Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00735 and LRK53_RS00740 are separated by 22 nucleotides LRK53_RS00740 and LRK53_RS00745 are separated by 52 nucleotides LRK53_RS00745 and LRK53_RS00750 are separated by 163 nucleotides
LRK53_RS00735: LRK53_RS00735 - efflux transporter outer membrane subunit, at 173,154 to 174,605
_RS00735
LRK53_RS00740: LRK53_RS00740 - MarR family transcriptional regulator, at 174,628 to 175,086
_RS00740
LRK53_RS00745: LRK53_RS00745 - hypothetical protein, at 175,139 to 175,309
_RS00745
LRK53_RS00750: LRK53_RS00750 - efflux RND transporter permease subunit, at 175,473 to 178,715
_RS00750
Position (kb) 174
175
176 Strain fitness (log2 ratio) -2
-1
0
1
2 at 174.055 kb on + strand, within LRK53_RS00735 at 174.055 kb on + strand, within LRK53_RS00735 at 174.055 kb on + strand, within LRK53_RS00735 at 174.056 kb on - strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.070 kb on + strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.071 kb on - strand, within LRK53_RS00735 at 174.208 kb on + strand, within LRK53_RS00735 at 174.208 kb on + strand, within LRK53_RS00735 at 174.208 kb on + strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.209 kb on - strand, within LRK53_RS00735 at 174.214 kb on + strand, within LRK53_RS00735 at 174.214 kb on + strand, within LRK53_RS00735 at 174.215 kb on - strand, within LRK53_RS00735 at 174.215 kb on - strand, within LRK53_RS00735 at 174.215 kb on - strand, within LRK53_RS00735 at 174.215 kb on - strand, within LRK53_RS00735 at 174.215 kb on - strand, within LRK53_RS00735 at 174.215 kb on - strand, within LRK53_RS00735 at 174.668 kb on + strand at 174.668 kb on + strand at 174.668 kb on + strand at 174.668 kb on + strand at 174.668 kb on + strand at 174.668 kb on + strand at 174.668 kb on + strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.669 kb on - strand at 174.830 kb on + strand, within LRK53_RS00740 at 174.830 kb on + strand, within LRK53_RS00740 at 174.831 kb on - strand, within LRK53_RS00740 at 174.831 kb on - strand, within LRK53_RS00740 at 174.831 kb on - strand, within LRK53_RS00740 at 174.831 kb on - strand, within LRK53_RS00740 at 174.831 kb on - strand, within LRK53_RS00740 at 175.081 kb on - strand at 175.081 kb on - strand at 175.099 kb on + strand at 175.099 kb on + strand at 175.099 kb on + strand at 175.100 kb on - strand at 175.100 kb on - strand at 175.344 kb on + strand at 175.344 kb on + strand at 175.376 kb on + strand at 175.376 kb on + strand at 175.376 kb on + strand at 175.377 kb on - strand at 175.377 kb on - strand at 175.377 kb on - strand at 175.377 kb on - strand at 175.377 kb on - strand at 175.377 kb on - strand at 175.377 kb on - strand at 175.403 kb on + strand at 175.403 kb on + strand at 175.404 kb on - strand at 175.404 kb on - strand at 175.404 kb on - strand at 175.528 kb on + strand at 175.529 kb on - strand at 175.531 kb on + strand at 175.531 kb on + strand at 175.531 kb on + strand at 175.531 kb on + strand at 175.531 kb on + strand at 175.531 kb on + strand at 175.531 kb on + strand at 175.531 kb on + strand at 175.532 kb on - strand at 175.532 kb on - strand at 175.532 kb on - strand at 175.532 kb on - strand at 175.532 kb on - strand at 175.532 kb on - strand at 175.532 kb on - strand at 175.532 kb on - strand at 175.584 kb on + strand at 175.584 kb on + strand at 175.584 kb on + strand at 175.584 kb on + strand at 175.585 kb on - strand at 175.585 kb on - strand at 175.585 kb on - strand at 175.906 kb on + strand, within LRK53_RS00750 at 175.906 kb on + strand, within LRK53_RS00750 at 175.906 kb on + strand, within LRK53_RS00750 at 175.906 kb on + strand, within LRK53_RS00750 at 175.906 kb on + strand, within LRK53_RS00750 at 175.906 kb on + strand, within LRK53_RS00750 at 175.907 kb on - strand, within LRK53_RS00750 at 175.907 kb on - strand, within LRK53_RS00750 at 175.907 kb on - strand, within LRK53_RS00750 at 175.936 kb on + strand, within LRK53_RS00750 at 175.936 kb on + strand, within LRK53_RS00750 at 175.936 kb on + strand, within LRK53_RS00750 at 175.936 kb on + strand, within LRK53_RS00750 at 175.936 kb on + strand, within LRK53_RS00750 at 175.936 kb on + strand, within LRK53_RS00750 at 175.936 kb on + strand, within LRK53_RS00750 at 175.937 kb on - strand, within LRK53_RS00750 at 175.937 kb on - strand, within LRK53_RS00750 at 175.937 kb on - strand, within LRK53_RS00750 at 175.937 kb on - strand, within LRK53_RS00750 at 175.961 kb on - strand, within LRK53_RS00750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 174,055 + LRK53_RS00735 0.62 -2.3 174,055 + LRK53_RS00735 0.62 -0.7 174,055 + LRK53_RS00735 0.62 -1.0 174,056 - LRK53_RS00735 0.62 -2.0 174,070 + LRK53_RS00735 0.63 -0.6 174,070 + LRK53_RS00735 0.63 -1.0 174,070 + LRK53_RS00735 0.63 -0.3 174,070 + LRK53_RS00735 0.63 -0.5 174,070 + LRK53_RS00735 0.63 +0.6 174,070 + LRK53_RS00735 0.63 -0.3 174,070 + LRK53_RS00735 0.63 -1.2 174,070 + LRK53_RS00735 0.63 +0.0 174,070 + LRK53_RS00735 0.63 -1.3 174,070 + LRK53_RS00735 0.63 -1.7 174,070 + LRK53_RS00735 0.63 +0.3 174,070 + LRK53_RS00735 0.63 -0.7 174,070 + LRK53_RS00735 0.63 -1.3 174,070 + LRK53_RS00735 0.63 -0.9 174,070 + LRK53_RS00735 0.63 +0.8 174,070 + LRK53_RS00735 0.63 -2.0 174,070 + LRK53_RS00735 0.63 +0.6 174,070 + LRK53_RS00735 0.63 -2.0 174,071 - LRK53_RS00735 0.63 -1.3 174,071 - LRK53_RS00735 0.63 +0.0 174,071 - LRK53_RS00735 0.63 -1.6 174,071 - LRK53_RS00735 0.63 -1.0 174,071 - LRK53_RS00735 0.63 -0.6 174,071 - LRK53_RS00735 0.63 -0.3 174,071 - LRK53_RS00735 0.63 -0.6 174,071 - LRK53_RS00735 0.63 -0.6 174,071 - LRK53_RS00735 0.63 +0.0 174,071 - LRK53_RS00735 0.63 +0.0 174,071 - LRK53_RS00735 0.63 +0.0 174,071 - LRK53_RS00735 0.63 +0.7 174,071 - LRK53_RS00735 0.63 -2.3 174,071 - LRK53_RS00735 0.63 -0.5 174,071 - LRK53_RS00735 0.63 -0.9 174,071 - LRK53_RS00735 0.63 -0.9 174,071 - LRK53_RS00735 0.63 +0.0 174,071 - LRK53_RS00735 0.63 -1.7 174,071 - LRK53_RS00735 0.63 -1.0 174,071 - LRK53_RS00735 0.63 +1.0 174,071 - LRK53_RS00735 0.63 -2.3 174,071 - LRK53_RS00735 0.63 +0.4 174,071 - LRK53_RS00735 0.63 -2.0 174,071 - LRK53_RS00735 0.63 -0.2 174,071 - LRK53_RS00735 0.63 -1.2 174,208 + LRK53_RS00735 0.73 -0.9 174,208 + LRK53_RS00735 0.73 +1.0 174,208 + LRK53_RS00735 0.73 -0.6 174,209 - LRK53_RS00735 0.73 -0.6 174,209 - LRK53_RS00735 0.73 -0.2 174,209 - LRK53_RS00735 0.73 +1.0 174,209 - LRK53_RS00735 0.73 -1.4 174,209 - LRK53_RS00735 0.73 -0.2 174,209 - LRK53_RS00735 0.73 -0.9 174,209 - LRK53_RS00735 0.73 -0.2 174,209 - LRK53_RS00735 0.73 -1.1 174,214 + LRK53_RS00735 0.73 +0.5 174,214 + LRK53_RS00735 0.73 +1.4 174,215 - LRK53_RS00735 0.73 -1.2 174,215 - LRK53_RS00735 0.73 -0.3 174,215 - LRK53_RS00735 0.73 -0.6 174,215 - LRK53_RS00735 0.73 -1.0 174,215 - LRK53_RS00735 0.73 -0.5 174,215 - LRK53_RS00735 0.73 -2.2 174,668 + +2.0 174,668 + -1.2 174,668 + -1.2 174,668 + -1.2 174,668 + -1.3 174,668 + +0.2 174,668 + -1.3 174,669 - -0.8 174,669 - -0.6 174,669 - -1.0 174,669 - -1.3 174,669 - -0.6 174,669 - -1.3 174,669 - -2.6 174,669 - -0.8 174,669 - -2.2 174,669 - -0.9 174,669 - +1.0 174,669 - +2.0 174,669 - -1.0 174,669 - -0.6 174,830 + LRK53_RS00740 0.44 -0.2 174,830 + LRK53_RS00740 0.44 -1.6 174,831 - LRK53_RS00740 0.44 -2.7 174,831 - LRK53_RS00740 0.44 -1.2 174,831 - LRK53_RS00740 0.44 -0.8 174,831 - LRK53_RS00740 0.44 -0.9 174,831 - LRK53_RS00740 0.44 -0.6 175,081 - -1.2 175,081 - -1.0 175,099 + -0.6 175,099 + -0.1 175,099 + +2.0 175,100 - -0.8 175,100 - +2.6 175,344 + -2.0 175,344 + -1.6 175,376 + +1.0 175,376 + -0.6 175,376 + -0.6 175,377 - -0.7 175,377 - -1.0 175,377 - -1.2 175,377 - +0.4 175,377 - -1.1 175,377 - -1.2 175,377 - +1.0 175,403 + -0.7 175,403 + -0.6 175,404 - +0.0 175,404 - -1.1 175,404 - -1.3 175,528 + -1.0 175,529 - -0.6 175,531 + -0.8 175,531 + -1.0 175,531 + -0.9 175,531 + -1.4 175,531 + -0.3 175,531 + +0.0 175,531 + +1.0 175,531 + +1.0 175,532 - -1.0 175,532 - -0.6 175,532 - +1.0 175,532 - -1.3 175,532 - +1.0 175,532 - -1.8 175,532 - -0.3 175,532 - +1.0 175,584 + -2.3 175,584 + -0.6 175,584 + +0.1 175,584 + +1.0 175,585 - -0.3 175,585 - +0.0 175,585 - -0.4 175,906 + LRK53_RS00750 0.13 +1.7 175,906 + LRK53_RS00750 0.13 +1.0 175,906 + LRK53_RS00750 0.13 +0.2 175,906 + LRK53_RS00750 0.13 +0.0 175,906 + LRK53_RS00750 0.13 -0.7 175,906 + LRK53_RS00750 0.13 -2.4 175,907 - LRK53_RS00750 0.13 +1.4 175,907 - LRK53_RS00750 0.13 -1.8 175,907 - LRK53_RS00750 0.13 +1.0 175,936 + LRK53_RS00750 0.14 -1.0 175,936 + LRK53_RS00750 0.14 +0.4 175,936 + LRK53_RS00750 0.14 -1.2 175,936 + LRK53_RS00750 0.14 -1.8 175,936 + LRK53_RS00750 0.14 +0.0 175,936 + LRK53_RS00750 0.14 +1.0 175,936 + LRK53_RS00750 0.14 +0.0 175,937 - LRK53_RS00750 0.14 -0.3 175,937 - LRK53_RS00750 0.14 -1.3 175,937 - LRK53_RS00750 0.14 -2.3 175,937 - LRK53_RS00750 0.14 -1.0 175,961 - LRK53_RS00750 0.15 -1.1
Or see this region's nucleotide sequence