Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00390 and LRK53_RS00395 are separated by 217 nucleotides LRK53_RS00395 and LRK53_RS00400 are separated by 287 nucleotides
LRK53_RS00390: LRK53_RS00390 - 4,5-DOPA dioxygenase extradiol, at 88,534 to 89,331
_RS00390
LRK53_RS00395: LRK53_RS00395 - SDR family oxidoreductase, at 89,549 to 90,313
_RS00395
LRK53_RS00400: LRK53_RS00400 - amidohydrolase family protein, at 90,601 to 91,839
_RS00400
Position (kb) 89
90
91 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 88.549 kb on - strand at 88.696 kb on + strand, within LRK53_RS00390 at 88.696 kb on + strand, within LRK53_RS00390 at 88.697 kb on - strand, within LRK53_RS00390 at 88.697 kb on - strand, within LRK53_RS00390 at 88.697 kb on - strand, within LRK53_RS00390 at 88.697 kb on - strand, within LRK53_RS00390 at 88.697 kb on - strand, within LRK53_RS00390 at 88.786 kb on + strand, within LRK53_RS00390 at 88.787 kb on - strand, within LRK53_RS00390 at 88.787 kb on - strand, within LRK53_RS00390 at 88.909 kb on + strand, within LRK53_RS00390 at 88.910 kb on - strand, within LRK53_RS00390 at 88.910 kb on - strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.062 kb on + strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.063 kb on - strand, within LRK53_RS00390 at 89.167 kb on + strand, within LRK53_RS00390 at 89.167 kb on + strand, within LRK53_RS00390 at 89.167 kb on + strand, within LRK53_RS00390 at 89.167 kb on + strand, within LRK53_RS00390 at 89.167 kb on + strand, within LRK53_RS00390 at 89.167 kb on + strand, within LRK53_RS00390 at 89.167 kb on + strand, within LRK53_RS00390 at 89.168 kb on - strand, within LRK53_RS00390 at 89.168 kb on - strand, within LRK53_RS00390 at 89.168 kb on - strand, within LRK53_RS00390 at 89.218 kb on + strand, within LRK53_RS00390 at 89.218 kb on + strand, within LRK53_RS00390 at 89.219 kb on - strand, within LRK53_RS00390 at 89.219 kb on - strand, within LRK53_RS00390 at 89.219 kb on - strand, within LRK53_RS00390 at 89.219 kb on - strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.233 kb on + strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.234 kb on - strand, within LRK53_RS00390 at 89.287 kb on - strand at 89.343 kb on + strand at 89.343 kb on + strand at 89.344 kb on - strand at 89.344 kb on - strand at 89.484 kb on + strand at 89.484 kb on + strand at 89.485 kb on - strand at 89.485 kb on - strand at 89.485 kb on - strand at 89.485 kb on - strand at 89.748 kb on - strand, within LRK53_RS00395 at 89.748 kb on - strand, within LRK53_RS00395 at 89.872 kb on - strand, within LRK53_RS00395 at 89.872 kb on - strand, within LRK53_RS00395 at 89.872 kb on - strand, within LRK53_RS00395 at 89.952 kb on + strand, within LRK53_RS00395 at 89.953 kb on - strand, within LRK53_RS00395 at 89.953 kb on - strand, within LRK53_RS00395 at 90.077 kb on + strand, within LRK53_RS00395 at 90.078 kb on - strand, within LRK53_RS00395 at 90.078 kb on - strand, within LRK53_RS00395 at 90.385 kb on + strand at 90.385 kb on + strand at 90.385 kb on + strand at 90.385 kb on + strand at 90.385 kb on + strand at 90.385 kb on + strand at 90.385 kb on + strand at 90.386 kb on - strand at 90.386 kb on - strand at 90.386 kb on - strand at 90.386 kb on - strand at 90.386 kb on - strand at 90.386 kb on - strand at 90.641 kb on + strand at 90.641 kb on + strand at 90.642 kb on - strand at 90.792 kb on + strand, within LRK53_RS00400 at 90.893 kb on + strand, within LRK53_RS00400 at 91.094 kb on + strand, within LRK53_RS00400 at 91.094 kb on + strand, within LRK53_RS00400 at 91.094 kb on + strand, within LRK53_RS00400 at 91.094 kb on + strand, within LRK53_RS00400 at 91.094 kb on + strand, within LRK53_RS00400 at 91.094 kb on + strand, within LRK53_RS00400 at 91.095 kb on - strand, within LRK53_RS00400 at 91.095 kb on - strand, within LRK53_RS00400 at 91.095 kb on - strand, within LRK53_RS00400 at 91.262 kb on + strand, within LRK53_RS00400 at 91.263 kb on - strand, within LRK53_RS00400 at 91.263 kb on - strand, within LRK53_RS00400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 88,549 - -1.0 88,696 + LRK53_RS00390 0.20 +0.6 88,696 + LRK53_RS00390 0.20 -0.8 88,697 - LRK53_RS00390 0.20 +0.6 88,697 - LRK53_RS00390 0.20 -1.0 88,697 - LRK53_RS00390 0.20 +0.2 88,697 - LRK53_RS00390 0.20 +1.0 88,697 - LRK53_RS00390 0.20 -0.3 88,786 + LRK53_RS00390 0.32 +3.0 88,787 - LRK53_RS00390 0.32 +0.0 88,787 - LRK53_RS00390 0.32 -1.8 88,909 + LRK53_RS00390 0.47 -1.0 88,910 - LRK53_RS00390 0.47 -1.8 88,910 - LRK53_RS00390 0.47 +1.0 89,062 + LRK53_RS00390 0.66 -2.0 89,062 + LRK53_RS00390 0.66 -1.8 89,062 + LRK53_RS00390 0.66 +1.0 89,062 + LRK53_RS00390 0.66 +0.0 89,062 + LRK53_RS00390 0.66 +0.0 89,062 + LRK53_RS00390 0.66 -0.2 89,062 + LRK53_RS00390 0.66 -0.7 89,062 + LRK53_RS00390 0.66 -0.6 89,062 + LRK53_RS00390 0.66 +0.4 89,062 + LRK53_RS00390 0.66 -1.6 89,062 + LRK53_RS00390 0.66 -0.6 89,062 + LRK53_RS00390 0.66 -0.6 89,062 + LRK53_RS00390 0.66 -0.8 89,062 + LRK53_RS00390 0.66 +1.0 89,062 + LRK53_RS00390 0.66 -1.0 89,062 + LRK53_RS00390 0.66 -1.0 89,062 + LRK53_RS00390 0.66 -0.6 89,062 + LRK53_RS00390 0.66 -0.8 89,062 + LRK53_RS00390 0.66 +0.3 89,062 + LRK53_RS00390 0.66 -1.0 89,062 + LRK53_RS00390 0.66 +1.0 89,062 + LRK53_RS00390 0.66 +2.0 89,062 + LRK53_RS00390 0.66 -1.3 89,062 + LRK53_RS00390 0.66 -2.3 89,062 + LRK53_RS00390 0.66 -1.3 89,062 + LRK53_RS00390 0.66 +0.4 89,062 + LRK53_RS00390 0.66 -1.4 89,062 + LRK53_RS00390 0.66 +1.6 89,062 + LRK53_RS00390 0.66 +0.8 89,062 + LRK53_RS00390 0.66 -2.0 89,062 + LRK53_RS00390 0.66 +2.0 89,062 + LRK53_RS00390 0.66 +0.4 89,062 + LRK53_RS00390 0.66 +0.0 89,062 + LRK53_RS00390 0.66 -0.2 89,063 - LRK53_RS00390 0.66 -0.4 89,063 - LRK53_RS00390 0.66 +0.0 89,063 - LRK53_RS00390 0.66 -0.7 89,063 - LRK53_RS00390 0.66 -0.6 89,063 - LRK53_RS00390 0.66 -0.6 89,063 - LRK53_RS00390 0.66 -2.4 89,063 - LRK53_RS00390 0.66 -0.8 89,063 - LRK53_RS00390 0.66 -0.6 89,063 - LRK53_RS00390 0.66 -0.6 89,063 - LRK53_RS00390 0.66 +0.7 89,063 - LRK53_RS00390 0.66 -1.3 89,063 - LRK53_RS00390 0.66 +1.6 89,063 - LRK53_RS00390 0.66 -1.0 89,063 - LRK53_RS00390 0.66 +0.3 89,063 - LRK53_RS00390 0.66 -0.6 89,063 - LRK53_RS00390 0.66 -1.1 89,063 - LRK53_RS00390 0.66 -1.3 89,063 - LRK53_RS00390 0.66 -0.6 89,063 - LRK53_RS00390 0.66 -0.2 89,063 - LRK53_RS00390 0.66 +1.0 89,167 + LRK53_RS00390 0.79 -0.8 89,167 + LRK53_RS00390 0.79 -1.8 89,167 + LRK53_RS00390 0.79 -0.2 89,167 + LRK53_RS00390 0.79 +1.0 89,167 + LRK53_RS00390 0.79 -0.5 89,167 + LRK53_RS00390 0.79 -0.6 89,167 + LRK53_RS00390 0.79 -1.6 89,168 - LRK53_RS00390 0.79 -1.3 89,168 - LRK53_RS00390 0.79 +0.8 89,168 - LRK53_RS00390 0.79 -1.6 89,218 + LRK53_RS00390 0.86 -0.2 89,218 + LRK53_RS00390 0.86 -0.6 89,219 - LRK53_RS00390 0.86 -0.7 89,219 - LRK53_RS00390 0.86 +0.0 89,219 - LRK53_RS00390 0.86 -0.3 89,219 - LRK53_RS00390 0.86 -0.8 89,233 + LRK53_RS00390 0.88 +1.0 89,233 + LRK53_RS00390 0.88 -1.3 89,233 + LRK53_RS00390 0.88 -0.8 89,233 + LRK53_RS00390 0.88 -0.8 89,233 + LRK53_RS00390 0.88 -1.0 89,233 + LRK53_RS00390 0.88 -1.0 89,233 + LRK53_RS00390 0.88 -1.4 89,233 + LRK53_RS00390 0.88 -0.3 89,234 - LRK53_RS00390 0.88 +0.2 89,234 - LRK53_RS00390 0.88 -1.0 89,234 - LRK53_RS00390 0.88 +1.0 89,234 - LRK53_RS00390 0.88 +0.4 89,234 - LRK53_RS00390 0.88 -1.0 89,234 - LRK53_RS00390 0.88 +3.0 89,234 - LRK53_RS00390 0.88 -0.6 89,234 - LRK53_RS00390 0.88 +1.0 89,234 - LRK53_RS00390 0.88 +1.0 89,234 - LRK53_RS00390 0.88 -0.7 89,234 - LRK53_RS00390 0.88 +1.0 89,234 - LRK53_RS00390 0.88 +0.0 89,234 - LRK53_RS00390 0.88 -0.5 89,287 - +0.3 89,343 + +0.0 89,343 + +0.0 89,344 - +1.6 89,344 - +0.0 89,484 + -1.5 89,484 + -0.6 89,485 - -0.3 89,485 - +1.0 89,485 - -0.6 89,485 - +1.0 89,748 - LRK53_RS00395 0.26 -2.3 89,748 - LRK53_RS00395 0.26 -1.3 89,872 - LRK53_RS00395 0.42 -0.6 89,872 - LRK53_RS00395 0.42 -1.8 89,872 - LRK53_RS00395 0.42 +0.0 89,952 + LRK53_RS00395 0.53 -0.3 89,953 - LRK53_RS00395 0.53 -1.8 89,953 - LRK53_RS00395 0.53 -1.0 90,077 + LRK53_RS00395 0.69 -1.2 90,078 - LRK53_RS00395 0.69 -0.8 90,078 - LRK53_RS00395 0.69 +0.5 90,385 + -1.8 90,385 + +2.0 90,385 + -0.8 90,385 + +0.0 90,385 + +0.0 90,385 + -0.6 90,385 + -0.9 90,386 - -1.0 90,386 - -0.9 90,386 - -0.6 90,386 - +0.6 90,386 - -1.0 90,386 - -0.8 90,641 + +1.0 90,641 + +1.0 90,642 - -0.6 90,792 + LRK53_RS00400 0.15 -0.6 90,893 + LRK53_RS00400 0.24 -1.0 91,094 + LRK53_RS00400 0.40 -1.0 91,094 + LRK53_RS00400 0.40 -1.0 91,094 + LRK53_RS00400 0.40 -0.3 91,094 + LRK53_RS00400 0.40 +0.6 91,094 + LRK53_RS00400 0.40 -0.3 91,094 + LRK53_RS00400 0.40 -0.9 91,095 - LRK53_RS00400 0.40 -0.3 91,095 - LRK53_RS00400 0.40 -1.0 91,095 - LRK53_RS00400 0.40 -0.4 91,262 + LRK53_RS00400 0.53 -0.7 91,263 - LRK53_RS00400 0.53 -2.2 91,263 - LRK53_RS00400 0.53 +0.0
Or see this region's nucleotide sequence