Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03765 and LRK53_RS03770 are separated by 3 nucleotides LRK53_RS03770 and LRK53_RS03775 are separated by 55 nucleotides LRK53_RS03775 and LRK53_RS03780 are separated by 2 nucleotides
LRK53_RS03765: LRK53_RS03765 - NAD-dependent DNA ligase LigB, at 886,043 to 887,728
_RS03765
LRK53_RS03770: LRK53_RS03770 - hypothetical protein, at 887,732 to 888,133
_RS03770
LRK53_RS03775: LRK53_RS03775 - DUF938 domain-containing protein, at 888,189 to 888,785
_RS03775
LRK53_RS03780: LRK53_RS03780 - DUF4325 domain-containing protein, at 888,788 to 889,849
_RS03780
Position (kb) 887
888
889 Strain fitness (log2 ratio) -2
-1
0
1
2 at 886.808 kb on + strand, within LRK53_RS03765 at 886.808 kb on + strand, within LRK53_RS03765 at 886.808 kb on + strand, within LRK53_RS03765 at 886.809 kb on - strand, within LRK53_RS03765 at 886.809 kb on - strand, within LRK53_RS03765 at 886.862 kb on + strand, within LRK53_RS03765 at 886.973 kb on + strand, within LRK53_RS03765 at 887.251 kb on - strand, within LRK53_RS03765 at 887.365 kb on + strand, within LRK53_RS03765 at 887.365 kb on + strand, within LRK53_RS03765 at 887.757 kb on + strand at 887.757 kb on + strand at 887.757 kb on + strand at 887.757 kb on + strand at 887.757 kb on + strand at 887.758 kb on - strand at 887.758 kb on - strand at 887.758 kb on - strand at 887.758 kb on - strand at 887.758 kb on - strand at 887.759 kb on + strand at 887.760 kb on + strand at 887.760 kb on + strand at 887.760 kb on + strand at 887.760 kb on + strand at 887.760 kb on + strand at 887.760 kb on + strand at 887.761 kb on - strand at 887.761 kb on - strand at 887.761 kb on - strand at 887.979 kb on + strand, within LRK53_RS03770 at 887.979 kb on + strand, within LRK53_RS03770 at 887.979 kb on + strand, within LRK53_RS03770 at 887.979 kb on + strand, within LRK53_RS03770 at 887.980 kb on - strand, within LRK53_RS03770 at 887.980 kb on - strand, within LRK53_RS03770 at 887.980 kb on - strand, within LRK53_RS03770 at 887.980 kb on - strand, within LRK53_RS03770 at 887.980 kb on - strand, within LRK53_RS03770 at 887.980 kb on - strand, within LRK53_RS03770 at 888.000 kb on + strand, within LRK53_RS03770 at 888.000 kb on + strand, within LRK53_RS03770 at 888.000 kb on + strand, within LRK53_RS03770 at 888.000 kb on + strand, within LRK53_RS03770 at 888.000 kb on + strand, within LRK53_RS03770 at 888.001 kb on - strand, within LRK53_RS03770 at 888.001 kb on - strand, within LRK53_RS03770 at 888.001 kb on - strand, within LRK53_RS03770 at 888.001 kb on - strand, within LRK53_RS03770 at 888.001 kb on - strand, within LRK53_RS03770 at 888.081 kb on + strand, within LRK53_RS03770 at 888.082 kb on - strand, within LRK53_RS03770 at 888.144 kb on + strand at 888.144 kb on + strand at 888.144 kb on + strand at 888.145 kb on - strand at 888.145 kb on - strand at 888.391 kb on + strand, within LRK53_RS03775 at 888.391 kb on + strand, within LRK53_RS03775 at 888.391 kb on + strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.392 kb on - strand, within LRK53_RS03775 at 888.769 kb on + strand at 888.769 kb on + strand at 888.770 kb on - strand at 888.770 kb on - strand at 888.770 kb on - strand at 888.854 kb on + strand at 888.854 kb on + strand at 888.854 kb on + strand at 888.854 kb on + strand at 888.854 kb on + strand at 889.089 kb on + strand, within LRK53_RS03780 at 889.089 kb on + strand, within LRK53_RS03780 at 889.089 kb on + strand, within LRK53_RS03780 at 889.089 kb on + strand, within LRK53_RS03780 at 889.089 kb on + strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.090 kb on - strand, within LRK53_RS03780 at 889.110 kb on + strand, within LRK53_RS03780 at 889.110 kb on + strand, within LRK53_RS03780 at 889.110 kb on + strand, within LRK53_RS03780 at 889.110 kb on + strand, within LRK53_RS03780 at 889.110 kb on + strand, within LRK53_RS03780 at 889.111 kb on - strand, within LRK53_RS03780 at 889.111 kb on - strand, within LRK53_RS03780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 886,808 + LRK53_RS03765 0.45 -1.3 886,808 + LRK53_RS03765 0.45 +1.0 886,808 + LRK53_RS03765 0.45 +2.0 886,809 - LRK53_RS03765 0.45 -0.6 886,809 - LRK53_RS03765 0.45 -1.8 886,862 + LRK53_RS03765 0.49 +2.0 886,973 + LRK53_RS03765 0.55 -0.2 887,251 - LRK53_RS03765 0.72 -1.6 887,365 + LRK53_RS03765 0.78 -2.0 887,365 + LRK53_RS03765 0.78 -1.3 887,757 + -0.5 887,757 + +1.0 887,757 + -0.6 887,757 + -0.8 887,757 + -0.6 887,758 - -0.6 887,758 - +1.0 887,758 - +1.0 887,758 - -0.3 887,758 - -0.8 887,759 + -2.0 887,760 + +0.4 887,760 + +0.2 887,760 + -0.2 887,760 + +0.7 887,760 + -0.6 887,760 + -1.7 887,761 - -1.0 887,761 - -1.3 887,761 - -1.0 887,979 + LRK53_RS03770 0.61 -1.0 887,979 + LRK53_RS03770 0.61 +1.0 887,979 + LRK53_RS03770 0.61 -1.6 887,979 + LRK53_RS03770 0.61 -1.1 887,980 - LRK53_RS03770 0.62 -1.0 887,980 - LRK53_RS03770 0.62 -1.6 887,980 - LRK53_RS03770 0.62 -1.8 887,980 - LRK53_RS03770 0.62 -1.3 887,980 - LRK53_RS03770 0.62 -1.0 887,980 - LRK53_RS03770 0.62 -0.0 888,000 + LRK53_RS03770 0.67 -0.6 888,000 + LRK53_RS03770 0.67 -1.1 888,000 + LRK53_RS03770 0.67 -1.3 888,000 + LRK53_RS03770 0.67 -2.3 888,000 + LRK53_RS03770 0.67 -1.6 888,001 - LRK53_RS03770 0.67 +0.2 888,001 - LRK53_RS03770 0.67 -1.6 888,001 - LRK53_RS03770 0.67 +0.7 888,001 - LRK53_RS03770 0.67 -0.0 888,001 - LRK53_RS03770 0.67 -0.7 888,081 + LRK53_RS03770 0.87 -0.8 888,082 - LRK53_RS03770 0.87 +0.4 888,144 + +1.0 888,144 + -0.5 888,144 + -0.6 888,145 - +0.2 888,145 - +0.4 888,391 + LRK53_RS03775 0.34 -0.6 888,391 + LRK53_RS03775 0.34 +1.4 888,391 + LRK53_RS03775 0.34 -1.6 888,392 - LRK53_RS03775 0.34 -0.2 888,392 - LRK53_RS03775 0.34 -0.3 888,392 - LRK53_RS03775 0.34 -0.0 888,392 - LRK53_RS03775 0.34 -0.4 888,392 - LRK53_RS03775 0.34 +0.5 888,392 - LRK53_RS03775 0.34 +0.1 888,392 - LRK53_RS03775 0.34 -2.3 888,392 - LRK53_RS03775 0.34 -0.8 888,392 - LRK53_RS03775 0.34 -1.7 888,769 + +2.0 888,769 + -1.2 888,770 - -2.6 888,770 - +1.0 888,770 - -1.6 888,854 + -0.0 888,854 + -0.0 888,854 + +0.4 888,854 + +0.5 888,854 + +1.0 889,089 + LRK53_RS03780 0.28 +0.4 889,089 + LRK53_RS03780 0.28 -0.3 889,089 + LRK53_RS03780 0.28 +0.4 889,089 + LRK53_RS03780 0.28 -0.0 889,089 + LRK53_RS03780 0.28 -1.6 889,090 - LRK53_RS03780 0.28 -0.6 889,090 - LRK53_RS03780 0.28 -0.0 889,090 - LRK53_RS03780 0.28 -0.0 889,090 - LRK53_RS03780 0.28 -0.7 889,090 - LRK53_RS03780 0.28 -1.0 889,090 - LRK53_RS03780 0.28 -1.0 889,090 - LRK53_RS03780 0.28 -0.3 889,090 - LRK53_RS03780 0.28 -0.0 889,090 - LRK53_RS03780 0.28 -1.5 889,110 + LRK53_RS03780 0.30 -0.6 889,110 + LRK53_RS03780 0.30 +1.3 889,110 + LRK53_RS03780 0.30 -0.8 889,110 + LRK53_RS03780 0.30 -0.7 889,110 + LRK53_RS03780 0.30 -1.0 889,111 - LRK53_RS03780 0.30 +0.6 889,111 - LRK53_RS03780 0.30 -0.8
Or see this region's nucleotide sequence