Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00945 and LRK53_RS00950 overlap by 8 nucleotides LRK53_RS00950 and LRK53_RS00955 are separated by 276 nucleotides LRK53_RS00955 and LRK53_RS00960 are separated by 155 nucleotides
LRK53_RS00945: LRK53_RS00945 - helix-turn-helix domain-containing protein, at 236,945 to 238,063
_RS00945
LRK53_RS00950: LRK53_RS00950 - hypothetical protein, at 238,056 to 238,331
_RS00950
LRK53_RS00955: LRK53_RS00955 - IS200/IS605 family transposase, at 238,608 to 239,039
_RS00955
LRK53_RS00960: LRK53_RS00960 - hypothetical protein, at 239,195 to 242,539
_RS00960
Position (kb) 238
239 Strain fitness (log2 ratio) -2
-1
0
1 at 237.112 kb on + strand, within LRK53_RS00945 at 237.113 kb on - strand, within LRK53_RS00945 at 237.126 kb on - strand, within LRK53_RS00945 at 237.126 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.309 kb on - strand, within LRK53_RS00945 at 238.067 kb on - strand at 238.067 kb on - strand at 238.067 kb on - strand at 238.067 kb on - strand at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.288 kb on + strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.289 kb on - strand, within LRK53_RS00950 at 238.314 kb on + strand at 238.314 kb on + strand at 238.315 kb on - strand at 238.402 kb on + strand at 238.403 kb on - strand at 238.403 kb on - strand at 238.412 kb on + strand at 238.423 kb on + strand at 238.487 kb on - strand at 238.487 kb on - strand at 238.487 kb on - strand at 239.119 kb on + strand at 239.119 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.144 kb on + strand at 239.178 kb on + strand at 239.179 kb on - strand at 239.276 kb on + strand at 239.276 kb on + strand at 239.276 kb on + strand at 239.276 kb on + strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand at 239.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 237,112 + LRK53_RS00945 0.15 -0.9 237,113 - LRK53_RS00945 0.15 -0.9 237,126 - LRK53_RS00945 0.16 -2.5 237,126 - LRK53_RS00945 0.16 -0.5 237,207 - LRK53_RS00945 0.23 -2.2 237,207 - LRK53_RS00945 0.23 -0.9 237,207 - LRK53_RS00945 0.23 +0.5 237,207 - LRK53_RS00945 0.23 -0.9 237,207 - LRK53_RS00945 0.23 +1.5 237,309 - LRK53_RS00945 0.33 -0.5 238,067 - -0.1 238,067 - -0.2 238,067 - -1.7 238,067 - +1.1 238,288 + LRK53_RS00950 0.84 -0.0 238,288 + LRK53_RS00950 0.84 -0.5 238,288 + LRK53_RS00950 0.84 -1.1 238,288 + LRK53_RS00950 0.84 -1.2 238,288 + LRK53_RS00950 0.84 -0.7 238,288 + LRK53_RS00950 0.84 -0.6 238,288 + LRK53_RS00950 0.84 -0.5 238,288 + LRK53_RS00950 0.84 -1.9 238,288 + LRK53_RS00950 0.84 -0.5 238,288 + LRK53_RS00950 0.84 +0.1 238,288 + LRK53_RS00950 0.84 -2.6 238,288 + LRK53_RS00950 0.84 -0.9 238,288 + LRK53_RS00950 0.84 -1.1 238,289 - LRK53_RS00950 0.84 -0.2 238,289 - LRK53_RS00950 0.84 +0.1 238,289 - LRK53_RS00950 0.84 -0.5 238,289 - LRK53_RS00950 0.84 -0.5 238,289 - LRK53_RS00950 0.84 -2.0 238,289 - LRK53_RS00950 0.84 -1.1 238,289 - LRK53_RS00950 0.84 -0.3 238,289 - LRK53_RS00950 0.84 -1.0 238,314 + -1.2 238,314 + +0.7 238,315 - +0.1 238,402 + +0.1 238,403 - -0.5 238,403 - -0.5 238,412 + +0.1 238,423 + +1.1 238,487 - +1.7 238,487 - -0.9 238,487 - -1.2 239,119 + -0.0 239,119 + -0.5 239,144 + -1.2 239,144 + -0.7 239,144 + -0.3 239,144 + +0.0 239,144 + -1.5 239,144 + -0.3 239,144 + -0.2 239,144 + -0.5 239,144 + -2.4 239,144 + -0.5 239,178 + +1.1 239,179 - -0.5 239,276 + +1.1 239,276 + -1.9 239,276 + +0.5 239,276 + -1.9 239,277 - -0.2 239,277 - -1.0 239,277 - +0.1 239,277 - -1.2 239,277 - -0.5 239,277 - -1.5 239,277 - -2.7 239,277 - -1.7 239,277 - -0.2 239,277 - -1.8 239,277 - +1.7 239,277 - +0.1 239,277 - -0.9 239,277 - -0.6
Or see this region's nucleotide sequence