Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS14940 and LRK53_RS14945 are separated by 13 nucleotides LRK53_RS14945 and LRK53_RS14950 are separated by 234 nucleotides LRK53_RS14950 and LRK53_RS14955 overlap by 4 nucleotides LRK53_RS14955 and LRK53_RS14960 are separated by 16 nucleotides LRK53_RS14960 and LRK53_RS14965 overlap by 11 nucleotides
LRK53_RS14940: LRK53_RS14940 - response regulator, at 3,269,299 to 3,269,661
_RS14940
LRK53_RS14945: LRK53_RS14945 - STAS domain-containing protein, at 3,269,675 to 3,270,070
_RS14945
LRK53_RS14950: LRK53_RS14950 - flagellar hook-length control protein FliK, at 3,270,305 to 3,271,447
_RS14950
LRK53_RS14955: LRK53_RS14955 - hypothetical protein, at 3,271,444 to 3,271,704
_RS14955
LRK53_RS14960: LRK53_RS14960 - DUF2802 domain-containing protein, at 3,271,721 to 3,272,068
_RS14960
LRK53_RS14965: LRK53_RS14965 - chemotaxis protein CheW, at 3,272,058 to 3,272,537
_RS14965
Position (kb)
3270
3271
3272 Strain fitness (log2 ratio)
-1
0
1
2 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.444 kb on + strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3269.445 kb on - strand, within LRK53_RS14940 at 3271.275 kb on + strand, within LRK53_RS14950 at 3271.275 kb on + strand, within LRK53_RS14950 at 3271.362 kb on + strand at 3271.363 kb on - strand at 3271.459 kb on + strand at 3271.460 kb on - strand at 3271.652 kb on - strand, within LRK53_RS14955 at 3271.684 kb on + strand at 3271.684 kb on + strand at 3271.685 kb on - strand at 3271.685 kb on - strand at 3271.839 kb on + strand, within LRK53_RS14960 at 3271.839 kb on + strand, within LRK53_RS14960 at 3271.840 kb on - strand, within LRK53_RS14960 at 3272.376 kb on + strand, within LRK53_RS14965 at 3272.377 kb on - strand, within LRK53_RS14965
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 3,269,444 + LRK53_RS14940 0.40 -1.1 3,269,444 + LRK53_RS14940 0.40 +1.3 3,269,444 + LRK53_RS14940 0.40 +0.7 3,269,444 + LRK53_RS14940 0.40 -1.1 3,269,444 + LRK53_RS14940 0.40 -1.2 3,269,444 + LRK53_RS14940 0.40 +0.5 3,269,444 + LRK53_RS14940 0.40 +2.6 3,269,444 + LRK53_RS14940 0.40 +0.3 3,269,444 + LRK53_RS14940 0.40 -0.3 3,269,444 + LRK53_RS14940 0.40 -0.5 3,269,444 + LRK53_RS14940 0.40 -0.3 3,269,444 + LRK53_RS14940 0.40 -0.3 3,269,445 - LRK53_RS14940 0.40 -1.7 3,269,445 - LRK53_RS14940 0.40 -0.1 3,269,445 - LRK53_RS14940 0.40 +0.3 3,269,445 - LRK53_RS14940 0.40 -1.2 3,269,445 - LRK53_RS14940 0.40 -0.3 3,269,445 - LRK53_RS14940 0.40 +0.0 3,269,445 - LRK53_RS14940 0.40 +1.3 3,269,445 - LRK53_RS14940 0.40 +0.3 3,269,445 - LRK53_RS14940 0.40 -1.3 3,269,445 - LRK53_RS14940 0.40 -1.1 3,271,275 + LRK53_RS14950 0.85 -0.7 3,271,275 + LRK53_RS14950 0.85 -1.7 3,271,362 + -0.3 3,271,363 - +1.0 3,271,459 + -0.5 3,271,460 - +1.3 3,271,652 - LRK53_RS14955 0.80 +1.3 3,271,684 + -0.7 3,271,684 + -0.1 3,271,685 - +1.3 3,271,685 - +0.7 3,271,839 + LRK53_RS14960 0.34 +0.3 3,271,839 + LRK53_RS14960 0.34 +0.3 3,271,840 - LRK53_RS14960 0.34 -0.4 3,272,376 + LRK53_RS14965 0.66 +0.7 3,272,377 - LRK53_RS14965 0.66 +0.3
Or see this region's nucleotide sequence