Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18930 and LRK53_RS18935 overlap by 1 nucleotides LRK53_RS18935 and LRK53_RS18940 are separated by 121 nucleotides LRK53_RS18940 and LRK53_RS18945 are separated by 11 nucleotides LRK53_RS18945 and LRK53_RS18950 are separated by 300 nucleotides
LRK53_RS18930: LRK53_RS18930 - hypothetical protein, at 181,747 to 182,415
_RS18930
LRK53_RS18935: LRK53_RS18935 - PD-(D/E)XK nuclease family protein, at 182,415 to 183,017
_RS18935
LRK53_RS18940: LRK53_RS18940 - hypothetical protein, at 183,139 to 183,600
_RS18940
LRK53_RS18945: LRK53_RS18945 - hypothetical protein, at 183,612 to 183,833
_RS18945
LRK53_RS18950: LRK53_RS18950 - hypothetical protein, at 184,134 to 184,583
_RS18950
Position (kb) 183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 182.237 kb on - strand, within LRK53_RS18930 at 182.237 kb on - strand, within LRK53_RS18930 at 182.237 kb on - strand, within LRK53_RS18930 at 182.349 kb on + strand at 182.349 kb on + strand at 182.460 kb on + strand at 182.460 kb on + strand at 182.460 kb on + strand at 182.460 kb on + strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.461 kb on - strand at 182.520 kb on + strand, within LRK53_RS18935 at 182.521 kb on - strand, within LRK53_RS18935 at 182.521 kb on - strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.589 kb on + strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.590 kb on - strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.664 kb on + strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.665 kb on - strand, within LRK53_RS18935 at 182.709 kb on + strand, within LRK53_RS18935 at 182.798 kb on + strand, within LRK53_RS18935 at 182.799 kb on - strand, within LRK53_RS18935 at 182.799 kb on - strand, within LRK53_RS18935 at 182.799 kb on - strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.829 kb on + strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.830 kb on - strand, within LRK53_RS18935 at 182.883 kb on + strand, within LRK53_RS18935 at 182.956 kb on - strand, within LRK53_RS18935 at 183.133 kb on + strand at 183.134 kb on - strand at 183.134 kb on - strand at 183.134 kb on - strand at 183.134 kb on - strand at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.310 kb on + strand, within LRK53_RS18940 at 183.311 kb on - strand, within LRK53_RS18940 at 183.311 kb on - strand, within LRK53_RS18940 at 183.325 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.397 kb on + strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.398 kb on - strand, within LRK53_RS18940 at 183.624 kb on + strand at 183.810 kb on + strand, within LRK53_RS18945 at 183.811 kb on - strand at 183.811 kb on - strand at 183.811 kb on - strand at 183.811 kb on - strand at 183.873 kb on - strand at 183.890 kb on + strand at 184.095 kb on + strand at 184.096 kb on - strand at 184.096 kb on - strand at 184.096 kb on - strand at 184.107 kb on + strand at 184.107 kb on + strand at 184.108 kb on - strand at 184.108 kb on - strand at 184.108 kb on - strand at 184.113 kb on + strand at 184.113 kb on + strand at 184.114 kb on - strand at 184.114 kb on - strand at 184.139 kb on + strand at 184.140 kb on - strand at 184.173 kb on + strand at 184.173 kb on + strand at 184.173 kb on + strand at 184.173 kb on + strand at 184.174 kb on - strand at 184.174 kb on - strand at 184.174 kb on - strand at 184.174 kb on - strand at 184.174 kb on - strand at 184.174 kb on - strand at 184.174 kb on - strand at 184.263 kb on + strand, within LRK53_RS18950 at 184.264 kb on - strand, within LRK53_RS18950 at 184.372 kb on - strand, within LRK53_RS18950 at 184.372 kb on - strand, within LRK53_RS18950
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 182,237 - LRK53_RS18930 0.73 -0.5 182,237 - LRK53_RS18930 0.73 -0.8 182,237 - LRK53_RS18930 0.73 -1.4 182,349 + -1.4 182,349 + -0.4 182,460 + +2.6 182,460 + -1.3 182,460 + +0.5 182,460 + -2.6 182,461 - +0.1 182,461 - -0.6 182,461 - -3.2 182,461 - -1.3 182,461 - -0.3 182,520 + LRK53_RS18935 0.17 -0.5 182,521 - LRK53_RS18935 0.18 -0.9 182,521 - LRK53_RS18935 0.18 -1.1 182,589 + LRK53_RS18935 0.29 -3.1 182,589 + LRK53_RS18935 0.29 -1.1 182,589 + LRK53_RS18935 0.29 +0.1 182,589 + LRK53_RS18935 0.29 -0.7 182,589 + LRK53_RS18935 0.29 -0.3 182,589 + LRK53_RS18935 0.29 -2.3 182,589 + LRK53_RS18935 0.29 -1.3 182,589 + LRK53_RS18935 0.29 -1.5 182,589 + LRK53_RS18935 0.29 -1.9 182,590 - LRK53_RS18935 0.29 -2.3 182,590 - LRK53_RS18935 0.29 -0.9 182,590 - LRK53_RS18935 0.29 -0.1 182,590 - LRK53_RS18935 0.29 -0.7 182,590 - LRK53_RS18935 0.29 -2.5 182,590 - LRK53_RS18935 0.29 +0.5 182,590 - LRK53_RS18935 0.29 -0.2 182,590 - LRK53_RS18935 0.29 -0.5 182,590 - LRK53_RS18935 0.29 -2.3 182,590 - LRK53_RS18935 0.29 -0.1 182,590 - LRK53_RS18935 0.29 +0.5 182,664 + LRK53_RS18935 0.41 -0.3 182,664 + LRK53_RS18935 0.41 -1.3 182,664 + LRK53_RS18935 0.41 +1.3 182,664 + LRK53_RS18935 0.41 -2.4 182,665 - LRK53_RS18935 0.41 +0.1 182,665 - LRK53_RS18935 0.41 -0.9 182,665 - LRK53_RS18935 0.41 -0.6 182,665 - LRK53_RS18935 0.41 -1.7 182,665 - LRK53_RS18935 0.41 -0.3 182,665 - LRK53_RS18935 0.41 +0.1 182,665 - LRK53_RS18935 0.41 +0.5 182,709 + LRK53_RS18935 0.49 -1.3 182,798 + LRK53_RS18935 0.64 -1.6 182,799 - LRK53_RS18935 0.64 +1.1 182,799 - LRK53_RS18935 0.64 -0.5 182,799 - LRK53_RS18935 0.64 -1.5 182,829 + LRK53_RS18935 0.69 -0.9 182,829 + LRK53_RS18935 0.69 -1.2 182,829 + LRK53_RS18935 0.69 -0.9 182,829 + LRK53_RS18935 0.69 -1.6 182,829 + LRK53_RS18935 0.69 -0.2 182,829 + LRK53_RS18935 0.69 -1.9 182,829 + LRK53_RS18935 0.69 +0.7 182,829 + LRK53_RS18935 0.69 -1.9 182,830 - LRK53_RS18935 0.69 -0.2 182,830 - LRK53_RS18935 0.69 -0.2 182,830 - LRK53_RS18935 0.69 -1.6 182,830 - LRK53_RS18935 0.69 +1.1 182,830 - LRK53_RS18935 0.69 -0.9 182,830 - LRK53_RS18935 0.69 -2.3 182,830 - LRK53_RS18935 0.69 -1.5 182,883 + LRK53_RS18935 0.78 -0.9 182,956 - LRK53_RS18935 0.90 -0.9 183,133 + +0.3 183,134 - -1.9 183,134 - +1.5 183,134 - -0.5 183,134 - -0.5 183,310 + LRK53_RS18940 0.37 -2.3 183,310 + LRK53_RS18940 0.37 -0.7 183,310 + LRK53_RS18940 0.37 -0.9 183,310 + LRK53_RS18940 0.37 -0.6 183,310 + LRK53_RS18940 0.37 -0.3 183,311 - LRK53_RS18940 0.37 -1.7 183,311 - LRK53_RS18940 0.37 -2.3 183,325 + LRK53_RS18940 0.40 -1.3 183,397 + LRK53_RS18940 0.56 -1.3 183,397 + LRK53_RS18940 0.56 +0.5 183,397 + LRK53_RS18940 0.56 -0.5 183,397 + LRK53_RS18940 0.56 -1.3 183,397 + LRK53_RS18940 0.56 -1.3 183,397 + LRK53_RS18940 0.56 -0.9 183,397 + LRK53_RS18940 0.56 -2.1 183,398 - LRK53_RS18940 0.56 -1.5 183,398 - LRK53_RS18940 0.56 -0.5 183,398 - LRK53_RS18940 0.56 -0.9 183,398 - LRK53_RS18940 0.56 -1.9 183,398 - LRK53_RS18940 0.56 -0.3 183,398 - LRK53_RS18940 0.56 -1.6 183,398 - LRK53_RS18940 0.56 -1.1 183,398 - LRK53_RS18940 0.56 -0.9 183,398 - LRK53_RS18940 0.56 +0.1 183,398 - LRK53_RS18940 0.56 +0.1 183,398 - LRK53_RS18940 0.56 -0.9 183,624 + +0.5 183,810 + LRK53_RS18945 0.89 -1.1 183,811 - -0.9 183,811 - -1.1 183,811 - -1.7 183,811 - -2.8 183,873 - -1.6 183,890 + -0.7 184,095 + -0.5 184,096 - -1.0 184,096 - -1.3 184,096 - +0.1 184,107 + -2.1 184,107 + -2.4 184,108 - -0.9 184,108 - +0.1 184,108 - +0.1 184,113 + -0.7 184,113 + -1.7 184,114 - -3.1 184,114 - -0.9 184,139 + -1.9 184,140 - -1.4 184,173 + -1.9 184,173 + -2.5 184,173 + +0.5 184,173 + -0.4 184,174 - -0.5 184,174 - -0.5 184,174 - +1.6 184,174 - +0.5 184,174 - -0.7 184,174 - -0.1 184,174 - -2.2 184,263 + LRK53_RS18950 0.29 -1.9 184,264 - LRK53_RS18950 0.29 -1.3 184,372 - LRK53_RS18950 0.53 -1.5 184,372 - LRK53_RS18950 0.53 -1.2
Or see this region's nucleotide sequence