Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02125 and LRK53_RS02130 overlap by 4 nucleotides LRK53_RS02130 and LRK53_RS02135 overlap by 4 nucleotides LRK53_RS02135 and LRK53_RS02140 are separated by 2 nucleotides LRK53_RS02140 and LRK53_RS02145 are separated by 5 nucleotides LRK53_RS02145 and LRK53_RS02150 are separated by 608 nucleotides
LRK53_RS02125: LRK53_RS02125 - DUF3987 domain-containing protein, at 502,132 to 503,655
_RS02125
LRK53_RS02130: LRK53_RS02130 - hypothetical protein, at 503,652 to 503,822
_RS02130
LRK53_RS02135: LRK53_RS02135 - hypothetical protein, at 503,819 to 504,109
_RS02135
LRK53_RS02140: LRK53_RS02140 - hypothetical protein, at 504,112 to 504,528
_RS02140
LRK53_RS02145: LRK53_RS02145 - hypothetical protein, at 504,534 to 504,866
_RS02145
LRK53_RS02150: LRK53_RS02150 - 6-carboxytetrahydropterin synthase QueD, at 505,475 to 505,837
_RS02150
Position (kb) 504
505 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 503.202 kb on + strand, within LRK53_RS02125 at 503.203 kb on - strand, within LRK53_RS02125 at 503.203 kb on - strand, within LRK53_RS02125 at 503.203 kb on - strand, within LRK53_RS02125 at 503.277 kb on + strand, within LRK53_RS02125 at 503.277 kb on + strand, within LRK53_RS02125 at 503.277 kb on + strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.278 kb on - strand, within LRK53_RS02125 at 503.295 kb on - strand, within LRK53_RS02125 at 503.386 kb on + strand, within LRK53_RS02125 at 503.414 kb on + strand, within LRK53_RS02125 at 503.414 kb on + strand, within LRK53_RS02125 at 503.414 kb on + strand, within LRK53_RS02125 at 503.414 kb on + strand, within LRK53_RS02125 at 503.415 kb on - strand, within LRK53_RS02125 at 503.429 kb on - strand, within LRK53_RS02125 at 503.429 kb on - strand, within LRK53_RS02125 at 503.429 kb on - strand, within LRK53_RS02125 at 503.470 kb on + strand, within LRK53_RS02125 at 503.470 kb on + strand, within LRK53_RS02125 at 503.470 kb on + strand, within LRK53_RS02125 at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.503 kb on + strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.504 kb on - strand at 503.507 kb on + strand at 503.507 kb on + strand at 503.507 kb on + strand at 503.508 kb on - strand at 503.508 kb on - strand at 503.508 kb on - strand at 503.508 kb on - strand at 503.508 kb on - strand at 503.508 kb on - strand at 503.539 kb on + strand at 503.540 kb on - strand at 503.588 kb on + strand at 503.588 kb on + strand at 503.589 kb on - strand at 503.790 kb on - strand, within LRK53_RS02130 at 503.790 kb on - strand, within LRK53_RS02130 at 503.866 kb on + strand, within LRK53_RS02135 at 503.867 kb on - strand, within LRK53_RS02135 at 503.944 kb on + strand, within LRK53_RS02135 at 503.944 kb on + strand, within LRK53_RS02135 at 503.944 kb on + strand, within LRK53_RS02135 at 503.944 kb on + strand, within LRK53_RS02135 at 503.944 kb on + strand, within LRK53_RS02135 at 503.945 kb on - strand, within LRK53_RS02135 at 504.103 kb on + strand at 504.103 kb on + strand at 504.103 kb on + strand at 504.103 kb on + strand at 504.104 kb on - strand at 504.104 kb on - strand at 504.104 kb on - strand at 504.104 kb on - strand at 504.104 kb on - strand at 504.338 kb on + strand, within LRK53_RS02140 at 504.338 kb on + strand, within LRK53_RS02140 at 504.338 kb on + strand, within LRK53_RS02140 at 504.339 kb on - strand, within LRK53_RS02140 at 504.339 kb on - strand, within LRK53_RS02140 at 504.339 kb on - strand, within LRK53_RS02140 at 504.339 kb on - strand, within LRK53_RS02140 at 504.357 kb on - strand, within LRK53_RS02140 at 504.444 kb on + strand, within LRK53_RS02140 at 504.444 kb on + strand, within LRK53_RS02140 at 504.444 kb on + strand, within LRK53_RS02140 at 504.444 kb on + strand, within LRK53_RS02140 at 504.445 kb on - strand, within LRK53_RS02140 at 504.445 kb on - strand, within LRK53_RS02140 at 504.456 kb on + strand, within LRK53_RS02140 at 504.471 kb on + strand, within LRK53_RS02140 at 504.471 kb on + strand, within LRK53_RS02140 at 504.471 kb on + strand, within LRK53_RS02140 at 504.472 kb on - strand, within LRK53_RS02140 at 504.535 kb on + strand at 504.559 kb on + strand at 504.560 kb on - strand at 504.560 kb on - strand at 504.560 kb on - strand at 504.691 kb on + strand, within LRK53_RS02145 at 504.737 kb on + strand, within LRK53_RS02145 at 504.737 kb on + strand, within LRK53_RS02145 at 504.737 kb on + strand, within LRK53_RS02145 at 504.737 kb on + strand, within LRK53_RS02145 at 504.738 kb on - strand, within LRK53_RS02145 at 504.738 kb on - strand, within LRK53_RS02145 at 504.738 kb on - strand, within LRK53_RS02145 at 504.808 kb on + strand, within LRK53_RS02145 at 504.808 kb on + strand, within LRK53_RS02145 at 504.808 kb on + strand, within LRK53_RS02145 at 504.809 kb on - strand, within LRK53_RS02145 at 504.809 kb on - strand, within LRK53_RS02145 at 504.813 kb on + strand, within LRK53_RS02145 at 504.813 kb on + strand, within LRK53_RS02145 at 504.813 kb on + strand, within LRK53_RS02145 at 504.813 kb on + strand, within LRK53_RS02145 at 504.814 kb on - strand, within LRK53_RS02145 at 504.814 kb on - strand, within LRK53_RS02145 at 504.847 kb on - strand at 504.885 kb on + strand at 504.885 kb on + strand at 504.885 kb on + strand at 504.885 kb on + strand at 504.885 kb on + strand at 504.885 kb on + strand at 504.885 kb on + strand at 504.886 kb on - strand at 504.886 kb on - strand at 504.886 kb on - strand at 504.886 kb on - strand at 504.886 kb on - strand at 504.907 kb on + strand at 504.907 kb on + strand at 504.907 kb on + strand at 504.908 kb on - strand at 504.908 kb on - strand at 504.908 kb on - strand at 504.908 kb on - strand at 504.908 kb on - strand at 504.908 kb on - strand at 504.964 kb on - strand at 505.041 kb on + strand at 505.041 kb on + strand at 505.041 kb on + strand at 505.051 kb on + strand at 505.052 kb on - strand at 505.107 kb on + strand at 505.107 kb on + strand at 505.107 kb on + strand at 505.108 kb on - strand at 505.111 kb on + strand at 505.112 kb on - strand at 505.112 kb on - strand at 505.152 kb on + strand at 505.152 kb on + strand at 505.152 kb on + strand at 505.152 kb on + strand at 505.152 kb on + strand at 505.152 kb on + strand at 505.153 kb on - strand at 505.153 kb on - strand at 505.153 kb on - strand at 505.153 kb on - strand at 505.153 kb on - strand at 505.153 kb on - strand at 505.153 kb on - strand at 505.188 kb on + strand at 505.188 kb on + strand at 505.188 kb on + strand at 505.188 kb on + strand at 505.189 kb on - strand at 505.189 kb on - strand at 505.189 kb on - strand at 505.189 kb on - strand at 505.210 kb on + strand at 505.210 kb on + strand at 505.210 kb on + strand at 505.210 kb on + strand at 505.211 kb on - strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.230 kb on + strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.231 kb on - strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.232 kb on + strand at 505.233 kb on - strand at 505.233 kb on - strand at 505.233 kb on - strand at 505.233 kb on - strand at 505.233 kb on - strand at 505.233 kb on - strand at 505.233 kb on - strand at 505.233 kb on - strand at 505.244 kb on + strand at 505.244 kb on + strand at 505.244 kb on + strand at 505.244 kb on + strand at 505.244 kb on + strand at 505.281 kb on - strand at 505.285 kb on + strand at 505.285 kb on + strand at 505.285 kb on + strand at 505.285 kb on + strand at 505.285 kb on + strand at 505.286 kb on - strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.288 kb on + strand at 505.289 kb on - strand at 505.289 kb on - strand at 505.289 kb on - strand at 505.289 kb on - strand at 505.289 kb on - strand at 505.289 kb on - strand at 505.289 kb on - strand at 505.391 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.480 kb on + strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand at 505.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 503,202 + LRK53_RS02125 0.70 -0.0 503,203 - LRK53_RS02125 0.70 -0.2 503,203 - LRK53_RS02125 0.70 -0.6 503,203 - LRK53_RS02125 0.70 -1.8 503,277 + LRK53_RS02125 0.75 +0.4 503,277 + LRK53_RS02125 0.75 +1.5 503,277 + LRK53_RS02125 0.75 +1.0 503,278 - LRK53_RS02125 0.75 -0.6 503,278 - LRK53_RS02125 0.75 +1.0 503,278 - LRK53_RS02125 0.75 -0.0 503,278 - LRK53_RS02125 0.75 -1.8 503,278 - LRK53_RS02125 0.75 -1.3 503,278 - LRK53_RS02125 0.75 -0.0 503,278 - LRK53_RS02125 0.75 +1.0 503,278 - LRK53_RS02125 0.75 -0.0 503,295 - LRK53_RS02125 0.76 -0.8 503,386 + LRK53_RS02125 0.82 -0.2 503,414 + LRK53_RS02125 0.84 -0.0 503,414 + LRK53_RS02125 0.84 -0.3 503,414 + LRK53_RS02125 0.84 -0.0 503,414 + LRK53_RS02125 0.84 -1.5 503,415 - LRK53_RS02125 0.84 +0.4 503,429 - LRK53_RS02125 0.85 -1.2 503,429 - LRK53_RS02125 0.85 -0.9 503,429 - LRK53_RS02125 0.85 -0.5 503,470 + LRK53_RS02125 0.88 -0.8 503,470 + LRK53_RS02125 0.88 +0.4 503,470 + LRK53_RS02125 0.88 +3.0 503,503 + -1.7 503,503 + -0.9 503,503 + -1.1 503,503 + +1.0 503,503 + -1.2 503,503 + -0.0 503,503 + -0.5 503,503 + -3.4 503,503 + -0.6 503,503 + -0.0 503,503 + -0.3 503,503 + -1.2 503,503 + -0.6 503,503 + -1.8 503,503 + -0.9 503,503 + +0.5 503,503 + -1.0 503,503 + -0.6 503,503 + -0.4 503,504 - -0.0 503,504 - -2.0 503,504 - +0.6 503,504 - -1.2 503,504 - -0.4 503,504 - -0.3 503,504 - -0.9 503,504 - +0.0 503,504 - +0.4 503,504 - +2.5 503,504 - -1.1 503,504 - +0.2 503,504 - -2.0 503,504 - +1.0 503,504 - -1.9 503,504 - +0.5 503,504 - -1.2 503,504 - -1.0 503,507 + -0.2 503,507 + +0.5 503,507 + +2.0 503,508 - -0.0 503,508 - -0.2 503,508 - -1.6 503,508 - -0.0 503,508 - -0.0 503,508 - -1.4 503,539 + -0.5 503,540 - -0.0 503,588 + -0.0 503,588 + -0.9 503,589 - -1.0 503,790 - LRK53_RS02130 0.81 -0.0 503,790 - LRK53_RS02130 0.81 -1.0 503,866 + LRK53_RS02135 0.16 -0.8 503,867 - LRK53_RS02135 0.16 -2.0 503,944 + LRK53_RS02135 0.43 -1.4 503,944 + LRK53_RS02135 0.43 +0.4 503,944 + LRK53_RS02135 0.43 -0.8 503,944 + LRK53_RS02135 0.43 +0.4 503,944 + LRK53_RS02135 0.43 +0.5 503,945 - LRK53_RS02135 0.43 -2.4 504,103 + +0.6 504,103 + +1.4 504,103 + -1.4 504,103 + -0.8 504,104 - +1.0 504,104 - -1.8 504,104 - -0.6 504,104 - +3.0 504,104 - -0.6 504,338 + LRK53_RS02140 0.54 -0.0 504,338 + LRK53_RS02140 0.54 -1.3 504,338 + LRK53_RS02140 0.54 -0.6 504,339 - LRK53_RS02140 0.54 +0.2 504,339 - LRK53_RS02140 0.54 -0.9 504,339 - LRK53_RS02140 0.54 -0.6 504,339 - LRK53_RS02140 0.54 -1.4 504,357 - LRK53_RS02140 0.59 -2.4 504,444 + LRK53_RS02140 0.80 -0.2 504,444 + LRK53_RS02140 0.80 -0.6 504,444 + LRK53_RS02140 0.80 -1.2 504,444 + LRK53_RS02140 0.80 -0.7 504,445 - LRK53_RS02140 0.80 -0.9 504,445 - LRK53_RS02140 0.80 -1.0 504,456 + LRK53_RS02140 0.82 -2.7 504,471 + LRK53_RS02140 0.86 -1.4 504,471 + LRK53_RS02140 0.86 -1.0 504,471 + LRK53_RS02140 0.86 -0.0 504,472 - LRK53_RS02140 0.86 -1.4 504,535 + -0.0 504,559 + -0.3 504,560 - -0.1 504,560 - -1.0 504,560 - +0.7 504,691 + LRK53_RS02145 0.47 -0.0 504,737 + LRK53_RS02145 0.61 -0.4 504,737 + LRK53_RS02145 0.61 -0.3 504,737 + LRK53_RS02145 0.61 -0.9 504,737 + LRK53_RS02145 0.61 +0.5 504,738 - LRK53_RS02145 0.61 -0.6 504,738 - LRK53_RS02145 0.61 -2.9 504,738 - LRK53_RS02145 0.61 -0.9 504,808 + LRK53_RS02145 0.82 -0.2 504,808 + LRK53_RS02145 0.82 -1.6 504,808 + LRK53_RS02145 0.82 -0.2 504,809 - LRK53_RS02145 0.83 -1.0 504,809 - LRK53_RS02145 0.83 -0.6 504,813 + LRK53_RS02145 0.84 -1.0 504,813 + LRK53_RS02145 0.84 +2.0 504,813 + LRK53_RS02145 0.84 -1.6 504,813 + LRK53_RS02145 0.84 -0.8 504,814 - LRK53_RS02145 0.84 -0.4 504,814 - LRK53_RS02145 0.84 -0.9 504,847 - +0.2 504,885 + -0.4 504,885 + -1.0 504,885 + -0.8 504,885 + -2.9 504,885 + -2.5 504,885 + -2.0 504,885 + +1.0 504,886 - +2.0 504,886 - -1.4 504,886 - -0.6 504,886 - -1.7 504,886 - -1.0 504,907 + -0.6 504,907 + -0.0 504,907 + -0.6 504,908 - -0.8 504,908 - +1.0 504,908 - -1.5 504,908 - -1.0 504,908 - +2.1 504,908 - -1.8 504,964 - -0.6 505,041 + -1.5 505,041 + +1.0 505,041 + -0.3 505,051 + -0.0 505,052 - +0.4 505,107 + -1.0 505,107 + -0.8 505,107 + -0.3 505,108 - +0.6 505,111 + -0.1 505,112 - -0.9 505,112 - -0.6 505,152 + -1.5 505,152 + +1.0 505,152 + -1.3 505,152 + +1.5 505,152 + -0.2 505,152 + -0.4 505,153 - -0.6 505,153 - -2.2 505,153 - +3.0 505,153 - +2.5 505,153 - -0.6 505,153 - +1.0 505,153 - +0.5 505,188 + +1.0 505,188 + -1.8 505,188 + -0.8 505,188 + +2.0 505,189 - +0.2 505,189 - -0.4 505,189 - +1.0 505,189 - -1.2 505,210 + -2.7 505,210 + -0.3 505,210 + -0.0 505,210 + +0.5 505,211 - -0.5 505,230 + -0.8 505,230 + -1.6 505,230 + -1.9 505,230 + +0.4 505,230 + +0.5 505,230 + -0.3 505,230 + -0.0 505,230 + -0.9 505,230 + -2.5 505,230 + -0.7 505,230 + -0.5 505,230 + -1.8 505,230 + -2.3 505,230 + -0.2 505,230 + -0.2 505,230 + -1.0 505,230 + -1.5 505,230 + -0.8 505,230 + -1.4 505,230 + +0.2 505,230 + +1.5 505,230 + -0.8 505,230 + -0.7 505,230 + -0.4 505,230 + -0.0 505,231 - -0.6 505,231 - +0.7 505,231 - -0.0 505,231 - -1.0 505,231 - -0.6 505,231 - +1.0 505,231 - -0.1 505,231 - -0.8 505,231 - +1.0 505,231 - -1.6 505,231 - +1.0 505,231 - -0.6 505,231 - -0.0 505,231 - -1.5 505,232 + -1.0 505,232 + -1.2 505,232 + -1.2 505,232 + -1.8 505,232 + -1.2 505,232 + +0.2 505,232 + -0.7 505,232 + +1.0 505,232 + -1.6 505,232 + -1.4 505,232 + +1.0 505,232 + -1.7 505,232 + -1.8 505,232 + -1.4 505,232 + -1.6 505,232 + +0.4 505,232 + -0.6 505,232 + +2.0 505,232 + -0.4 505,232 + -0.6 505,232 + -1.4 505,232 + -1.4 505,232 + -1.8 505,232 + -0.0 505,233 - +0.4 505,233 - -0.2 505,233 - -1.0 505,233 - -1.8 505,233 - -1.6 505,233 - -0.4 505,233 - -1.2 505,233 - +0.5 505,244 + -0.0 505,244 + -0.5 505,244 + +1.0 505,244 + -1.2 505,244 + -0.4 505,281 - -0.6 505,285 + -0.2 505,285 + +0.2 505,285 + -1.1 505,285 + -0.2 505,285 + +0.5 505,286 - +0.2 505,288 + -1.0 505,288 + +2.5 505,288 + -1.8 505,288 + -1.0 505,288 + +0.4 505,288 + +1.0 505,288 + -0.6 505,288 + +1.0 505,288 + +1.0 505,289 - -1.6 505,289 - -1.4 505,289 - -2.0 505,289 - +1.0 505,289 - -0.0 505,289 - +0.2 505,289 - -0.9 505,391 + -1.1 505,480 + -2.2 505,480 + -0.0 505,480 + -0.4 505,480 + -1.6 505,480 + -0.3 505,480 + -0.6 505,480 + -0.6 505,480 + -0.6 505,480 + -1.6 505,480 + -1.0 505,480 + +2.0 505,480 + -0.6 505,480 + -1.0 505,480 + -1.4 505,480 + +2.0 505,481 - -1.6 505,481 - -0.6 505,481 - -0.0 505,481 - +0.4 505,481 - -0.6 505,481 - -1.6 505,481 - -0.0 505,481 - +0.4 505,481 - +1.0 505,481 - -0.4 505,481 - -1.0 505,481 - -0.0 505,481 - -0.6 505,481 - -1.0
Or see this region's nucleotide sequence