Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04185 and LRK53_RS04190 are separated by 15 nucleotides LRK53_RS04190 and LRK53_RS04195 are separated by 49 nucleotides LRK53_RS04195 and LRK53_RS04200 are separated by 78 nucleotides
LRK53_RS04185: LRK53_RS04185 - dihydrolipoyllysine-residue acetyltransferase, at 978,276 to 979,691
_RS04185
LRK53_RS04190: LRK53_RS04190 - dihydrolipoyl dehydrogenase, at 979,707 to 981,521
_RS04190
LRK53_RS04195: LRK53_RS04195 - 4-hydroxy-tetrahydrodipicolinate reductase, at 981,571 to 982,371
_RS04195
LRK53_RS04200: LRK53_RS04200 - hypothetical protein, at 982,450 to 982,926
_RS04200
Position (kb) 979
980
981
982 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 978.786 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.599 kb on + strand at 979.600 kb on - strand at 979.600 kb on - strand at 980.157 kb on + strand, within LRK53_RS04190 at 980.214 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.860 kb on - strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.477 kb on + strand at 981.477 kb on + strand at 981.477 kb on + strand at 981.477 kb on + strand at 981.478 kb on - strand at 981.478 kb on - strand at 981.501 kb on + strand at 981.501 kb on + strand at 981.501 kb on + strand at 981.501 kb on + strand at 981.502 kb on - strand at 981.502 kb on - strand at 981.502 kb on - strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.739 kb on + strand, within LRK53_RS04195 at 981.739 kb on + strand, within LRK53_RS04195 at 981.739 kb on + strand, within LRK53_RS04195 at 981.740 kb on - strand, within LRK53_RS04195 at 981.740 kb on - strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 982.025 kb on - strand, within LRK53_RS04195 at 982.025 kb on - strand, within LRK53_RS04195 at 982.285 kb on + strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.370 kb on - strand at 982.370 kb on - strand at 982.370 kb on - strand at 982.370 kb on - strand at 982.451 kb on + strand at 982.451 kb on + strand at 982.451 kb on + strand at 982.451 kb on + strand at 982.452 kb on - strand at 982.452 kb on - strand at 982.452 kb on - strand at 982.452 kb on - strand at 982.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 978,786 + LRK53_RS04185 0.36 -1.0 978,894 + LRK53_RS04185 0.44 -2.2 978,894 + LRK53_RS04185 0.44 -1.0 978,894 + LRK53_RS04185 0.44 -1.6 978,894 + LRK53_RS04185 0.44 -1.6 978,894 + LRK53_RS04185 0.44 -0.0 978,894 + LRK53_RS04185 0.44 -0.0 978,894 + LRK53_RS04185 0.44 +1.0 978,894 + LRK53_RS04185 0.44 -0.0 978,894 + LRK53_RS04185 0.44 -0.8 978,895 - LRK53_RS04185 0.44 -1.4 978,895 - LRK53_RS04185 0.44 +1.0 978,895 - LRK53_RS04185 0.44 -2.0 978,895 - LRK53_RS04185 0.44 -2.0 978,895 - LRK53_RS04185 0.44 -0.0 978,895 - LRK53_RS04185 0.44 -0.6 979,476 + LRK53_RS04185 0.85 -0.0 979,476 + LRK53_RS04185 0.85 -0.9 979,476 + LRK53_RS04185 0.85 +1.0 979,476 + LRK53_RS04185 0.85 -2.6 979,476 + LRK53_RS04185 0.85 -1.0 979,477 - LRK53_RS04185 0.85 +1.0 979,477 - LRK53_RS04185 0.85 -0.6 979,477 - LRK53_RS04185 0.85 -0.0 979,599 + -3.0 979,600 - -0.0 979,600 - -1.2 980,157 + LRK53_RS04190 0.25 -2.5 980,214 + LRK53_RS04190 0.28 -1.6 980,682 + LRK53_RS04190 0.54 -1.0 980,682 + LRK53_RS04190 0.54 -1.4 980,682 + LRK53_RS04190 0.54 -0.0 980,683 - LRK53_RS04190 0.54 -1.2 980,683 - LRK53_RS04190 0.54 -1.0 980,683 - LRK53_RS04190 0.54 +1.0 980,860 - LRK53_RS04190 0.64 +1.0 981,027 + LRK53_RS04190 0.73 -2.0 981,027 + LRK53_RS04190 0.73 -1.9 981,027 + LRK53_RS04190 0.73 -1.6 981,027 + LRK53_RS04190 0.73 +1.0 981,027 + LRK53_RS04190 0.73 -1.6 981,027 + LRK53_RS04190 0.73 -0.2 981,027 + LRK53_RS04190 0.73 -1.2 981,027 + LRK53_RS04190 0.73 -1.9 981,027 + LRK53_RS04190 0.73 -0.0 981,027 + LRK53_RS04190 0.73 -1.0 981,027 + LRK53_RS04190 0.73 -0.6 981,027 + LRK53_RS04190 0.73 -1.6 981,028 - LRK53_RS04190 0.73 -0.0 981,028 - LRK53_RS04190 0.73 -0.6 981,028 - LRK53_RS04190 0.73 -2.9 981,028 - LRK53_RS04190 0.73 +1.0 981,028 - LRK53_RS04190 0.73 -1.6 981,028 - LRK53_RS04190 0.73 -0.0 981,028 - LRK53_RS04190 0.73 -2.1 981,028 - LRK53_RS04190 0.73 -0.9 981,028 - LRK53_RS04190 0.73 -0.0 981,028 - LRK53_RS04190 0.73 -0.6 981,028 - LRK53_RS04190 0.73 -1.0 981,028 - LRK53_RS04190 0.73 -2.4 981,028 - LRK53_RS04190 0.73 -1.4 981,028 - LRK53_RS04190 0.73 -0.9 981,028 - LRK53_RS04190 0.73 -1.6 981,028 - LRK53_RS04190 0.73 -0.6 981,477 + -0.0 981,477 + -0.6 981,477 + +0.1 981,477 + -1.4 981,478 - -2.7 981,478 - -0.4 981,501 + -0.0 981,501 + +1.0 981,501 + +0.3 981,501 + -0.6 981,502 - -0.6 981,502 - -0.9 981,502 - -0.6 981,584 + -0.4 981,584 + +1.0 981,584 + -0.0 981,584 + -1.0 981,584 + +0.3 981,584 + -0.2 981,584 + -1.9 981,584 + -0.0 981,584 + -1.3 981,584 + +0.2 981,584 + +0.3 981,584 + -0.9 981,584 + -1.0 981,584 + -1.5 981,584 + -1.2 981,584 + -1.0 981,584 + -0.6 981,584 + -1.0 981,584 + +1.4 981,584 + -1.2 981,584 + +0.2 981,584 + -1.6 981,584 + +1.0 981,584 + -0.6 981,584 + -1.4 981,584 + +1.0 981,584 + -0.9 981,584 + +1.0 981,584 + -1.4 981,584 + -0.0 981,584 + -0.6 981,584 + -1.2 981,584 + -0.0 981,584 + -2.4 981,584 + -0.6 981,584 + +1.0 981,584 + +2.5 981,584 + -0.6 981,584 + +0.1 981,584 + +0.4 981,584 + -0.4 981,585 - -1.0 981,585 - +1.0 981,585 - -0.0 981,585 - -0.4 981,585 - -2.0 981,585 - -0.6 981,585 - +0.4 981,585 - -0.6 981,585 - -0.0 981,585 - -1.4 981,585 - -2.0 981,585 - -1.4 981,585 - -1.5 981,585 - -0.6 981,585 - -0.6 981,585 - -0.8 981,585 - -2.0 981,585 - -1.6 981,585 - +1.0 981,585 - -0.6 981,585 - -0.0 981,585 - -1.0 981,585 - +0.1 981,585 - -0.2 981,585 - -0.6 981,585 - -1.0 981,585 - +0.1 981,585 - -1.0 981,585 - +0.3 981,585 - -1.2 981,585 - -0.0 981,739 + LRK53_RS04195 0.21 -1.4 981,739 + LRK53_RS04195 0.21 -0.3 981,739 + LRK53_RS04195 0.21 +0.0 981,740 - LRK53_RS04195 0.21 -0.8 981,740 - LRK53_RS04195 0.21 -0.7 981,779 + LRK53_RS04195 0.26 -0.0 981,779 + LRK53_RS04195 0.26 -2.0 981,779 + LRK53_RS04195 0.26 +1.0 981,779 + LRK53_RS04195 0.26 -0.4 981,779 + LRK53_RS04195 0.26 +1.0 981,779 + LRK53_RS04195 0.26 +0.3 981,779 + LRK53_RS04195 0.26 +0.5 981,779 + LRK53_RS04195 0.26 -0.1 981,779 + LRK53_RS04195 0.26 -1.4 981,779 + LRK53_RS04195 0.26 -1.6 981,779 + LRK53_RS04195 0.26 -1.9 981,779 + LRK53_RS04195 0.26 -1.6 981,779 + LRK53_RS04195 0.26 -0.4 981,779 + LRK53_RS04195 0.26 -0.5 981,779 + LRK53_RS04195 0.26 -0.0 981,779 + LRK53_RS04195 0.26 -1.6 981,779 + LRK53_RS04195 0.26 -0.7 981,779 + LRK53_RS04195 0.26 -0.6 981,780 - LRK53_RS04195 0.26 -1.4 981,780 - LRK53_RS04195 0.26 -1.0 981,780 - LRK53_RS04195 0.26 -0.4 981,780 - LRK53_RS04195 0.26 +0.7 981,780 - LRK53_RS04195 0.26 -0.8 981,780 - LRK53_RS04195 0.26 -1.2 981,780 - LRK53_RS04195 0.26 -1.0 981,780 - LRK53_RS04195 0.26 -1.9 981,780 - LRK53_RS04195 0.26 -0.7 981,780 - LRK53_RS04195 0.26 +1.0 981,780 - LRK53_RS04195 0.26 -0.8 981,780 - LRK53_RS04195 0.26 -1.5 981,780 - LRK53_RS04195 0.26 +0.1 981,780 - LRK53_RS04195 0.26 +0.4 981,780 - LRK53_RS04195 0.26 -0.6 981,780 - LRK53_RS04195 0.26 -0.2 981,780 - LRK53_RS04195 0.26 -0.4 981,790 + LRK53_RS04195 0.27 -1.1 981,790 + LRK53_RS04195 0.27 -0.4 981,790 + LRK53_RS04195 0.27 -0.5 981,790 + LRK53_RS04195 0.27 -0.8 981,790 + LRK53_RS04195 0.27 -1.0 981,790 + LRK53_RS04195 0.27 -0.2 981,790 + LRK53_RS04195 0.27 +2.0 981,790 + LRK53_RS04195 0.27 -1.0 981,790 + LRK53_RS04195 0.27 +0.4 981,791 - LRK53_RS04195 0.27 -0.6 981,791 - LRK53_RS04195 0.27 +2.0 981,791 - LRK53_RS04195 0.27 -0.4 981,791 - LRK53_RS04195 0.27 +2.5 981,791 - LRK53_RS04195 0.27 -1.0 981,791 - LRK53_RS04195 0.27 +1.0 981,791 - LRK53_RS04195 0.27 +0.1 981,791 - LRK53_RS04195 0.27 +1.0 981,886 + LRK53_RS04195 0.39 -0.0 981,886 + LRK53_RS04195 0.39 -1.9 981,886 + LRK53_RS04195 0.39 +0.1 981,886 + LRK53_RS04195 0.39 +0.2 981,886 + LRK53_RS04195 0.39 -1.5 981,886 + LRK53_RS04195 0.39 +0.1 981,887 - LRK53_RS04195 0.39 -0.7 981,887 - LRK53_RS04195 0.39 -1.7 981,887 - LRK53_RS04195 0.39 -0.0 981,887 - LRK53_RS04195 0.39 +0.2 981,887 - LRK53_RS04195 0.39 +1.0 981,887 - LRK53_RS04195 0.39 +0.2 981,887 - LRK53_RS04195 0.39 -1.4 981,887 - LRK53_RS04195 0.39 +0.4 981,887 - LRK53_RS04195 0.39 +1.4 982,025 - LRK53_RS04195 0.57 -1.3 982,025 - LRK53_RS04195 0.57 +3.0 982,285 + LRK53_RS04195 0.89 +0.2 982,286 - LRK53_RS04195 0.89 +0.5 982,286 - LRK53_RS04195 0.89 -0.6 982,286 - LRK53_RS04195 0.89 -0.0 982,286 - LRK53_RS04195 0.89 +0.4 982,286 - LRK53_RS04195 0.89 +0.4 982,369 + -2.0 982,369 + -1.2 982,369 + +2.0 982,369 + -0.0 982,369 + -0.0 982,369 + +1.0 982,370 - -0.7 982,370 - +2.0 982,370 - +1.0 982,370 - +1.0 982,451 + -0.0 982,451 + -1.3 982,451 + -0.6 982,451 + -1.4 982,452 - -1.4 982,452 - +0.5 982,452 - -2.0 982,452 - -1.0 982,452 - -1.2
Or see this region's nucleotide sequence