Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09260 and LRK53_RS09265 overlap by 4 nucleotides LRK53_RS09265 and LRK53_RS09270 are separated by 3 nucleotides LRK53_RS09270 and LRK53_RS09275 are separated by 251 nucleotides LRK53_RS09275 and LRK53_RS09280 are separated by 55 nucleotides
LRK53_RS09260: LRK53_RS09260 - ABC transporter ATP-binding protein, at 2,070,917 to 2,071,774
_RS09260
LRK53_RS09265: LRK53_RS09265 - GntR family transcriptional regulator, at 2,071,771 to 2,072,145
_RS09265
LRK53_RS09270: LRK53_RS09270 - hypothetical protein, at 2,072,149 to 2,072,490
_RS09270
LRK53_RS09275: LRK53_RS09275 - SDR family oxidoreductase, at 2,072,742 to 2,073,527
_RS09275
LRK53_RS09280: LRK53_RS09280 - amidohydrolase, at 2,073,583 to 2,075,250
_RS09280
Position (kb)
2072
2073
2074 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2071.982 kb on + strand, within LRK53_RS09265 at 2071.982 kb on + strand, within LRK53_RS09265 at 2071.982 kb on + strand, within LRK53_RS09265 at 2071.983 kb on - strand, within LRK53_RS09265 at 2072.111 kb on + strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.150 kb on + strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.190 kb on - strand, within LRK53_RS09270 at 2072.190 kb on - strand, within LRK53_RS09270 at 2072.192 kb on + strand, within LRK53_RS09270 at 2072.192 kb on + strand, within LRK53_RS09270 at 2072.193 kb on - strand, within LRK53_RS09270 at 2072.193 kb on - strand, within LRK53_RS09270 at 2072.262 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.562 kb on - strand at 2072.597 kb on + strand at 2072.597 kb on + strand at 2072.597 kb on + strand at 2072.597 kb on + strand at 2072.597 kb on + strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2073.087 kb on + strand, within LRK53_RS09275 at 2073.087 kb on + strand, within LRK53_RS09275 at 2073.088 kb on - strand, within LRK53_RS09275 at 2073.088 kb on - strand, within LRK53_RS09275 at 2073.088 kb on - strand, within LRK53_RS09275 at 2073.088 kb on - strand, within LRK53_RS09275 at 2073.088 kb on - strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.096 kb on + strand, within LRK53_RS09275 at 2073.097 kb on - strand, within LRK53_RS09275 at 2073.097 kb on - strand, within LRK53_RS09275 at 2073.097 kb on - strand, within LRK53_RS09275 at 2073.097 kb on - strand, within LRK53_RS09275 at 2073.097 kb on - strand, within LRK53_RS09275 at 2073.097 kb on - strand, within LRK53_RS09275 at 2073.249 kb on + strand, within LRK53_RS09275 at 2073.249 kb on + strand, within LRK53_RS09275 at 2073.250 kb on - strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.261 kb on + strand, within LRK53_RS09275 at 2073.262 kb on - strand, within LRK53_RS09275 at 2073.262 kb on - strand, within LRK53_RS09275 at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.474 kb on + strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.475 kb on - strand at 2073.599 kb on + strand at 2073.599 kb on + strand at 2073.599 kb on + strand at 2073.600 kb on - strand at 2073.950 kb on + strand, within LRK53_RS09280 at 2073.951 kb on - strand, within LRK53_RS09280 at 2073.951 kb on - strand, within LRK53_RS09280 at 2074.133 kb on + strand, within LRK53_RS09280 at 2074.133 kb on + strand, within LRK53_RS09280 at 2074.134 kb on - strand, within LRK53_RS09280 at 2074.134 kb on - strand, within LRK53_RS09280 at 2074.134 kb on - strand, within LRK53_RS09280 at 2074.134 kb on - strand, within LRK53_RS09280 at 2074.134 kb on - strand, within LRK53_RS09280 at 2074.145 kb on + strand, within LRK53_RS09280 at 2074.146 kb on - strand, within LRK53_RS09280 at 2074.289 kb on + strand, within LRK53_RS09280 at 2074.290 kb on - strand, within LRK53_RS09280 at 2074.442 kb on + strand, within LRK53_RS09280 at 2074.442 kb on + strand, within LRK53_RS09280 at 2074.443 kb on - strand, within LRK53_RS09280 at 2074.443 kb on - strand, within LRK53_RS09280 at 2074.443 kb on - strand, within LRK53_RS09280 at 2074.446 kb on + strand, within LRK53_RS09280 at 2074.446 kb on + strand, within LRK53_RS09280 at 2074.446 kb on + strand, within LRK53_RS09280 at 2074.446 kb on + strand, within LRK53_RS09280 at 2074.446 kb on + strand, within LRK53_RS09280 at 2074.446 kb on + strand, within LRK53_RS09280 at 2074.446 kb on + strand, within LRK53_RS09280 at 2074.447 kb on - strand, within LRK53_RS09280 at 2074.447 kb on - strand, within LRK53_RS09280 at 2074.475 kb on + strand, within LRK53_RS09280 at 2074.476 kb on - strand, within LRK53_RS09280 at 2074.476 kb on - strand, within LRK53_RS09280 at 2074.476 kb on - strand, within LRK53_RS09280 at 2074.493 kb on + strand, within LRK53_RS09280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 2,071,982 + LRK53_RS09265 0.56 -1.1 2,071,982 + LRK53_RS09265 0.56 -0.7 2,071,982 + LRK53_RS09265 0.56 -0.1 2,071,983 - LRK53_RS09265 0.57 +0.3 2,072,111 + -1.9 2,072,112 - -0.7 2,072,112 - -1.3 2,072,112 - -0.7 2,072,112 - -0.1 2,072,112 - -0.1 2,072,150 + +0.5 2,072,151 - -0.1 2,072,151 - -1.6 2,072,151 - -0.1 2,072,151 - -0.7 2,072,151 - -2.4 2,072,151 - -2.6 2,072,189 + LRK53_RS09270 0.12 -2.1 2,072,189 + LRK53_RS09270 0.12 +0.3 2,072,189 + LRK53_RS09270 0.12 -0.9 2,072,189 + LRK53_RS09270 0.12 -0.7 2,072,189 + LRK53_RS09270 0.12 -0.7 2,072,190 - LRK53_RS09270 0.12 +1.3 2,072,190 - LRK53_RS09270 0.12 +0.9 2,072,192 + LRK53_RS09270 0.13 -0.1 2,072,192 + LRK53_RS09270 0.13 -1.0 2,072,193 - LRK53_RS09270 0.13 +0.9 2,072,193 - LRK53_RS09270 0.13 +0.5 2,072,262 + LRK53_RS09270 0.33 -0.7 2,072,417 + LRK53_RS09270 0.78 -1.7 2,072,417 + LRK53_RS09270 0.78 +0.9 2,072,417 + LRK53_RS09270 0.78 -3.8 2,072,417 + LRK53_RS09270 0.78 -0.4 2,072,417 + LRK53_RS09270 0.78 +0.3 2,072,417 + LRK53_RS09270 0.78 -0.1 2,072,417 + LRK53_RS09270 0.78 -0.1 2,072,418 - LRK53_RS09270 0.79 -0.1 2,072,418 - LRK53_RS09270 0.79 -0.9 2,072,418 - LRK53_RS09270 0.79 +0.3 2,072,418 - LRK53_RS09270 0.79 +0.2 2,072,418 - LRK53_RS09270 0.79 +1.3 2,072,562 - -1.7 2,072,597 + -0.4 2,072,597 + -2.0 2,072,597 + +0.9 2,072,597 + +0.1 2,072,597 + +0.5 2,072,598 - -0.1 2,072,598 - -1.1 2,072,598 - +1.9 2,072,598 - -1.4 2,072,598 - +0.1 2,072,598 - +0.3 2,072,598 - -3.3 2,072,598 - -0.0 2,072,598 - -0.7 2,072,598 - -1.9 2,072,598 - -1.7 2,073,087 + LRK53_RS09275 0.44 -1.1 2,073,087 + LRK53_RS09275 0.44 -0.1 2,073,088 - LRK53_RS09275 0.44 -0.1 2,073,088 - LRK53_RS09275 0.44 -1.3 2,073,088 - LRK53_RS09275 0.44 -3.0 2,073,088 - LRK53_RS09275 0.44 -1.7 2,073,088 - LRK53_RS09275 0.44 +0.3 2,073,096 + LRK53_RS09275 0.45 -0.2 2,073,096 + LRK53_RS09275 0.45 -1.1 2,073,096 + LRK53_RS09275 0.45 -1.1 2,073,096 + LRK53_RS09275 0.45 +0.2 2,073,096 + LRK53_RS09275 0.45 -0.1 2,073,096 + LRK53_RS09275 0.45 -2.4 2,073,096 + LRK53_RS09275 0.45 +0.9 2,073,096 + LRK53_RS09275 0.45 +0.6 2,073,096 + LRK53_RS09275 0.45 -0.4 2,073,096 + LRK53_RS09275 0.45 -2.1 2,073,096 + LRK53_RS09275 0.45 -0.9 2,073,096 + LRK53_RS09275 0.45 -0.7 2,073,096 + LRK53_RS09275 0.45 -0.6 2,073,097 - LRK53_RS09275 0.45 +1.3 2,073,097 - LRK53_RS09275 0.45 -0.7 2,073,097 - LRK53_RS09275 0.45 +1.9 2,073,097 - LRK53_RS09275 0.45 +0.6 2,073,097 - LRK53_RS09275 0.45 -1.1 2,073,097 - LRK53_RS09275 0.45 +0.9 2,073,249 + LRK53_RS09275 0.65 -2.4 2,073,249 + LRK53_RS09275 0.65 -2.1 2,073,250 - LRK53_RS09275 0.65 -2.1 2,073,261 + LRK53_RS09275 0.66 -1.4 2,073,261 + LRK53_RS09275 0.66 +0.9 2,073,261 + LRK53_RS09275 0.66 -1.4 2,073,261 + LRK53_RS09275 0.66 -0.2 2,073,261 + LRK53_RS09275 0.66 -0.1 2,073,261 + LRK53_RS09275 0.66 -0.1 2,073,261 + LRK53_RS09275 0.66 -1.1 2,073,261 + LRK53_RS09275 0.66 +0.3 2,073,262 - LRK53_RS09275 0.66 +0.3 2,073,262 - LRK53_RS09275 0.66 -0.7 2,073,474 + +0.9 2,073,474 + +0.9 2,073,474 + -0.1 2,073,474 + -0.3 2,073,474 + -0.7 2,073,474 + -2.1 2,073,474 + -0.7 2,073,474 + -0.4 2,073,474 + +0.9 2,073,474 + -0.3 2,073,474 + +0.9 2,073,474 + +0.3 2,073,474 + +0.9 2,073,474 + +1.9 2,073,474 + +0.6 2,073,474 + +1.9 2,073,474 + +1.5 2,073,474 + -1.7 2,073,474 + -0.5 2,073,475 - +0.9 2,073,475 - +0.9 2,073,475 - -0.1 2,073,475 - +0.1 2,073,475 - +1.2 2,073,475 - -0.3 2,073,475 - +0.1 2,073,475 - -0.7 2,073,475 - -1.1 2,073,475 - -0.1 2,073,475 - -0.1 2,073,475 - +0.9 2,073,475 - +0.1 2,073,475 - +0.9 2,073,475 - -0.7 2,073,475 - -1.1 2,073,475 - -1.4 2,073,475 - +0.9 2,073,475 - -1.7 2,073,599 + -0.7 2,073,599 + -1.1 2,073,599 + -1.7 2,073,600 - -0.9 2,073,950 + LRK53_RS09280 0.22 +0.6 2,073,951 - LRK53_RS09280 0.22 +0.4 2,073,951 - LRK53_RS09280 0.22 -0.7 2,074,133 + LRK53_RS09280 0.33 -0.6 2,074,133 + LRK53_RS09280 0.33 +2.5 2,074,134 - LRK53_RS09280 0.33 -0.3 2,074,134 - LRK53_RS09280 0.33 -1.1 2,074,134 - LRK53_RS09280 0.33 -2.3 2,074,134 - LRK53_RS09280 0.33 -0.1 2,074,134 - LRK53_RS09280 0.33 -2.1 2,074,145 + LRK53_RS09280 0.34 -0.7 2,074,146 - LRK53_RS09280 0.34 -0.4 2,074,289 + LRK53_RS09280 0.42 +0.3 2,074,290 - LRK53_RS09280 0.42 -1.4 2,074,442 + LRK53_RS09280 0.51 -0.7 2,074,442 + LRK53_RS09280 0.51 -1.4 2,074,443 - LRK53_RS09280 0.52 -1.0 2,074,443 - LRK53_RS09280 0.52 -0.7 2,074,443 - LRK53_RS09280 0.52 -0.5 2,074,446 + LRK53_RS09280 0.52 -0.4 2,074,446 + LRK53_RS09280 0.52 -0.7 2,074,446 + LRK53_RS09280 0.52 +0.9 2,074,446 + LRK53_RS09280 0.52 -1.1 2,074,446 + LRK53_RS09280 0.52 -0.3 2,074,446 + LRK53_RS09280 0.52 -1.1 2,074,446 + LRK53_RS09280 0.52 +0.1 2,074,447 - LRK53_RS09280 0.52 -0.7 2,074,447 - LRK53_RS09280 0.52 -1.4 2,074,475 + LRK53_RS09280 0.53 +2.1 2,074,476 - LRK53_RS09280 0.54 -1.4 2,074,476 - LRK53_RS09280 0.54 -1.9 2,074,476 - LRK53_RS09280 0.54 +0.3 2,074,493 + LRK53_RS09280 0.55 -0.7
Or see this region's nucleotide sequence