Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05245 and LRK53_RS05250 are separated by 158 nucleotides LRK53_RS05250 and LRK53_RS05255 are separated by 128 nucleotides
LRK53_RS05245: LRK53_RS05245 - Fe-S oxidoreductase, at 1,227,739 to 1,229,082
_RS05245
LRK53_RS05250: LRK53_RS05250 - rubrerythrin family protein, at 1,229,241 to 1,229,660
_RS05250
LRK53_RS05255: LRK53_RS05255 - LysR substrate-binding domain-containing protein, at 1,229,789 to 1,230,742
_RS05255
Position (kb)
1229
1230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1228.289 kb on + strand, within LRK53_RS05245 at 1228.289 kb on + strand, within LRK53_RS05245 at 1228.289 kb on + strand, within LRK53_RS05245 at 1228.289 kb on + strand, within LRK53_RS05245 at 1228.289 kb on + strand, within LRK53_RS05245 at 1228.289 kb on + strand, within LRK53_RS05245 at 1228.289 kb on + strand, within LRK53_RS05245 at 1228.290 kb on - strand, within LRK53_RS05245 at 1228.290 kb on - strand, within LRK53_RS05245 at 1228.290 kb on - strand, within LRK53_RS05245 at 1228.290 kb on - strand, within LRK53_RS05245 at 1228.290 kb on - strand, within LRK53_RS05245 at 1228.290 kb on - strand, within LRK53_RS05245 at 1228.290 kb on - strand, within LRK53_RS05245 at 1228.433 kb on + strand, within LRK53_RS05245 at 1228.433 kb on + strand, within LRK53_RS05245 at 1228.433 kb on + strand, within LRK53_RS05245 at 1228.538 kb on + strand, within LRK53_RS05245 at 1228.539 kb on - strand, within LRK53_RS05245 at 1228.539 kb on - strand, within LRK53_RS05245 at 1228.779 kb on + strand, within LRK53_RS05245 at 1228.780 kb on - strand, within LRK53_RS05245 at 1228.780 kb on - strand, within LRK53_RS05245 at 1228.780 kb on - strand, within LRK53_RS05245 at 1228.780 kb on - strand, within LRK53_RS05245 at 1228.781 kb on + strand, within LRK53_RS05245 at 1228.781 kb on + strand, within LRK53_RS05245 at 1228.782 kb on - strand, within LRK53_RS05245 at 1228.782 kb on - strand, within LRK53_RS05245 at 1228.782 kb on - strand, within LRK53_RS05245 at 1228.799 kb on + strand, within LRK53_RS05245 at 1228.799 kb on + strand, within LRK53_RS05245 at 1228.817 kb on + strand, within LRK53_RS05245 at 1228.817 kb on + strand, within LRK53_RS05245 at 1228.817 kb on + strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1228.818 kb on - strand, within LRK53_RS05245 at 1229.035 kb on - strand at 1229.084 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.085 kb on + strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.086 kb on - strand at 1229.179 kb on - strand at 1229.368 kb on + strand, within LRK53_RS05250 at 1229.368 kb on + strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.369 kb on - strand, within LRK53_RS05250 at 1229.380 kb on + strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.381 kb on - strand, within LRK53_RS05250 at 1229.474 kb on - strand, within LRK53_RS05250 at 1229.474 kb on - strand, within LRK53_RS05250 at 1229.545 kb on + strand, within LRK53_RS05250 at 1229.545 kb on + strand, within LRK53_RS05250 at 1229.545 kb on + strand, within LRK53_RS05250 at 1229.545 kb on + strand, within LRK53_RS05250 at 1229.545 kb on + strand, within LRK53_RS05250 at 1229.545 kb on + strand, within LRK53_RS05250 at 1229.546 kb on - strand, within LRK53_RS05250 at 1229.546 kb on - strand, within LRK53_RS05250 at 1229.546 kb on - strand, within LRK53_RS05250 at 1229.546 kb on - strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.575 kb on + strand, within LRK53_RS05250 at 1229.576 kb on - strand, within LRK53_RS05250 at 1229.576 kb on - strand, within LRK53_RS05250 at 1229.576 kb on - strand, within LRK53_RS05250 at 1229.576 kb on - strand, within LRK53_RS05250 at 1229.576 kb on - strand, within LRK53_RS05250 at 1229.576 kb on - strand, within LRK53_RS05250 at 1229.576 kb on - strand, within LRK53_RS05250 at 1229.582 kb on - strand, within LRK53_RS05250 at 1229.732 kb on - strand at 1229.732 kb on - strand at 1229.780 kb on - strand at 1229.780 kb on - strand at 1229.780 kb on - strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.873 kb on + strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1229.874 kb on - strand at 1230.432 kb on + strand, within LRK53_RS05255 at 1230.432 kb on + strand, within LRK53_RS05255 at 1230.432 kb on + strand, within LRK53_RS05255 at 1230.432 kb on + strand, within LRK53_RS05255 at 1230.432 kb on + strand, within LRK53_RS05255 at 1230.432 kb on + strand, within LRK53_RS05255 at 1230.433 kb on - strand, within LRK53_RS05255 at 1230.433 kb on - strand, within LRK53_RS05255 at 1230.433 kb on - strand, within LRK53_RS05255 at 1230.433 kb on - strand, within LRK53_RS05255 at 1230.433 kb on - strand, within LRK53_RS05255 at 1230.433 kb on - strand, within LRK53_RS05255 at 1230.433 kb on - strand, within LRK53_RS05255 at 1230.440 kb on + strand, within LRK53_RS05255 at 1230.440 kb on + strand, within LRK53_RS05255 at 1230.440 kb on + strand, within LRK53_RS05255 at 1230.440 kb on + strand, within LRK53_RS05255 at 1230.440 kb on + strand, within LRK53_RS05255 at 1230.440 kb on + strand, within LRK53_RS05255 at 1230.440 kb on + strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255 at 1230.441 kb on - strand, within LRK53_RS05255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 1,228,289 + LRK53_RS05245 0.41 +0.9 1,228,289 + LRK53_RS05245 0.41 -0.4 1,228,289 + LRK53_RS05245 0.41 -3.3 1,228,289 + LRK53_RS05245 0.41 -1.9 1,228,289 + LRK53_RS05245 0.41 +1.9 1,228,289 + LRK53_RS05245 0.41 -0.7 1,228,289 + LRK53_RS05245 0.41 -0.7 1,228,290 - LRK53_RS05245 0.41 -0.4 1,228,290 - LRK53_RS05245 0.41 +0.9 1,228,290 - LRK53_RS05245 0.41 +0.3 1,228,290 - LRK53_RS05245 0.41 +0.3 1,228,290 - LRK53_RS05245 0.41 +0.0 1,228,290 - LRK53_RS05245 0.41 -2.7 1,228,290 - LRK53_RS05245 0.41 -2.4 1,228,433 + LRK53_RS05245 0.52 +0.9 1,228,433 + LRK53_RS05245 0.52 +0.9 1,228,433 + LRK53_RS05245 0.52 -0.7 1,228,538 + LRK53_RS05245 0.59 +0.2 1,228,539 - LRK53_RS05245 0.60 -0.1 1,228,539 - LRK53_RS05245 0.60 +0.0 1,228,779 + LRK53_RS05245 0.77 -0.4 1,228,780 - LRK53_RS05245 0.77 -1.1 1,228,780 - LRK53_RS05245 0.77 -0.4 1,228,780 - LRK53_RS05245 0.77 -2.3 1,228,780 - LRK53_RS05245 0.77 -0.4 1,228,781 + LRK53_RS05245 0.78 -0.1 1,228,781 + LRK53_RS05245 0.78 +0.9 1,228,782 - LRK53_RS05245 0.78 +0.9 1,228,782 - LRK53_RS05245 0.78 -1.4 1,228,782 - LRK53_RS05245 0.78 -0.9 1,228,799 + LRK53_RS05245 0.79 -1.3 1,228,799 + LRK53_RS05245 0.79 -0.1 1,228,817 + LRK53_RS05245 0.80 -1.7 1,228,817 + LRK53_RS05245 0.80 -0.5 1,228,817 + LRK53_RS05245 0.80 -0.9 1,228,818 - LRK53_RS05245 0.80 -2.9 1,228,818 - LRK53_RS05245 0.80 -1.4 1,228,818 - LRK53_RS05245 0.80 -0.1 1,228,818 - LRK53_RS05245 0.80 -0.4 1,228,818 - LRK53_RS05245 0.80 -1.0 1,228,818 - LRK53_RS05245 0.80 -0.2 1,228,818 - LRK53_RS05245 0.80 +0.9 1,228,818 - LRK53_RS05245 0.80 -1.1 1,229,035 - -0.8 1,229,084 + +1.9 1,229,085 + -0.7 1,229,085 + +1.9 1,229,085 + -2.3 1,229,085 + +1.9 1,229,085 + -2.4 1,229,085 + -1.9 1,229,085 + -0.2 1,229,085 + -2.1 1,229,085 + -0.3 1,229,085 + -2.2 1,229,085 + +0.9 1,229,085 + -0.7 1,229,086 - -1.1 1,229,086 - -0.3 1,229,086 - -0.1 1,229,086 - -0.7 1,229,086 - -2.3 1,229,086 - +0.2 1,229,086 - -2.0 1,229,086 - -1.4 1,229,086 - +0.9 1,229,086 - +0.2 1,229,086 - -1.5 1,229,086 - -0.4 1,229,086 - -0.1 1,229,086 - -1.4 1,229,086 - -1.1 1,229,179 - -0.6 1,229,368 + LRK53_RS05250 0.30 -1.1 1,229,368 + LRK53_RS05250 0.30 -1.1 1,229,369 - LRK53_RS05250 0.30 -0.4 1,229,369 - LRK53_RS05250 0.30 +0.9 1,229,369 - LRK53_RS05250 0.30 +0.9 1,229,369 - LRK53_RS05250 0.30 -0.1 1,229,369 - LRK53_RS05250 0.30 +0.1 1,229,369 - LRK53_RS05250 0.30 -0.2 1,229,369 - LRK53_RS05250 0.30 +0.5 1,229,369 - LRK53_RS05250 0.30 -0.2 1,229,369 - LRK53_RS05250 0.30 +0.9 1,229,369 - LRK53_RS05250 0.30 +0.3 1,229,369 - LRK53_RS05250 0.30 -0.7 1,229,380 + LRK53_RS05250 0.33 -2.0 1,229,381 - LRK53_RS05250 0.33 +0.3 1,229,381 - LRK53_RS05250 0.33 +0.9 1,229,381 - LRK53_RS05250 0.33 +1.2 1,229,381 - LRK53_RS05250 0.33 +2.3 1,229,381 - LRK53_RS05250 0.33 -1.3 1,229,381 - LRK53_RS05250 0.33 -0.2 1,229,381 - LRK53_RS05250 0.33 +0.6 1,229,381 - LRK53_RS05250 0.33 -1.4 1,229,381 - LRK53_RS05250 0.33 +0.9 1,229,381 - LRK53_RS05250 0.33 -1.1 1,229,474 - LRK53_RS05250 0.55 -0.9 1,229,474 - LRK53_RS05250 0.55 -1.7 1,229,545 + LRK53_RS05250 0.72 -1.4 1,229,545 + LRK53_RS05250 0.72 -0.2 1,229,545 + LRK53_RS05250 0.72 -0.3 1,229,545 + LRK53_RS05250 0.72 -0.4 1,229,545 + LRK53_RS05250 0.72 -2.3 1,229,545 + LRK53_RS05250 0.72 +0.3 1,229,546 - LRK53_RS05250 0.73 +0.9 1,229,546 - LRK53_RS05250 0.73 -2.1 1,229,546 - LRK53_RS05250 0.73 -1.1 1,229,546 - LRK53_RS05250 0.73 +0.9 1,229,575 + LRK53_RS05250 0.80 -0.7 1,229,575 + LRK53_RS05250 0.80 -1.9 1,229,575 + LRK53_RS05250 0.80 -1.1 1,229,575 + LRK53_RS05250 0.80 -1.1 1,229,575 + LRK53_RS05250 0.80 -1.4 1,229,575 + LRK53_RS05250 0.80 -0.9 1,229,575 + LRK53_RS05250 0.80 +0.9 1,229,575 + LRK53_RS05250 0.80 -0.2 1,229,575 + LRK53_RS05250 0.80 -0.1 1,229,576 - LRK53_RS05250 0.80 +0.9 1,229,576 - LRK53_RS05250 0.80 -1.4 1,229,576 - LRK53_RS05250 0.80 +0.9 1,229,576 - LRK53_RS05250 0.80 -0.3 1,229,576 - LRK53_RS05250 0.80 +0.9 1,229,576 - LRK53_RS05250 0.80 +0.9 1,229,576 - LRK53_RS05250 0.80 +0.9 1,229,582 - LRK53_RS05250 0.81 -1.1 1,229,732 - -1.1 1,229,732 - -0.4 1,229,780 - -1.1 1,229,780 - +0.9 1,229,780 - -0.7 1,229,873 + -1.7 1,229,873 + -0.5 1,229,873 + +1.9 1,229,873 + -0.9 1,229,873 + -1.1 1,229,873 + -0.5 1,229,873 + -1.8 1,229,873 + -0.0 1,229,873 + -1.1 1,229,874 - -0.6 1,229,874 - -0.2 1,229,874 - -2.1 1,229,874 - +0.9 1,229,874 - -2.9 1,229,874 - -1.5 1,229,874 - +0.2 1,229,874 - -0.8 1,229,874 - -3.0 1,230,432 + LRK53_RS05255 0.67 +0.9 1,230,432 + LRK53_RS05255 0.67 -0.7 1,230,432 + LRK53_RS05255 0.67 -0.4 1,230,432 + LRK53_RS05255 0.67 +0.9 1,230,432 + LRK53_RS05255 0.67 -1.4 1,230,432 + LRK53_RS05255 0.67 -1.3 1,230,433 - LRK53_RS05255 0.68 -1.7 1,230,433 - LRK53_RS05255 0.68 -1.7 1,230,433 - LRK53_RS05255 0.68 -0.7 1,230,433 - LRK53_RS05255 0.68 +0.5 1,230,433 - LRK53_RS05255 0.68 -0.4 1,230,433 - LRK53_RS05255 0.68 -0.7 1,230,433 - LRK53_RS05255 0.68 -0.3 1,230,440 + LRK53_RS05255 0.68 -0.7 1,230,440 + LRK53_RS05255 0.68 -1.4 1,230,440 + LRK53_RS05255 0.68 -3.0 1,230,440 + LRK53_RS05255 0.68 -0.7 1,230,440 + LRK53_RS05255 0.68 -0.7 1,230,440 + LRK53_RS05255 0.68 +0.9 1,230,440 + LRK53_RS05255 0.68 +0.9 1,230,441 - LRK53_RS05255 0.68 +0.9 1,230,441 - LRK53_RS05255 0.68 -0.7 1,230,441 - LRK53_RS05255 0.68 -1.5 1,230,441 - LRK53_RS05255 0.68 -0.4 1,230,441 - LRK53_RS05255 0.68 +0.1 1,230,441 - LRK53_RS05255 0.68 -1.7 1,230,441 - LRK53_RS05255 0.68 +1.9 1,230,441 - LRK53_RS05255 0.68 -0.1 1,230,441 - LRK53_RS05255 0.68 -0.4 1,230,441 - LRK53_RS05255 0.68 -0.4
Or see this region's nucleotide sequence