Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09520 and LRK53_RS09525 are separated by 132 nucleotides LRK53_RS09525 and LRK53_RS09530 are separated by 57 nucleotides LRK53_RS09530 and LRK53_RS09535 are separated by 168 nucleotides
LRK53_RS09520: LRK53_RS09520 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 2,142,987 to 2,143,955
_RS09520
LRK53_RS09525: LRK53_RS09525 - RNA chaperone Hfq, at 2,144,088 to 2,144,384
_RS09525
LRK53_RS09530: LRK53_RS09530 - GTPase HflX, at 2,144,442 to 2,145,743
_RS09530
LRK53_RS09535: LRK53_RS09535 - FtsH protease activity modulator HflK, at 2,145,912 to 2,146,982
_RS09535
Position (kb)
2144
2145
2146 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.910 kb on - strand at 2143.997 kb on + strand at 2143.997 kb on + strand at 2143.998 kb on - strand at 2144.011 kb on + strand at 2144.011 kb on + strand at 2144.012 kb on - strand at 2144.012 kb on - strand at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.385 kb on + strand at 2144.385 kb on + strand at 2144.386 kb on - strand at 2144.386 kb on - strand at 2144.386 kb on - strand at 2144.493 kb on - strand at 2144.493 kb on - strand at 2144.510 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.567 kb on - strand at 2144.610 kb on + strand, within LRK53_RS09530 at 2144.610 kb on + strand, within LRK53_RS09530 at 2144.611 kb on - strand, within LRK53_RS09530 at 2144.611 kb on - strand, within LRK53_RS09530 at 2144.611 kb on - strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.854 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2145.060 kb on + strand, within LRK53_RS09530 at 2145.061 kb on - strand, within LRK53_RS09530 at 2145.061 kb on - strand, within LRK53_RS09530 at 2145.114 kb on + strand, within LRK53_RS09530 at 2145.114 kb on + strand, within LRK53_RS09530 at 2145.724 kb on + strand at 2145.724 kb on + strand at 2145.724 kb on + strand at 2145.724 kb on + strand at 2145.724 kb on + strand at 2145.724 kb on + strand at 2145.725 kb on - strand at 2145.725 kb on - strand at 2145.725 kb on - strand at 2145.725 kb on - strand at 2145.769 kb on + strand at 2145.770 kb on - strand at 2145.770 kb on - strand at 2145.770 kb on - strand at 2145.770 kb on - strand at 2145.770 kb on - strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.843 kb on + strand at 2145.844 kb on - strand at 2145.844 kb on - strand at 2145.844 kb on - strand at 2145.844 kb on - strand at 2145.844 kb on - strand at 2145.844 kb on - strand at 2145.844 kb on - strand at 2145.898 kb on + strand at 2145.898 kb on + strand at 2145.898 kb on + strand at 2145.899 kb on - strand at 2146.021 kb on + strand, within LRK53_RS09535 at 2146.021 kb on + strand, within LRK53_RS09535 at 2146.022 kb on - strand, within LRK53_RS09535 at 2146.022 kb on - strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.101 kb on + strand, within LRK53_RS09535 at 2146.102 kb on - strand, within LRK53_RS09535 at 2146.102 kb on - strand, within LRK53_RS09535 at 2146.102 kb on - strand, within LRK53_RS09535 at 2146.102 kb on - strand, within LRK53_RS09535 at 2146.102 kb on - strand, within LRK53_RS09535 at 2146.152 kb on + strand, within LRK53_RS09535 at 2146.152 kb on + strand, within LRK53_RS09535 at 2146.308 kb on + strand, within LRK53_RS09535 at 2146.308 kb on + strand, within LRK53_RS09535 at 2146.308 kb on + strand, within LRK53_RS09535 at 2146.308 kb on + strand, within LRK53_RS09535 at 2146.308 kb on + strand, within LRK53_RS09535 at 2146.309 kb on - strand, within LRK53_RS09535 at 2146.309 kb on - strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.332 kb on + strand, within LRK53_RS09535 at 2146.333 kb on - strand, within LRK53_RS09535 at 2146.333 kb on - strand, within LRK53_RS09535 at 2146.333 kb on - strand, within LRK53_RS09535 at 2146.494 kb on + strand, within LRK53_RS09535 at 2146.494 kb on + strand, within LRK53_RS09535 at 2146.494 kb on + strand, within LRK53_RS09535 at 2146.494 kb on + strand, within LRK53_RS09535 at 2146.494 kb on + strand, within LRK53_RS09535 at 2146.494 kb on + strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.495 kb on - strand, within LRK53_RS09535 at 2146.627 kb on - strand, within LRK53_RS09535 at 2146.627 kb on - strand, within LRK53_RS09535 at 2146.692 kb on + strand, within LRK53_RS09535 at 2146.692 kb on + strand, within LRK53_RS09535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 2,143,909 + -1.5 2,143,909 + -0.2 2,143,909 + +0.2 2,143,909 + -1.7 2,143,909 + -1.9 2,143,909 + -2.1 2,143,910 - -0.7 2,143,997 + -1.2 2,143,997 + -1.0 2,143,998 - -0.1 2,144,011 + -0.1 2,144,011 + -1.7 2,144,012 - -0.9 2,144,012 - +1.9 2,144,290 + LRK53_RS09525 0.68 -0.1 2,144,290 + LRK53_RS09525 0.68 -1.2 2,144,290 + LRK53_RS09525 0.68 +1.9 2,144,290 + LRK53_RS09525 0.68 -0.1 2,144,290 + LRK53_RS09525 0.68 -1.1 2,144,385 + -1.1 2,144,385 + -1.1 2,144,386 - -1.2 2,144,386 - -0.1 2,144,386 - -1.2 2,144,493 - -1.4 2,144,493 - -1.1 2,144,510 + -1.0 2,144,566 + -0.1 2,144,566 + -0.1 2,144,566 + -2.1 2,144,566 + -2.1 2,144,566 + -1.9 2,144,567 - -0.5 2,144,610 + LRK53_RS09530 0.13 -0.9 2,144,610 + LRK53_RS09530 0.13 -0.7 2,144,611 - LRK53_RS09530 0.13 -3.0 2,144,611 - LRK53_RS09530 0.13 -1.0 2,144,611 - LRK53_RS09530 0.13 -1.8 2,144,613 + LRK53_RS09530 0.13 -1.1 2,144,613 + LRK53_RS09530 0.13 -0.8 2,144,613 + LRK53_RS09530 0.13 +0.9 2,144,613 + LRK53_RS09530 0.13 -1.5 2,144,613 + LRK53_RS09530 0.13 -1.5 2,144,613 + LRK53_RS09530 0.13 -1.7 2,144,613 + LRK53_RS09530 0.13 -1.4 2,144,613 + LRK53_RS09530 0.13 -0.7 2,144,613 + LRK53_RS09530 0.13 -0.7 2,144,613 + LRK53_RS09530 0.13 -0.3 2,144,613 + LRK53_RS09530 0.13 -0.1 2,144,613 + LRK53_RS09530 0.13 +0.1 2,144,613 + LRK53_RS09530 0.13 +0.9 2,144,613 + LRK53_RS09530 0.13 -1.1 2,144,613 + LRK53_RS09530 0.13 -0.7 2,144,613 + LRK53_RS09530 0.13 -2.7 2,144,613 + LRK53_RS09530 0.13 -1.5 2,144,613 + LRK53_RS09530 0.13 +0.9 2,144,613 + LRK53_RS09530 0.13 -1.1 2,144,613 + LRK53_RS09530 0.13 -1.7 2,144,613 + LRK53_RS09530 0.13 -1.4 2,144,613 + LRK53_RS09530 0.13 -0.4 2,144,613 + LRK53_RS09530 0.13 -1.1 2,144,613 + LRK53_RS09530 0.13 +0.0 2,144,613 + LRK53_RS09530 0.13 -0.1 2,144,614 - LRK53_RS09530 0.13 -2.1 2,144,614 - LRK53_RS09530 0.13 -1.1 2,144,614 - LRK53_RS09530 0.13 -1.1 2,144,614 - LRK53_RS09530 0.13 -2.1 2,144,614 - LRK53_RS09530 0.13 -0.1 2,144,614 - LRK53_RS09530 0.13 -0.1 2,144,614 - LRK53_RS09530 0.13 +0.3 2,144,614 - LRK53_RS09530 0.13 +0.9 2,144,614 - LRK53_RS09530 0.13 -2.0 2,144,614 - LRK53_RS09530 0.13 -1.4 2,144,614 - LRK53_RS09530 0.13 +0.6 2,144,854 + LRK53_RS09530 0.32 -1.7 2,144,989 + LRK53_RS09530 0.42 -0.7 2,144,989 + LRK53_RS09530 0.42 +0.3 2,144,989 + LRK53_RS09530 0.42 -0.1 2,144,989 + LRK53_RS09530 0.42 -0.1 2,144,989 + LRK53_RS09530 0.42 -0.7 2,144,989 + LRK53_RS09530 0.42 -1.8 2,144,989 + LRK53_RS09530 0.42 -0.1 2,144,989 + LRK53_RS09530 0.42 -0.7 2,144,989 + LRK53_RS09530 0.42 -1.1 2,144,989 + LRK53_RS09530 0.42 -1.1 2,144,989 + LRK53_RS09530 0.42 -2.3 2,144,989 + LRK53_RS09530 0.42 -0.4 2,144,989 + LRK53_RS09530 0.42 -2.1 2,144,990 - LRK53_RS09530 0.42 -1.9 2,144,990 - LRK53_RS09530 0.42 -0.4 2,144,990 - LRK53_RS09530 0.42 -1.1 2,144,990 - LRK53_RS09530 0.42 -1.1 2,144,990 - LRK53_RS09530 0.42 -0.3 2,145,060 + LRK53_RS09530 0.47 -1.9 2,145,061 - LRK53_RS09530 0.48 +0.3 2,145,061 - LRK53_RS09530 0.48 -1.0 2,145,114 + LRK53_RS09530 0.52 -1.2 2,145,114 + LRK53_RS09530 0.52 -0.1 2,145,724 + -0.7 2,145,724 + +0.9 2,145,724 + +0.5 2,145,724 + -0.6 2,145,724 + +1.2 2,145,724 + +0.0 2,145,725 - -0.7 2,145,725 - -0.3 2,145,725 - -0.1 2,145,725 - -0.4 2,145,769 + -0.9 2,145,770 - -0.1 2,145,770 - -0.1 2,145,770 - -1.2 2,145,770 - -0.7 2,145,770 - -0.1 2,145,843 + -0.9 2,145,843 + +1.5 2,145,843 + -0.7 2,145,843 + -1.1 2,145,843 + -1.1 2,145,843 + +0.7 2,145,843 + -0.1 2,145,843 + +0.9 2,145,843 + -1.7 2,145,843 + -1.2 2,145,843 + -0.6 2,145,843 + -1.7 2,145,843 + +0.9 2,145,844 - -1.7 2,145,844 - -0.1 2,145,844 - -0.2 2,145,844 - -1.8 2,145,844 - -0.3 2,145,844 - -2.1 2,145,844 - -1.4 2,145,898 + -0.7 2,145,898 + -2.1 2,145,898 + -1.2 2,145,899 - -1.1 2,146,021 + LRK53_RS09535 0.10 -0.7 2,146,021 + LRK53_RS09535 0.10 -0.4 2,146,022 - LRK53_RS09535 0.10 -1.7 2,146,022 - LRK53_RS09535 0.10 -2.2 2,146,101 + LRK53_RS09535 0.18 -1.0 2,146,101 + LRK53_RS09535 0.18 -1.5 2,146,101 + LRK53_RS09535 0.18 -0.4 2,146,101 + LRK53_RS09535 0.18 -1.1 2,146,101 + LRK53_RS09535 0.18 -3.2 2,146,101 + LRK53_RS09535 0.18 +0.3 2,146,101 + LRK53_RS09535 0.18 -1.8 2,146,101 + LRK53_RS09535 0.18 +0.3 2,146,101 + LRK53_RS09535 0.18 -0.4 2,146,101 + LRK53_RS09535 0.18 -0.7 2,146,101 + LRK53_RS09535 0.18 -1.1 2,146,101 + LRK53_RS09535 0.18 -0.7 2,146,101 + LRK53_RS09535 0.18 -2.1 2,146,102 - LRK53_RS09535 0.18 -1.9 2,146,102 - LRK53_RS09535 0.18 -0.7 2,146,102 - LRK53_RS09535 0.18 -1.1 2,146,102 - LRK53_RS09535 0.18 -1.1 2,146,102 - LRK53_RS09535 0.18 -1.1 2,146,152 + LRK53_RS09535 0.22 -0.8 2,146,152 + LRK53_RS09535 0.22 -1.4 2,146,308 + LRK53_RS09535 0.37 -0.3 2,146,308 + LRK53_RS09535 0.37 -1.8 2,146,308 + LRK53_RS09535 0.37 -1.8 2,146,308 + LRK53_RS09535 0.37 -1.7 2,146,308 + LRK53_RS09535 0.37 -0.1 2,146,309 - LRK53_RS09535 0.37 -0.7 2,146,309 - LRK53_RS09535 0.37 -1.2 2,146,332 + LRK53_RS09535 0.39 -0.7 2,146,332 + LRK53_RS09535 0.39 -0.9 2,146,332 + LRK53_RS09535 0.39 -2.4 2,146,332 + LRK53_RS09535 0.39 -1.5 2,146,332 + LRK53_RS09535 0.39 -1.5 2,146,332 + LRK53_RS09535 0.39 -1.7 2,146,332 + LRK53_RS09535 0.39 -4.1 2,146,332 + LRK53_RS09535 0.39 -3.4 2,146,332 + LRK53_RS09535 0.39 +0.5 2,146,332 + LRK53_RS09535 0.39 -0.7 2,146,332 + LRK53_RS09535 0.39 -2.2 2,146,332 + LRK53_RS09535 0.39 +0.9 2,146,333 - LRK53_RS09535 0.39 +1.9 2,146,333 - LRK53_RS09535 0.39 +0.3 2,146,333 - LRK53_RS09535 0.39 -0.1 2,146,494 + LRK53_RS09535 0.54 -2.4 2,146,494 + LRK53_RS09535 0.54 -1.4 2,146,494 + LRK53_RS09535 0.54 -1.0 2,146,494 + LRK53_RS09535 0.54 -0.4 2,146,494 + LRK53_RS09535 0.54 -0.7 2,146,494 + LRK53_RS09535 0.54 -1.1 2,146,495 - LRK53_RS09535 0.54 -1.7 2,146,495 - LRK53_RS09535 0.54 -1.2 2,146,495 - LRK53_RS09535 0.54 -0.9 2,146,495 - LRK53_RS09535 0.54 -1.9 2,146,495 - LRK53_RS09535 0.54 -0.7 2,146,495 - LRK53_RS09535 0.54 -1.7 2,146,495 - LRK53_RS09535 0.54 -1.4 2,146,495 - LRK53_RS09535 0.54 -1.4 2,146,627 - LRK53_RS09535 0.67 -0.7 2,146,627 - LRK53_RS09535 0.67 +1.9 2,146,692 + LRK53_RS09535 0.73 -0.1 2,146,692 + LRK53_RS09535 0.73 -2.0
Or see this region's nucleotide sequence