Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18910 and LRK53_RS18915 are separated by 506 nucleotides LRK53_RS18915 and LRK53_RS18920 are separated by 355 nucleotides
LRK53_RS18910: LRK53_RS18910 - ParM/StbA family protein, at 177,780 to 178,841
_RS18910
LRK53_RS18915: LRK53_RS18915 - hypothetical protein, at 179,348 to 179,944
_RS18915
LRK53_RS18920: LRK53_RS18920 - S49 family peptidase, at 180,300 to 181,295
_RS18920
Position (kb) 179
180 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 178.472 kb on - strand, within LRK53_RS18910 at 178.540 kb on + strand, within LRK53_RS18910 at 178.540 kb on + strand, within LRK53_RS18910 at 178.540 kb on + strand, within LRK53_RS18910 at 178.540 kb on + strand, within LRK53_RS18910 at 178.541 kb on - strand, within LRK53_RS18910 at 178.541 kb on - strand, within LRK53_RS18910 at 178.541 kb on - strand, within LRK53_RS18910 at 178.543 kb on + strand, within LRK53_RS18910 at 178.543 kb on + strand, within LRK53_RS18910 at 178.543 kb on + strand, within LRK53_RS18910 at 178.544 kb on - strand, within LRK53_RS18910 at 178.544 kb on - strand, within LRK53_RS18910 at 178.544 kb on - strand, within LRK53_RS18910 at 178.544 kb on - strand, within LRK53_RS18910 at 178.555 kb on + strand, within LRK53_RS18910 at 178.556 kb on - strand, within LRK53_RS18910 at 178.556 kb on - strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.573 kb on + strand, within LRK53_RS18910 at 178.574 kb on - strand, within LRK53_RS18910 at 178.574 kb on - strand, within LRK53_RS18910 at 178.574 kb on - strand, within LRK53_RS18910 at 178.574 kb on - strand, within LRK53_RS18910 at 178.697 kb on + strand, within LRK53_RS18910 at 178.872 kb on + strand at 179.027 kb on + strand at 179.027 kb on + strand at 179.027 kb on + strand at 179.171 kb on + strand at 179.172 kb on - strand at 179.209 kb on + strand at 179.210 kb on - strand at 179.210 kb on - strand at 179.210 kb on - strand at 179.210 kb on - strand at 179.341 kb on + strand at 179.341 kb on + strand at 179.341 kb on + strand at 179.341 kb on + strand at 179.341 kb on + strand at 179.341 kb on + strand at 179.341 kb on + strand at 179.341 kb on + strand at 179.342 kb on - strand at 179.342 kb on - strand at 179.342 kb on - strand at 179.342 kb on - strand at 179.342 kb on - strand at 179.342 kb on - strand at 179.342 kb on - strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.345 kb on + strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.346 kb on - strand at 179.379 kb on + strand at 179.379 kb on + strand at 179.379 kb on + strand at 179.379 kb on + strand at 179.379 kb on + strand at 179.379 kb on + strand at 179.380 kb on - strand at 179.380 kb on - strand at 179.380 kb on - strand at 179.380 kb on - strand at 179.380 kb on - strand at 179.380 kb on - strand at 179.576 kb on + strand, within LRK53_RS18915 at 179.576 kb on + strand, within LRK53_RS18915 at 179.576 kb on + strand, within LRK53_RS18915 at 179.576 kb on + strand, within LRK53_RS18915 at 179.576 kb on + strand, within LRK53_RS18915 at 179.577 kb on - strand, within LRK53_RS18915 at 179.628 kb on - strand, within LRK53_RS18915 at 179.628 kb on - strand, within LRK53_RS18915 at 179.628 kb on - strand, within LRK53_RS18915 at 179.628 kb on - strand, within LRK53_RS18915 at 179.628 kb on - strand, within LRK53_RS18915 at 179.669 kb on + strand, within LRK53_RS18915 at 179.669 kb on + strand, within LRK53_RS18915 at 179.675 kb on + strand, within LRK53_RS18915 at 179.676 kb on - strand, within LRK53_RS18915 at 179.676 kb on - strand, within LRK53_RS18915 at 179.676 kb on - strand, within LRK53_RS18915 at 179.676 kb on - strand, within LRK53_RS18915 at 179.676 kb on - strand, within LRK53_RS18915 at 179.750 kb on + strand, within LRK53_RS18915 at 179.751 kb on - strand, within LRK53_RS18915 at 179.751 kb on - strand, within LRK53_RS18915 at 179.751 kb on - strand, within LRK53_RS18915 at 179.858 kb on + strand, within LRK53_RS18915 at 179.858 kb on + strand, within LRK53_RS18915 at 179.859 kb on - strand, within LRK53_RS18915 at 179.859 kb on - strand, within LRK53_RS18915 at 179.859 kb on - strand, within LRK53_RS18915 at 179.943 kb on - strand at 180.060 kb on - strand at 180.060 kb on - strand at 180.060 kb on - strand at 180.298 kb on + strand at 180.298 kb on + strand at 180.298 kb on + strand at 180.298 kb on + strand at 180.298 kb on + strand at 180.299 kb on - strand at 180.299 kb on - strand at 180.299 kb on - strand at 180.299 kb on - strand at 180.299 kb on - strand at 180.299 kb on - strand at 180.309 kb on - strand at 180.309 kb on - strand at 180.489 kb on + strand, within LRK53_RS18920 at 180.490 kb on - strand, within LRK53_RS18920 at 180.728 kb on + strand, within LRK53_RS18920 at 180.729 kb on - strand, within LRK53_RS18920 at 180.729 kb on - strand, within LRK53_RS18920 at 180.729 kb on - strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.762 kb on + strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.763 kb on - strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.798 kb on + strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.799 kb on - strand, within LRK53_RS18920 at 180.829 kb on - strand, within LRK53_RS18920 at 180.829 kb on - strand, within LRK53_RS18920 at 180.840 kb on + strand, within LRK53_RS18920 at 180.840 kb on + strand, within LRK53_RS18920 at 180.840 kb on + strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.841 kb on - strand, within LRK53_RS18920 at 180.876 kb on + strand, within LRK53_RS18920 at 180.877 kb on - strand, within LRK53_RS18920 at 180.877 kb on - strand, within LRK53_RS18920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 178,472 - LRK53_RS18910 0.65 -0.3 178,540 + LRK53_RS18910 0.72 +1.1 178,540 + LRK53_RS18910 0.72 +0.1 178,540 + LRK53_RS18910 0.72 -0.7 178,540 + LRK53_RS18910 0.72 -0.9 178,541 - LRK53_RS18910 0.72 +0.1 178,541 - LRK53_RS18910 0.72 -0.5 178,541 - LRK53_RS18910 0.72 -2.1 178,543 + LRK53_RS18910 0.72 +1.1 178,543 + LRK53_RS18910 0.72 +1.1 178,543 + LRK53_RS18910 0.72 +0.1 178,544 - LRK53_RS18910 0.72 -0.1 178,544 - LRK53_RS18910 0.72 -0.5 178,544 - LRK53_RS18910 0.72 -0.9 178,544 - LRK53_RS18910 0.72 +0.2 178,555 + LRK53_RS18910 0.73 -1.3 178,556 - LRK53_RS18910 0.73 +0.5 178,556 - LRK53_RS18910 0.73 -0.7 178,573 + LRK53_RS18910 0.75 -3.5 178,573 + LRK53_RS18910 0.75 -0.5 178,573 + LRK53_RS18910 0.75 +0.6 178,573 + LRK53_RS18910 0.75 -1.6 178,573 + LRK53_RS18910 0.75 -0.1 178,573 + LRK53_RS18910 0.75 +0.7 178,573 + LRK53_RS18910 0.75 +0.5 178,573 + LRK53_RS18910 0.75 +0.5 178,573 + LRK53_RS18910 0.75 -2.4 178,574 - LRK53_RS18910 0.75 +0.5 178,574 - LRK53_RS18910 0.75 +1.4 178,574 - LRK53_RS18910 0.75 +1.1 178,574 - LRK53_RS18910 0.75 +0.6 178,697 + LRK53_RS18910 0.86 -0.5 178,872 + -1.3 179,027 + +0.2 179,027 + -1.7 179,027 + -1.1 179,171 + +0.7 179,172 - -1.7 179,209 + -2.1 179,210 - -0.5 179,210 - -1.5 179,210 - -0.5 179,210 - -1.3 179,341 + -0.5 179,341 + +0.1 179,341 + -0.1 179,341 + +0.5 179,341 + +0.6 179,341 + -0.7 179,341 + +0.1 179,341 + -0.3 179,342 - -1.4 179,342 - -1.7 179,342 - -1.4 179,342 - -2.8 179,342 - -0.9 179,342 - -0.1 179,342 - -2.5 179,345 + +0.1 179,345 + -1.1 179,345 + -0.7 179,345 + -2.6 179,345 + +1.1 179,345 + -0.4 179,345 + -0.8 179,345 + +0.6 179,345 + +0.6 179,345 + +0.6 179,345 + +0.1 179,345 + +1.1 179,345 + +0.1 179,345 + +0.7 179,345 + +0.1 179,346 - -1.1 179,346 - -1.0 179,346 - +0.1 179,346 - -0.5 179,346 - -0.4 179,346 - -0.7 179,346 - -0.8 179,346 - -0.9 179,346 - +2.1 179,346 - -0.9 179,346 - -0.7 179,346 - -0.9 179,346 - -1.6 179,346 - -1.9 179,379 + -0.5 179,379 + +1.3 179,379 + +1.1 179,379 + -1.9 179,379 + +0.5 179,379 + -2.7 179,380 - -1.5 179,380 - -0.5 179,380 - +1.1 179,380 - +0.1 179,380 - -1.1 179,380 - -1.3 179,576 + LRK53_RS18915 0.38 -0.5 179,576 + LRK53_RS18915 0.38 -1.3 179,576 + LRK53_RS18915 0.38 +0.7 179,576 + LRK53_RS18915 0.38 +0.2 179,576 + LRK53_RS18915 0.38 -0.5 179,577 - LRK53_RS18915 0.38 -0.5 179,628 - LRK53_RS18915 0.47 -1.4 179,628 - LRK53_RS18915 0.47 -1.5 179,628 - LRK53_RS18915 0.47 -0.8 179,628 - LRK53_RS18915 0.47 -0.5 179,628 - LRK53_RS18915 0.47 +0.4 179,669 + LRK53_RS18915 0.54 -0.4 179,669 + LRK53_RS18915 0.54 -0.5 179,675 + LRK53_RS18915 0.55 -0.2 179,676 - LRK53_RS18915 0.55 -1.0 179,676 - LRK53_RS18915 0.55 -0.5 179,676 - LRK53_RS18915 0.55 +0.1 179,676 - LRK53_RS18915 0.55 -2.4 179,676 - LRK53_RS18915 0.55 +0.1 179,750 + LRK53_RS18915 0.67 -1.4 179,751 - LRK53_RS18915 0.68 -0.3 179,751 - LRK53_RS18915 0.68 -0.7 179,751 - LRK53_RS18915 0.68 +1.1 179,858 + LRK53_RS18915 0.85 -0.3 179,858 + LRK53_RS18915 0.85 -0.9 179,859 - LRK53_RS18915 0.86 +1.6 179,859 - LRK53_RS18915 0.86 +0.3 179,859 - LRK53_RS18915 0.86 -0.9 179,943 - +0.5 180,060 - -0.5 180,060 - +1.1 180,060 - -1.6 180,298 + -0.8 180,298 + -0.7 180,298 + +0.6 180,298 + -1.5 180,298 + -0.9 180,299 - -1.5 180,299 - +0.2 180,299 - -1.5 180,299 - -0.8 180,299 - -1.6 180,299 - -0.9 180,309 - +1.5 180,309 - -0.3 180,489 + LRK53_RS18920 0.19 +0.1 180,490 - LRK53_RS18920 0.19 +0.3 180,728 + LRK53_RS18920 0.43 -0.3 180,729 - LRK53_RS18920 0.43 -0.3 180,729 - LRK53_RS18920 0.43 +0.2 180,729 - LRK53_RS18920 0.43 -3.1 180,762 + LRK53_RS18920 0.46 -1.7 180,762 + LRK53_RS18920 0.46 +0.1 180,762 + LRK53_RS18920 0.46 -1.4 180,762 + LRK53_RS18920 0.46 +1.1 180,762 + LRK53_RS18920 0.46 +0.8 180,762 + LRK53_RS18920 0.46 -1.1 180,762 + LRK53_RS18920 0.46 +0.1 180,762 + LRK53_RS18920 0.46 +0.1 180,763 - LRK53_RS18920 0.46 +1.1 180,763 - LRK53_RS18920 0.46 -0.9 180,763 - LRK53_RS18920 0.46 -1.5 180,763 - LRK53_RS18920 0.46 -1.3 180,763 - LRK53_RS18920 0.46 -1.6 180,763 - LRK53_RS18920 0.46 +0.6 180,763 - LRK53_RS18920 0.46 -1.7 180,763 - LRK53_RS18920 0.46 -0.5 180,763 - LRK53_RS18920 0.46 +2.1 180,763 - LRK53_RS18920 0.46 -0.5 180,798 + LRK53_RS18920 0.50 -1.7 180,798 + LRK53_RS18920 0.50 -2.8 180,798 + LRK53_RS18920 0.50 +1.6 180,798 + LRK53_RS18920 0.50 -0.0 180,799 - LRK53_RS18920 0.50 -2.4 180,799 - LRK53_RS18920 0.50 -0.5 180,799 - LRK53_RS18920 0.50 -0.3 180,799 - LRK53_RS18920 0.50 -0.3 180,799 - LRK53_RS18920 0.50 +0.1 180,799 - LRK53_RS18920 0.50 -2.6 180,829 - LRK53_RS18920 0.53 -0.5 180,829 - LRK53_RS18920 0.53 -0.9 180,840 + LRK53_RS18920 0.54 -1.1 180,840 + LRK53_RS18920 0.54 -2.3 180,840 + LRK53_RS18920 0.54 -1.5 180,841 - LRK53_RS18920 0.54 +0.1 180,841 - LRK53_RS18920 0.54 -1.1 180,841 - LRK53_RS18920 0.54 -1.2 180,841 - LRK53_RS18920 0.54 +2.1 180,841 - LRK53_RS18920 0.54 +0.1 180,841 - LRK53_RS18920 0.54 +0.6 180,876 + LRK53_RS18920 0.58 -1.5 180,877 - LRK53_RS18920 0.58 -2.5 180,877 - LRK53_RS18920 0.58 -0.5
Or see this region's nucleotide sequence