Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS15865 and LRK53_RS15870 are separated by 85 nucleotides LRK53_RS15870 and LRK53_RS15875 are separated by 141 nucleotides LRK53_RS15875 and LRK53_RS15880 are separated by 230 nucleotides
LRK53_RS15865: LRK53_RS15865 - hypothetical protein, at 3,468,848 to 3,470,554
_RS15865
LRK53_RS15870: LRK53_RS15870 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 3,470,640 to 3,472,775
_RS15870
LRK53_RS15875: LRK53_RS15875 - hypothetical protein, at 3,472,917 to 3,473,396
_RS15875
LRK53_RS15880: LRK53_RS15880 - IS5 family transposase, at 3,473,627 to 3,474,757
_RS15880
Position (kb)
3470
3471
3472
3473 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3469.890 kb on + strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.588 kb on - strand at 3470.588 kb on - strand at 3470.588 kb on - strand at 3470.588 kb on - strand at 3470.801 kb on + strand at 3470.802 kb on - strand at 3470.881 kb on + strand, within LRK53_RS15870 at 3470.881 kb on + strand, within LRK53_RS15870 at 3470.882 kb on - strand, within LRK53_RS15870 at 3470.882 kb on - strand, within LRK53_RS15870 at 3470.882 kb on - strand, within LRK53_RS15870 at 3470.882 kb on - strand, within LRK53_RS15870 at 3471.128 kb on + strand, within LRK53_RS15870 at 3471.410 kb on - strand, within LRK53_RS15870 at 3471.635 kb on - strand, within LRK53_RS15870 at 3471.770 kb on - strand, within LRK53_RS15870 at 3471.884 kb on - strand, within LRK53_RS15870 at 3471.910 kb on + strand, within LRK53_RS15870 at 3471.910 kb on + strand, within LRK53_RS15870 at 3471.910 kb on + strand, within LRK53_RS15870 at 3471.911 kb on - strand, within LRK53_RS15870 at 3471.911 kb on - strand, within LRK53_RS15870 at 3471.911 kb on - strand, within LRK53_RS15870 at 3471.911 kb on - strand, within LRK53_RS15870 at 3471.911 kb on - strand, within LRK53_RS15870 at 3471.911 kb on - strand, within LRK53_RS15870 at 3471.970 kb on + strand, within LRK53_RS15870 at 3471.971 kb on - strand, within LRK53_RS15870 at 3472.018 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.069 kb on + strand, within LRK53_RS15870 at 3472.070 kb on - strand, within LRK53_RS15870 at 3472.070 kb on - strand, within LRK53_RS15870 at 3472.792 kb on + strand at 3472.808 kb on + strand at 3473.154 kb on + strand, within LRK53_RS15875 at 3473.155 kb on - strand, within LRK53_RS15875 at 3473.155 kb on - strand, within LRK53_RS15875 at 3473.349 kb on + strand at 3473.349 kb on + strand at 3473.349 kb on + strand at 3473.349 kb on + strand at 3473.349 kb on + strand at 3473.349 kb on + strand at 3473.350 kb on - strand at 3473.350 kb on - strand at 3473.350 kb on - strand at 3473.350 kb on - strand at 3473.350 kb on - strand at 3473.350 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 3,469,890 + LRK53_RS15865 0.61 +0.3 3,469,891 - LRK53_RS15865 0.61 -0.0 3,469,891 - LRK53_RS15865 0.61 -1.7 3,469,891 - LRK53_RS15865 0.61 -0.6 3,469,891 - LRK53_RS15865 0.61 -0.7 3,470,587 + -0.0 3,470,587 + -0.7 3,470,587 + +1.3 3,470,587 + +0.9 3,470,587 + -1.9 3,470,587 + +0.3 3,470,587 + +0.0 3,470,588 - -1.3 3,470,588 - -0.0 3,470,588 - -0.5 3,470,588 - -2.0 3,470,801 + +0.3 3,470,802 - -0.3 3,470,881 + LRK53_RS15870 0.11 -0.7 3,470,881 + LRK53_RS15870 0.11 -1.5 3,470,882 - LRK53_RS15870 0.11 -0.3 3,470,882 - LRK53_RS15870 0.11 +1.3 3,470,882 - LRK53_RS15870 0.11 -0.3 3,470,882 - LRK53_RS15870 0.11 +0.3 3,471,128 + LRK53_RS15870 0.23 -1.3 3,471,410 - LRK53_RS15870 0.36 -1.3 3,471,635 - LRK53_RS15870 0.47 +1.3 3,471,770 - LRK53_RS15870 0.53 +0.7 3,471,884 - LRK53_RS15870 0.58 +0.3 3,471,910 + LRK53_RS15870 0.59 -1.4 3,471,910 + LRK53_RS15870 0.59 +0.9 3,471,910 + LRK53_RS15870 0.59 -1.0 3,471,911 - LRK53_RS15870 0.60 -1.7 3,471,911 - LRK53_RS15870 0.60 -0.9 3,471,911 - LRK53_RS15870 0.60 +2.3 3,471,911 - LRK53_RS15870 0.60 -0.1 3,471,911 - LRK53_RS15870 0.60 -1.1 3,471,911 - LRK53_RS15870 0.60 +0.3 3,471,970 + LRK53_RS15870 0.62 -1.3 3,471,971 - LRK53_RS15870 0.62 -1.7 3,472,018 + LRK53_RS15870 0.65 +1.3 3,472,069 + LRK53_RS15870 0.67 -0.7 3,472,069 + LRK53_RS15870 0.67 -1.0 3,472,069 + LRK53_RS15870 0.67 +1.3 3,472,069 + LRK53_RS15870 0.67 -0.3 3,472,069 + LRK53_RS15870 0.67 +1.3 3,472,069 + LRK53_RS15870 0.67 -0.3 3,472,069 + LRK53_RS15870 0.67 -1.3 3,472,069 + LRK53_RS15870 0.67 +1.3 3,472,069 + LRK53_RS15870 0.67 +1.3 3,472,069 + LRK53_RS15870 0.67 +0.3 3,472,069 + LRK53_RS15870 0.67 -0.3 3,472,070 - LRK53_RS15870 0.67 +1.3 3,472,070 - LRK53_RS15870 0.67 -1.5 3,472,792 + +1.3 3,472,808 + -1.9 3,473,154 + LRK53_RS15875 0.49 -1.0 3,473,155 - LRK53_RS15875 0.50 -0.1 3,473,155 - LRK53_RS15875 0.50 +0.3 3,473,349 + -1.3 3,473,349 + +0.3 3,473,349 + +0.7 3,473,349 + -1.3 3,473,349 + +1.3 3,473,349 + +0.3 3,473,350 - +2.9 3,473,350 - +1.3 3,473,350 - -1.2 3,473,350 - -2.2 3,473,350 - +0.3 3,473,350 - -1.0
Or see this region's nucleotide sequence