Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03800 and LRK53_RS03805 overlap by 4 nucleotides LRK53_RS03805 and LRK53_RS03810 are separated by 4 nucleotides LRK53_RS03810 and LRK53_RS03815 overlap by 4 nucleotides LRK53_RS03815 and LRK53_RS03820 overlap by 4 nucleotides LRK53_RS03820 and LRK53_RS03825 overlap by 4 nucleotides
LRK53_RS03800: LRK53_RS03800 - FAD-dependent oxidoreductase, at 892,846 to 893,841
_RS03800
LRK53_RS03805: LRK53_RS03805 - sulfur carrier protein ThiS, at 893,838 to 894,032
_RS03805
LRK53_RS03810: LRK53_RS03810 - thiazole synthase, at 894,037 to 894,816
_RS03810
LRK53_RS03815: LRK53_RS03815 - thiamine phosphate synthase, at 894,813 to 895,421
_RS03815
LRK53_RS03820: LRK53_RS03820 - ThiF family adenylyltransferase, at 895,418 to 896,395
_RS03820
LRK53_RS03825: LRK53_RS03825 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 896,392 to 897,186
_RS03825
Position (kb)
894
895
896 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 894.030 kb on + strand at 894.030 kb on + strand at 894.030 kb on + strand at 894.030 kb on + strand at 894.030 kb on + strand at 894.030 kb on + strand at 894.030 kb on + strand at 894.031 kb on - strand at 894.055 kb on + strand at 894.101 kb on - strand at 894.101 kb on - strand at 894.161 kb on + strand, within LRK53_RS03810 at 894.161 kb on + strand, within LRK53_RS03810 at 894.162 kb on - strand, within LRK53_RS03810 at 894.162 kb on - strand, within LRK53_RS03810 at 894.430 kb on + strand, within LRK53_RS03810 at 894.430 kb on + strand, within LRK53_RS03810 at 894.430 kb on + strand, within LRK53_RS03810 at 894.430 kb on + strand, within LRK53_RS03810 at 894.430 kb on + strand, within LRK53_RS03810 at 894.430 kb on + strand, within LRK53_RS03810 at 894.431 kb on - strand, within LRK53_RS03810 at 894.431 kb on - strand, within LRK53_RS03810 at 894.431 kb on - strand, within LRK53_RS03810 at 894.431 kb on - strand, within LRK53_RS03810 at 894.431 kb on - strand, within LRK53_RS03810 at 894.431 kb on - strand, within LRK53_RS03810 at 894.640 kb on + strand, within LRK53_RS03810 at 894.640 kb on + strand, within LRK53_RS03810 at 894.640 kb on + strand, within LRK53_RS03810 at 894.641 kb on - strand, within LRK53_RS03810 at 894.641 kb on - strand, within LRK53_RS03810 at 894.834 kb on + strand at 894.834 kb on + strand at 894.835 kb on - strand at 894.835 kb on - strand at 894.835 kb on - strand at 895.032 kb on + strand, within LRK53_RS03815 at 895.032 kb on + strand, within LRK53_RS03815 at 895.032 kb on + strand, within LRK53_RS03815 at 895.032 kb on + strand, within LRK53_RS03815 at 895.032 kb on + strand, within LRK53_RS03815 at 895.032 kb on + strand, within LRK53_RS03815 at 895.033 kb on - strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.161 kb on + strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.162 kb on - strand, within LRK53_RS03815 at 895.626 kb on - strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.698 kb on + strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.699 kb on - strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.802 kb on + strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 895.803 kb on - strand, within LRK53_RS03820 at 896.350 kb on + strand at 896.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 894,030 + -0.2 894,030 + -2.2 894,030 + +1.0 894,030 + -0.3 894,030 + -1.6 894,030 + -0.2 894,030 + -1.3 894,031 - -1.3 894,055 + -0.8 894,101 - -0.6 894,101 - +1.6 894,161 + LRK53_RS03810 0.16 +1.0 894,161 + LRK53_RS03810 0.16 -1.0 894,162 - LRK53_RS03810 0.16 -1.3 894,162 - LRK53_RS03810 0.16 -0.6 894,430 + LRK53_RS03810 0.50 +0.1 894,430 + LRK53_RS03810 0.50 -1.6 894,430 + LRK53_RS03810 0.50 -0.6 894,430 + LRK53_RS03810 0.50 +0.2 894,430 + LRK53_RS03810 0.50 -0.3 894,430 + LRK53_RS03810 0.50 +1.0 894,431 - LRK53_RS03810 0.51 +1.6 894,431 - LRK53_RS03810 0.51 -1.4 894,431 - LRK53_RS03810 0.51 +2.6 894,431 - LRK53_RS03810 0.51 -0.0 894,431 - LRK53_RS03810 0.51 -1.0 894,431 - LRK53_RS03810 0.51 +1.0 894,640 + LRK53_RS03810 0.77 -1.5 894,640 + LRK53_RS03810 0.77 -1.0 894,640 + LRK53_RS03810 0.77 -0.0 894,641 - LRK53_RS03810 0.77 -2.7 894,641 - LRK53_RS03810 0.77 +0.5 894,834 + -0.0 894,834 + +1.0 894,835 - -1.8 894,835 - -1.8 894,835 - +1.2 895,032 + LRK53_RS03815 0.36 -1.3 895,032 + LRK53_RS03815 0.36 -1.6 895,032 + LRK53_RS03815 0.36 -0.0 895,032 + LRK53_RS03815 0.36 -0.8 895,032 + LRK53_RS03815 0.36 -0.6 895,032 + LRK53_RS03815 0.36 -1.8 895,033 - LRK53_RS03815 0.36 -0.8 895,161 + LRK53_RS03815 0.57 -2.3 895,161 + LRK53_RS03815 0.57 +1.6 895,161 + LRK53_RS03815 0.57 -2.6 895,161 + LRK53_RS03815 0.57 -0.6 895,161 + LRK53_RS03815 0.57 +1.0 895,161 + LRK53_RS03815 0.57 -2.0 895,161 + LRK53_RS03815 0.57 +0.7 895,161 + LRK53_RS03815 0.57 -0.2 895,161 + LRK53_RS03815 0.57 +1.0 895,161 + LRK53_RS03815 0.57 -0.8 895,161 + LRK53_RS03815 0.57 -0.0 895,162 - LRK53_RS03815 0.57 -0.6 895,162 - LRK53_RS03815 0.57 +3.0 895,162 - LRK53_RS03815 0.57 -1.0 895,162 - LRK53_RS03815 0.57 -2.6 895,162 - LRK53_RS03815 0.57 +0.0 895,162 - LRK53_RS03815 0.57 -2.0 895,162 - LRK53_RS03815 0.57 -1.5 895,162 - LRK53_RS03815 0.57 -0.6 895,162 - LRK53_RS03815 0.57 -0.8 895,162 - LRK53_RS03815 0.57 -0.5 895,162 - LRK53_RS03815 0.57 -2.0 895,626 - LRK53_RS03820 0.21 -1.3 895,698 + LRK53_RS03820 0.29 -1.2 895,698 + LRK53_RS03820 0.29 -0.0 895,698 + LRK53_RS03820 0.29 -1.9 895,698 + LRK53_RS03820 0.29 -0.6 895,698 + LRK53_RS03820 0.29 -0.3 895,698 + LRK53_RS03820 0.29 -0.6 895,698 + LRK53_RS03820 0.29 +1.0 895,698 + LRK53_RS03820 0.29 -0.6 895,698 + LRK53_RS03820 0.29 -0.0 895,699 - LRK53_RS03820 0.29 +1.0 895,699 - LRK53_RS03820 0.29 -1.0 895,699 - LRK53_RS03820 0.29 -2.0 895,699 - LRK53_RS03820 0.29 +0.8 895,699 - LRK53_RS03820 0.29 -0.0 895,699 - LRK53_RS03820 0.29 -1.6 895,699 - LRK53_RS03820 0.29 -0.2 895,699 - LRK53_RS03820 0.29 +1.7 895,802 + LRK53_RS03820 0.39 -0.0 895,802 + LRK53_RS03820 0.39 +1.0 895,802 + LRK53_RS03820 0.39 -0.3 895,802 + LRK53_RS03820 0.39 -2.0 895,802 + LRK53_RS03820 0.39 -0.3 895,802 + LRK53_RS03820 0.39 -1.0 895,802 + LRK53_RS03820 0.39 +0.2 895,802 + LRK53_RS03820 0.39 +0.4 895,802 + LRK53_RS03820 0.39 +0.2 895,802 + LRK53_RS03820 0.39 -1.3 895,802 + LRK53_RS03820 0.39 -1.0 895,802 + LRK53_RS03820 0.39 -0.9 895,803 - LRK53_RS03820 0.39 -0.0 895,803 - LRK53_RS03820 0.39 -1.1 895,803 - LRK53_RS03820 0.39 -0.6 895,803 - LRK53_RS03820 0.39 -0.5 895,803 - LRK53_RS03820 0.39 -2.5 895,803 - LRK53_RS03820 0.39 -0.0 895,803 - LRK53_RS03820 0.39 -0.6 895,803 - LRK53_RS03820 0.39 -0.0 896,350 + -2.9 896,350 + -1.0
Or see this region's nucleotide sequence