Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02260 and LRK53_RS02265 overlap by 4 nucleotides LRK53_RS02265 and LRK53_RS02270 overlap by 4 nucleotides LRK53_RS02270 and LRK53_RS02275 are separated by 103 nucleotides LRK53_RS02275 and LRK53_RS02280 are separated by 68 nucleotides
LRK53_RS02260: LRK53_RS02260 - glycosyltransferase family 2 protein, at 523,980 to 524,765
_RS02260
LRK53_RS02265: LRK53_RS02265 - beta-ketoacyl synthase chain length factor, at 524,762 to 525,556
_RS02265
LRK53_RS02270: LRK53_RS02270 - beta-ketoacyl-[acyl-carrier-protein] synthase family protein, at 525,553 to 526,746
_RS02270
LRK53_RS02275: LRK53_RS02275 - YdcF family protein, at 526,850 to 527,587
_RS02275
LRK53_RS02280: LRK53_RS02280 - hypothetical protein, at 527,656 to 528,168
_RS02280
Position (kb)
525
526
527 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 524.785 kb on + strand at 524.786 kb on - strand at 524.786 kb on - strand at 524.786 kb on - strand at 524.800 kb on + strand at 524.800 kb on + strand at 524.801 kb on - strand at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.276 kb on + strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.277 kb on - strand, within LRK53_RS02265 at 525.539 kb on + strand at 525.540 kb on - strand at 525.893 kb on + strand, within LRK53_RS02270 at 525.893 kb on + strand, within LRK53_RS02270 at 525.893 kb on + strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.894 kb on - strand, within LRK53_RS02270 at 525.989 kb on + strand, within LRK53_RS02270 at 525.989 kb on + strand, within LRK53_RS02270 at 525.989 kb on + strand, within LRK53_RS02270 at 525.990 kb on - strand, within LRK53_RS02270 at 526.721 kb on + strand at 526.721 kb on + strand at 526.721 kb on + strand at 526.721 kb on + strand at 526.722 kb on - strand at 526.722 kb on - strand at 526.722 kb on - strand at 526.792 kb on + strand at 526.792 kb on + strand at 526.792 kb on + strand at 526.792 kb on + strand at 526.792 kb on + strand at 526.792 kb on + strand at 526.792 kb on + strand at 526.793 kb on - strand at 526.793 kb on - strand at 526.793 kb on - strand at 526.793 kb on - strand at 526.793 kb on - strand at 526.793 kb on - strand at 526.793 kb on - strand at 526.849 kb on + strand at 526.849 kb on + strand at 526.849 kb on + strand at 526.849 kb on + strand at 526.850 kb on - strand at 526.850 kb on - strand at 526.922 kb on + strand at 526.923 kb on - strand at 526.923 kb on - strand at 526.923 kb on - strand at 526.946 kb on + strand, within LRK53_RS02275 at 526.946 kb on + strand, within LRK53_RS02275 at 526.946 kb on + strand, within LRK53_RS02275 at 526.946 kb on + strand, within LRK53_RS02275 at 526.946 kb on + strand, within LRK53_RS02275 at 526.947 kb on - strand, within LRK53_RS02275 at 526.947 kb on - strand, within LRK53_RS02275 at 526.947 kb on - strand, within LRK53_RS02275 at 526.947 kb on - strand, within LRK53_RS02275 at 526.947 kb on - strand, within LRK53_RS02275 at 527.006 kb on + strand, within LRK53_RS02275 at 527.006 kb on + strand, within LRK53_RS02275 at 527.007 kb on - strand, within LRK53_RS02275 at 527.007 kb on - strand, within LRK53_RS02275 at 527.007 kb on - strand, within LRK53_RS02275 at 527.007 kb on - strand, within LRK53_RS02275 at 527.117 kb on + strand, within LRK53_RS02275 at 527.118 kb on - strand, within LRK53_RS02275 at 527.481 kb on - strand, within LRK53_RS02275 at 527.481 kb on - strand, within LRK53_RS02275 at 527.481 kb on - strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.510 kb on + strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275 at 527.511 kb on - strand, within LRK53_RS02275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 524,785 + -0.6 524,786 - -0.6 524,786 - -0.0 524,786 - +2.0 524,800 + +0.5 524,800 + -1.7 524,801 - -1.4 525,276 + LRK53_RS02265 0.65 +1.0 525,276 + LRK53_RS02265 0.65 +0.1 525,276 + LRK53_RS02265 0.65 -1.4 525,276 + LRK53_RS02265 0.65 -1.4 525,276 + LRK53_RS02265 0.65 +0.6 525,276 + LRK53_RS02265 0.65 +1.0 525,276 + LRK53_RS02265 0.65 +0.7 525,276 + LRK53_RS02265 0.65 -0.0 525,276 + LRK53_RS02265 0.65 -0.8 525,276 + LRK53_RS02265 0.65 -1.0 525,276 + LRK53_RS02265 0.65 -0.4 525,276 + LRK53_RS02265 0.65 +0.1 525,276 + LRK53_RS02265 0.65 -0.0 525,276 + LRK53_RS02265 0.65 -1.0 525,276 + LRK53_RS02265 0.65 -1.0 525,276 + LRK53_RS02265 0.65 -0.0 525,277 - LRK53_RS02265 0.65 +1.0 525,277 - LRK53_RS02265 0.65 -0.0 525,277 - LRK53_RS02265 0.65 +0.2 525,277 - LRK53_RS02265 0.65 -1.4 525,277 - LRK53_RS02265 0.65 -1.6 525,277 - LRK53_RS02265 0.65 +2.5 525,277 - LRK53_RS02265 0.65 +2.0 525,277 - LRK53_RS02265 0.65 -0.6 525,277 - LRK53_RS02265 0.65 -2.3 525,277 - LRK53_RS02265 0.65 -1.4 525,277 - LRK53_RS02265 0.65 -0.6 525,277 - LRK53_RS02265 0.65 +0.4 525,277 - LRK53_RS02265 0.65 -0.6 525,277 - LRK53_RS02265 0.65 -0.0 525,277 - LRK53_RS02265 0.65 -0.6 525,277 - LRK53_RS02265 0.65 -0.5 525,277 - LRK53_RS02265 0.65 +1.0 525,277 - LRK53_RS02265 0.65 +0.5 525,277 - LRK53_RS02265 0.65 -2.2 525,277 - LRK53_RS02265 0.65 -0.0 525,277 - LRK53_RS02265 0.65 -1.0 525,277 - LRK53_RS02265 0.65 -0.6 525,277 - LRK53_RS02265 0.65 +0.4 525,277 - LRK53_RS02265 0.65 -0.0 525,277 - LRK53_RS02265 0.65 -1.0 525,277 - LRK53_RS02265 0.65 -2.6 525,539 + -1.2 525,540 - +2.0 525,893 + LRK53_RS02270 0.28 -1.2 525,893 + LRK53_RS02270 0.28 -0.0 525,893 + LRK53_RS02270 0.28 -1.5 525,894 - LRK53_RS02270 0.29 -0.7 525,894 - LRK53_RS02270 0.29 -1.3 525,894 - LRK53_RS02270 0.29 -2.2 525,894 - LRK53_RS02270 0.29 -1.3 525,894 - LRK53_RS02270 0.29 -2.2 525,894 - LRK53_RS02270 0.29 -1.0 525,894 - LRK53_RS02270 0.29 -0.2 525,894 - LRK53_RS02270 0.29 -1.2 525,894 - LRK53_RS02270 0.29 -2.2 525,894 - LRK53_RS02270 0.29 -1.5 525,989 + LRK53_RS02270 0.37 -1.0 525,989 + LRK53_RS02270 0.37 -1.2 525,989 + LRK53_RS02270 0.37 -0.0 525,990 - LRK53_RS02270 0.37 -0.0 526,721 + -0.4 526,721 + -2.0 526,721 + -0.6 526,721 + -1.1 526,722 - -0.6 526,722 - -1.8 526,722 - -0.2 526,792 + -1.0 526,792 + -1.6 526,792 + +1.0 526,792 + -0.9 526,792 + -0.0 526,792 + +0.4 526,792 + +1.0 526,793 - +1.0 526,793 - -1.4 526,793 - +0.4 526,793 - -1.6 526,793 - +0.4 526,793 - -0.6 526,793 - -0.6 526,849 + +0.7 526,849 + -0.6 526,849 + -0.0 526,849 + -0.9 526,850 - -1.6 526,850 - +1.5 526,922 + -0.0 526,923 - -2.8 526,923 - -1.4 526,923 - -1.1 526,946 + LRK53_RS02275 0.13 -0.0 526,946 + LRK53_RS02275 0.13 +0.5 526,946 + LRK53_RS02275 0.13 -0.8 526,946 + LRK53_RS02275 0.13 -0.8 526,946 + LRK53_RS02275 0.13 -2.0 526,947 - LRK53_RS02275 0.13 -0.3 526,947 - LRK53_RS02275 0.13 -0.0 526,947 - LRK53_RS02275 0.13 -0.0 526,947 - LRK53_RS02275 0.13 +0.5 526,947 - LRK53_RS02275 0.13 -0.0 527,006 + LRK53_RS02275 0.21 +1.3 527,006 + LRK53_RS02275 0.21 +0.4 527,007 - LRK53_RS02275 0.21 -1.0 527,007 - LRK53_RS02275 0.21 -0.0 527,007 - LRK53_RS02275 0.21 +1.0 527,007 - LRK53_RS02275 0.21 -1.2 527,117 + LRK53_RS02275 0.36 +2.0 527,118 - LRK53_RS02275 0.36 -0.2 527,481 - LRK53_RS02275 0.86 -0.0 527,481 - LRK53_RS02275 0.86 -0.0 527,481 - LRK53_RS02275 0.86 +1.0 527,510 + LRK53_RS02275 0.89 -0.7 527,510 + LRK53_RS02275 0.89 -0.4 527,510 + LRK53_RS02275 0.89 -0.3 527,510 + LRK53_RS02275 0.89 -1.0 527,510 + LRK53_RS02275 0.89 -0.8 527,510 + LRK53_RS02275 0.89 +2.0 527,511 - LRK53_RS02275 0.90 +0.2 527,511 - LRK53_RS02275 0.90 +2.5 527,511 - LRK53_RS02275 0.90 -1.5 527,511 - LRK53_RS02275 0.90 -1.0
Or see this region's nucleotide sequence