Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16590 and LRK53_RS16595 are separated by 319 nucleotides LRK53_RS16595 and LRK53_RS16600 are separated by 122 nucleotides LRK53_RS16600 and LRK53_RS16605 are separated by 91 nucleotides LRK53_RS16605 and LRK53_RS16610 are separated by 276 nucleotides
LRK53_RS16590: LRK53_RS16590 - ABC transporter permease, at 3,654,183 to 3,654,512
_RS16590
LRK53_RS16595: LRK53_RS16595 - hypothetical protein, at 3,654,832 to 3,654,990
_RS16595
LRK53_RS16600: LRK53_RS16600 - fumarylacetoacetate hydrolase family protein, at 3,655,113 to 3,655,799
_RS16600
LRK53_RS16605: LRK53_RS16605 - large-conductance mechanosensitive channel protein MscL, at 3,655,891 to 3,656,301
_RS16605
LRK53_RS16610: LRK53_RS16610 - IS4 family transposase, at 3,656,578 to 3,657,750
_RS16610
Position (kb)
3655
3656 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3654.184 kb on + strand at 3654.184 kb on + strand at 3654.361 kb on + strand, within LRK53_RS16590 at 3654.361 kb on + strand, within LRK53_RS16590 at 3654.372 kb on + strand, within LRK53_RS16590 at 3654.372 kb on + strand, within LRK53_RS16590 at 3654.373 kb on - strand, within LRK53_RS16590 at 3654.515 kb on + strand at 3654.516 kb on - strand at 3655.100 kb on + strand at 3655.101 kb on - strand at 3655.101 kb on - strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.119 kb on + strand at 3655.120 kb on - strand at 3655.120 kb on - strand at 3655.120 kb on - strand at 3655.120 kb on - strand at 3655.120 kb on - strand at 3655.120 kb on - strand at 3655.120 kb on - strand at 3655.120 kb on - strand at 3655.212 kb on + strand, within LRK53_RS16600 at 3655.212 kb on + strand, within LRK53_RS16600 at 3655.213 kb on - strand, within LRK53_RS16600 at 3655.317 kb on + strand, within LRK53_RS16600 at 3655.831 kb on + strand at 3655.831 kb on + strand at 3655.844 kb on + strand at 3655.845 kb on - strand at 3655.845 kb on - strand at 3655.881 kb on + strand at 3655.881 kb on + strand at 3655.881 kb on + strand at 3655.881 kb on + strand at 3655.881 kb on + strand at 3655.881 kb on + strand at 3655.881 kb on + strand at 3655.882 kb on - strand at 3655.882 kb on - strand at 3655.882 kb on - strand at 3655.882 kb on - strand at 3655.882 kb on - strand at 3656.128 kb on + strand, within LRK53_RS16605 at 3656.258 kb on + strand, within LRK53_RS16605 at 3656.258 kb on + strand, within LRK53_RS16605 at 3656.258 kb on + strand, within LRK53_RS16605 at 3656.258 kb on + strand, within LRK53_RS16605 at 3656.258 kb on + strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.259 kb on - strand, within LRK53_RS16605 at 3656.299 kb on + strand at 3656.299 kb on + strand at 3656.299 kb on + strand at 3656.299 kb on + strand at 3656.299 kb on + strand at 3656.299 kb on + strand at 3656.299 kb on + strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.300 kb on - strand at 3656.423 kb on - strand at 3656.423 kb on - strand at 3656.423 kb on - strand at 3656.423 kb on - strand at 3656.423 kb on - strand at 3656.423 kb on - strand at 3656.423 kb on - strand at 3656.446 kb on - strand at 3656.446 kb on - strand at 3656.446 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 3,654,184 + -0.2 3,654,184 + +0.8 3,654,361 + LRK53_RS16590 0.54 +0.4 3,654,361 + LRK53_RS16590 0.54 -0.9 3,654,372 + LRK53_RS16590 0.57 -0.0 3,654,372 + LRK53_RS16590 0.57 +0.6 3,654,373 - LRK53_RS16590 0.58 +1.4 3,654,515 + -1.0 3,654,516 - -0.7 3,655,100 + -1.0 3,655,101 - -0.0 3,655,101 - -0.9 3,655,119 + -0.2 3,655,119 + -0.2 3,655,119 + -0.2 3,655,119 + -0.6 3,655,119 + -1.1 3,655,119 + +0.4 3,655,119 + -0.2 3,655,119 + -0.2 3,655,119 + +1.4 3,655,119 + -0.6 3,655,120 - +0.4 3,655,120 - -1.3 3,655,120 - +0.4 3,655,120 - -1.3 3,655,120 - +1.0 3,655,120 - -1.0 3,655,120 - +0.4 3,655,120 - +0.4 3,655,212 + LRK53_RS16600 0.14 +0.0 3,655,212 + LRK53_RS16600 0.14 -1.2 3,655,213 - LRK53_RS16600 0.15 -1.4 3,655,317 + LRK53_RS16600 0.30 -1.7 3,655,831 + +1.4 3,655,831 + +0.4 3,655,844 + -0.1 3,655,845 - -1.0 3,655,845 - +1.0 3,655,881 + -0.2 3,655,881 + -0.7 3,655,881 + -0.8 3,655,881 + -2.5 3,655,881 + -2.0 3,655,881 + -0.0 3,655,881 + +0.1 3,655,882 - +0.4 3,655,882 - +1.4 3,655,882 - -0.4 3,655,882 - -0.7 3,655,882 - +1.4 3,656,128 + LRK53_RS16605 0.58 -0.6 3,656,258 + LRK53_RS16605 0.89 -1.2 3,656,258 + LRK53_RS16605 0.89 -0.4 3,656,258 + LRK53_RS16605 0.89 -1.4 3,656,258 + LRK53_RS16605 0.89 -0.2 3,656,258 + LRK53_RS16605 0.89 +0.8 3,656,259 - LRK53_RS16605 0.90 -0.6 3,656,259 - LRK53_RS16605 0.90 +0.4 3,656,259 - LRK53_RS16605 0.90 -2.5 3,656,259 - LRK53_RS16605 0.90 -0.4 3,656,259 - LRK53_RS16605 0.90 -1.2 3,656,259 - LRK53_RS16605 0.90 -1.4 3,656,259 - LRK53_RS16605 0.90 -0.2 3,656,259 - LRK53_RS16605 0.90 +0.2 3,656,259 - LRK53_RS16605 0.90 -1.2 3,656,259 - LRK53_RS16605 0.90 +1.4 3,656,299 + +1.2 3,656,299 + +1.4 3,656,299 + -1.2 3,656,299 + +0.4 3,656,299 + -1.6 3,656,299 + -0.3 3,656,299 + -0.3 3,656,300 - -0.2 3,656,300 - +0.1 3,656,300 - -1.1 3,656,300 - -0.2 3,656,300 - +0.8 3,656,300 - -1.0 3,656,300 - -2.3 3,656,300 - +1.0 3,656,300 - -0.2 3,656,300 - -1.4 3,656,300 - -1.3 3,656,300 - -2.2 3,656,423 - +0.4 3,656,423 - -0.8 3,656,423 - -1.1 3,656,423 - +0.7 3,656,423 - +3.0 3,656,423 - -1.2 3,656,423 - -0.9 3,656,446 - -1.0 3,656,446 - -1.6 3,656,446 - +2.4
Or see this region's nucleotide sequence