Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03405 and LRK53_RS03410 overlap by 4 nucleotides LRK53_RS03410 and LRK53_RS03415 are separated by 121 nucleotides LRK53_RS03415 and LRK53_RS03420 are separated by 40 nucleotides
LRK53_RS03405: LRK53_RS03405 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 805,737 to 807,386
_RS03405
LRK53_RS03410: LRK53_RS03410 - SCP2 sterol-binding domain-containing protein, at 807,383 to 808,033
_RS03410
LRK53_RS03415: LRK53_RS03415 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 808,155 to 808,640
_RS03415
LRK53_RS03420: LRK53_RS03420 - hypothetical protein, at 808,681 to 808,884
_RS03420
Position (kb) 807
808
809 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 807.455 kb on + strand, within LRK53_RS03410 at 807.455 kb on + strand, within LRK53_RS03410 at 807.455 kb on + strand, within LRK53_RS03410 at 807.455 kb on + strand, within LRK53_RS03410 at 807.456 kb on - strand, within LRK53_RS03410 at 807.456 kb on - strand, within LRK53_RS03410 at 807.456 kb on - strand, within LRK53_RS03410 at 808.044 kb on + strand at 808.044 kb on + strand at 808.045 kb on - strand at 808.045 kb on - strand at 808.045 kb on - strand at 808.086 kb on + strand at 808.087 kb on - strand at 808.087 kb on - strand at 808.156 kb on + strand at 808.462 kb on + strand, within LRK53_RS03415 at 808.462 kb on + strand, within LRK53_RS03415 at 808.462 kb on + strand, within LRK53_RS03415 at 808.462 kb on + strand, within LRK53_RS03415 at 808.462 kb on + strand, within LRK53_RS03415 at 808.463 kb on - strand, within LRK53_RS03415 at 808.463 kb on - strand, within LRK53_RS03415 at 808.480 kb on + strand, within LRK53_RS03415 at 808.481 kb on - strand, within LRK53_RS03415 at 808.481 kb on - strand, within LRK53_RS03415 at 808.481 kb on - strand, within LRK53_RS03415 at 808.481 kb on - strand, within LRK53_RS03415 at 808.489 kb on + strand, within LRK53_RS03415 at 808.489 kb on + strand, within LRK53_RS03415 at 808.489 kb on + strand, within LRK53_RS03415 at 808.568 kb on + strand, within LRK53_RS03415 at 808.568 kb on + strand, within LRK53_RS03415 at 808.569 kb on - strand, within LRK53_RS03415 at 808.599 kb on - strand at 808.650 kb on - strand at 808.731 kb on + strand, within LRK53_RS03420 at 808.955 kb on + strand at 808.955 kb on + strand at 808.956 kb on - strand at 808.961 kb on + strand at 808.961 kb on + strand at 808.961 kb on + strand at 808.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 807,455 + LRK53_RS03410 0.11 -0.6 807,455 + LRK53_RS03410 0.11 -1.7 807,455 + LRK53_RS03410 0.11 -0.0 807,455 + LRK53_RS03410 0.11 -2.4 807,456 - LRK53_RS03410 0.11 -0.5 807,456 - LRK53_RS03410 0.11 +2.3 807,456 - LRK53_RS03410 0.11 -1.2 808,044 + -0.6 808,044 + -2.9 808,045 - -0.0 808,045 - -0.0 808,045 - +2.5 808,086 + -0.8 808,087 - -0.7 808,087 - -0.7 808,156 + -0.6 808,462 + LRK53_RS03415 0.63 +1.0 808,462 + LRK53_RS03415 0.63 -0.0 808,462 + LRK53_RS03415 0.63 -1.0 808,462 + LRK53_RS03415 0.63 +0.4 808,462 + LRK53_RS03415 0.63 -1.4 808,463 - LRK53_RS03415 0.63 +1.0 808,463 - LRK53_RS03415 0.63 -1.4 808,480 + LRK53_RS03415 0.67 -0.7 808,481 - LRK53_RS03415 0.67 -0.0 808,481 - LRK53_RS03415 0.67 -1.4 808,481 - LRK53_RS03415 0.67 -0.0 808,481 - LRK53_RS03415 0.67 -1.0 808,489 + LRK53_RS03415 0.69 -1.3 808,489 + LRK53_RS03415 0.69 -0.6 808,489 + LRK53_RS03415 0.69 -0.0 808,568 + LRK53_RS03415 0.85 -1.6 808,568 + LRK53_RS03415 0.85 +1.0 808,569 - LRK53_RS03415 0.85 +0.4 808,599 - +0.7 808,650 - -1.0 808,731 + LRK53_RS03420 0.25 -0.4 808,955 + -1.7 808,955 + -0.2 808,956 - -0.0 808,961 + -0.5 808,961 + -1.0 808,961 + -0.9 808,962 - -0.6
Or see this region's nucleotide sequence