Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18290 and LRK53_RS18295 are separated by 250 nucleotides LRK53_RS18295 and LRK53_RS18300 are separated by 86 nucleotides LRK53_RS18300 and LRK53_RS18305 are separated by 2 nucleotides
LRK53_RS18290: LRK53_RS18290 - hypothetical protein, at 35,078 to 35,251
_RS18290
LRK53_RS18295: LRK53_RS18295 - hypothetical protein, at 35,502 to 36,113
_RS18295
LRK53_RS18300: LRK53_RS18300 - hypothetical protein, at 36,200 to 37,108
_RS18300
LRK53_RS18305: LRK53_RS18305 - PD-(D/E)XK nuclease family protein, at 37,111 to 38,037
_RS18305
Position (kb) 36
37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 35.234 kb on - strand at 35.234 kb on - strand at 35.234 kb on - strand at 35.438 kb on + strand at 35.438 kb on + strand at 35.637 kb on + strand, within LRK53_RS18295 at 35.715 kb on + strand, within LRK53_RS18295 at 35.715 kb on + strand, within LRK53_RS18295 at 35.716 kb on - strand, within LRK53_RS18295 at 35.716 kb on - strand, within LRK53_RS18295 at 35.716 kb on - strand, within LRK53_RS18295 at 36.065 kb on + strand at 36.066 kb on - strand at 36.066 kb on - strand at 36.066 kb on - strand at 36.066 kb on - strand at 36.126 kb on + strand at 36.126 kb on + strand at 36.127 kb on - strand at 36.144 kb on + strand at 36.144 kb on + strand at 36.145 kb on - strand at 36.264 kb on - strand at 36.338 kb on + strand, within LRK53_RS18300 at 36.338 kb on + strand, within LRK53_RS18300 at 36.338 kb on + strand, within LRK53_RS18300 at 36.338 kb on + strand, within LRK53_RS18300 at 36.533 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.599 kb on + strand, within LRK53_RS18300 at 36.600 kb on - strand, within LRK53_RS18300 at 36.600 kb on - strand, within LRK53_RS18300 at 37.000 kb on + strand, within LRK53_RS18300 at 37.058 kb on + strand at 37.059 kb on - strand at 37.059 kb on - strand at 37.059 kb on - strand at 37.174 kb on + strand at 37.174 kb on + strand at 37.174 kb on + strand at 37.175 kb on - strand at 37.175 kb on - strand at 37.175 kb on - strand at 37.175 kb on - strand at 37.175 kb on - strand at 37.175 kb on - strand at 37.222 kb on + strand, within LRK53_RS18305 at 37.223 kb on - strand, within LRK53_RS18305 at 37.223 kb on - strand, within LRK53_RS18305 at 37.229 kb on - strand, within LRK53_RS18305 at 37.330 kb on + strand, within LRK53_RS18305 at 37.330 kb on + strand, within LRK53_RS18305 at 37.331 kb on - strand, within LRK53_RS18305 at 37.331 kb on - strand, within LRK53_RS18305 at 37.372 kb on + strand, within LRK53_RS18305 at 37.373 kb on - strand, within LRK53_RS18305 at 37.373 kb on - strand, within LRK53_RS18305 at 37.373 kb on - strand, within LRK53_RS18305 at 37.495 kb on + strand, within LRK53_RS18305 at 37.574 kb on - strand, within LRK53_RS18305 at 37.647 kb on + strand, within LRK53_RS18305 at 37.648 kb on - strand, within LRK53_RS18305 at 37.648 kb on - strand, within LRK53_RS18305 at 37.754 kb on + strand, within LRK53_RS18305 at 37.754 kb on + strand, within LRK53_RS18305 at 37.754 kb on + strand, within LRK53_RS18305 at 37.867 kb on + strand, within LRK53_RS18305 at 37.867 kb on + strand, within LRK53_RS18305 at 37.867 kb on + strand, within LRK53_RS18305 at 37.867 kb on + strand, within LRK53_RS18305 at 37.868 kb on - strand, within LRK53_RS18305 at 37.868 kb on - strand, within LRK53_RS18305 at 37.868 kb on - strand, within LRK53_RS18305 at 37.868 kb on - strand, within LRK53_RS18305 at 37.908 kb on + strand, within LRK53_RS18305 at 37.908 kb on + strand, within LRK53_RS18305 at 37.908 kb on + strand, within LRK53_RS18305 at 37.939 kb on + strand, within LRK53_RS18305 at 37.940 kb on - strand, within LRK53_RS18305 at 37.940 kb on - strand, within LRK53_RS18305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 35,234 - +1.0 35,234 - -1.5 35,234 - -1.1 35,438 + -1.5 35,438 + -2.0 35,637 + LRK53_RS18295 0.22 -0.4 35,715 + LRK53_RS18295 0.35 -1.1 35,715 + LRK53_RS18295 0.35 -2.0 35,716 - LRK53_RS18295 0.35 +0.6 35,716 - LRK53_RS18295 0.35 -1.3 35,716 - LRK53_RS18295 0.35 -0.3 36,065 + -1.5 36,066 - -1.2 36,066 - -0.8 36,066 - -0.8 36,066 - +0.4 36,126 + +1.0 36,126 + -0.1 36,127 - -0.8 36,144 + -0.6 36,144 + +0.3 36,145 - -0.0 36,264 - -0.4 36,338 + LRK53_RS18300 0.15 +0.8 36,338 + LRK53_RS18300 0.15 +0.8 36,338 + LRK53_RS18300 0.15 -1.3 36,338 + LRK53_RS18300 0.15 +0.5 36,533 + LRK53_RS18300 0.37 +0.4 36,599 + LRK53_RS18300 0.44 -0.9 36,599 + LRK53_RS18300 0.44 -1.1 36,599 + LRK53_RS18300 0.44 -1.8 36,600 - LRK53_RS18300 0.44 +0.7 36,600 - LRK53_RS18300 0.44 -0.4 37,000 + LRK53_RS18300 0.88 -0.9 37,058 + +1.6 37,059 - +2.0 37,059 - +2.0 37,059 - -1.2 37,174 + -1.9 37,174 + -0.3 37,174 + -0.8 37,175 - -0.0 37,175 - +1.3 37,175 - -0.6 37,175 - +0.4 37,175 - -0.3 37,175 - -0.6 37,222 + LRK53_RS18305 0.12 -0.8 37,223 - LRK53_RS18305 0.12 -0.7 37,223 - LRK53_RS18305 0.12 -2.9 37,229 - LRK53_RS18305 0.13 -0.1 37,330 + LRK53_RS18305 0.24 -1.6 37,330 + LRK53_RS18305 0.24 -2.0 37,331 - LRK53_RS18305 0.24 -0.0 37,331 - LRK53_RS18305 0.24 -0.0 37,372 + LRK53_RS18305 0.28 -0.7 37,373 - LRK53_RS18305 0.28 -1.5 37,373 - LRK53_RS18305 0.28 -1.3 37,373 - LRK53_RS18305 0.28 -0.3 37,495 + LRK53_RS18305 0.41 -0.7 37,574 - LRK53_RS18305 0.50 -1.7 37,647 + LRK53_RS18305 0.58 -1.1 37,648 - LRK53_RS18305 0.58 -1.0 37,648 - LRK53_RS18305 0.58 -1.2 37,754 + LRK53_RS18305 0.69 -0.6 37,754 + LRK53_RS18305 0.69 -0.4 37,754 + LRK53_RS18305 0.69 -2.3 37,867 + LRK53_RS18305 0.82 -2.2 37,867 + LRK53_RS18305 0.82 +0.7 37,867 + LRK53_RS18305 0.82 -2.0 37,867 + LRK53_RS18305 0.82 +0.3 37,868 - LRK53_RS18305 0.82 -0.0 37,868 - LRK53_RS18305 0.82 -1.7 37,868 - LRK53_RS18305 0.82 +0.6 37,868 - LRK53_RS18305 0.82 -1.1 37,908 + LRK53_RS18305 0.86 -0.7 37,908 + LRK53_RS18305 0.86 -0.0 37,908 + LRK53_RS18305 0.86 +1.0 37,939 + LRK53_RS18305 0.89 -1.3 37,940 - LRK53_RS18305 0.89 +1.0 37,940 - LRK53_RS18305 0.89 -1.0
Or see this region's nucleotide sequence