Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16350 and LRK53_RS16355 are separated by 506 nucleotides LRK53_RS16355 and LRK53_RS16360 are separated by 216 nucleotides LRK53_RS16360 and LRK53_RS16365 are separated by 6 nucleotides
LRK53_RS16350: LRK53_RS16350 - group II intron reverse transcriptase/maturase, at 3,598,708 to 3,600,036
_RS16350
LRK53_RS16355: LRK53_RS16355 - transposase domain-containing protein, at 3,600,543 to 3,600,764
_RS16355
LRK53_RS16360: LRK53_RS16360 - VOC family protein, at 3,600,981 to 3,601,421
_RS16360
LRK53_RS16365: LRK53_RS16365 - sigma 54-interacting transcriptional regulator, at 3,601,428 to 3,603,281
_RS16365
Position (kb)
3600
3601
3602 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3600.028 kb on + strand at 3600.028 kb on + strand at 3600.028 kb on + strand at 3600.029 kb on - strand at 3600.029 kb on - strand at 3600.029 kb on - strand at 3600.029 kb on - strand at 3600.124 kb on - strand at 3600.124 kb on - strand at 3600.126 kb on + strand at 3600.126 kb on + strand at 3600.126 kb on + strand at 3600.127 kb on - strand at 3600.127 kb on - strand at 3600.127 kb on - strand at 3600.127 kb on - strand at 3600.240 kb on + strand at 3600.241 kb on - strand at 3600.241 kb on - strand at 3600.241 kb on - strand at 3600.241 kb on - strand at 3600.393 kb on + strand at 3600.529 kb on + strand at 3600.529 kb on + strand at 3600.715 kb on + strand, within LRK53_RS16355 at 3600.749 kb on - strand at 3600.749 kb on - strand at 3600.762 kb on + strand at 3600.763 kb on - strand at 3600.763 kb on - strand at 3600.802 kb on + strand at 3600.850 kb on + strand at 3600.850 kb on + strand at 3600.851 kb on - strand at 3600.851 kb on - strand at 3601.042 kb on + strand, within LRK53_RS16360 at 3601.042 kb on + strand, within LRK53_RS16360 at 3601.042 kb on + strand, within LRK53_RS16360 at 3601.043 kb on - strand, within LRK53_RS16360 at 3601.043 kb on - strand, within LRK53_RS16360 at 3601.043 kb on - strand, within LRK53_RS16360 at 3601.043 kb on - strand, within LRK53_RS16360 at 3601.043 kb on - strand, within LRK53_RS16360 at 3601.063 kb on + strand, within LRK53_RS16360 at 3601.063 kb on + strand, within LRK53_RS16360 at 3601.063 kb on + strand, within LRK53_RS16360 at 3601.064 kb on - strand, within LRK53_RS16360 at 3601.064 kb on - strand, within LRK53_RS16360 at 3601.064 kb on - strand, within LRK53_RS16360 at 3601.064 kb on - strand, within LRK53_RS16360 at 3601.064 kb on - strand, within LRK53_RS16360 at 3601.104 kb on + strand, within LRK53_RS16360 at 3601.104 kb on + strand, within LRK53_RS16360 at 3601.105 kb on - strand, within LRK53_RS16360 at 3601.117 kb on + strand, within LRK53_RS16360 at 3601.117 kb on + strand, within LRK53_RS16360 at 3601.118 kb on - strand, within LRK53_RS16360 at 3601.118 kb on - strand, within LRK53_RS16360 at 3601.118 kb on - strand, within LRK53_RS16360 at 3601.121 kb on + strand, within LRK53_RS16360 at 3601.121 kb on + strand, within LRK53_RS16360 at 3601.121 kb on + strand, within LRK53_RS16360 at 3601.121 kb on + strand, within LRK53_RS16360 at 3601.121 kb on + strand, within LRK53_RS16360 at 3601.122 kb on - strand, within LRK53_RS16360 at 3601.122 kb on - strand, within LRK53_RS16360 at 3601.122 kb on - strand, within LRK53_RS16360 at 3601.122 kb on - strand, within LRK53_RS16360 at 3601.122 kb on - strand, within LRK53_RS16360 at 3601.122 kb on - strand, within LRK53_RS16360 at 3601.156 kb on + strand, within LRK53_RS16360 at 3601.156 kb on + strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.157 kb on - strand, within LRK53_RS16360 at 3601.218 kb on + strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.219 kb on - strand, within LRK53_RS16360 at 3601.257 kb on + strand, within LRK53_RS16360 at 3601.257 kb on + strand, within LRK53_RS16360 at 3601.257 kb on + strand, within LRK53_RS16360 at 3601.257 kb on + strand, within LRK53_RS16360 at 3601.258 kb on - strand, within LRK53_RS16360 at 3601.258 kb on - strand, within LRK53_RS16360 at 3601.461 kb on + strand at 3601.478 kb on + strand at 3601.478 kb on + strand at 3601.478 kb on + strand at 3601.479 kb on - strand at 3601.479 kb on - strand at 3601.486 kb on + strand at 3601.487 kb on - strand at 3601.600 kb on + strand at 3601.600 kb on + strand at 3601.600 kb on + strand at 3601.600 kb on + strand at 3601.601 kb on - strand at 3601.601 kb on - strand at 3601.601 kb on - strand at 3601.627 kb on + strand, within LRK53_RS16365 at 3601.628 kb on - strand, within LRK53_RS16365 at 3601.628 kb on - strand, within LRK53_RS16365 at 3601.783 kb on + strand, within LRK53_RS16365 at 3601.783 kb on + strand, within LRK53_RS16365 at 3601.783 kb on + strand, within LRK53_RS16365 at 3601.783 kb on + strand, within LRK53_RS16365 at 3601.811 kb on - strand, within LRK53_RS16365 at 3601.811 kb on - strand, within LRK53_RS16365 at 3601.874 kb on + strand, within LRK53_RS16365 at 3601.875 kb on - strand, within LRK53_RS16365 at 3601.875 kb on - strand, within LRK53_RS16365 at 3601.875 kb on - strand, within LRK53_RS16365 at 3601.888 kb on + strand, within LRK53_RS16365 at 3601.888 kb on + strand, within LRK53_RS16365 at 3601.888 kb on + strand, within LRK53_RS16365 at 3601.889 kb on - strand, within LRK53_RS16365 at 3601.889 kb on - strand, within LRK53_RS16365 at 3602.150 kb on - strand, within LRK53_RS16365 at 3602.314 kb on + strand, within LRK53_RS16365 at 3602.314 kb on + strand, within LRK53_RS16365 at 3602.315 kb on - strand, within LRK53_RS16365 at 3602.315 kb on - strand, within LRK53_RS16365 at 3602.315 kb on - strand, within LRK53_RS16365 at 3602.315 kb on - strand, within LRK53_RS16365 at 3602.315 kb on - strand, within LRK53_RS16365 at 3602.416 kb on + strand, within LRK53_RS16365 at 3602.417 kb on - strand, within LRK53_RS16365
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 3,600,028 + -0.8 3,600,028 + +0.0 3,600,028 + -0.4 3,600,029 - -0.8 3,600,029 - -0.5 3,600,029 - +0.0 3,600,029 - -0.4 3,600,124 - -1.6 3,600,124 - -1.1 3,600,126 + +0.3 3,600,126 + +0.4 3,600,126 + -1.0 3,600,127 - -1.0 3,600,127 - -0.8 3,600,127 - -1.0 3,600,127 - -0.3 3,600,240 + -0.8 3,600,241 - -1.1 3,600,241 - -1.6 3,600,241 - -1.3 3,600,241 - +2.0 3,600,393 + -0.3 3,600,529 + +1.6 3,600,529 + -0.6 3,600,715 + LRK53_RS16355 0.77 -2.1 3,600,749 - -1.0 3,600,749 - -1.1 3,600,762 + -1.7 3,600,763 - -0.6 3,600,763 - -0.7 3,600,802 + +1.0 3,600,850 + +1.8 3,600,850 + +0.4 3,600,851 - -1.6 3,600,851 - -0.8 3,601,042 + LRK53_RS16360 0.14 +1.0 3,601,042 + LRK53_RS16360 0.14 -2.7 3,601,042 + LRK53_RS16360 0.14 -0.6 3,601,043 - LRK53_RS16360 0.14 -1.6 3,601,043 - LRK53_RS16360 0.14 -0.3 3,601,043 - LRK53_RS16360 0.14 -1.3 3,601,043 - LRK53_RS16360 0.14 -0.3 3,601,043 - LRK53_RS16360 0.14 -1.7 3,601,063 + LRK53_RS16360 0.19 -2.3 3,601,063 + LRK53_RS16360 0.19 +0.0 3,601,063 + LRK53_RS16360 0.19 +0.6 3,601,064 - LRK53_RS16360 0.19 -2.2 3,601,064 - LRK53_RS16360 0.19 -0.6 3,601,064 - LRK53_RS16360 0.19 +0.0 3,601,064 - LRK53_RS16360 0.19 -1.6 3,601,064 - LRK53_RS16360 0.19 +0.0 3,601,104 + LRK53_RS16360 0.28 -0.6 3,601,104 + LRK53_RS16360 0.28 -1.3 3,601,105 - LRK53_RS16360 0.28 -0.8 3,601,117 + LRK53_RS16360 0.31 +0.0 3,601,117 + LRK53_RS16360 0.31 -1.1 3,601,118 - LRK53_RS16360 0.31 -1.2 3,601,118 - LRK53_RS16360 0.31 +0.0 3,601,118 - LRK53_RS16360 0.31 -1.3 3,601,121 + LRK53_RS16360 0.32 -0.6 3,601,121 + LRK53_RS16360 0.32 -1.8 3,601,121 + LRK53_RS16360 0.32 +0.4 3,601,121 + LRK53_RS16360 0.32 +0.0 3,601,121 + LRK53_RS16360 0.32 -1.4 3,601,122 - LRK53_RS16360 0.32 -1.0 3,601,122 - LRK53_RS16360 0.32 -1.1 3,601,122 - LRK53_RS16360 0.32 -2.1 3,601,122 - LRK53_RS16360 0.32 -0.7 3,601,122 - LRK53_RS16360 0.32 -1.0 3,601,122 - LRK53_RS16360 0.32 +0.8 3,601,156 + LRK53_RS16360 0.40 -1.4 3,601,156 + LRK53_RS16360 0.40 +1.0 3,601,157 - LRK53_RS16360 0.40 -1.0 3,601,157 - LRK53_RS16360 0.40 +0.0 3,601,157 - LRK53_RS16360 0.40 -0.9 3,601,157 - LRK53_RS16360 0.40 -1.3 3,601,157 - LRK53_RS16360 0.40 +0.0 3,601,157 - LRK53_RS16360 0.40 -1.0 3,601,157 - LRK53_RS16360 0.40 +1.0 3,601,157 - LRK53_RS16360 0.40 +0.6 3,601,218 + LRK53_RS16360 0.54 +0.0 3,601,219 - LRK53_RS16360 0.54 +1.0 3,601,219 - LRK53_RS16360 0.54 +0.9 3,601,219 - LRK53_RS16360 0.54 -0.4 3,601,219 - LRK53_RS16360 0.54 -0.7 3,601,219 - LRK53_RS16360 0.54 -1.4 3,601,219 - LRK53_RS16360 0.54 -1.4 3,601,219 - LRK53_RS16360 0.54 -0.6 3,601,219 - LRK53_RS16360 0.54 -1.3 3,601,219 - LRK53_RS16360 0.54 -0.6 3,601,257 + LRK53_RS16360 0.63 +0.0 3,601,257 + LRK53_RS16360 0.63 -1.5 3,601,257 + LRK53_RS16360 0.63 +0.4 3,601,257 + LRK53_RS16360 0.63 -0.2 3,601,258 - LRK53_RS16360 0.63 -1.3 3,601,258 - LRK53_RS16360 0.63 -1.2 3,601,461 + -1.1 3,601,478 + -2.3 3,601,478 + -0.3 3,601,478 + -0.6 3,601,479 - -1.4 3,601,479 - -1.0 3,601,486 + -0.7 3,601,487 - -1.1 3,601,600 + -0.9 3,601,600 + -0.6 3,601,600 + +1.0 3,601,600 + +1.0 3,601,601 - -0.3 3,601,601 - +0.4 3,601,601 - -0.6 3,601,627 + LRK53_RS16365 0.11 -0.5 3,601,628 - LRK53_RS16365 0.11 +1.0 3,601,628 - LRK53_RS16365 0.11 -0.3 3,601,783 + LRK53_RS16365 0.19 -1.0 3,601,783 + LRK53_RS16365 0.19 +0.0 3,601,783 + LRK53_RS16365 0.19 -0.6 3,601,783 + LRK53_RS16365 0.19 -0.7 3,601,811 - LRK53_RS16365 0.21 -0.6 3,601,811 - LRK53_RS16365 0.21 -1.4 3,601,874 + LRK53_RS16365 0.24 -0.3 3,601,875 - LRK53_RS16365 0.24 +0.4 3,601,875 - LRK53_RS16365 0.24 -1.3 3,601,875 - LRK53_RS16365 0.24 -0.1 3,601,888 + LRK53_RS16365 0.25 -0.6 3,601,888 + LRK53_RS16365 0.25 -1.4 3,601,888 + LRK53_RS16365 0.25 -2.0 3,601,889 - LRK53_RS16365 0.25 +0.0 3,601,889 - LRK53_RS16365 0.25 -1.0 3,602,150 - LRK53_RS16365 0.39 -2.3 3,602,314 + LRK53_RS16365 0.48 -1.6 3,602,314 + LRK53_RS16365 0.48 +0.0 3,602,315 - LRK53_RS16365 0.48 +0.0 3,602,315 - LRK53_RS16365 0.48 +1.4 3,602,315 - LRK53_RS16365 0.48 -0.9 3,602,315 - LRK53_RS16365 0.48 +1.6 3,602,315 - LRK53_RS16365 0.48 -1.5 3,602,416 + LRK53_RS16365 0.53 -1.0 3,602,417 - LRK53_RS16365 0.53 -0.6
Or see this region's nucleotide sequence