Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03160 and LRK53_RS03165 overlap by 4 nucleotides LRK53_RS03165 and LRK53_RS03170 are separated by 377 nucleotides
LRK53_RS03160: LRK53_RS03160 - homoserine kinase, at 738,399 to 739,397
_RS03160
LRK53_RS03165: LRK53_RS03165 - homoserine dehydrogenase, at 739,394 to 740,545
_RS03165
LRK53_RS03170: LRK53_RS03170 - c-type cytochrome, at 740,923 to 741,699
_RS03170
Position (kb) 739
740
741 Strain fitness (log2 ratio) -2
-1
0
1
2 at 738.547 kb on + strand, within LRK53_RS03160 at 738.548 kb on - strand, within LRK53_RS03160 at 738.548 kb on - strand, within LRK53_RS03160 at 738.548 kb on - strand, within LRK53_RS03160 at 738.976 kb on + strand, within LRK53_RS03160 at 738.976 kb on + strand, within LRK53_RS03160 at 738.977 kb on - strand, within LRK53_RS03160 at 739.308 kb on + strand at 739.309 kb on - strand at 739.383 kb on + strand at 739.383 kb on + strand at 739.498 kb on + strand at 739.507 kb on - strand at 739.864 kb on - strand, within LRK53_RS03165 at 739.864 kb on - strand, within LRK53_RS03165 at 739.864 kb on - strand, within LRK53_RS03165 at 740.044 kb on - strand, within LRK53_RS03165 at 740.163 kb on + strand, within LRK53_RS03165 at 740.163 kb on + strand, within LRK53_RS03165 at 740.163 kb on + strand, within LRK53_RS03165 at 740.164 kb on - strand, within LRK53_RS03165 at 740.640 kb on + strand at 740.640 kb on + strand at 740.640 kb on + strand at 740.640 kb on + strand at 740.640 kb on + strand at 740.640 kb on + strand at 740.640 kb on + strand at 740.640 kb on + strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.641 kb on - strand at 740.679 kb on + strand at 740.679 kb on + strand at 740.679 kb on + strand at 740.679 kb on + strand at 740.679 kb on + strand at 740.680 kb on - strand at 740.680 kb on - strand at 740.680 kb on - strand at 740.680 kb on - strand at 740.680 kb on - strand at 740.680 kb on - strand at 740.680 kb on - strand at 740.691 kb on + strand at 740.691 kb on + strand at 740.691 kb on + strand at 740.691 kb on + strand at 740.691 kb on + strand at 740.691 kb on + strand at 740.692 kb on - strand at 740.692 kb on - strand at 740.692 kb on - strand at 740.692 kb on - strand at 740.692 kb on - strand at 740.692 kb on - strand at 740.692 kb on - strand at 740.692 kb on - strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.781 kb on + strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.782 kb on - strand at 740.793 kb on + strand at 740.794 kb on - strand at 740.794 kb on - strand at 740.794 kb on - strand at 740.800 kb on + strand at 740.800 kb on + strand at 740.800 kb on + strand at 740.800 kb on + strand at 740.800 kb on + strand at 740.800 kb on + strand at 740.800 kb on + strand at 740.800 kb on + strand at 740.801 kb on - strand at 740.801 kb on - strand at 740.801 kb on - strand at 740.801 kb on - strand at 740.801 kb on - strand at 740.801 kb on - strand at 740.910 kb on + strand at 740.911 kb on - strand at 740.928 kb on + strand at 740.928 kb on + strand at 740.928 kb on + strand at 740.928 kb on + strand at 740.928 kb on + strand at 740.928 kb on + strand at 740.928 kb on + strand at 740.928 kb on + strand at 740.929 kb on - strand at 740.929 kb on - strand at 740.929 kb on - strand at 740.929 kb on - strand at 740.929 kb on - strand at 740.929 kb on - strand at 740.958 kb on + strand at 740.959 kb on - strand at 740.959 kb on - strand at 740.959 kb on - strand at 741.169 kb on - strand, within LRK53_RS03170 at 741.190 kb on + strand, within LRK53_RS03170 at 741.190 kb on + strand, within LRK53_RS03170 at 741.190 kb on + strand, within LRK53_RS03170 at 741.190 kb on + strand, within LRK53_RS03170 at 741.191 kb on - strand, within LRK53_RS03170 at 741.191 kb on - strand, within LRK53_RS03170 at 741.191 kb on - strand, within LRK53_RS03170 at 741.191 kb on - strand, within LRK53_RS03170 at 741.191 kb on - strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.229 kb on + strand, within LRK53_RS03170 at 741.230 kb on - strand, within LRK53_RS03170 at 741.230 kb on - strand, within LRK53_RS03170 at 741.230 kb on - strand, within LRK53_RS03170 at 741.230 kb on - strand, within LRK53_RS03170 at 741.230 kb on - strand, within LRK53_RS03170 at 741.256 kb on + strand, within LRK53_RS03170 at 741.256 kb on + strand, within LRK53_RS03170 at 741.256 kb on + strand, within LRK53_RS03170 at 741.257 kb on - strand, within LRK53_RS03170 at 741.257 kb on - strand, within LRK53_RS03170 at 741.277 kb on + strand, within LRK53_RS03170 at 741.277 kb on + strand, within LRK53_RS03170 at 741.277 kb on + strand, within LRK53_RS03170 at 741.278 kb on - strand, within LRK53_RS03170 at 741.302 kb on - strand, within LRK53_RS03170 at 741.466 kb on + strand, within LRK53_RS03170 at 741.466 kb on + strand, within LRK53_RS03170 at 741.467 kb on - strand, within LRK53_RS03170 at 741.467 kb on - strand, within LRK53_RS03170 at 741.472 kb on + strand, within LRK53_RS03170 at 741.472 kb on + strand, within LRK53_RS03170 at 741.472 kb on + strand, within LRK53_RS03170 at 741.472 kb on + strand, within LRK53_RS03170 at 741.472 kb on + strand, within LRK53_RS03170 at 741.473 kb on - strand, within LRK53_RS03170 at 741.473 kb on - strand, within LRK53_RS03170 at 741.473 kb on - strand, within LRK53_RS03170 at 741.473 kb on - strand, within LRK53_RS03170 at 741.529 kb on + strand, within LRK53_RS03170 at 741.529 kb on + strand, within LRK53_RS03170 at 741.529 kb on + strand, within LRK53_RS03170 at 741.529 kb on + strand, within LRK53_RS03170 at 741.530 kb on - strand, within LRK53_RS03170 at 741.530 kb on - strand, within LRK53_RS03170
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 738,547 + LRK53_RS03160 0.15 -0.6 738,548 - LRK53_RS03160 0.15 -1.0 738,548 - LRK53_RS03160 0.15 -0.7 738,548 - LRK53_RS03160 0.15 -0.5 738,976 + LRK53_RS03160 0.58 +0.4 738,976 + LRK53_RS03160 0.58 -1.1 738,977 - LRK53_RS03160 0.58 +0.4 739,308 + -1.4 739,309 - -2.4 739,383 + +1.0 739,383 + +1.0 739,498 + -0.2 739,507 - +0.5 739,864 - LRK53_RS03165 0.41 -1.0 739,864 - LRK53_RS03165 0.41 -2.5 739,864 - LRK53_RS03165 0.41 -1.0 740,044 - LRK53_RS03165 0.56 -0.8 740,163 + LRK53_RS03165 0.67 -2.0 740,163 + LRK53_RS03165 0.67 -0.0 740,163 + LRK53_RS03165 0.67 -1.0 740,164 - LRK53_RS03165 0.67 -0.6 740,640 + -0.8 740,640 + -1.7 740,640 + -1.0 740,640 + -1.1 740,640 + -0.4 740,640 + +1.0 740,640 + -0.6 740,640 + -0.4 740,641 - +1.0 740,641 - -1.1 740,641 - -0.5 740,641 - +0.2 740,641 - -0.0 740,641 - -0.6 740,641 - -1.0 740,641 - +0.5 740,641 - +0.2 740,641 - -1.0 740,641 - -0.6 740,641 - -1.5 740,641 - -0.3 740,679 + -0.3 740,679 + -0.6 740,679 + +0.5 740,679 + -0.9 740,679 + +0.4 740,680 - -0.6 740,680 - -1.0 740,680 - -1.6 740,680 - +0.4 740,680 - -0.4 740,680 - -0.6 740,680 - -0.8 740,691 + -0.0 740,691 + -0.6 740,691 + -1.0 740,691 + -0.6 740,691 + -1.7 740,691 + -0.6 740,692 - +0.6 740,692 - -2.0 740,692 - +0.4 740,692 - -1.1 740,692 - -0.4 740,692 - -1.6 740,692 - -0.5 740,692 - +0.5 740,781 + -0.3 740,781 + -0.7 740,781 + -0.0 740,781 + -0.0 740,781 + +1.0 740,781 + -2.7 740,781 + -1.0 740,781 + -1.0 740,781 + +0.4 740,781 + -1.4 740,781 + +0.5 740,781 + -0.4 740,781 + -0.0 740,781 + -0.4 740,782 - +1.0 740,782 - -0.9 740,782 - -0.0 740,782 - -0.8 740,782 - -0.0 740,782 - +1.0 740,782 - -0.4 740,782 - +1.0 740,782 - -0.6 740,782 - +1.0 740,782 - -0.4 740,782 - -0.6 740,793 + +1.2 740,794 - -0.0 740,794 - -1.8 740,794 - -1.6 740,800 + -0.3 740,800 + -1.0 740,800 + -1.8 740,800 + -0.6 740,800 + -1.5 740,800 + -0.6 740,800 + -1.4 740,800 + +0.5 740,801 - -1.6 740,801 - +0.1 740,801 - -0.1 740,801 - -1.4 740,801 - -0.0 740,801 - -0.6 740,910 + -0.5 740,911 - -1.8 740,928 + -0.0 740,928 + -0.0 740,928 + -0.2 740,928 + +1.5 740,928 + -2.0 740,928 + +0.4 740,928 + +1.0 740,928 + -0.0 740,929 - -2.0 740,929 - +0.4 740,929 - -0.2 740,929 - -0.6 740,929 - -1.4 740,929 - -1.8 740,958 + -1.4 740,959 - -1.3 740,959 - +1.0 740,959 - -0.6 741,169 - LRK53_RS03170 0.32 -1.6 741,190 + LRK53_RS03170 0.34 -1.4 741,190 + LRK53_RS03170 0.34 -0.6 741,190 + LRK53_RS03170 0.34 -1.5 741,190 + LRK53_RS03170 0.34 -0.6 741,191 - LRK53_RS03170 0.34 -0.0 741,191 - LRK53_RS03170 0.34 +0.4 741,191 - LRK53_RS03170 0.34 -0.6 741,191 - LRK53_RS03170 0.34 -1.4 741,191 - LRK53_RS03170 0.34 -0.0 741,229 + LRK53_RS03170 0.39 -1.4 741,229 + LRK53_RS03170 0.39 -0.4 741,229 + LRK53_RS03170 0.39 -0.5 741,229 + LRK53_RS03170 0.39 -0.4 741,229 + LRK53_RS03170 0.39 -0.2 741,229 + LRK53_RS03170 0.39 -0.6 741,229 + LRK53_RS03170 0.39 -0.6 741,229 + LRK53_RS03170 0.39 -1.0 741,229 + LRK53_RS03170 0.39 -0.0 741,229 + LRK53_RS03170 0.39 -2.0 741,229 + LRK53_RS03170 0.39 -1.6 741,229 + LRK53_RS03170 0.39 -1.0 741,229 + LRK53_RS03170 0.39 -1.2 741,229 + LRK53_RS03170 0.39 -2.4 741,229 + LRK53_RS03170 0.39 -0.8 741,229 + LRK53_RS03170 0.39 -2.2 741,229 + LRK53_RS03170 0.39 -0.4 741,229 + LRK53_RS03170 0.39 -0.0 741,229 + LRK53_RS03170 0.39 -1.8 741,229 + LRK53_RS03170 0.39 -0.0 741,229 + LRK53_RS03170 0.39 +0.2 741,230 - LRK53_RS03170 0.40 -0.0 741,230 - LRK53_RS03170 0.40 -0.3 741,230 - LRK53_RS03170 0.40 -0.3 741,230 - LRK53_RS03170 0.40 -0.0 741,230 - LRK53_RS03170 0.40 -0.8 741,256 + LRK53_RS03170 0.43 -0.3 741,256 + LRK53_RS03170 0.43 +0.1 741,256 + LRK53_RS03170 0.43 +1.0 741,257 - LRK53_RS03170 0.43 -1.4 741,257 - LRK53_RS03170 0.43 -1.0 741,277 + LRK53_RS03170 0.46 -0.8 741,277 + LRK53_RS03170 0.46 -0.0 741,277 + LRK53_RS03170 0.46 -1.0 741,278 - LRK53_RS03170 0.46 -1.6 741,302 - LRK53_RS03170 0.49 -1.1 741,466 + LRK53_RS03170 0.70 -1.4 741,466 + LRK53_RS03170 0.70 +1.0 741,467 - LRK53_RS03170 0.70 -0.0 741,467 - LRK53_RS03170 0.70 -1.2 741,472 + LRK53_RS03170 0.71 -0.4 741,472 + LRK53_RS03170 0.71 +1.0 741,472 + LRK53_RS03170 0.71 -0.4 741,472 + LRK53_RS03170 0.71 -0.0 741,472 + LRK53_RS03170 0.71 +1.5 741,473 - LRK53_RS03170 0.71 -0.3 741,473 - LRK53_RS03170 0.71 -0.4 741,473 - LRK53_RS03170 0.71 +2.0 741,473 - LRK53_RS03170 0.71 +0.5 741,529 + LRK53_RS03170 0.78 -0.1 741,529 + LRK53_RS03170 0.78 -0.0 741,529 + LRK53_RS03170 0.78 -0.0 741,529 + LRK53_RS03170 0.78 -1.4 741,530 - LRK53_RS03170 0.78 +1.0 741,530 - LRK53_RS03170 0.78 -0.6
Or see this region's nucleotide sequence