,958 + -0.4 994,958 + -0.0 994,958 + -0.0 994,958 + +0.2 994,958 + -0.8 994,958 + -1.0 994,958 + -0.0 994,958 + +0.3 994,958 + -0.7 994,958 + -0.0 994,958 + +0.3 994,958 + -0.0 994,958 + -2.9 994,959 - +0.4 994,959 - -2.5 994,959 - +1.6 994,959 - -0.3 994,959 - -1.9 994,959 - +1.0 994,959 - -0.3 994,959 - -0.3 994,959 - -1.0 994,959 - -0.8 994,959 - -1.6 994,959 - -0.7 994,959 - -1.6 994,964 + -2.2 994,964 + -0.0 994,964 + -0.2 994,964 + -2.0 994,964 + -0.6 994,964 + -2.7 994,964 + -0.6 994,964 + -0.9 994,964 + +1.0 994,964 + -0.0 994,964 + -1.4 994,964 + +0.4 994,964 + -0.4 994,964 + -1.0 994,964 + -0.0 994,964 + -0.8 994,964 + -0.6 994,964 + +0.4 994,965 - +0.5 994,965 - -1.2 994,965 - -0.5 994,965 - -1.5 994,965 - -1.6 994,965 - -0.0 994,965 - -0.5 994,965 - -1.5 994,965 - -2.0 994,965 - -0.4 994,965 - -1.0 994,965 - -1.0 994,965 - -1.6 994,965 - -0.0 994,965 - -1.8 994,965 - -1.6 995,061 - LRK53_RS04275 0.14 -0.9 995,425 + LRK53_RS04275 0.59 -0.6 995,468 + LRK53_RS04275 0.64 -2.2 995,510 + LRK53_RS04275 0.69 +1.0 995,510 + LRK53_RS04275 0.69 -1.6 995,511 - LRK53_RS04275 0.69 -0.0 995,529 - LRK53_RS04275 0.71 -0.8 995,567 + LRK53_RS04275 0.76 -2.4 995,568 - LRK53_RS04275 0.76 -1.3 995,723 + -1.6 995,723 + -0.0 995,724 - -2.0 995,757 - +0.4 995,757 - -0.4 995,757 - -0.2 996,145 + LRK53_RS04280 0.40 -1.0 996,145 + LRK53_RS04280 0.40 -0.3 996,145 + LRK53_RS04280 0.40 +0.2 996,281 - LRK53_RS04280 0.61 -0.0 996,325 + LRK53_RS04280 0.68 -0.2 996,325 + LRK53_RS04280 0.68 -2.0 996,325 + LRK53_RS04280 0.68 -1.0 996,325 + LRK53_RS04280 0.68 +0.4 996,325 + LRK53_RS04280 0.68 -1.6 996,325 + LRK53_RS04280 0.68 -0.5 996,325 + LRK53_RS04280 0.68 -0.6 996,325 + LRK53_RS04280 0.68 +0.2 996,326 - LRK53_RS04280 0.68 -0.8 996,326 - LRK53_RS04280 0.68 +3.3 996,326 - LRK53_RS04280 0.68 +0.2 996,326 - LRK53_RS04280 0.68 +1.0 996,326 - LRK53_RS04280 0.68 +1.0 996,326 - LRK53_RS04280 0.68 +1.2 996,326 - LRK53_RS04280 0.68 -0.6 996,326 - LRK53_RS04280 0.68 +1.0 996,326 - LRK53_RS04280 0.68 -1.8 996,328 + LRK53_RS04280 0.68 -0.0 996,328 + LRK53_RS04280 0.68 -0.8 996,328 + LRK53_RS04280 0.68 +0.2 996,328 + LRK53_RS04280 0.68 -0.5 996,328 + LRK53_RS04280 0.68 -1.1 996,329 - LRK53_RS04280 0.68 -0.8 996,329 - LRK53_RS04280 0.68 -1.0 996,329 - LRK53_RS04280 0.68 -1.1 996,329 - LRK53_RS04280 0.68 +1.0 996,531 + -0.6 996,531 + -0.2 996,531 + -0.5 996,531 + -2.4 996,531 + -0.3 996,531 + +1.0 996,531 + +0.1 996,531 + +1.0 996,531 + -0.2 996,531 + -1.2 996,531 + -0.0 996,531 + +0.3 996,531 + +0.4 996,531 + +0.4 996,531 + -0.0 996,531 + -0.0 996,531 + +1.0 996,531 + -0.2 996,531 + +1.0 996,531 + +2.3 996,531 + -0.3 996,531 + -0.6 996,531 + -0.6 996,531 + -3.1 996,531 + -1.0 996,531 + -2.4 996,531 + +0.4 996,531 + -1.1 996,532 - -1.3 996,532 - -1.2 996,532 - -1.0 996,532 - -0.6 996,532 - +1.0 996,532 - -0.6 996,532 - -0.7 996,532 - -1.4 996,532 - +0.3 996,532 - -0.6 996,532 - -1.4 996,532 - +1.5 996,532 - -1.0 996,532 - +0.5 996,532 - -1.0 996,532 - -0.8 996,532 - -1.4 996,532 - -0.8 996,532 - -0.0 996,532 - +0.4 996,532 - -0.0 996,532 - +0.4 996,532 - -0.5 996,532 - +0.2 996,532 - -0.0 996,532 - +1.0 996,532 - -0.0 996,532 - -0.6 996,532 - -0.0 996,532 - -1.8 996,532 - +2.0 996,532 - -0.0 996,532 - -1.0 996,532 - -0.6 996,532 - +1.0 996,532 - -2.2 996,532 - -0.6 996,532 - -0.6 996,621 + -1.8 996,621 + +0.6 996,621 + +1.4 996,621 + -1.0 996,621 + +0.4 996,621 + -1.4 996,621 + +1.0 996,621 + +0.2 996,621 + -1.0 996,621 + -0.4 996,621 + +1.0 996,621 + +0.5 996,621 + -0.7 996,621 + +0.1 996,621 + -1.0 996,622 - -1.0 996,622 - -2.6 996,622 - -2.6 996,622 - -0.0 996,622 - +1.0 996,622 - +1.0 996,622 - -1.4 996,622 - -0.0 996,622 - +0.4 996,622 - -1.2 996,622 - -0.7 996,622 - -1.6 996,622 - -0.5 996,622 - -1.0 996,622 - -1.0 996,622 - -1.0 996,622 - -1.0 996,645 + -0.6 996,645 + +0.4 996,645 + +2.5 996,645 + +2.0 996,645 + +1.0 996,645 + +1.5 996,645 + -1.4 996,646 - +0.3 996,646 - -0.4 996,646 - -1.4 996,646 - -0.6 996,646 - +0.4
Or see this region's nucleotide sequence