Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18735 and LRK53_RS18740 are separated by 85 nucleotides LRK53_RS18740 and LRK53_RS18745 are separated by 393 nucleotides LRK53_RS18745 and LRK53_RS18750 overlap by 20 nucleotides
LRK53_RS18735: LRK53_RS18735 - hypothetical protein, at 138,134 to 139,225
_RS18735
LRK53_RS18740: LRK53_RS18740 - hypothetical protein, at 139,311 to 140,813
_RS18740
LRK53_RS18745: LRK53_RS18745 - hypothetical protein, at 141,207 to 141,458
_RS18745
LRK53_RS18750: LRK53_RS18750 - hypothetical protein, at 141,439 to 141,864
_RS18750
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 138.458 kb on + strand, within LRK53_RS18735 at 138.459 kb on - strand, within LRK53_RS18735 at 138.459 kb on - strand, within LRK53_RS18735 at 138.459 kb on - strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.513 kb on + strand, within LRK53_RS18735 at 138.514 kb on - strand, within LRK53_RS18735 at 138.514 kb on - strand, within LRK53_RS18735 at 138.514 kb on - strand, within LRK53_RS18735 at 138.514 kb on - strand, within LRK53_RS18735 at 138.642 kb on + strand, within LRK53_RS18735 at 138.642 kb on + strand, within LRK53_RS18735 at 138.643 kb on - strand, within LRK53_RS18735 at 138.659 kb on + strand, within LRK53_RS18735 at 138.659 kb on + strand, within LRK53_RS18735 at 138.660 kb on - strand, within LRK53_RS18735 at 139.023 kb on - strand, within LRK53_RS18735 at 139.023 kb on - strand, within LRK53_RS18735 at 139.023 kb on - strand, within LRK53_RS18735 at 139.031 kb on + strand, within LRK53_RS18735 at 139.031 kb on + strand, within LRK53_RS18735 at 139.031 kb on + strand, within LRK53_RS18735 at 139.031 kb on + strand, within LRK53_RS18735 at 139.031 kb on + strand, within LRK53_RS18735 at 139.032 kb on - strand, within LRK53_RS18735 at 139.088 kb on + strand, within LRK53_RS18735 at 139.088 kb on + strand, within LRK53_RS18735 at 139.088 kb on + strand, within LRK53_RS18735 at 139.088 kb on + strand, within LRK53_RS18735 at 139.089 kb on - strand, within LRK53_RS18735 at 139.089 kb on - strand, within LRK53_RS18735 at 139.089 kb on - strand, within LRK53_RS18735 at 139.089 kb on - strand, within LRK53_RS18735 at 139.089 kb on - strand, within LRK53_RS18735 at 139.089 kb on - strand, within LRK53_RS18735 at 139.182 kb on - strand at 139.215 kb on - strand at 139.325 kb on + strand at 139.325 kb on + strand at 139.326 kb on - strand at 139.326 kb on - strand at 139.326 kb on - strand at 139.326 kb on - strand at 139.326 kb on - strand at 139.326 kb on - strand at 139.334 kb on + strand at 139.335 kb on - strand at 139.335 kb on - strand at 139.335 kb on - strand at 139.360 kb on + strand at 139.361 kb on - strand at 139.508 kb on + strand, within LRK53_RS18740 at 139.508 kb on + strand, within LRK53_RS18740 at 139.508 kb on + strand, within LRK53_RS18740 at 139.509 kb on - strand, within LRK53_RS18740 at 139.509 kb on - strand, within LRK53_RS18740 at 139.556 kb on + strand, within LRK53_RS18740 at 139.556 kb on + strand, within LRK53_RS18740 at 139.556 kb on + strand, within LRK53_RS18740 at 139.556 kb on + strand, within LRK53_RS18740 at 139.556 kb on + strand, within LRK53_RS18740 at 139.556 kb on + strand, within LRK53_RS18740 at 139.557 kb on - strand, within LRK53_RS18740 at 139.557 kb on - strand, within LRK53_RS18740 at 139.557 kb on - strand, within LRK53_RS18740 at 139.557 kb on - strand, within LRK53_RS18740 at 139.557 kb on - strand, within LRK53_RS18740 at 139.557 kb on - strand, within LRK53_RS18740 at 139.669 kb on + strand, within LRK53_RS18740 at 139.669 kb on + strand, within LRK53_RS18740 at 139.669 kb on + strand, within LRK53_RS18740 at 139.669 kb on + strand, within LRK53_RS18740 at 139.669 kb on + strand, within LRK53_RS18740 at 139.670 kb on - strand, within LRK53_RS18740 at 139.670 kb on - strand, within LRK53_RS18740 at 139.670 kb on - strand, within LRK53_RS18740 at 139.726 kb on + strand, within LRK53_RS18740 at 139.726 kb on + strand, within LRK53_RS18740 at 139.726 kb on + strand, within LRK53_RS18740 at 139.726 kb on + strand, within LRK53_RS18740 at 139.727 kb on - strand, within LRK53_RS18740 at 139.727 kb on - strand, within LRK53_RS18740 at 139.727 kb on - strand, within LRK53_RS18740 at 139.727 kb on - strand, within LRK53_RS18740 at 139.727 kb on - strand, within LRK53_RS18740 at 139.765 kb on + strand, within LRK53_RS18740 at 139.765 kb on + strand, within LRK53_RS18740 at 139.765 kb on + strand, within LRK53_RS18740 at 139.766 kb on - strand, within LRK53_RS18740 at 139.766 kb on - strand, within LRK53_RS18740 at 139.766 kb on - strand, within LRK53_RS18740 at 139.766 kb on - strand, within LRK53_RS18740 at 139.766 kb on - strand, within LRK53_RS18740 at 139.915 kb on + strand, within LRK53_RS18740 at 139.916 kb on - strand, within LRK53_RS18740 at 140.059 kb on + strand, within LRK53_RS18740 at 140.313 kb on + strand, within LRK53_RS18740 at 140.314 kb on - strand, within LRK53_RS18740 at 140.359 kb on + strand, within LRK53_RS18740 at 140.360 kb on - strand, within LRK53_RS18740 at 140.360 kb on - strand, within LRK53_RS18740 at 140.360 kb on - strand, within LRK53_RS18740 at 140.440 kb on + strand, within LRK53_RS18740 at 140.440 kb on + strand, within LRK53_RS18740 at 140.441 kb on - strand, within LRK53_RS18740 at 140.462 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.481 kb on + strand, within LRK53_RS18740 at 140.482 kb on - strand, within LRK53_RS18740 at 140.482 kb on - strand, within LRK53_RS18740 at 140.482 kb on - strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.485 kb on + strand, within LRK53_RS18740 at 140.486 kb on - strand, within LRK53_RS18740 at 140.735 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.796 kb on + strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.797 kb on - strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.807 kb on + strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.808 kb on - strand at 140.820 kb on + strand at 140.820 kb on + strand at 140.821 kb on - strand at 140.821 kb on - strand at 140.821 kb on - strand at 140.884 kb on + strand at 140.884 kb on + strand at 140.884 kb on + strand at 140.884 kb on + strand at 140.885 kb on - strand at 140.885 kb on - strand at 140.957 kb on + strand at 140.957 kb on + strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.958 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 140.960 kb on - strand at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.289 kb on + strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.290 kb on - strand, within LRK53_RS18745 at 141.427 kb on + strand, within LRK53_RS18745 at 141.427 kb on + strand, within LRK53_RS18745 at 141.427 kb on + strand, within LRK53_RS18745 at 141.428 kb on - strand, within LRK53_RS18745 at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.445 kb on + strand at 141.446 kb on - strand at 141.446 kb on - strand at 141.446 kb on - strand at 141.446 kb on - strand at 141.679 kb on + strand, within LRK53_RS18750 at 141.679 kb on + strand, within LRK53_RS18750 at 141.679 kb on + strand, within LRK53_RS18750 at 141.679 kb on + strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.680 kb on - strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.733 kb on + strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.734 kb on - strand, within LRK53_RS18750 at 141.754 kb on + strand, within LRK53_RS18750 at 141.754 kb on + strand, within LRK53_RS18750 at 141.755 kb on - strand, within LRK53_RS18750 at 141.755 kb on - strand, within LRK53_RS18750 at 141.755 kb on - strand, within LRK53_RS18750 at 141.772 kb on + strand, within LRK53_RS18750 at 141.772 kb on + strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750 at 141.773 kb on - strand, within LRK53_RS18750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 138,458 + LRK53_RS18735 0.30 +0.1 138,459 - LRK53_RS18735 0.30 -1.9 138,459 - LRK53_RS18735 0.30 +1.6 138,459 - LRK53_RS18735 0.30 -1.7 138,513 + LRK53_RS18735 0.35 -0.9 138,513 + LRK53_RS18735 0.35 -1.3 138,513 + LRK53_RS18735 0.35 -0.4 138,513 + LRK53_RS18735 0.35 -1.2 138,513 + LRK53_RS18735 0.35 -2.6 138,513 + LRK53_RS18735 0.35 -0.7 138,513 + LRK53_RS18735 0.35 -0.3 138,513 + LRK53_RS18735 0.35 +0.1 138,514 - LRK53_RS18735 0.35 +1.2 138,514 - LRK53_RS18735 0.35 -1.3 138,514 - LRK53_RS18735 0.35 -0.5 138,514 - LRK53_RS18735 0.35 +0.1 138,642 + LRK53_RS18735 0.47 -0.5 138,642 + LRK53_RS18735 0.47 +1.1 138,643 - LRK53_RS18735 0.47 +0.6 138,659 + LRK53_RS18735 0.48 -1.3 138,659 + LRK53_RS18735 0.48 -1.4 138,660 - LRK53_RS18735 0.48 -0.9 139,023 - LRK53_RS18735 0.81 -1.3 139,023 - LRK53_RS18735 0.81 -0.9 139,023 - LRK53_RS18735 0.81 -0.5 139,031 + LRK53_RS18735 0.82 -0.2 139,031 + LRK53_RS18735 0.82 +0.1 139,031 + LRK53_RS18735 0.82 -2.1 139,031 + LRK53_RS18735 0.82 -1.7 139,031 + LRK53_RS18735 0.82 +1.1 139,032 - LRK53_RS18735 0.82 -0.5 139,088 + LRK53_RS18735 0.87 -1.3 139,088 + LRK53_RS18735 0.87 +0.5 139,088 + LRK53_RS18735 0.87 -0.8 139,088 + LRK53_RS18735 0.87 -2.6 139,089 - LRK53_RS18735 0.87 -3.0 139,089 - LRK53_RS18735 0.87 +0.4 139,089 - LRK53_RS18735 0.87 -1.6 139,089 - LRK53_RS18735 0.87 -1.5 139,089 - LRK53_RS18735 0.87 +0.2 139,089 - LRK53_RS18735 0.87 +0.2 139,182 - +1.1 139,215 - -1.0 139,325 + +0.1 139,325 + -0.9 139,326 - -2.3 139,326 - +0.1 139,326 - -0.1 139,326 - -1.6 139,326 - -1.7 139,326 - +1.5 139,334 + -0.4 139,335 - -0.5 139,335 - -2.1 139,335 - -0.9 139,360 + -0.2 139,361 - -4.6 139,508 + LRK53_RS18740 0.13 +0.2 139,508 + LRK53_RS18740 0.13 -0.9 139,508 + LRK53_RS18740 0.13 -1.9 139,509 - LRK53_RS18740 0.13 -0.5 139,509 - LRK53_RS18740 0.13 -0.2 139,556 + LRK53_RS18740 0.16 +0.1 139,556 + LRK53_RS18740 0.16 -2.4 139,556 + LRK53_RS18740 0.16 -0.5 139,556 + LRK53_RS18740 0.16 +1.1 139,556 + LRK53_RS18740 0.16 -2.6 139,556 + LRK53_RS18740 0.16 +0.1 139,557 - LRK53_RS18740 0.16 -0.5 139,557 - LRK53_RS18740 0.16 -0.7 139,557 - LRK53_RS18740 0.16 -0.9 139,557 - LRK53_RS18740 0.16 -1.1 139,557 - LRK53_RS18740 0.16 -2.4 139,557 - LRK53_RS18740 0.16 -2.1 139,669 + LRK53_RS18740 0.24 +1.1 139,669 + LRK53_RS18740 0.24 -0.9 139,669 + LRK53_RS18740 0.24 -0.5 139,669 + LRK53_RS18740 0.24 -1.6 139,669 + LRK53_RS18740 0.24 -0.7 139,670 - LRK53_RS18740 0.24 -1.3 139,670 - LRK53_RS18740 0.24 -2.7 139,670 - LRK53_RS18740 0.24 -0.7 139,726 + LRK53_RS18740 0.28 -0.3 139,726 + LRK53_RS18740 0.28 +0.1 139,726 + LRK53_RS18740 0.28 -2.1 139,726 + LRK53_RS18740 0.28 -0.8 139,727 - LRK53_RS18740 0.28 -1.7 139,727 - LRK53_RS18740 0.28 +1.1 139,727 - LRK53_RS18740 0.28 -1.3 139,727 - LRK53_RS18740 0.28 -0.5 139,727 - LRK53_RS18740 0.28 -1.6 139,765 + LRK53_RS18740 0.30 -1.3 139,765 + LRK53_RS18740 0.30 +0.3 139,765 + LRK53_RS18740 0.30 -0.1 139,766 - LRK53_RS18740 0.30 -1.7 139,766 - LRK53_RS18740 0.30 -1.0 139,766 - LRK53_RS18740 0.30 -1.8 139,766 - LRK53_RS18740 0.30 -0.6 139,766 - LRK53_RS18740 0.30 -1.5 139,915 + LRK53_RS18740 0.40 -0.5 139,916 - LRK53_RS18740 0.40 -1.5 140,059 + LRK53_RS18740 0.50 -1.3 140,313 + LRK53_RS18740 0.67 -0.9 140,314 - LRK53_RS18740 0.67 -1.1 140,359 + LRK53_RS18740 0.70 +0.1 140,360 - LRK53_RS18740 0.70 -0.8 140,360 - LRK53_RS18740 0.70 -1.1 140,360 - LRK53_RS18740 0.70 -1.4 140,440 + LRK53_RS18740 0.75 +0.1 140,440 + LRK53_RS18740 0.75 +0.1 140,441 - LRK53_RS18740 0.75 -1.1 140,462 + LRK53_RS18740 0.77 -1.5 140,481 + LRK53_RS18740 0.78 -2.1 140,481 + LRK53_RS18740 0.78 -1.5 140,481 + LRK53_RS18740 0.78 -0.8 140,481 + LRK53_RS18740 0.78 -1.6 140,482 - LRK53_RS18740 0.78 -1.3 140,482 - LRK53_RS18740 0.78 -0.9 140,482 - LRK53_RS18740 0.78 -1.1 140,485 + LRK53_RS18740 0.78 -1.3 140,485 + LRK53_RS18740 0.78 -0.4 140,485 + LRK53_RS18740 0.78 -2.4 140,485 + LRK53_RS18740 0.78 +1.1 140,485 + LRK53_RS18740 0.78 -1.4 140,485 + LRK53_RS18740 0.78 -1.7 140,486 - LRK53_RS18740 0.78 -1.1 140,735 + -0.2 140,796 + +0.6 140,796 + -0.9 140,796 + -1.3 140,796 + -1.5 140,796 + -0.9 140,796 + -2.5 140,797 - -0.5 140,797 - -1.1 140,797 - +0.1 140,797 - +0.6 140,797 - -1.7 140,807 + +1.1 140,807 + +0.1 140,807 + -0.0 140,807 + -0.3 140,807 + -0.9 140,807 + -1.1 140,807 + -1.1 140,807 + -0.9 140,808 - -1.3 140,808 - -1.9 140,808 - +0.6 140,808 - -0.8 140,808 - -0.5 140,808 - +0.7 140,820 + -0.9 140,820 + +0.2 140,821 - -1.1 140,821 - -2.8 140,821 - -1.3 140,884 + -0.0 140,884 + -1.9 140,884 + -0.7 140,884 + -2.4 140,885 - -0.1 140,885 - -1.9 140,957 + -0.7 140,957 + -0.9 140,958 - -0.2 140,958 - +1.6 140,958 - +1.1 140,958 - -1.3 140,958 - +0.5 140,958 - -0.6 140,958 - -1.2 140,958 - -1.1 140,960 - +0.1 140,960 - -1.5 140,960 - -0.2 140,960 - +0.1 140,960 - -0.7 140,960 - -1.4 141,289 + LRK53_RS18745 0.33 -1.4 141,289 + LRK53_RS18745 0.33 -0.0 141,289 + LRK53_RS18745 0.33 +1.1 141,289 + LRK53_RS18745 0.33 -1.7 141,289 + LRK53_RS18745 0.33 +0.1 141,290 - LRK53_RS18745 0.33 -0.9 141,290 - LRK53_RS18745 0.33 -0.7 141,290 - LRK53_RS18745 0.33 +0.1 141,290 - LRK53_RS18745 0.33 -1.2 141,290 - LRK53_RS18745 0.33 -1.3 141,427 + LRK53_RS18745 0.87 -0.7 141,427 + LRK53_RS18745 0.87 -0.8 141,427 + LRK53_RS18745 0.87 -0.2 141,428 - LRK53_RS18745 0.88 -1.7 141,445 + -0.7 141,445 + -0.7 141,445 + +0.3 141,445 + -1.1 141,445 + -0.9 141,445 + -0.4 141,445 + -0.9 141,445 + -1.9 141,445 + -1.8 141,446 - -0.4 141,446 - -3.2 141,446 - -0.2 141,446 - -0.5 141,679 + LRK53_RS18750 0.56 -0.3 141,679 + LRK53_RS18750 0.56 +0.5 141,679 + LRK53_RS18750 0.56 -0.9 141,679 + LRK53_RS18750 0.56 -0.7 141,680 - LRK53_RS18750 0.57 -2.8 141,680 - LRK53_RS18750 0.57 +0.1 141,680 - LRK53_RS18750 0.57 +0.1 141,680 - LRK53_RS18750 0.57 -0.6 141,680 - LRK53_RS18750 0.57 -0.6 141,680 - LRK53_RS18750 0.57 -0.6 141,733 + LRK53_RS18750 0.69 -1.7 141,733 + LRK53_RS18750 0.69 -1.3 141,733 + LRK53_RS18750 0.69 -0.9 141,733 + LRK53_RS18750 0.69 -1.5 141,733 + LRK53_RS18750 0.69 -0.4 141,733 + LRK53_RS18750 0.69 +0.1 141,733 + LRK53_RS18750 0.69 -0.7 141,734 - LRK53_RS18750 0.69 +0.3 141,734 - LRK53_RS18750 0.69 -1.0 141,734 - LRK53_RS18750 0.69 -1.3 141,734 - LRK53_RS18750 0.69 -0.5 141,754 + LRK53_RS18750 0.74 +1.6 141,754 + LRK53_RS18750 0.74 -1.3 141,755 - LRK53_RS18750 0.74 -0.4 141,755 - LRK53_RS18750 0.74 -1.6 141,755 - LRK53_RS18750 0.74 +0.2 141,772 + LRK53_RS18750 0.78 +1.1 141,772 + LRK53_RS18750 0.78 -1.9 141,773 - LRK53_RS18750 0.78 -1.1 141,773 - LRK53_RS18750 0.78 -2.1 141,773 - LRK53_RS18750 0.78 -0.9 141,773 - LRK53_RS18750 0.78 +0.1
Or see this region's nucleotide sequence