Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04140 and LRK53_RS04145 are separated by 158 nucleotides LRK53_RS04145 and LRK53_RS04150 are separated by 67 nucleotides LRK53_RS04150 and LRK53_RS04155 are separated by 97 nucleotides
LRK53_RS04140: LRK53_RS04140 - hypothetical protein, at 968,835 to 969,293
_RS04140
LRK53_RS04145: LRK53_RS04145 - hypothetical protein, at 969,452 to 969,925
_RS04145
LRK53_RS04150: LRK53_RS04150 - DUF2884 family protein, at 969,993 to 970,745
_RS04150
LRK53_RS04155: LRK53_RS04155 - DUF2383 domain-containing protein, at 970,843 to 971,283
_RS04155
Position (kb) 969
970 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 968.552 kb on + strand at 968.552 kb on + strand at 968.553 kb on - strand at 968.619 kb on + strand at 968.620 kb on - strand at 968.620 kb on - strand at 968.628 kb on + strand at 968.628 kb on + strand at 968.640 kb on - strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.657 kb on + strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.658 kb on - strand at 968.787 kb on + strand at 968.787 kb on + strand at 968.787 kb on + strand at 968.787 kb on + strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.788 kb on - strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.801 kb on + strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.846 kb on + strand at 968.846 kb on + strand at 968.846 kb on + strand at 968.846 kb on + strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.847 kb on - strand at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.952 kb on + strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.953 kb on - strand, within LRK53_RS04140 at 968.979 kb on + strand, within LRK53_RS04140 at 968.979 kb on + strand, within LRK53_RS04140 at 968.979 kb on + strand, within LRK53_RS04140 at 968.980 kb on - strand, within LRK53_RS04140 at 968.980 kb on - strand, within LRK53_RS04140 at 968.980 kb on - strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.078 kb on + strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140 at 969.079 kb on - strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.135 kb on + strand, within LRK53_RS04140 at 969.136 kb on - strand, within LRK53_RS04140 at 969.136 kb on - strand, within LRK53_RS04140 at 969.136 kb on - strand, within LRK53_RS04140 at 969.136 kb on - strand, within LRK53_RS04140 at 969.136 kb on - strand, within LRK53_RS04140 at 969.136 kb on - strand, within LRK53_RS04140 at 969.144 kb on + strand, within LRK53_RS04140 at 969.144 kb on + strand, within LRK53_RS04140 at 969.144 kb on + strand, within LRK53_RS04140 at 969.144 kb on + strand, within LRK53_RS04140 at 969.144 kb on + strand, within LRK53_RS04140 at 969.144 kb on + strand, within LRK53_RS04140 at 969.144 kb on + strand, within LRK53_RS04140 at 969.145 kb on - strand, within LRK53_RS04140 at 969.145 kb on - strand, within LRK53_RS04140 at 969.350 kb on + strand at 969.350 kb on + strand at 969.351 kb on - strand at 969.351 kb on - strand at 969.557 kb on + strand, within LRK53_RS04145 at 969.557 kb on + strand, within LRK53_RS04145 at 969.557 kb on + strand, within LRK53_RS04145 at 969.557 kb on + strand, within LRK53_RS04145 at 969.557 kb on + strand, within LRK53_RS04145 at 969.557 kb on + strand, within LRK53_RS04145 at 969.558 kb on - strand, within LRK53_RS04145 at 969.558 kb on - strand, within LRK53_RS04145 at 970.092 kb on + strand, within LRK53_RS04150 at 970.092 kb on + strand, within LRK53_RS04150 at 970.092 kb on + strand, within LRK53_RS04150 at 970.092 kb on + strand, within LRK53_RS04150 at 970.092 kb on + strand, within LRK53_RS04150 at 970.092 kb on + strand, within LRK53_RS04150 at 970.092 kb on + strand, within LRK53_RS04150 at 970.092 kb on + strand, within LRK53_RS04150 at 970.093 kb on - strand, within LRK53_RS04150 at 970.093 kb on - strand, within LRK53_RS04150 at 970.093 kb on - strand, within LRK53_RS04150 at 970.093 kb on - strand, within LRK53_RS04150 at 970.409 kb on + strand, within LRK53_RS04150 at 970.409 kb on + strand, within LRK53_RS04150 at 970.409 kb on + strand, within LRK53_RS04150 at 970.409 kb on + strand, within LRK53_RS04150 at 970.409 kb on + strand, within LRK53_RS04150 at 970.409 kb on + strand, within LRK53_RS04150 at 970.410 kb on - strand, within LRK53_RS04150 at 970.410 kb on - strand, within LRK53_RS04150 at 970.793 kb on + strand at 970.793 kb on + strand at 970.793 kb on + strand at 970.793 kb on + strand at 970.793 kb on + strand at 970.794 kb on - strand at 970.794 kb on - strand at 970.794 kb on - strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.860 kb on + strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand at 970.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 968,552 + -0.6 968,552 + -2.6 968,553 - -1.2 968,619 + +0.9 968,620 - -2.1 968,620 - +0.9 968,628 + +1.4 968,628 + -0.1 968,640 - -0.4 968,657 + +0.9 968,657 + -1.4 968,657 + +0.5 968,657 + -0.9 968,657 + +0.9 968,658 - -0.1 968,658 - -2.8 968,658 - -0.1 968,658 - -0.6 968,658 - +0.4 968,658 - -1.3 968,658 - -0.6 968,787 + -1.6 968,787 + -1.9 968,787 + -1.4 968,787 + +1.9 968,788 - +0.5 968,788 - -0.8 968,788 - -0.4 968,788 - -0.6 968,788 - -0.6 968,788 - -1.6 968,788 - -1.0 968,801 + -0.1 968,801 + +0.5 968,801 + -0.1 968,801 + -0.6 968,801 + +1.4 968,801 + +0.4 968,801 + -0.6 968,801 + +0.4 968,801 + -1.1 968,801 + +0.1 968,801 + -0.6 968,801 + +0.9 968,801 + -1.9 968,801 + -0.1 968,802 - +0.6 968,802 - +0.9 968,802 - -0.1 968,802 - -0.4 968,802 - -2.6 968,802 - -0.1 968,802 - -0.1 968,802 - -2.1 968,802 - -0.1 968,802 - -1.4 968,802 - -0.1 968,802 - -1.2 968,802 - -0.9 968,802 - -1.6 968,802 - -1.1 968,802 - -1.4 968,846 + -0.1 968,846 + +0.9 968,846 + -1.1 968,846 + -0.1 968,847 - -0.4 968,847 - -0.1 968,847 - -1.0 968,847 - -0.6 968,847 - -1.6 968,847 - -0.6 968,847 - -0.7 968,847 - -1.5 968,952 + LRK53_RS04140 0.25 -1.5 968,952 + LRK53_RS04140 0.25 +0.7 968,952 + LRK53_RS04140 0.25 -3.2 968,952 + LRK53_RS04140 0.25 -1.7 968,952 + LRK53_RS04140 0.25 -2.7 968,952 + LRK53_RS04140 0.25 -0.5 968,952 + LRK53_RS04140 0.25 -1.9 968,952 + LRK53_RS04140 0.25 -0.6 968,952 + LRK53_RS04140 0.25 -1.2 968,952 + LRK53_RS04140 0.25 -0.1 968,952 + LRK53_RS04140 0.25 -1.1 968,952 + LRK53_RS04140 0.25 +1.5 968,952 + LRK53_RS04140 0.25 +0.9 968,952 + LRK53_RS04140 0.25 -1.2 968,953 - LRK53_RS04140 0.26 -0.4 968,953 - LRK53_RS04140 0.26 -1.4 968,953 - LRK53_RS04140 0.26 -2.5 968,953 - LRK53_RS04140 0.26 -0.5 968,953 - LRK53_RS04140 0.26 +0.7 968,953 - LRK53_RS04140 0.26 +0.9 968,953 - LRK53_RS04140 0.26 -0.1 968,953 - LRK53_RS04140 0.26 +0.9 968,953 - LRK53_RS04140 0.26 -1.1 968,953 - LRK53_RS04140 0.26 +0.6 968,979 + LRK53_RS04140 0.31 -3.6 968,979 + LRK53_RS04140 0.31 -1.6 968,979 + LRK53_RS04140 0.31 -1.9 968,980 - LRK53_RS04140 0.32 -3.1 968,980 - LRK53_RS04140 0.32 -1.1 968,980 - LRK53_RS04140 0.32 -1.6 969,078 + LRK53_RS04140 0.53 +0.4 969,078 + LRK53_RS04140 0.53 +1.9 969,078 + LRK53_RS04140 0.53 -0.6 969,078 + LRK53_RS04140 0.53 +0.9 969,078 + LRK53_RS04140 0.53 -1.1 969,078 + LRK53_RS04140 0.53 -0.4 969,078 + LRK53_RS04140 0.53 -1.6 969,079 - LRK53_RS04140 0.53 +1.9 969,079 - LRK53_RS04140 0.53 +0.9 969,079 - LRK53_RS04140 0.53 -1.6 969,079 - LRK53_RS04140 0.53 +0.5 969,135 + LRK53_RS04140 0.65 -0.1 969,135 + LRK53_RS04140 0.65 -1.4 969,135 + LRK53_RS04140 0.65 -1.1 969,135 + LRK53_RS04140 0.65 -0.1 969,135 + LRK53_RS04140 0.65 -1.1 969,135 + LRK53_RS04140 0.65 +1.9 969,135 + LRK53_RS04140 0.65 -0.1 969,135 + LRK53_RS04140 0.65 -1.4 969,135 + LRK53_RS04140 0.65 +0.9 969,136 - LRK53_RS04140 0.66 -1.2 969,136 - LRK53_RS04140 0.66 +0.3 969,136 - LRK53_RS04140 0.66 +0.5 969,136 - LRK53_RS04140 0.66 +0.9 969,136 - LRK53_RS04140 0.66 +0.9 969,136 - LRK53_RS04140 0.66 -2.5 969,144 + LRK53_RS04140 0.67 -0.6 969,144 + LRK53_RS04140 0.67 -1.6 969,144 + LRK53_RS04140 0.67 +0.9 969,144 + LRK53_RS04140 0.67 -0.5 969,144 + LRK53_RS04140 0.67 -0.6 969,144 + LRK53_RS04140 0.67 -0.6 969,144 + LRK53_RS04140 0.67 +0.9 969,145 - LRK53_RS04140 0.68 -0.7 969,145 - LRK53_RS04140 0.68 -0.9 969,350 + -0.6 969,350 + -0.1 969,351 - +0.4 969,351 - +0.4 969,557 + LRK53_RS04145 0.22 -0.1 969,557 + LRK53_RS04145 0.22 +0.9 969,557 + LRK53_RS04145 0.22 -1.2 969,557 + LRK53_RS04145 0.22 +0.9 969,557 + LRK53_RS04145 0.22 -2.1 969,557 + LRK53_RS04145 0.22 -0.3 969,558 - LRK53_RS04145 0.22 +0.3 969,558 - LRK53_RS04145 0.22 -3.1 970,092 + LRK53_RS04150 0.13 -0.1 970,092 + LRK53_RS04150 0.13 -2.7 970,092 + LRK53_RS04150 0.13 +0.9 970,092 + LRK53_RS04150 0.13 +0.1 970,092 + LRK53_RS04150 0.13 -1.7 970,092 + LRK53_RS04150 0.13 +0.0 970,092 + LRK53_RS04150 0.13 -0.1 970,092 + LRK53_RS04150 0.13 -1.2 970,093 - LRK53_RS04150 0.13 -0.9 970,093 - LRK53_RS04150 0.13 +0.9 970,093 - LRK53_RS04150 0.13 -0.1 970,093 - LRK53_RS04150 0.13 -0.1 970,409 + LRK53_RS04150 0.55 -1.1 970,409 + LRK53_RS04150 0.55 -1.1 970,409 + LRK53_RS04150 0.55 -1.2 970,409 + LRK53_RS04150 0.55 -2.5 970,409 + LRK53_RS04150 0.55 -0.4 970,409 + LRK53_RS04150 0.55 -1.4 970,410 - LRK53_RS04150 0.55 -0.1 970,410 - LRK53_RS04150 0.55 +0.2 970,793 + -2.8 970,793 + -0.3 970,793 + -0.5 970,793 + -0.1 970,793 + +0.1 970,794 - -0.1 970,794 - -1.4 970,794 - -0.1 970,860 + -0.1 970,860 + -0.1 970,860 + +0.9 970,860 + +0.5 970,860 + -1.4 970,860 + -0.1 970,860 + +1.3 970,860 + -0.1 970,860 + -0.7 970,860 + -2.1 970,861 - -0.5 970,861 - +0.3 970,861 - +0.9 970,861 - -0.7 970,861 - -1.1 970,861 - -1.2 970,861 - -1.3 970,861 - +1.7 970,861 - +1.9 970,861 - -1.6 970,861 - +0.1 970,861 - -1.1 970,861 - +0.6 970,861 - -0.5
Or see this region's nucleotide sequence