Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04185 and LRK53_RS04190 are separated by 15 nucleotides LRK53_RS04190 and LRK53_RS04195 are separated by 49 nucleotides LRK53_RS04195 and LRK53_RS04200 are separated by 78 nucleotides
LRK53_RS04185: LRK53_RS04185 - dihydrolipoyllysine-residue acetyltransferase, at 978,276 to 979,691
_RS04185
LRK53_RS04190: LRK53_RS04190 - dihydrolipoyl dehydrogenase, at 979,707 to 981,521
_RS04190
LRK53_RS04195: LRK53_RS04195 - 4-hydroxy-tetrahydrodipicolinate reductase, at 981,571 to 982,371
_RS04195
LRK53_RS04200: LRK53_RS04200 - hypothetical protein, at 982,450 to 982,926
_RS04200
Position (kb) 979
980
981
982 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 978.786 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.894 kb on + strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 978.895 kb on - strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.476 kb on + strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.477 kb on - strand, within LRK53_RS04185 at 979.599 kb on + strand at 979.600 kb on - strand at 979.600 kb on - strand at 980.157 kb on + strand, within LRK53_RS04190 at 980.214 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.682 kb on + strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.683 kb on - strand, within LRK53_RS04190 at 980.860 kb on - strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.027 kb on + strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.028 kb on - strand, within LRK53_RS04190 at 981.477 kb on + strand at 981.477 kb on + strand at 981.477 kb on + strand at 981.477 kb on + strand at 981.478 kb on - strand at 981.478 kb on - strand at 981.501 kb on + strand at 981.501 kb on + strand at 981.501 kb on + strand at 981.501 kb on + strand at 981.502 kb on - strand at 981.502 kb on - strand at 981.502 kb on - strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.584 kb on + strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.585 kb on - strand at 981.739 kb on + strand, within LRK53_RS04195 at 981.739 kb on + strand, within LRK53_RS04195 at 981.739 kb on + strand, within LRK53_RS04195 at 981.740 kb on - strand, within LRK53_RS04195 at 981.740 kb on - strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.779 kb on + strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.780 kb on - strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.790 kb on + strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.791 kb on - strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.886 kb on + strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 981.887 kb on - strand, within LRK53_RS04195 at 982.025 kb on - strand, within LRK53_RS04195 at 982.025 kb on - strand, within LRK53_RS04195 at 982.285 kb on + strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.286 kb on - strand, within LRK53_RS04195 at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.369 kb on + strand at 982.370 kb on - strand at 982.370 kb on - strand at 982.370 kb on - strand at 982.370 kb on - strand at 982.451 kb on + strand at 982.451 kb on + strand at 982.451 kb on + strand at 982.451 kb on + strand at 982.452 kb on - strand at 982.452 kb on - strand at 982.452 kb on - strand at 982.452 kb on - strand at 982.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 978,786 + LRK53_RS04185 0.36 -0.0 978,894 + LRK53_RS04185 0.44 -2.2 978,894 + LRK53_RS04185 0.44 -1.0 978,894 + LRK53_RS04185 0.44 -1.6 978,894 + LRK53_RS04185 0.44 -1.6 978,894 + LRK53_RS04185 0.44 -1.0 978,894 + LRK53_RS04185 0.44 -1.0 978,894 + LRK53_RS04185 0.44 +1.0 978,894 + LRK53_RS04185 0.44 -0.0 978,894 + LRK53_RS04185 0.44 -1.8 978,895 - LRK53_RS04185 0.44 -1.4 978,895 - LRK53_RS04185 0.44 +1.0 978,895 - LRK53_RS04185 0.44 -2.0 978,895 - LRK53_RS04185 0.44 -2.0 978,895 - LRK53_RS04185 0.44 -0.0 978,895 - LRK53_RS04185 0.44 +0.4 979,476 + LRK53_RS04185 0.85 -0.0 979,476 + LRK53_RS04185 0.85 -1.9 979,476 + LRK53_RS04185 0.85 +1.0 979,476 + LRK53_RS04185 0.85 -2.6 979,476 + LRK53_RS04185 0.85 -2.0 979,477 - LRK53_RS04185 0.85 +1.0 979,477 - LRK53_RS04185 0.85 -0.6 979,477 - LRK53_RS04185 0.85 -0.0 979,599 + -2.0 979,600 - -0.0 979,600 - -2.7 980,157 + LRK53_RS04190 0.25 -1.5 980,214 + LRK53_RS04190 0.28 -1.6 980,682 + LRK53_RS04190 0.54 -1.0 980,682 + LRK53_RS04190 0.54 -1.4 980,682 + LRK53_RS04190 0.54 -0.0 980,683 - LRK53_RS04190 0.54 -2.2 980,683 - LRK53_RS04190 0.54 -1.0 980,683 - LRK53_RS04190 0.54 +1.0 980,860 - LRK53_RS04190 0.64 +2.0 981,027 + LRK53_RS04190 0.73 -1.0 981,027 + LRK53_RS04190 0.73 -1.9 981,027 + LRK53_RS04190 0.73 -1.6 981,027 + LRK53_RS04190 0.73 -0.0 981,027 + LRK53_RS04190 0.73 -1.6 981,027 + LRK53_RS04190 0.73 -2.7 981,027 + LRK53_RS04190 0.73 -0.6 981,027 + LRK53_RS04190 0.73 -1.9 981,027 + LRK53_RS04190 0.73 -0.0 981,027 + LRK53_RS04190 0.73 -1.0 981,027 + LRK53_RS04190 0.73 -0.6 981,027 + LRK53_RS04190 0.73 -0.0 981,028 - LRK53_RS04190 0.73 -0.0 981,028 - LRK53_RS04190 0.73 -0.6 981,028 - LRK53_RS04190 0.73 -2.9 981,028 - LRK53_RS04190 0.73 -0.0 981,028 - LRK53_RS04190 0.73 -0.6 981,028 - LRK53_RS04190 0.73 -1.0 981,028 - LRK53_RS04190 0.73 -3.1 981,028 - LRK53_RS04190 0.73 -1.9 981,028 - LRK53_RS04190 0.73 -0.0 981,028 - LRK53_RS04190 0.73 -0.6 981,028 - LRK53_RS04190 0.73 -1.0 981,028 - LRK53_RS04190 0.73 -2.4 981,028 - LRK53_RS04190 0.73 -1.4 981,028 - LRK53_RS04190 0.73 -1.9 981,028 - LRK53_RS04190 0.73 -1.6 981,028 - LRK53_RS04190 0.73 +0.4 981,477 + -1.0 981,477 + -0.6 981,477 + -1.9 981,477 + -0.4 981,478 - -1.2 981,478 - -1.4 981,501 + -1.0 981,501 + -0.0 981,501 + -0.0 981,501 + -0.2 981,502 - -0.6 981,502 - -1.1 981,502 - +0.4 981,584 + -1.4 981,584 + -0.0 981,584 + -0.8 981,584 + +0.5 981,584 + -0.7 981,584 + -1.0 981,584 + -1.9 981,584 + -0.0 981,584 + -1.3 981,584 + -0.0 981,584 + -2.2 981,584 + -0.3 981,584 + -2.0 981,584 + -1.5 981,584 + -1.2 981,584 + -1.6 981,584 + +1.0 981,584 + -1.0 981,584 + -0.6 981,584 + -2.2 981,584 + -1.4 981,584 + -0.6 981,584 + -1.4 981,584 + -0.7 981,584 + -1.4 981,584 + +1.0 981,584 + -0.9 981,584 + -0.6 981,584 + -0.4 981,584 + +1.0 981,584 + -1.6 981,584 + -1.2 981,584 + -0.0 981,584 + -0.1 981,584 + -0.6 981,584 + +1.0 981,584 + +1.0 981,584 + -1.6 981,584 + -0.1 981,584 + -1.4 981,584 + +0.2 981,585 - -0.5 981,585 - +2.5 981,585 - -0.0 981,585 - +0.2 981,585 - -0.5 981,585 - +0.4 981,585 - -0.6 981,585 - -0.6 981,585 - +1.0 981,585 - -1.4 981,585 - -2.0 981,585 - -2.0 981,585 - -0.5 981,585 - -0.6 981,585 - -0.1 981,585 - -0.6 981,585 - -1.0 981,585 - -0.6 981,585 - -0.0 981,585 - -0.6 981,585 - -1.0 981,585 - -1.4 981,585 - -1.9 981,585 - -2.1 981,585 - +0.4 981,585 - -1.0 981,585 - -2.2 981,585 - -0.4 981,585 - -1.2 981,585 - -1.6 981,585 - -1.0 981,739 + LRK53_RS04195 0.21 -0.4 981,739 + LRK53_RS04195 0.21 +0.1 981,739 + LRK53_RS04195 0.21 -1.1 981,740 - LRK53_RS04195 0.21 -2.0 981,740 - LRK53_RS04195 0.21 -1.0 981,779 + LRK53_RS04195 0.26 -1.0 981,779 + LRK53_RS04195 0.26 -0.0 981,779 + LRK53_RS04195 0.26 +2.5 981,779 + LRK53_RS04195 0.26 -0.4 981,779 + LRK53_RS04195 0.26 +1.0 981,779 + LRK53_RS04195 0.26 -0.0 981,779 + LRK53_RS04195 0.26 -0.0 981,779 + LRK53_RS04195 0.26 -0.8 981,779 + LRK53_RS04195 0.26 +0.2 981,779 + LRK53_RS04195 0.26 +0.4 981,779 + LRK53_RS04195 0.26 -1.9 981,779 + LRK53_RS04195 0.26 -1.6 981,779 + LRK53_RS04195 0.26 -0.8 981,779 + LRK53_RS04195 0.26 -1.1 981,779 + LRK53_RS04195 0.26 -0.6 981,779 + LRK53_RS04195 0.26 -0.8 981,779 + LRK53_RS04195 0.26 -1.0 981,779 + LRK53_RS04195 0.26 -1.4 981,780 - LRK53_RS04195 0.26 -1.4 981,780 - LRK53_RS04195 0.26 -1.0 981,780 - LRK53_RS04195 0.26 -1.4 981,780 - LRK53_RS04195 0.26 -0.6 981,780 - LRK53_RS04195 0.26 -0.5 981,780 - LRK53_RS04195 0.26 -1.2 981,780 - LRK53_RS04195 0.26 -1.0 981,780 - LRK53_RS04195 0.26 -1.9 981,780 - LRK53_RS04195 0.26 -1.7 981,780 - LRK53_RS04195 0.26 +1.0 981,780 - LRK53_RS04195 0.26 -0.4 981,780 - LRK53_RS04195 0.26 -0.2 981,780 - LRK53_RS04195 0.26 -0.8 981,780 - LRK53_RS04195 0.26 +0.4 981,780 - LRK53_RS04195 0.26 +0.4 981,780 - LRK53_RS04195 0.26 -1.0 981,780 - LRK53_RS04195 0.26 -1.9 981,790 + LRK53_RS04195 0.27 -1.5 981,790 + LRK53_RS04195 0.27 -2.2 981,790 + LRK53_RS04195 0.27 -0.2 981,790 + LRK53_RS04195 0.27 -1.4 981,790 + LRK53_RS04195 0.27 -0.0 981,790 + LRK53_RS04195 0.27 -0.2 981,790 + LRK53_RS04195 0.27 +1.0 981,790 + LRK53_RS04195 0.27 -0.3 981,790 + LRK53_RS04195 0.27 -0.4 981,791 - LRK53_RS04195 0.27 -0.6 981,791 - LRK53_RS04195 0.27 -0.0 981,791 - LRK53_RS04195 0.27 -0.7 981,791 - LRK53_RS04195 0.27 +1.0 981,791 - LRK53_RS04195 0.27 -1.0 981,791 - LRK53_RS04195 0.27 -0.0 981,791 - LRK53_RS04195 0.27 -2.2 981,791 - LRK53_RS04195 0.27 +1.0 981,886 + LRK53_RS04195 0.39 +1.3 981,886 + LRK53_RS04195 0.39 -1.9 981,886 + LRK53_RS04195 0.39 -1.9 981,886 + LRK53_RS04195 0.39 -0.4 981,886 + LRK53_RS04195 0.39 -0.5 981,886 + LRK53_RS04195 0.39 -3.2 981,887 - LRK53_RS04195 0.39 -0.6 981,887 - LRK53_RS04195 0.39 -2.7 981,887 - LRK53_RS04195 0.39 -0.0 981,887 - LRK53_RS04195 0.39 -1.4 981,887 - LRK53_RS04195 0.39 -0.0 981,887 - LRK53_RS04195 0.39 -0.8 981,887 - LRK53_RS04195 0.39 +0.2 981,887 - LRK53_RS04195 0.39 -0.6 981,887 - LRK53_RS04195 0.39 -0.6 982,025 - LRK53_RS04195 0.57 -0.5 982,025 - LRK53_RS04195 0.57 +2.0 982,285 + LRK53_RS04195 0.89 +0.2 982,286 - LRK53_RS04195 0.89 -1.0 982,286 - LRK53_RS04195 0.89 +1.0 982,286 - LRK53_RS04195 0.89 -0.0 982,286 - LRK53_RS04195 0.89 -0.6 982,286 - LRK53_RS04195 0.89 -0.6 982,369 + -0.5 982,369 + -1.4 982,369 + +1.0 982,369 + -0.0 982,369 + -1.0 982,369 + +1.0 982,370 - -0.4 982,370 - +1.0 982,370 - +0.4 982,370 - +1.5 982,451 + -0.0 982,451 + -0.9 982,451 + -1.1 982,451 + -1.4 982,452 - -0.4 982,452 - +1.0 982,452 - -1.5 982,452 - -0.8 982,452 - +0.6
Or see this region's nucleotide sequence