Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18270 and LRK53_RS18275 are separated by 888 nucleotides LRK53_RS18275 and LRK53_RS18280 are separated by 32 nucleotides
LRK53_RS18270: LRK53_RS18270 - ATP-binding domain-containing protein, at 31,564 to 31,725
_RS18270
LRK53_RS18275: LRK53_RS18275 - UvrD-helicase domain-containing protein, at 32,614 to 33,210
_RS18275
LRK53_RS18280: LRK53_RS18280 - hypothetical protein, at 33,243 to 33,995
_RS18280
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 31.901 kb on + strand at 31.901 kb on + strand at 31.901 kb on + strand at 31.902 kb on - strand at 31.902 kb on - strand at 31.902 kb on - strand at 31.902 kb on - strand at 32.060 kb on + strand at 32.168 kb on + strand at 32.168 kb on + strand at 32.168 kb on + strand at 32.168 kb on + strand at 32.168 kb on + strand at 32.168 kb on + strand at 32.169 kb on - strand at 32.169 kb on - strand at 32.169 kb on - strand at 32.169 kb on - strand at 32.169 kb on - strand at 32.169 kb on - strand at 32.477 kb on + strand at 32.477 kb on + strand at 32.477 kb on + strand at 32.477 kb on + strand at 32.541 kb on - strand at 32.564 kb on + strand at 32.564 kb on + strand at 32.565 kb on - strand at 32.565 kb on - strand at 32.678 kb on + strand, within LRK53_RS18275 at 32.678 kb on + strand, within LRK53_RS18275 at 32.678 kb on + strand, within LRK53_RS18275 at 32.678 kb on + strand, within LRK53_RS18275 at 32.678 kb on + strand, within LRK53_RS18275 at 32.678 kb on + strand, within LRK53_RS18275 at 32.678 kb on + strand, within LRK53_RS18275 at 32.679 kb on - strand, within LRK53_RS18275 at 32.679 kb on - strand, within LRK53_RS18275 at 32.679 kb on - strand, within LRK53_RS18275 at 32.679 kb on - strand, within LRK53_RS18275 at 32.691 kb on - strand, within LRK53_RS18275 at 32.793 kb on - strand, within LRK53_RS18275 at 32.793 kb on - strand, within LRK53_RS18275 at 32.905 kb on + strand, within LRK53_RS18275 at 32.905 kb on + strand, within LRK53_RS18275 at 32.905 kb on + strand, within LRK53_RS18275 at 32.906 kb on - strand, within LRK53_RS18275 at 32.906 kb on - strand, within LRK53_RS18275 at 32.906 kb on - strand, within LRK53_RS18275 at 33.416 kb on - strand, within LRK53_RS18280 at 33.416 kb on - strand, within LRK53_RS18280 at 33.416 kb on - strand, within LRK53_RS18280 at 33.416 kb on - strand, within LRK53_RS18280 at 33.416 kb on - strand, within LRK53_RS18280 at 33.416 kb on - strand, within LRK53_RS18280 at 33.416 kb on - strand, within LRK53_RS18280 at 33.535 kb on + strand, within LRK53_RS18280 at 33.536 kb on - strand, within LRK53_RS18280 at 33.536 kb on - strand, within LRK53_RS18280 at 33.575 kb on + strand, within LRK53_RS18280 at 33.576 kb on - strand, within LRK53_RS18280 at 33.576 kb on - strand, within LRK53_RS18280 at 33.576 kb on - strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.577 kb on + strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.578 kb on - strand, within LRK53_RS18280 at 33.760 kb on + strand, within LRK53_RS18280 at 33.769 kb on + strand, within LRK53_RS18280 at 33.769 kb on + strand, within LRK53_RS18280 at 33.805 kb on + strand, within LRK53_RS18280 at 33.806 kb on - strand, within LRK53_RS18280 at 33.806 kb on - strand, within LRK53_RS18280 at 33.806 kb on - strand, within LRK53_RS18280 at 33.811 kb on + strand, within LRK53_RS18280 at 33.811 kb on + strand, within LRK53_RS18280 at 33.811 kb on + strand, within LRK53_RS18280 at 33.812 kb on - strand, within LRK53_RS18280 at 33.812 kb on - strand, within LRK53_RS18280 at 33.812 kb on - strand, within LRK53_RS18280 at 33.812 kb on - strand, within LRK53_RS18280 at 33.845 kb on - strand, within LRK53_RS18280 at 33.845 kb on - strand, within LRK53_RS18280 at 33.845 kb on - strand, within LRK53_RS18280 at 33.845 kb on - strand, within LRK53_RS18280 at 33.845 kb on - strand, within LRK53_RS18280 at 34.017 kb on - strand at 34.068 kb on + strand at 34.149 kb on - strand at 34.194 kb on + strand at 34.194 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 31,901 + -2.6 31,901 + -0.2 31,901 + +0.5 31,902 - -2.3 31,902 - -0.5 31,902 - -0.2 31,902 - -0.5 32,060 + -0.9 32,168 + -1.3 32,168 + +0.5 32,168 + +0.1 32,168 + -1.7 32,168 + -0.5 32,168 + -1.5 32,169 - +1.1 32,169 - -0.7 32,169 - +1.1 32,169 - -0.7 32,169 - -0.3 32,169 - -0.8 32,477 + -0.2 32,477 + -1.8 32,477 + -1.1 32,477 + -2.3 32,541 - -1.8 32,564 + -2.3 32,564 + +1.1 32,565 - -0.1 32,565 - -1.2 32,678 + LRK53_RS18275 0.11 -0.5 32,678 + LRK53_RS18275 0.11 +1.1 32,678 + LRK53_RS18275 0.11 +0.5 32,678 + LRK53_RS18275 0.11 -0.2 32,678 + LRK53_RS18275 0.11 -0.2 32,678 + LRK53_RS18275 0.11 -2.1 32,678 + LRK53_RS18275 0.11 -1.5 32,679 - LRK53_RS18275 0.11 -0.6 32,679 - LRK53_RS18275 0.11 -0.6 32,679 - LRK53_RS18275 0.11 -0.7 32,679 - LRK53_RS18275 0.11 -0.5 32,691 - LRK53_RS18275 0.13 -0.8 32,793 - LRK53_RS18275 0.30 +0.5 32,793 - LRK53_RS18275 0.30 -1.3 32,905 + LRK53_RS18275 0.49 -1.5 32,905 + LRK53_RS18275 0.49 +1.1 32,905 + LRK53_RS18275 0.49 -0.3 32,906 - LRK53_RS18275 0.49 -0.9 32,906 - LRK53_RS18275 0.49 +0.6 32,906 - LRK53_RS18275 0.49 -0.3 33,416 - LRK53_RS18280 0.23 -1.5 33,416 - LRK53_RS18280 0.23 -1.1 33,416 - LRK53_RS18280 0.23 -0.6 33,416 - LRK53_RS18280 0.23 -0.9 33,416 - LRK53_RS18280 0.23 -1.9 33,416 - LRK53_RS18280 0.23 +0.3 33,416 - LRK53_RS18280 0.23 +0.3 33,535 + LRK53_RS18280 0.39 -0.1 33,536 - LRK53_RS18280 0.39 +0.3 33,536 - LRK53_RS18280 0.39 -1.7 33,575 + LRK53_RS18280 0.44 -1.3 33,576 - LRK53_RS18280 0.44 -0.2 33,576 - LRK53_RS18280 0.44 -0.9 33,576 - LRK53_RS18280 0.44 -1.0 33,577 + LRK53_RS18280 0.44 +1.6 33,577 + LRK53_RS18280 0.44 -1.5 33,577 + LRK53_RS18280 0.44 -0.7 33,577 + LRK53_RS18280 0.44 -1.5 33,577 + LRK53_RS18280 0.44 -1.5 33,577 + LRK53_RS18280 0.44 -1.7 33,577 + LRK53_RS18280 0.44 +1.1 33,577 + LRK53_RS18280 0.44 +1.6 33,577 + LRK53_RS18280 0.44 -0.7 33,577 + LRK53_RS18280 0.44 -0.9 33,577 + LRK53_RS18280 0.44 -2.4 33,577 + LRK53_RS18280 0.44 -0.9 33,577 + LRK53_RS18280 0.44 +0.3 33,577 + LRK53_RS18280 0.44 -1.8 33,577 + LRK53_RS18280 0.44 -0.4 33,577 + LRK53_RS18280 0.44 -1.7 33,577 + LRK53_RS18280 0.44 -0.6 33,578 - LRK53_RS18280 0.44 -0.9 33,578 - LRK53_RS18280 0.44 -0.5 33,578 - LRK53_RS18280 0.44 -2.9 33,578 - LRK53_RS18280 0.44 +0.1 33,578 - LRK53_RS18280 0.44 -0.5 33,578 - LRK53_RS18280 0.44 -0.4 33,578 - LRK53_RS18280 0.44 +1.7 33,578 - LRK53_RS18280 0.44 -2.1 33,578 - LRK53_RS18280 0.44 -0.5 33,578 - LRK53_RS18280 0.44 +0.5 33,578 - LRK53_RS18280 0.44 -0.5 33,578 - LRK53_RS18280 0.44 -0.5 33,578 - LRK53_RS18280 0.44 +0.1 33,578 - LRK53_RS18280 0.44 -1.3 33,578 - LRK53_RS18280 0.44 +1.1 33,578 - LRK53_RS18280 0.44 +0.1 33,760 + LRK53_RS18280 0.69 -0.4 33,769 + LRK53_RS18280 0.70 -0.2 33,769 + LRK53_RS18280 0.70 -1.9 33,805 + LRK53_RS18280 0.75 +0.1 33,806 - LRK53_RS18280 0.75 -1.9 33,806 - LRK53_RS18280 0.75 -0.5 33,806 - LRK53_RS18280 0.75 -1.7 33,811 + LRK53_RS18280 0.75 -1.9 33,811 + LRK53_RS18280 0.75 -1.6 33,811 + LRK53_RS18280 0.75 -0.3 33,812 - LRK53_RS18280 0.76 -0.9 33,812 - LRK53_RS18280 0.76 -0.9 33,812 - LRK53_RS18280 0.76 +1.1 33,812 - LRK53_RS18280 0.76 -1.3 33,845 - LRK53_RS18280 0.80 +0.7 33,845 - LRK53_RS18280 0.80 +0.1 33,845 - LRK53_RS18280 0.80 +0.2 33,845 - LRK53_RS18280 0.80 -1.2 33,845 - LRK53_RS18280 0.80 -1.5 34,017 - -1.7 34,068 + -1.3 34,149 - +0.4 34,194 + -0.3 34,194 + -1.3
Or see this region's nucleotide sequence