Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS01975 and LRK53_RS01980 are separated by 56 nucleotides LRK53_RS01980 and LRK53_RS01985 are separated by 23 nucleotides
LRK53_RS01975: LRK53_RS01975 - DNA cytosine methyltransferase, at 457,241 to 458,281
_RS01975
LRK53_RS01980: LRK53_RS01980 - very short patch repair endonuclease, at 458,338 to 458,739
_RS01980
LRK53_RS01985: LRK53_RS01985 - DISARM system SNF2-like helicase DrmD, at 458,763 to 461,981
_RS01985
Position (kb) 458
459 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 457.361 kb on + strand, within LRK53_RS01975 at 457.362 kb on - strand, within LRK53_RS01975 at 457.369 kb on + strand, within LRK53_RS01975 at 457.369 kb on + strand, within LRK53_RS01975 at 457.369 kb on + strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.370 kb on - strand, within LRK53_RS01975 at 457.435 kb on - strand, within LRK53_RS01975 at 457.435 kb on - strand, within LRK53_RS01975 at 457.496 kb on + strand, within LRK53_RS01975 at 457.496 kb on + strand, within LRK53_RS01975 at 457.496 kb on + strand, within LRK53_RS01975 at 457.508 kb on + strand, within LRK53_RS01975 at 457.509 kb on - strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.547 kb on + strand, within LRK53_RS01975 at 457.548 kb on - strand, within LRK53_RS01975 at 457.614 kb on + strand, within LRK53_RS01975 at 457.648 kb on + strand, within LRK53_RS01975 at 457.648 kb on + strand, within LRK53_RS01975 at 457.668 kb on - strand, within LRK53_RS01975 at 457.703 kb on + strand, within LRK53_RS01975 at 457.703 kb on + strand, within LRK53_RS01975 at 457.703 kb on + strand, within LRK53_RS01975 at 457.704 kb on - strand, within LRK53_RS01975 at 457.760 kb on - strand, within LRK53_RS01975 at 457.822 kb on - strand, within LRK53_RS01975 at 457.822 kb on - strand, within LRK53_RS01975 at 457.822 kb on - strand, within LRK53_RS01975 at 457.822 kb on - strand, within LRK53_RS01975 at 457.991 kb on + strand, within LRK53_RS01975 at 457.991 kb on + strand, within LRK53_RS01975 at 457.992 kb on - strand, within LRK53_RS01975 at 458.003 kb on + strand, within LRK53_RS01975 at 458.003 kb on + strand, within LRK53_RS01975 at 458.004 kb on - strand, within LRK53_RS01975 at 458.054 kb on + strand, within LRK53_RS01975 at 458.054 kb on + strand, within LRK53_RS01975 at 458.054 kb on + strand, within LRK53_RS01975 at 458.054 kb on + strand, within LRK53_RS01975 at 458.054 kb on + strand, within LRK53_RS01975 at 458.054 kb on + strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.055 kb on - strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.103 kb on + strand, within LRK53_RS01975 at 458.104 kb on - strand, within LRK53_RS01975 at 458.104 kb on - strand, within LRK53_RS01975 at 458.147 kb on + strand, within LRK53_RS01975 at 458.147 kb on + strand, within LRK53_RS01975 at 458.147 kb on + strand, within LRK53_RS01975 at 458.147 kb on + strand, within LRK53_RS01975 at 458.147 kb on + strand, within LRK53_RS01975 at 458.148 kb on - strand, within LRK53_RS01975 at 458.148 kb on - strand, within LRK53_RS01975 at 458.148 kb on - strand, within LRK53_RS01975 at 458.148 kb on - strand, within LRK53_RS01975 at 458.148 kb on - strand, within LRK53_RS01975 at 458.148 kb on - strand, within LRK53_RS01975 at 458.148 kb on - strand, within LRK53_RS01975 at 458.206 kb on + strand at 458.207 kb on - strand at 458.207 kb on - strand at 458.220 kb on + strand at 458.290 kb on + strand at 458.290 kb on + strand at 458.290 kb on + strand at 458.291 kb on - strand at 458.291 kb on - strand at 458.291 kb on - strand at 458.291 kb on - strand at 458.322 kb on + strand at 458.337 kb on + strand at 458.361 kb on + strand at 458.362 kb on - strand at 458.413 kb on + strand, within LRK53_RS01980 at 458.413 kb on + strand, within LRK53_RS01980 at 458.413 kb on + strand, within LRK53_RS01980 at 458.414 kb on - strand, within LRK53_RS01980 at 458.414 kb on - strand, within LRK53_RS01980 at 458.425 kb on + strand, within LRK53_RS01980 at 458.425 kb on + strand, within LRK53_RS01980 at 458.425 kb on + strand, within LRK53_RS01980 at 458.426 kb on - strand, within LRK53_RS01980 at 458.426 kb on - strand, within LRK53_RS01980 at 458.426 kb on - strand, within LRK53_RS01980 at 458.636 kb on + strand, within LRK53_RS01980 at 458.636 kb on + strand, within LRK53_RS01980 at 458.637 kb on - strand, within LRK53_RS01980 at 458.637 kb on - strand, within LRK53_RS01980 at 458.637 kb on - strand, within LRK53_RS01980 at 458.669 kb on + strand, within LRK53_RS01980 at 458.670 kb on - strand, within LRK53_RS01980 at 458.670 kb on - strand, within LRK53_RS01980 at 458.670 kb on - strand, within LRK53_RS01980 at 458.670 kb on - strand, within LRK53_RS01980 at 458.706 kb on + strand at 458.721 kb on + strand at 458.721 kb on + strand at 458.721 kb on + strand at 458.721 kb on + strand at 458.722 kb on - strand at 458.722 kb on - strand at 458.722 kb on - strand at 458.737 kb on + strand at 458.737 kb on + strand at 458.737 kb on + strand at 458.737 kb on + strand at 458.738 kb on - strand at 458.738 kb on - strand at 458.738 kb on - strand at 458.738 kb on - strand at 458.738 kb on - strand at 458.738 kb on - strand at 458.742 kb on + strand at 458.742 kb on + strand at 458.742 kb on + strand at 458.743 kb on - strand at 458.743 kb on - strand at 458.743 kb on - strand at 458.743 kb on - strand at 458.743 kb on - strand at 458.808 kb on + strand at 458.808 kb on + strand at 458.808 kb on + strand at 458.808 kb on + strand at 458.808 kb on + strand at 458.808 kb on + strand at 458.808 kb on + strand at 458.808 kb on + strand at 458.809 kb on - strand at 458.809 kb on - strand at 458.809 kb on - strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.836 kb on + strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.837 kb on - strand at 458.839 kb on + strand at 458.839 kb on + strand at 458.839 kb on + strand at 458.839 kb on + strand at 458.845 kb on + strand at 458.845 kb on + strand at 458.845 kb on + strand at 458.845 kb on + strand at 458.846 kb on - strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.934 kb on + strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.935 kb on - strand at 458.962 kb on + strand at 458.962 kb on + strand at 458.962 kb on + strand at 458.962 kb on + strand at 458.963 kb on - strand at 458.963 kb on - strand at 458.967 kb on - strand at 458.967 kb on - strand at 458.967 kb on - strand at 459.164 kb on + strand, within LRK53_RS01985 at 459.164 kb on + strand, within LRK53_RS01985 at 459.164 kb on + strand, within LRK53_RS01985 at 459.165 kb on - strand, within LRK53_RS01985 at 459.165 kb on - strand, within LRK53_RS01985 at 459.165 kb on - strand, within LRK53_RS01985 at 459.430 kb on + strand, within LRK53_RS01985 at 459.444 kb on + strand, within LRK53_RS01985 at 459.444 kb on + strand, within LRK53_RS01985 at 459.444 kb on + strand, within LRK53_RS01985 at 459.444 kb on + strand, within LRK53_RS01985 at 459.444 kb on + strand, within LRK53_RS01985 at 459.444 kb on + strand, within LRK53_RS01985 at 459.444 kb on + strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.445 kb on - strand, within LRK53_RS01985 at 459.574 kb on + strand, within LRK53_RS01985 at 459.574 kb on + strand, within LRK53_RS01985 at 459.575 kb on - strand, within LRK53_RS01985 at 459.575 kb on - strand, within LRK53_RS01985 at 459.575 kb on - strand, within LRK53_RS01985 at 459.654 kb on + strand, within LRK53_RS01985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 457,361 + LRK53_RS01975 0.12 -0.6 457,362 - LRK53_RS01975 0.12 -1.0 457,369 + LRK53_RS01975 0.12 -2.0 457,369 + LRK53_RS01975 0.12 -0.0 457,369 + LRK53_RS01975 0.12 -1.6 457,370 - LRK53_RS01975 0.12 -0.3 457,370 - LRK53_RS01975 0.12 -1.4 457,370 - LRK53_RS01975 0.12 +0.2 457,370 - LRK53_RS01975 0.12 -0.5 457,370 - LRK53_RS01975 0.12 -0.5 457,370 - LRK53_RS01975 0.12 -1.0 457,370 - LRK53_RS01975 0.12 -1.4 457,370 - LRK53_RS01975 0.12 -0.0 457,370 - LRK53_RS01975 0.12 -1.2 457,435 - LRK53_RS01975 0.19 -1.0 457,435 - LRK53_RS01975 0.19 -0.4 457,496 + LRK53_RS01975 0.24 -0.8 457,496 + LRK53_RS01975 0.24 +1.0 457,496 + LRK53_RS01975 0.24 -0.0 457,508 + LRK53_RS01975 0.26 -2.0 457,509 - LRK53_RS01975 0.26 -0.1 457,547 + LRK53_RS01975 0.29 -1.2 457,547 + LRK53_RS01975 0.29 -0.0 457,547 + LRK53_RS01975 0.29 -1.0 457,547 + LRK53_RS01975 0.29 -0.0 457,547 + LRK53_RS01975 0.29 -0.9 457,547 + LRK53_RS01975 0.29 +0.5 457,547 + LRK53_RS01975 0.29 -1.7 457,547 + LRK53_RS01975 0.29 -2.0 457,547 + LRK53_RS01975 0.29 -1.3 457,548 - LRK53_RS01975 0.29 -0.0 457,614 + LRK53_RS01975 0.36 -1.0 457,648 + LRK53_RS01975 0.39 -1.4 457,648 + LRK53_RS01975 0.39 +2.0 457,668 - LRK53_RS01975 0.41 -0.1 457,703 + LRK53_RS01975 0.44 -0.2 457,703 + LRK53_RS01975 0.44 -1.2 457,703 + LRK53_RS01975 0.44 -0.9 457,704 - LRK53_RS01975 0.44 -0.6 457,760 - LRK53_RS01975 0.50 -1.2 457,822 - LRK53_RS01975 0.56 +1.7 457,822 - LRK53_RS01975 0.56 -2.2 457,822 - LRK53_RS01975 0.56 -0.0 457,822 - LRK53_RS01975 0.56 -0.8 457,991 + LRK53_RS01975 0.72 -1.0 457,991 + LRK53_RS01975 0.72 -1.4 457,992 - LRK53_RS01975 0.72 -1.0 458,003 + LRK53_RS01975 0.73 -0.3 458,003 + LRK53_RS01975 0.73 -1.3 458,004 - LRK53_RS01975 0.73 -0.6 458,054 + LRK53_RS01975 0.78 -1.0 458,054 + LRK53_RS01975 0.78 -1.4 458,054 + LRK53_RS01975 0.78 +1.0 458,054 + LRK53_RS01975 0.78 -0.0 458,054 + LRK53_RS01975 0.78 -2.4 458,054 + LRK53_RS01975 0.78 -2.4 458,055 - LRK53_RS01975 0.78 -1.1 458,055 - LRK53_RS01975 0.78 -0.6 458,055 - LRK53_RS01975 0.78 -0.6 458,055 - LRK53_RS01975 0.78 -0.9 458,055 - LRK53_RS01975 0.78 -1.0 458,055 - LRK53_RS01975 0.78 -0.6 458,055 - LRK53_RS01975 0.78 -0.5 458,055 - LRK53_RS01975 0.78 -1.0 458,055 - LRK53_RS01975 0.78 +0.5 458,055 - LRK53_RS01975 0.78 -0.9 458,103 + LRK53_RS01975 0.83 -1.4 458,103 + LRK53_RS01975 0.83 -0.0 458,103 + LRK53_RS01975 0.83 -1.2 458,103 + LRK53_RS01975 0.83 -1.6 458,103 + LRK53_RS01975 0.83 +0.4 458,103 + LRK53_RS01975 0.83 -1.4 458,103 + LRK53_RS01975 0.83 -0.4 458,103 + LRK53_RS01975 0.83 -1.0 458,103 + LRK53_RS01975 0.83 -0.6 458,104 - LRK53_RS01975 0.83 -0.0 458,104 - LRK53_RS01975 0.83 -1.0 458,147 + LRK53_RS01975 0.87 -0.6 458,147 + LRK53_RS01975 0.87 -0.4 458,147 + LRK53_RS01975 0.87 +1.0 458,147 + LRK53_RS01975 0.87 -0.8 458,147 + LRK53_RS01975 0.87 -1.6 458,148 - LRK53_RS01975 0.87 -1.0 458,148 - LRK53_RS01975 0.87 -0.0 458,148 - LRK53_RS01975 0.87 -0.6 458,148 - LRK53_RS01975 0.87 +0.2 458,148 - LRK53_RS01975 0.87 -0.3 458,148 - LRK53_RS01975 0.87 -0.0 458,148 - LRK53_RS01975 0.87 +0.2 458,206 + -1.4 458,207 - +0.7 458,207 - -0.4 458,220 + -0.8 458,290 + -0.8 458,290 + -1.1 458,290 + -1.7 458,291 - +0.4 458,291 - +1.0 458,291 - -1.0 458,291 - +0.4 458,322 + -3.0 458,337 + -1.5 458,361 + +0.2 458,362 - +0.2 458,413 + LRK53_RS01980 0.19 -1.4 458,413 + LRK53_RS01980 0.19 +1.0 458,413 + LRK53_RS01980 0.19 -1.6 458,414 - LRK53_RS01980 0.19 +0.7 458,414 - LRK53_RS01980 0.19 -1.4 458,425 + LRK53_RS01980 0.22 -1.3 458,425 + LRK53_RS01980 0.22 -1.0 458,425 + LRK53_RS01980 0.22 -0.6 458,426 - LRK53_RS01980 0.22 -2.6 458,426 - LRK53_RS01980 0.22 -0.6 458,426 - LRK53_RS01980 0.22 -0.5 458,636 + LRK53_RS01980 0.74 -0.0 458,636 + LRK53_RS01980 0.74 -2.4 458,637 - LRK53_RS01980 0.74 -1.2 458,637 - LRK53_RS01980 0.74 -0.2 458,637 - LRK53_RS01980 0.74 +1.0 458,669 + LRK53_RS01980 0.82 -1.0 458,670 - LRK53_RS01980 0.83 +1.0 458,670 - LRK53_RS01980 0.83 -1.6 458,670 - LRK53_RS01980 0.83 +0.4 458,670 - LRK53_RS01980 0.83 -0.6 458,706 + -1.0 458,721 + +0.4 458,721 + -0.6 458,721 + -0.9 458,721 + +1.0 458,722 - +0.6 458,722 - -0.6 458,722 - -2.2 458,737 + -1.0 458,737 + +1.0 458,737 + -0.6 458,737 + -0.0 458,738 - -1.8 458,738 - -0.5 458,738 - +0.4 458,738 - -0.8 458,738 - -1.1 458,738 - +0.4 458,742 + +1.0 458,742 + -2.1 458,742 + -0.9 458,743 - -1.0 458,743 - -0.9 458,743 - -1.1 458,743 - -0.3 458,743 - -1.7 458,808 + +0.4 458,808 + -0.4 458,808 + -1.4 458,808 + -0.4 458,808 + -0.6 458,808 + -0.8 458,808 + -1.8 458,808 + -0.6 458,809 - +1.0 458,809 - -0.3 458,809 - +1.6 458,836 + -1.5 458,836 + -2.4 458,836 + -0.0 458,836 + -0.0 458,836 + +0.4 458,836 + -0.4 458,836 + -1.0 458,836 + +3.0 458,836 + +1.0 458,837 - +3.0 458,837 - -1.2 458,837 - -0.8 458,837 - -0.0 458,837 - -1.0 458,837 - +0.4 458,837 - -1.1 458,837 - -2.0 458,837 - -2.4 458,839 + -0.4 458,839 + -2.0 458,839 + -0.0 458,839 + -0.0 458,845 + -1.3 458,845 + -0.6 458,845 + -0.0 458,845 + -1.4 458,846 - -0.4 458,934 + -0.6 458,934 + -0.9 458,934 + -0.3 458,934 + -1.8 458,934 + -0.6 458,934 + -1.0 458,934 + -0.6 458,934 + -0.0 458,934 + -0.0 458,934 + -0.0 458,934 + -1.0 458,934 + +1.4 458,934 + -0.6 458,934 + +0.2 458,934 + -1.4 458,934 + +0.7 458,934 + +1.0 458,934 + -0.0 458,935 - -0.6 458,935 - -1.4 458,935 - -1.0 458,935 - -0.0 458,935 - -0.0 458,935 - +1.0 458,935 - -1.3 458,935 - -0.6 458,935 - +1.0 458,935 - -0.4 458,962 + +1.0 458,962 + -0.7 458,962 + -0.0 458,962 + -1.0 458,963 - -1.6 458,963 - -0.8 458,967 - -0.0 458,967 - +1.0 458,967 - +0.1 459,164 + LRK53_RS01985 0.12 +0.8 459,164 + LRK53_RS01985 0.12 -1.6 459,164 + LRK53_RS01985 0.12 +0.4 459,165 - LRK53_RS01985 0.12 +1.6 459,165 - LRK53_RS01985 0.12 -1.2 459,165 - LRK53_RS01985 0.12 +1.0 459,430 + LRK53_RS01985 0.21 -0.0 459,444 + LRK53_RS01985 0.21 -0.6 459,444 + LRK53_RS01985 0.21 +0.4 459,444 + LRK53_RS01985 0.21 -0.0 459,444 + LRK53_RS01985 0.21 -1.2 459,444 + LRK53_RS01985 0.21 -1.6 459,444 + LRK53_RS01985 0.21 -0.4 459,444 + LRK53_RS01985 0.21 +0.4 459,445 - LRK53_RS01985 0.21 -0.6 459,445 - LRK53_RS01985 0.21 -1.3 459,445 - LRK53_RS01985 0.21 -1.2 459,445 - LRK53_RS01985 0.21 -1.4 459,445 - LRK53_RS01985 0.21 -0.6 459,445 - LRK53_RS01985 0.21 -0.6 459,445 - LRK53_RS01985 0.21 -0.4 459,445 - LRK53_RS01985 0.21 +0.1 459,445 - LRK53_RS01985 0.21 -1.5 459,445 - LRK53_RS01985 0.21 -1.2 459,445 - LRK53_RS01985 0.21 -0.6 459,445 - LRK53_RS01985 0.21 -0.1 459,574 + LRK53_RS01985 0.25 -0.6 459,574 + LRK53_RS01985 0.25 -0.9 459,575 - LRK53_RS01985 0.25 -0.0 459,575 - LRK53_RS01985 0.25 -1.0 459,575 - LRK53_RS01985 0.25 -0.6 459,654 + LRK53_RS01985 0.28 -1.4
Or see this region's nucleotide sequence