Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00935 and LRK53_RS00940 are separated by 151 nucleotides LRK53_RS00940 and LRK53_RS00945 are separated by 383 nucleotides LRK53_RS00945 and LRK53_RS00950 overlap by 8 nucleotides LRK53_RS00950 and LRK53_RS00955 are separated by 276 nucleotides
LRK53_RS00935: LRK53_RS00935 - monovalent cation/H(+) antiporter subunit G, at 235,859 to 236,212
_RS00935
LRK53_RS00940: LRK53_RS00940 - hypothetical protein, at 236,364 to 236,561
_RS00940
LRK53_RS00945: LRK53_RS00945 - helix-turn-helix domain-containing protein, at 236,945 to 238,063
_RS00945
LRK53_RS00950: LRK53_RS00950 - hypothetical protein, at 238,056 to 238,331
_RS00950
LRK53_RS00955: LRK53_RS00955 - IS200/IS605 family transposase, at 238,608 to 239,039
_RS00955
Position (kb) 236
237
238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 235.976 kb on + strand, within LRK53_RS00935 at 235.976 kb on + strand, within LRK53_RS00935 at 235.977 kb on - strand, within LRK53_RS00935 at 236.082 kb on - strand, within LRK53_RS00935 at 236.156 kb on + strand, within LRK53_RS00935 at 236.156 kb on + strand, within LRK53_RS00935 at 236.157 kb on - strand, within LRK53_RS00935 at 236.210 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.354 kb on - strand at 236.354 kb on - strand at 236.354 kb on - strand at 236.354 kb on - strand at 236.468 kb on - strand, within LRK53_RS00940 at 236.506 kb on + strand, within LRK53_RS00940 at 236.698 kb on - strand at 236.698 kb on - strand at 236.698 kb on - strand at 236.698 kb on - strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.899 kb on - strand at 236.899 kb on - strand at 236.899 kb on - strand at 236.916 kb on + strand at 236.916 kb on + strand at 236.916 kb on + strand at 236.916 kb on + strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.943 kb on + strand at 236.944 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 237.002 kb on + strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.112 kb on + strand, within LRK53_RS00945 at 237.113 kb on - strand, within LRK53_RS00945 at 237.126 kb on - strand, within LRK53_RS00945 at 237.126 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.309 kb on - strand, within LRK53_RS00945 at 238.067 kb on - strand at 238.067 kb on - strand at 238.067 kb on - strand at 238.067 kb on - strand at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.314 kb on + strand at 238.314 kb on + strand at 238.315 kb on - strand at 238.402 kb on + strand at 238.403 kb on - strand at 238.403 kb on - strand at 238.412 kb on + strand at 238.423 kb on + strand at 238.487 kb on - strand at 238.487 kb on - strand at 238.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 235,976 + LRK53_RS00935 0.33 +0.4 235,976 + LRK53_RS00935 0.33 -0.7 235,977 - LRK53_RS00935 0.33 -0.9 236,082 - LRK53_RS00935 0.63 -0.6 236,156 + LRK53_RS00935 0.84 -1.2 236,156 + LRK53_RS00935 0.84 +0.7 236,157 - LRK53_RS00935 0.84 -0.6 236,210 + -2.2 236,280 + -0.6 236,280 + -0.7 236,280 + -1.0 236,280 + -0.6 236,280 + +0.0 236,281 - +1.0 236,281 - +1.0 236,281 - -0.5 236,281 - -0.8 236,281 - -0.8 236,281 - +1.0 236,353 + -1.3 236,353 + +1.0 236,353 + -1.5 236,353 + -0.2 236,353 + +2.0 236,354 - +0.0 236,354 - -1.8 236,354 - +1.0 236,354 - +0.0 236,468 - LRK53_RS00940 0.53 -0.4 236,506 + LRK53_RS00940 0.72 -1.6 236,698 - -1.7 236,698 - +0.0 236,698 - +1.0 236,698 - -1.0 236,750 + -0.6 236,750 + +0.4 236,750 + +0.0 236,750 + +0.6 236,750 + -1.6 236,750 + -1.2 236,750 + +0.1 236,750 + +1.0 236,751 - +0.3 236,751 - -1.6 236,751 - -1.6 236,751 - +0.1 236,751 - -0.6 236,751 - +0.4 236,751 - -1.0 236,751 - +0.0 236,751 - -0.6 236,751 - +1.4 236,751 - -1.3 236,751 - +0.4 236,751 - -1.2 236,751 - +1.6 236,899 - -1.0 236,899 - -0.6 236,899 - +0.4 236,916 + -2.2 236,916 + -0.3 236,916 + -0.3 236,916 + -0.6 236,917 - -0.6 236,917 - -0.6 236,917 - -1.1 236,917 - +0.4 236,917 - -0.6 236,943 + +0.5 236,944 - +0.6 236,979 - -1.0 236,979 - +0.0 236,979 - -1.6 236,979 - -0.6 236,979 - +1.0 236,979 - -0.7 236,979 - -0.3 236,979 - -1.8 237,002 + -0.9 237,003 - -0.1 237,003 - +0.0 237,003 - -1.3 237,003 - -0.8 237,003 - -0.8 237,003 - +0.1 237,003 - -0.3 237,003 - -0.3 237,003 - +1.0 237,054 - -1.7 237,054 - -0.3 237,054 - +0.6 237,054 - +2.0 237,054 - +0.4 237,054 - -2.2 237,054 - -2.6 237,054 - -1.6 237,054 - -0.4 237,054 - -2.8 237,054 - +0.3 237,054 - -0.3 237,054 - -0.4 237,054 - -0.8 237,054 - +0.5 237,054 - -2.3 237,112 + LRK53_RS00945 0.15 -1.0 237,113 - LRK53_RS00945 0.15 -1.0 237,126 - LRK53_RS00945 0.16 -1.6 237,126 - LRK53_RS00945 0.16 +0.4 237,207 - LRK53_RS00945 0.23 -0.3 237,207 - LRK53_RS00945 0.23 -1.0 237,207 - LRK53_RS00945 0.23 -0.6 237,207 - LRK53_RS00945 0.23 -1.0 237,207 - LRK53_RS00945 0.23 +0.4 237,309 - LRK53_RS00945 0.33 +0.4 238,067 - -1.8 238,067 - +1.3 238,067 - -1.0 238,067 - +1.0 238,288 + LRK53_RS00950 0.84 +0.2 238,288 + LRK53_RS00950 0.84 -0.6 238,288 + LRK53_RS00950 0.84 -1.7 238,288 + LRK53_RS00950 0.84 -1.3 238,288 + LRK53_RS00950 0.84 -0.5 238,288 + LRK53_RS00950 0.84 +0.1 238,288 + LRK53_RS00950 0.84 +0.4 238,288 + LRK53_RS00950 0.84 -0.4 238,288 + LRK53_RS00950 0.84 -0.6 238,288 + LRK53_RS00950 0.84 +1.0 238,288 + LRK53_RS00950 0.84 -1.7 238,288 + LRK53_RS00950 0.84 +0.8 238,288 + LRK53_RS00950 0.84 -1.0 238,289 - LRK53_RS00950 0.84 -0.3 238,289 - LRK53_RS00950 0.84 -1.0 238,289 - LRK53_RS00950 0.84 -0.6 238,289 - LRK53_RS00950 0.84 +0.4 238,289 - LRK53_RS00950 0.84 -1.2 238,289 - LRK53_RS00950 0.84 -2.8 238,289 - LRK53_RS00950 0.84 -0.9 238,289 - LRK53_RS00950 0.84 -0.2 238,314 + -0.6 238,314 + +0.0 238,315 - +1.6 238,402 + +0.0 238,403 - -0.6 238,403 - -0.6 238,412 + +1.0 238,423 + +1.0 238,487 - +1.0 238,487 - -0.3 238,487 - -1.3
Or see this region's nucleotide sequence