Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02395 and LRK53_RS02400 are separated by 222 nucleotides LRK53_RS02400 and LRK53_RS02405 are separated by 5 nucleotides LRK53_RS02405 and LRK53_RS02410 are separated by 178 nucleotides
LRK53_RS02395: LRK53_RS02395 - cold-shock protein, at 550,378 to 550,587
_RS02395
LRK53_RS02400: LRK53_RS02400 - exodeoxyribonuclease III, at 550,810 to 551,577
_RS02400
LRK53_RS02405: LRK53_RS02405 - YecA family protein, at 551,583 to 552,260
_RS02405
LRK53_RS02410: LRK53_RS02410 - pirin family protein, at 552,439 to 553,272
_RS02410
Position (kb) 551
552
553 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 550.798 kb on + strand at 550.799 kb on - strand at 550.799 kb on - strand at 551.155 kb on + strand, within LRK53_RS02400 at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.572 kb on - strand at 551.572 kb on - strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.647 kb on - strand at 551.863 kb on - strand, within LRK53_RS02405 at 552.090 kb on + strand, within LRK53_RS02405 at 552.091 kb on - strand, within LRK53_RS02405 at 552.091 kb on - strand, within LRK53_RS02405 at 552.091 kb on - strand, within LRK53_RS02405 at 552.141 kb on + strand, within LRK53_RS02405 at 552.141 kb on + strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.386 kb on + strand at 552.386 kb on + strand at 552.387 kb on - strand at 552.387 kb on - strand at 552.387 kb on - strand at 552.387 kb on - strand at 552.592 kb on + strand, within LRK53_RS02410 at 552.592 kb on + strand, within LRK53_RS02410 at 552.592 kb on + strand, within LRK53_RS02410 at 552.592 kb on + strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.646 kb on - strand, within LRK53_RS02410 at 552.838 kb on + strand, within LRK53_RS02410 at 552.964 kb on + strand, within LRK53_RS02410 at 552.964 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 550,798 + -1.0 550,799 - -0.4 550,799 - -0.6 551,155 + LRK53_RS02400 0.45 -1.0 551,571 + +0.4 551,571 + +0.4 551,571 + +0.4 551,571 + -0.4 551,571 + +1.0 551,571 + -1.5 551,571 + -0.0 551,571 + -1.7 551,572 - +1.0 551,572 - -2.0 551,646 + +0.4 551,646 + -1.4 551,646 + -0.8 551,646 + -0.6 551,646 + +1.5 551,647 - -0.0 551,863 - LRK53_RS02405 0.41 -0.6 552,090 + LRK53_RS02405 0.75 -1.2 552,091 - LRK53_RS02405 0.75 +1.0 552,091 - LRK53_RS02405 0.75 -3.4 552,091 - LRK53_RS02405 0.75 +1.0 552,141 + LRK53_RS02405 0.82 -0.6 552,141 + LRK53_RS02405 0.82 -1.4 552,142 - LRK53_RS02405 0.82 -0.6 552,142 - LRK53_RS02405 0.82 -0.6 552,142 - LRK53_RS02405 0.82 -0.4 552,142 - LRK53_RS02405 0.82 -1.4 552,280 + -0.4 552,280 + -0.6 552,280 + -0.6 552,280 + -1.0 552,280 + +0.2 552,280 + -1.0 552,280 + -1.0 552,280 + -0.6 552,280 + +1.0 552,281 - -0.7 552,281 - +1.0 552,281 - -0.8 552,281 - -0.4 552,281 - -1.6 552,281 - -0.6 552,281 - +2.5 552,281 - +1.4 552,281 - +0.1 552,281 - +0.7 552,386 + -1.4 552,386 + -1.0 552,387 - +0.5 552,387 - +1.4 552,387 - -1.6 552,387 - -0.4 552,592 + LRK53_RS02410 0.18 -0.9 552,592 + LRK53_RS02410 0.18 -1.4 552,592 + LRK53_RS02410 0.18 -0.6 552,592 + LRK53_RS02410 0.18 +1.0 552,593 - LRK53_RS02410 0.18 -0.6 552,593 - LRK53_RS02410 0.18 -0.6 552,593 - LRK53_RS02410 0.18 -0.6 552,593 - LRK53_RS02410 0.18 -0.7 552,593 - LRK53_RS02410 0.18 -1.0 552,593 - LRK53_RS02410 0.18 -0.0 552,593 - LRK53_RS02410 0.18 -0.6 552,593 - LRK53_RS02410 0.18 -1.4 552,643 + LRK53_RS02410 0.24 +1.5 552,643 + LRK53_RS02410 0.24 -1.6 552,643 + LRK53_RS02410 0.24 +0.6 552,643 + LRK53_RS02410 0.24 -1.8 552,643 + LRK53_RS02410 0.24 -1.4 552,643 + LRK53_RS02410 0.24 +0.4 552,643 + LRK53_RS02410 0.24 -0.0 552,643 + LRK53_RS02410 0.24 -0.7 552,643 + LRK53_RS02410 0.24 -1.6 552,643 + LRK53_RS02410 0.24 -1.7 552,643 + LRK53_RS02410 0.24 -1.4 552,643 + LRK53_RS02410 0.24 -1.4 552,643 + LRK53_RS02410 0.24 +1.0 552,643 + LRK53_RS02410 0.24 -0.7 552,643 + LRK53_RS02410 0.24 -0.6 552,643 + LRK53_RS02410 0.24 -1.0 552,643 + LRK53_RS02410 0.24 -0.6 552,643 + LRK53_RS02410 0.24 -0.9 552,643 + LRK53_RS02410 0.24 -1.6 552,643 + LRK53_RS02410 0.24 -0.6 552,643 + LRK53_RS02410 0.24 -0.0 552,643 + LRK53_RS02410 0.24 -0.6 552,643 + LRK53_RS02410 0.24 -0.4 552,643 + LRK53_RS02410 0.24 +0.5 552,643 + LRK53_RS02410 0.24 -2.0 552,643 + LRK53_RS02410 0.24 -1.2 552,643 + LRK53_RS02410 0.24 -0.6 552,643 + LRK53_RS02410 0.24 -1.0 552,643 + LRK53_RS02410 0.24 -1.0 552,643 + LRK53_RS02410 0.24 -0.6 552,643 + LRK53_RS02410 0.24 -0.0 552,643 + LRK53_RS02410 0.24 -0.3 552,643 + LRK53_RS02410 0.24 -0.3 552,644 - LRK53_RS02410 0.25 -0.6 552,644 - LRK53_RS02410 0.25 -1.0 552,644 - LRK53_RS02410 0.25 -2.4 552,644 - LRK53_RS02410 0.25 -0.5 552,644 - LRK53_RS02410 0.25 -0.0 552,644 - LRK53_RS02410 0.25 +0.2 552,644 - LRK53_RS02410 0.25 +0.4 552,644 - LRK53_RS02410 0.25 +0.2 552,644 - LRK53_RS02410 0.25 -0.5 552,644 - LRK53_RS02410 0.25 +0.2 552,644 - LRK53_RS02410 0.25 -0.9 552,644 - LRK53_RS02410 0.25 -0.9 552,644 - LRK53_RS02410 0.25 -0.5 552,644 - LRK53_RS02410 0.25 -1.6 552,644 - LRK53_RS02410 0.25 -1.2 552,644 - LRK53_RS02410 0.25 -1.0 552,644 - LRK53_RS02410 0.25 +0.4 552,644 - LRK53_RS02410 0.25 -1.8 552,644 - LRK53_RS02410 0.25 -0.0 552,644 - LRK53_RS02410 0.25 -0.9 552,644 - LRK53_RS02410 0.25 -1.1 552,644 - LRK53_RS02410 0.25 -0.3 552,644 - LRK53_RS02410 0.25 -0.0 552,644 - LRK53_RS02410 0.25 -0.5 552,646 - LRK53_RS02410 0.25 -0.0 552,838 + LRK53_RS02410 0.48 +2.3 552,964 + LRK53_RS02410 0.63 -0.0 552,964 + LRK53_RS02410 0.63 -0.0 552,997 + LRK53_RS02410 0.67 -1.4 552,997 + LRK53_RS02410 0.67 -0.6 552,997 + LRK53_RS02410 0.67 -1.0 552,997 + LRK53_RS02410 0.67 -0.5 552,997 + LRK53_RS02410 0.67 -4.0 552,997 + LRK53_RS02410 0.67 -1.8 552,997 + LRK53_RS02410 0.67 -0.0 552,997 + LRK53_RS02410 0.67 +0.8 552,998 - LRK53_RS02410 0.67 -0.0 552,998 - LRK53_RS02410 0.67 -0.0 552,998 - LRK53_RS02410 0.67 -0.6 552,998 - LRK53_RS02410 0.67 -2.9 552,998 - LRK53_RS02410 0.67 -0.4 553,037 - LRK53_RS02410 0.72 -0.3 553,037 - LRK53_RS02410 0.72 +1.0 553,037 - LRK53_RS02410 0.72 -0.0 553,037 - LRK53_RS02410 0.72 +0.5 553,037 - LRK53_RS02410 0.72 +0.2 553,037 - LRK53_RS02410 0.72 -2.8
Or see this region's nucleotide sequence