Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04180 and LRK53_RS04185 are separated by 227 nucleotides LRK53_RS04185 and LRK53_RS04190 are separated by 15 nucleotides
LRK53_RS04180: LRK53_RS04180 - ABC transporter ATP-binding protein, at 977,164 to 978,048
_RS04180
LRK53_RS04185: LRK53_RS04185 - dihydrolipoyllysine-residue acetyltransferase, at 978,276 to 979,691
_RS04185
LRK53_RS04190: LRK53_RS04190 - dihydrolipoyl dehydrogenase, at 979,707 to 981,521
_RS04190
Position (kb) 978
979
980 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.648 kb on + strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.649 kb on - strand, within LRK53_RS04180 at 977.730 kb on + strand, within LRK53_RS04180 at 977.731 kb on - strand, within LRK53_RS04180 at 977.787 kb on + strand, within LRK53_RS04180 at 977.788 kb on - strand, within LRK53_RS04180 at 977.788 kb on - strand, within LRK53_RS04180 at 977.788 kb on - strand, within LRK53_RS04180 at 977.792 kb on + strand, within LRK53_RS04180 at 977.792 kb on + strand, within LRK53_RS04180 at 977.793 kb on - strand, within LRK53_RS04180 at 978.017 kb on + strand at 978.017 kb on + strand at 978.018 kb on - strand at 978.018 kb on - strand at 978.018 kb on - strand at 978.037 kb on + strand at 978.038 kb on - strand at 978.038 kb on - strand at 978.038 kb on - strand at 978.096 kb on - strand at 978.138 kb on + strand at 978.138 kb on + strand at 978.139 kb on - strand at 978.139 kb on - strand at 978.211 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.227 kb on + strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.228 kb on - strand at 978.252 kb on - strand at 978.500 kb on + strand, within LRK53_RS04185 at 978.501 kb on - strand, within LRK53_RS04185 at 978.502 kb on + strand, within LRK53_RS04185 at 978.502 kb on + strand, within LRK53_RS04185 at 978.502 kb on + strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.503 kb on - strand, within LRK53_RS04185 at 978.504 kb on + strand, within LRK53_RS04185 at 978.518 kb on + strand, within LRK53_RS04185 at 978.518 kb on + strand, within LRK53_RS04185 at 978.786 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.599 kb on + strand at 979.600 kb on - strand at 979.600 kb on - strand at 980.157 kb on + strand, within LRK53_RS04190 at 980.214 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 977,648 + LRK53_RS04180 0.55 -1.3 977,648 + LRK53_RS04180 0.55 -2.3 977,648 + LRK53_RS04180 0.55 +1.0 977,648 + LRK53_RS04180 0.55 -0.0 977,648 + LRK53_RS04180 0.55 +1.2 977,649 - LRK53_RS04180 0.55 -1.3 977,649 - LRK53_RS04180 0.55 +2.0 977,649 - LRK53_RS04180 0.55 -0.0 977,649 - LRK53_RS04180 0.55 -1.3 977,649 - LRK53_RS04180 0.55 -1.0 977,730 + LRK53_RS04180 0.64 -1.3 977,731 - LRK53_RS04180 0.64 +1.4 977,787 + LRK53_RS04180 0.70 +1.2 977,788 - LRK53_RS04180 0.71 -0.0 977,788 - LRK53_RS04180 0.71 -1.0 977,788 - LRK53_RS04180 0.71 -1.0 977,792 + LRK53_RS04180 0.71 -0.8 977,792 + LRK53_RS04180 0.71 +2.0 977,793 - LRK53_RS04180 0.71 -0.6 978,017 + -2.3 978,017 + +0.2 978,018 - -0.6 978,018 - -1.5 978,018 - -0.7 978,037 + -1.6 978,038 - -1.1 978,038 - -0.6 978,038 - -0.2 978,096 - -0.0 978,138 + -0.2 978,138 + +0.6 978,139 - -0.0 978,139 - -1.6 978,211 + -1.5 978,227 + +0.2 978,227 + -1.6 978,227 + +2.0 978,227 + -0.3 978,227 + -1.6 978,227 + -1.6 978,227 + -1.2 978,227 + -1.3 978,227 + -0.3 978,227 + -1.6 978,227 + -1.3 978,228 - -0.7 978,228 - -1.6 978,228 - +1.0 978,228 - -0.6 978,228 - -1.8 978,228 - -1.3 978,228 - -1.0 978,252 - -0.6 978,500 + LRK53_RS04185 0.16 -0.6 978,501 - LRK53_RS04185 0.16 +1.0 978,502 + LRK53_RS04185 0.16 +1.0 978,502 + LRK53_RS04185 0.16 -1.7 978,502 + LRK53_RS04185 0.16 -1.6 978,503 - LRK53_RS04185 0.16 -1.3 978,503 - LRK53_RS04185 0.16 -0.0 978,503 - LRK53_RS04185 0.16 -1.3 978,503 - LRK53_RS04185 0.16 -0.0 978,503 - LRK53_RS04185 0.16 -2.0 978,504 + LRK53_RS04185 0.16 +0.4 978,518 + LRK53_RS04185 0.17 -1.0 978,518 + LRK53_RS04185 0.17 -2.4 978,786 + LRK53_RS04185 0.36 -1.0 978,894 + LRK53_RS04185 0.44 -2.2 978,894 + LRK53_RS04185 0.44 -1.0 978,894 + LRK53_RS04185 0.44 -1.6 978,894 + LRK53_RS04185 0.44 -1.6 978,894 + LRK53_RS04185 0.44 -0.0 978,894 + LRK53_RS04185 0.44 -0.0 978,894 + LRK53_RS04185 0.44 +1.0 978,894 + LRK53_RS04185 0.44 -0.0 978,894 + LRK53_RS04185 0.44 -0.8 978,895 - LRK53_RS04185 0.44 +1.0 978,895 - LRK53_RS04185 0.44 -2.0 978,895 - LRK53_RS04185 0.44 -2.0 978,895 - LRK53_RS04185 0.44 -0.0 978,895 - LRK53_RS04185 0.44 -0.6 978,895 - LRK53_RS04185 0.44 -0.6 979,476 + LRK53_RS04185 0.85 -0.0 979,476 + LRK53_RS04185 0.85 -0.8 979,476 + LRK53_RS04185 0.85 +1.0 979,476 + LRK53_RS04185 0.85 -2.6 979,476 + LRK53_RS04185 0.85 -1.0 979,477 - LRK53_RS04185 0.85 +1.0 979,477 - LRK53_RS04185 0.85 -0.6 979,477 - LRK53_RS04185 0.85 -0.0 979,599 + -2.9 979,600 - -0.0 979,600 - -1.1 980,157 + LRK53_RS04190 0.25 -2.5 980,214 + LRK53_RS04190 0.28 -1.6 980,682 + LRK53_RS04190 0.54 -1.0 980,682 + LRK53_RS04190 0.54 -0.0 980,683 - LRK53_RS04190 0.54 -1.2 980,683 - LRK53_RS04190 0.54 -1.0 980,683 - LRK53_RS04190 0.54 +1.0
Or see this region's nucleotide sequence