Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28525

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaA and wzy overlap by 4 nucleotideswzy and wcaA overlap by 32 nucleotideswcaA and wzx overlap by 14 nucleotides JDDGAC_28520: wcaA - glycosyl transferase, at 5,471,962 to 5,472,744 wcaA JDDGAC_28525: wzy - O157 family O-antigen polymerase, at 5,472,741 to 5,473,925 wzy JDDGAC_28530: wcaA - glycosyl transferase, at 5,473,894 to 5,474,640 wcaA JDDGAC_28535: wzx - O157 family O-antigen flippase, at 5,474,627 to 5,476,018 wzx Position (kb) 5472 5473 5474Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7 8 9at 5472.302 kb on + strand, within wcaAat 5472.635 kb on + strand, within wcaAat 5472.676 kb on + strandat 5472.786 kb on + strandat 5473.094 kb on + strand, within wzyat 5473.120 kb on + strand, within wzyat 5473.336 kb on + strand, within wzyat 5473.336 kb on + strand, within wzyat 5473.841 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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5,472,302 + wcaA JDDGAC_28520 0.43 -3.6
5,472,635 + wcaA JDDGAC_28520 0.86 -2.7
5,472,676 + -1.5
5,472,786 + +9.0
5,473,094 + wzy JDDGAC_28525 0.30 +8.7
5,473,120 + wzy JDDGAC_28525 0.32 +8.2
5,473,336 + wzy JDDGAC_28525 0.50 +8.9
5,473,336 + wzy JDDGAC_28525 0.50 +9.3
5,473,841 - +8.1

Or see this region's nucleotide sequence