Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03330 and LRK53_RS03335 are separated by 157 nucleotides LRK53_RS03335 and LRK53_RS03340 are separated by 208 nucleotides
LRK53_RS03330: LRK53_RS03330 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 785,797 to 786,621
_RS03330
LRK53_RS03335: LRK53_RS03335 - aldo/keto reductase, at 786,779 to 787,771
_RS03335
LRK53_RS03340: LRK53_RS03340 - glutathione S-transferase family protein, at 787,980 to 788,732
_RS03340
Position (kb) 786
787
788 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 785.799 kb on - strand at 785.799 kb on - strand at 785.891 kb on + strand, within LRK53_RS03330 at 785.892 kb on - strand, within LRK53_RS03330 at 786.356 kb on + strand, within LRK53_RS03330 at 786.357 kb on - strand, within LRK53_RS03330 at 786.357 kb on - strand, within LRK53_RS03330 at 786.357 kb on - strand, within LRK53_RS03330 at 786.357 kb on - strand, within LRK53_RS03330 at 786.578 kb on + strand at 786.578 kb on + strand at 786.578 kb on + strand at 786.578 kb on + strand at 786.579 kb on - strand at 786.579 kb on - strand at 786.579 kb on - strand at 786.579 kb on - strand at 786.579 kb on - strand at 786.579 kb on - strand at 786.621 kb on + strand at 786.621 kb on + strand at 786.622 kb on - strand at 786.622 kb on - strand at 786.622 kb on - strand at 786.633 kb on + strand at 786.634 kb on - strand at 786.713 kb on - strand at 786.813 kb on + strand at 786.813 kb on + strand at 786.813 kb on + strand at 786.814 kb on - strand at 787.161 kb on + strand, within LRK53_RS03335 at 787.161 kb on + strand, within LRK53_RS03335 at 787.161 kb on + strand, within LRK53_RS03335 at 787.404 kb on + strand, within LRK53_RS03335 at 787.404 kb on + strand, within LRK53_RS03335 at 787.404 kb on + strand, within LRK53_RS03335 at 787.404 kb on + strand, within LRK53_RS03335 at 787.404 kb on + strand, within LRK53_RS03335 at 787.404 kb on + strand, within LRK53_RS03335 at 787.404 kb on + strand, within LRK53_RS03335 at 787.405 kb on - strand, within LRK53_RS03335 at 787.405 kb on - strand, within LRK53_RS03335 at 787.405 kb on - strand, within LRK53_RS03335 at 787.405 kb on - strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.407 kb on + strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.408 kb on - strand, within LRK53_RS03335 at 787.467 kb on + strand, within LRK53_RS03335 at 787.467 kb on + strand, within LRK53_RS03335 at 787.467 kb on + strand, within LRK53_RS03335 at 787.467 kb on + strand, within LRK53_RS03335 at 787.468 kb on - strand, within LRK53_RS03335 at 787.468 kb on - strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.605 kb on + strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.606 kb on - strand, within LRK53_RS03335 at 787.692 kb on + strand at 787.693 kb on - strand at 787.827 kb on + strand at 787.828 kb on - strand at 788.296 kb on + strand, within LRK53_RS03340 at 788.296 kb on + strand, within LRK53_RS03340 at 788.297 kb on - strand, within LRK53_RS03340 at 788.297 kb on - strand, within LRK53_RS03340 at 788.297 kb on - strand, within LRK53_RS03340 at 788.437 kb on + strand, within LRK53_RS03340 at 788.437 kb on + strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.438 kb on - strand, within LRK53_RS03340 at 788.486 kb on - strand, within LRK53_RS03340 at 788.518 kb on + strand, within LRK53_RS03340 at 788.518 kb on + strand, within LRK53_RS03340 at 788.519 kb on - strand, within LRK53_RS03340 at 788.519 kb on - strand, within LRK53_RS03340 at 788.644 kb on + strand, within LRK53_RS03340 at 788.644 kb on + strand, within LRK53_RS03340 at 788.644 kb on + strand, within LRK53_RS03340 at 788.645 kb on - strand, within LRK53_RS03340 at 788.645 kb on - strand, within LRK53_RS03340 at 788.689 kb on + strand at 788.689 kb on + strand at 788.689 kb on + strand at 788.689 kb on + strand at 788.690 kb on - strand at 788.690 kb on - strand at 788.690 kb on - strand at 788.690 kb on - strand at 788.690 kb on - strand at 788.690 kb on - strand at 788.690 kb on - strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.701 kb on + strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand at 788.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 785,799 - -0.2 785,799 - +1.0 785,891 + LRK53_RS03330 0.11 -0.0 785,892 - LRK53_RS03330 0.12 +0.5 786,356 + LRK53_RS03330 0.68 -0.0 786,357 - LRK53_RS03330 0.68 -0.0 786,357 - LRK53_RS03330 0.68 -0.6 786,357 - LRK53_RS03330 0.68 +1.0 786,357 - LRK53_RS03330 0.68 -1.6 786,578 + -2.0 786,578 + -0.0 786,578 + +0.1 786,578 + +0.4 786,579 - -0.0 786,579 - -2.2 786,579 - -1.4 786,579 - -0.0 786,579 - -0.0 786,579 - -1.4 786,621 + -1.5 786,621 + -0.0 786,622 - -0.7 786,622 - -0.1 786,622 - -0.6 786,633 + -0.5 786,634 - -1.0 786,713 - -0.3 786,813 + -0.6 786,813 + -0.4 786,813 + -0.0 786,814 - -1.1 787,161 + LRK53_RS03335 0.38 +2.0 787,161 + LRK53_RS03335 0.38 +2.0 787,161 + LRK53_RS03335 0.38 +0.4 787,404 + LRK53_RS03335 0.63 -0.9 787,404 + LRK53_RS03335 0.63 +0.6 787,404 + LRK53_RS03335 0.63 -0.6 787,404 + LRK53_RS03335 0.63 -0.4 787,404 + LRK53_RS03335 0.63 -0.4 787,404 + LRK53_RS03335 0.63 -2.0 787,404 + LRK53_RS03335 0.63 +1.0 787,405 - LRK53_RS03335 0.63 +0.6 787,405 - LRK53_RS03335 0.63 -2.4 787,405 - LRK53_RS03335 0.63 +1.5 787,405 - LRK53_RS03335 0.63 -1.0 787,407 + LRK53_RS03335 0.63 -0.0 787,407 + LRK53_RS03335 0.63 -0.6 787,407 + LRK53_RS03335 0.63 +1.0 787,407 + LRK53_RS03335 0.63 -0.6 787,407 + LRK53_RS03335 0.63 +0.3 787,407 + LRK53_RS03335 0.63 -0.2 787,407 + LRK53_RS03335 0.63 -0.6 787,407 + LRK53_RS03335 0.63 -0.0 787,407 + LRK53_RS03335 0.63 -1.0 787,407 + LRK53_RS03335 0.63 -0.0 787,407 + LRK53_RS03335 0.63 -0.6 787,407 + LRK53_RS03335 0.63 -0.0 787,407 + LRK53_RS03335 0.63 -2.5 787,407 + LRK53_RS03335 0.63 -2.2 787,407 + LRK53_RS03335 0.63 -0.0 787,408 - LRK53_RS03335 0.63 -0.5 787,408 - LRK53_RS03335 0.63 -0.6 787,408 - LRK53_RS03335 0.63 -0.4 787,408 - LRK53_RS03335 0.63 -1.4 787,408 - LRK53_RS03335 0.63 -0.9 787,408 - LRK53_RS03335 0.63 -0.6 787,408 - LRK53_RS03335 0.63 -1.4 787,408 - LRK53_RS03335 0.63 +0.5 787,408 - LRK53_RS03335 0.63 -1.0 787,408 - LRK53_RS03335 0.63 -3.6 787,408 - LRK53_RS03335 0.63 -1.8 787,408 - LRK53_RS03335 0.63 -1.0 787,408 - LRK53_RS03335 0.63 -0.6 787,408 - LRK53_RS03335 0.63 -1.8 787,408 - LRK53_RS03335 0.63 -0.5 787,408 - LRK53_RS03335 0.63 -1.0 787,408 - LRK53_RS03335 0.63 -1.2 787,467 + LRK53_RS03335 0.69 +0.2 787,467 + LRK53_RS03335 0.69 +0.5 787,467 + LRK53_RS03335 0.69 -2.2 787,467 + LRK53_RS03335 0.69 -0.0 787,468 - LRK53_RS03335 0.69 -1.6 787,468 - LRK53_RS03335 0.69 +0.7 787,605 + LRK53_RS03335 0.83 +1.0 787,605 + LRK53_RS03335 0.83 -0.0 787,605 + LRK53_RS03335 0.83 -0.9 787,605 + LRK53_RS03335 0.83 -0.8 787,605 + LRK53_RS03335 0.83 -1.0 787,605 + LRK53_RS03335 0.83 +1.0 787,605 + LRK53_RS03335 0.83 -2.0 787,605 + LRK53_RS03335 0.83 -0.4 787,605 + LRK53_RS03335 0.83 -0.3 787,605 + LRK53_RS03335 0.83 -0.9 787,605 + LRK53_RS03335 0.83 -0.6 787,605 + LRK53_RS03335 0.83 -0.0 787,606 - LRK53_RS03335 0.83 -0.0 787,606 - LRK53_RS03335 0.83 -0.1 787,606 - LRK53_RS03335 0.83 -1.3 787,606 - LRK53_RS03335 0.83 -1.8 787,606 - LRK53_RS03335 0.83 -3.1 787,606 - LRK53_RS03335 0.83 +0.7 787,606 - LRK53_RS03335 0.83 -0.4 787,606 - LRK53_RS03335 0.83 -0.5 787,606 - LRK53_RS03335 0.83 -0.6 787,606 - LRK53_RS03335 0.83 -1.2 787,606 - LRK53_RS03335 0.83 +0.4 787,606 - LRK53_RS03335 0.83 -1.8 787,606 - LRK53_RS03335 0.83 -2.3 787,606 - LRK53_RS03335 0.83 -0.8 787,606 - LRK53_RS03335 0.83 -0.0 787,692 + -0.5 787,693 - -1.2 787,827 + +0.4 787,828 - +1.0 788,296 + LRK53_RS03340 0.42 -1.4 788,296 + LRK53_RS03340 0.42 +1.5 788,297 - LRK53_RS03340 0.42 -1.0 788,297 - LRK53_RS03340 0.42 +1.0 788,297 - LRK53_RS03340 0.42 +0.2 788,437 + LRK53_RS03340 0.61 -0.4 788,437 + LRK53_RS03340 0.61 -1.6 788,438 - LRK53_RS03340 0.61 -2.1 788,438 - LRK53_RS03340 0.61 -2.2 788,438 - LRK53_RS03340 0.61 -0.7 788,438 - LRK53_RS03340 0.61 -0.8 788,438 - LRK53_RS03340 0.61 +0.4 788,438 - LRK53_RS03340 0.61 -0.0 788,438 - LRK53_RS03340 0.61 -1.0 788,438 - LRK53_RS03340 0.61 +2.5 788,486 - LRK53_RS03340 0.67 -0.8 788,518 + LRK53_RS03340 0.71 -0.6 788,518 + LRK53_RS03340 0.71 -0.8 788,519 - LRK53_RS03340 0.72 +0.5 788,519 - LRK53_RS03340 0.72 +1.0 788,644 + LRK53_RS03340 0.88 -0.6 788,644 + LRK53_RS03340 0.88 -1.8 788,644 + LRK53_RS03340 0.88 -0.4 788,645 - LRK53_RS03340 0.88 -1.2 788,645 - LRK53_RS03340 0.88 -0.7 788,689 + -1.8 788,689 + -1.6 788,689 + -0.4 788,689 + -2.2 788,690 - +0.2 788,690 - -1.3 788,690 - -0.0 788,690 - -0.0 788,690 - +1.0 788,690 - -0.3 788,690 - -0.0 788,701 + -0.0 788,701 + +0.4 788,701 + -0.6 788,701 + -0.6 788,701 + -1.0 788,701 + -2.4 788,701 + -1.4 788,701 + -0.4 788,701 + +1.4 788,701 + -1.4 788,701 + -1.4 788,701 + -1.4 788,702 - -2.0 788,702 - -0.3 788,702 - -0.0 788,702 - +2.0 788,702 - -0.4 788,702 - -0.6 788,702 - -1.0 788,702 - -1.4 788,702 - +2.0 788,702 - -0.4 788,702 - -0.0 788,702 - -0.4
Or see this region's nucleotide sequence