Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS13555 and LRK53_RS13560 are separated by 56 nucleotides LRK53_RS13560 and LRK53_RS13565 are separated by 174 nucleotides LRK53_RS13565 and LRK53_RS13570 are separated by 238 nucleotides LRK53_RS13570 and LRK53_RS13575 overlap by 4 nucleotides
LRK53_RS13555: LRK53_RS13555 - aspartyl/asparaginyl beta-hydroxylase domain-containing protein, at 2,989,873 to 2,990,826
_RS13555
LRK53_RS13560: LRK53_RS13560 - RNA polymerase subunit sigma-24, at 2,990,883 to 2,991,071
_RS13560
LRK53_RS13565: LRK53_RS13565 - isocitrate dehydrogenase, at 2,991,246 to 2,992,259
_RS13565
LRK53_RS13570: LRK53_RS13570 - carboxymuconolactone decarboxylase family protein, at 2,992,498 to 2,992,875
_RS13570
LRK53_RS13575: LRK53_RS13575 - glutaredoxin 3, at 2,992,872 to 2,993,135
_RS13575
Position (kb)
2991
2992
2993 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2990.386 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.491 kb on + strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.492 kb on - strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.677 kb on + strand, within LRK53_RS13555 at 2990.678 kb on - strand, within LRK53_RS13555 at 2990.678 kb on - strand, within LRK53_RS13555 at 2990.678 kb on - strand, within LRK53_RS13555 at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.749 kb on + strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.750 kb on - strand at 2990.764 kb on + strand at 2990.764 kb on + strand at 2990.764 kb on + strand at 2990.764 kb on + strand at 2990.764 kb on + strand at 2990.764 kb on + strand at 2990.765 kb on - strand at 2990.765 kb on - strand at 2990.765 kb on - strand at 2990.765 kb on - strand at 2990.765 kb on - strand at 2990.765 kb on - strand at 2990.765 kb on - strand at 2990.765 kb on - strand at 2991.139 kb on + strand at 2991.139 kb on + strand at 2991.139 kb on + strand at 2991.139 kb on + strand at 2991.139 kb on + strand at 2991.139 kb on + strand at 2991.139 kb on + strand at 2991.140 kb on - strand at 2991.140 kb on - strand at 2991.140 kb on - strand at 2991.140 kb on - strand at 2991.140 kb on - strand at 2991.140 kb on - strand at 2991.140 kb on - strand at 2991.140 kb on - strand at 2991.142 kb on - strand at 2991.169 kb on - strand at 2991.169 kb on - strand at 2991.169 kb on - strand at 2991.611 kb on - strand, within LRK53_RS13565 at 2991.676 kb on + strand, within LRK53_RS13565 at 2991.889 kb on + strand, within LRK53_RS13565 at 2991.890 kb on - strand, within LRK53_RS13565 at 2991.938 kb on - strand, within LRK53_RS13565 at 2992.160 kb on - strand at 2992.160 kb on - strand at 2992.426 kb on - strand at 2992.426 kb on - strand at 2992.499 kb on + strand at 2992.499 kb on + strand at 2992.499 kb on + strand at 2992.499 kb on + strand at 2992.499 kb on + strand at 2992.499 kb on + strand at 2992.499 kb on + strand at 2992.500 kb on - strand at 2992.500 kb on - strand at 2992.500 kb on - strand at 2992.500 kb on - strand at 2992.500 kb on - strand at 2992.500 kb on - strand at 2992.500 kb on - strand at 2992.500 kb on - strand at 2992.676 kb on + strand, within LRK53_RS13570 at 2992.676 kb on + strand, within LRK53_RS13570 at 2992.676 kb on + strand, within LRK53_RS13570 at 2992.676 kb on + strand, within LRK53_RS13570 at 2992.676 kb on + strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.677 kb on - strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.763 kb on + strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.764 kb on - strand, within LRK53_RS13570 at 2992.774 kb on - strand, within LRK53_RS13570 at 2992.774 kb on - strand, within LRK53_RS13570 at 2992.936 kb on + strand, within LRK53_RS13575 at 2993.089 kb on - strand, within LRK53_RS13575 at 2993.089 kb on - strand, within LRK53_RS13575 at 2993.095 kb on + strand, within LRK53_RS13575 at 2993.096 kb on - strand, within LRK53_RS13575 at 2993.096 kb on - strand, within LRK53_RS13575 at 2993.103 kb on + strand, within LRK53_RS13575 at 2993.103 kb on + strand, within LRK53_RS13575 at 2993.104 kb on - strand, within LRK53_RS13575 at 2993.116 kb on + strand at 2993.116 kb on + strand at 2993.116 kb on + strand at 2993.116 kb on + strand at 2993.116 kb on + strand at 2993.116 kb on + strand at 2993.117 kb on - strand at 2993.117 kb on - strand at 2993.117 kb on - strand at 2993.117 kb on - strand at 2993.117 kb on - strand at 2993.222 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.246 kb on + strand at 2993.247 kb on - strand at 2993.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 2,990,386 + LRK53_RS13555 0.54 -1.3 2,990,491 + LRK53_RS13555 0.65 -1.3 2,990,491 + LRK53_RS13555 0.65 +1.3 2,990,491 + LRK53_RS13555 0.65 -1.5 2,990,491 + LRK53_RS13555 0.65 +1.3 2,990,491 + LRK53_RS13555 0.65 -0.3 2,990,491 + LRK53_RS13555 0.65 +1.9 2,990,491 + LRK53_RS13555 0.65 -0.5 2,990,491 + LRK53_RS13555 0.65 +0.6 2,990,491 + LRK53_RS13555 0.65 -0.2 2,990,491 + LRK53_RS13555 0.65 +0.2 2,990,491 + LRK53_RS13555 0.65 -0.6 2,990,491 + LRK53_RS13555 0.65 -0.3 2,990,491 + LRK53_RS13555 0.65 -1.0 2,990,491 + LRK53_RS13555 0.65 +0.3 2,990,491 + LRK53_RS13555 0.65 -1.0 2,990,491 + LRK53_RS13555 0.65 -0.6 2,990,491 + LRK53_RS13555 0.65 -0.3 2,990,491 + LRK53_RS13555 0.65 -0.6 2,990,491 + LRK53_RS13555 0.65 -0.9 2,990,491 + LRK53_RS13555 0.65 -0.0 2,990,491 + LRK53_RS13555 0.65 -0.0 2,990,491 + LRK53_RS13555 0.65 +0.6 2,990,491 + LRK53_RS13555 0.65 +0.8 2,990,491 + LRK53_RS13555 0.65 +0.3 2,990,491 + LRK53_RS13555 0.65 +0.7 2,990,491 + LRK53_RS13555 0.65 +0.3 2,990,492 - LRK53_RS13555 0.65 +0.3 2,990,492 - LRK53_RS13555 0.65 -1.7 2,990,492 - LRK53_RS13555 0.65 +0.5 2,990,492 - LRK53_RS13555 0.65 +0.3 2,990,492 - LRK53_RS13555 0.65 -0.9 2,990,492 - LRK53_RS13555 0.65 +0.5 2,990,492 - LRK53_RS13555 0.65 -2.0 2,990,492 - LRK53_RS13555 0.65 +0.9 2,990,492 - LRK53_RS13555 0.65 -1.0 2,990,492 - LRK53_RS13555 0.65 +0.3 2,990,492 - LRK53_RS13555 0.65 -0.6 2,990,492 - LRK53_RS13555 0.65 +0.9 2,990,492 - LRK53_RS13555 0.65 -0.3 2,990,492 - LRK53_RS13555 0.65 +0.2 2,990,492 - LRK53_RS13555 0.65 -0.3 2,990,492 - LRK53_RS13555 0.65 -0.3 2,990,492 - LRK53_RS13555 0.65 +0.6 2,990,492 - LRK53_RS13555 0.65 -0.9 2,990,492 - LRK53_RS13555 0.65 -1.3 2,990,492 - LRK53_RS13555 0.65 -1.3 2,990,492 - LRK53_RS13555 0.65 +0.3 2,990,492 - LRK53_RS13555 0.65 +2.3 2,990,492 - LRK53_RS13555 0.65 +1.3 2,990,492 - LRK53_RS13555 0.65 -0.0 2,990,492 - LRK53_RS13555 0.65 -0.9 2,990,677 + LRK53_RS13555 0.84 -0.5 2,990,677 + LRK53_RS13555 0.84 -0.1 2,990,677 + LRK53_RS13555 0.84 +1.3 2,990,677 + LRK53_RS13555 0.84 -1.3 2,990,677 + LRK53_RS13555 0.84 -1.2 2,990,677 + LRK53_RS13555 0.84 -0.7 2,990,677 + LRK53_RS13555 0.84 -1.9 2,990,677 + LRK53_RS13555 0.84 -1.2 2,990,678 - LRK53_RS13555 0.84 +1.3 2,990,678 - LRK53_RS13555 0.84 +0.3 2,990,678 - LRK53_RS13555 0.84 +1.0 2,990,749 + +0.4 2,990,749 + -0.3 2,990,749 + +1.3 2,990,749 + +1.3 2,990,749 + +0.1 2,990,749 + +1.1 2,990,749 + +0.3 2,990,749 + -0.9 2,990,749 + -0.0 2,990,749 + -1.1 2,990,749 + +1.1 2,990,749 + -1.0 2,990,749 + -0.7 2,990,749 + -0.1 2,990,749 + -0.3 2,990,749 + +1.0 2,990,749 + -0.0 2,990,749 + +1.0 2,990,749 + -1.0 2,990,749 + +1.3 2,990,750 - +0.1 2,990,750 - +1.3 2,990,750 - +1.3 2,990,750 - -0.7 2,990,750 - +0.1 2,990,750 - +1.3 2,990,750 - -0.7 2,990,750 - -0.4 2,990,750 - +0.3 2,990,750 - +0.6 2,990,750 - -0.4 2,990,750 - -0.3 2,990,750 - -0.3 2,990,750 - -1.0 2,990,750 - +0.3 2,990,750 - +0.7 2,990,750 - -0.3 2,990,750 - +1.0 2,990,750 - -0.8 2,990,750 - +0.8 2,990,750 - -1.0 2,990,750 - +0.3 2,990,750 - +2.6 2,990,750 - +1.3 2,990,764 + -0.5 2,990,764 + +1.5 2,990,764 + +0.6 2,990,764 + -0.4 2,990,764 + +0.3 2,990,764 + +1.3 2,990,765 - -1.0 2,990,765 - -1.3 2,990,765 - +0.7 2,990,765 - -1.0 2,990,765 - -0.7 2,990,765 - -0.4 2,990,765 - -0.6 2,990,765 - -0.7 2,991,139 + -1.2 2,991,139 + +0.1 2,991,139 + -0.9 2,991,139 + -0.7 2,991,139 + -0.2 2,991,139 + -1.5 2,991,139 + -0.7 2,991,140 - +0.3 2,991,140 - +0.4 2,991,140 - -0.9 2,991,140 - -1.0 2,991,140 - -0.5 2,991,140 - +0.7 2,991,140 - -0.3 2,991,140 - -0.1 2,991,142 - -0.8 2,991,169 - -1.5 2,991,169 - +2.9 2,991,169 - +0.8 2,991,611 - LRK53_RS13565 0.36 +0.7 2,991,676 + LRK53_RS13565 0.42 -1.5 2,991,889 + LRK53_RS13565 0.63 -1.0 2,991,890 - LRK53_RS13565 0.64 +1.3 2,991,938 - LRK53_RS13565 0.68 -0.3 2,992,160 - +1.3 2,992,160 - -1.0 2,992,426 - +1.0 2,992,426 - -0.3 2,992,499 + +0.3 2,992,499 + -0.3 2,992,499 + -1.4 2,992,499 + +0.5 2,992,499 + -1.5 2,992,499 + -0.3 2,992,499 + +1.3 2,992,500 - -0.0 2,992,500 - -1.7 2,992,500 - +2.3 2,992,500 - -0.6 2,992,500 - -1.0 2,992,500 - -0.7 2,992,500 - -0.7 2,992,500 - -0.6 2,992,676 + LRK53_RS13570 0.47 -1.0 2,992,676 + LRK53_RS13570 0.47 -0.1 2,992,676 + LRK53_RS13570 0.47 -0.0 2,992,676 + LRK53_RS13570 0.47 +1.3 2,992,676 + LRK53_RS13570 0.47 +0.3 2,992,677 - LRK53_RS13570 0.47 -1.6 2,992,677 - LRK53_RS13570 0.47 -1.0 2,992,677 - LRK53_RS13570 0.47 -1.0 2,992,677 - LRK53_RS13570 0.47 +2.0 2,992,677 - LRK53_RS13570 0.47 +1.3 2,992,677 - LRK53_RS13570 0.47 -1.0 2,992,677 - LRK53_RS13570 0.47 +1.3 2,992,677 - LRK53_RS13570 0.47 +0.1 2,992,763 + LRK53_RS13570 0.70 -1.3 2,992,763 + LRK53_RS13570 0.70 +0.3 2,992,763 + LRK53_RS13570 0.70 +0.3 2,992,763 + LRK53_RS13570 0.70 +0.7 2,992,763 + LRK53_RS13570 0.70 -1.2 2,992,763 + LRK53_RS13570 0.70 -0.3 2,992,763 + LRK53_RS13570 0.70 -1.0 2,992,763 + LRK53_RS13570 0.70 +1.5 2,992,763 + LRK53_RS13570 0.70 -0.1 2,992,763 + LRK53_RS13570 0.70 +0.7 2,992,763 + LRK53_RS13570 0.70 -0.0 2,992,763 + LRK53_RS13570 0.70 -0.4 2,992,763 + LRK53_RS13570 0.70 +0.3 2,992,764 - LRK53_RS13570 0.70 -0.5 2,992,764 - LRK53_RS13570 0.70 +1.3 2,992,764 - LRK53_RS13570 0.70 -1.0 2,992,764 - LRK53_RS13570 0.70 -0.0 2,992,764 - LRK53_RS13570 0.70 +0.9 2,992,764 - LRK53_RS13570 0.70 +0.3 2,992,764 - LRK53_RS13570 0.70 -1.0 2,992,764 - LRK53_RS13570 0.70 +0.3 2,992,764 - LRK53_RS13570 0.70 +2.3 2,992,764 - LRK53_RS13570 0.70 -1.3 2,992,764 - LRK53_RS13570 0.70 +0.5 2,992,764 - LRK53_RS13570 0.70 +0.3 2,992,764 - LRK53_RS13570 0.70 -1.3 2,992,774 - LRK53_RS13570 0.73 -1.3 2,992,774 - LRK53_RS13570 0.73 -0.7 2,992,936 + LRK53_RS13575 0.24 -1.0 2,993,089 - LRK53_RS13575 0.82 +0.4 2,993,089 - LRK53_RS13575 0.82 +0.3 2,993,095 + LRK53_RS13575 0.84 -0.7 2,993,096 - LRK53_RS13575 0.85 -1.2 2,993,096 - LRK53_RS13575 0.85 -0.3 2,993,103 + LRK53_RS13575 0.88 -0.5 2,993,103 + LRK53_RS13575 0.88 -0.3 2,993,104 - LRK53_RS13575 0.88 -0.5 2,993,116 + +2.3 2,993,116 + -0.0 2,993,116 + -1.0 2,993,116 + -1.0 2,993,116 + -0.7 2,993,116 + +0.3 2,993,117 - +0.3 2,993,117 - +1.1 2,993,117 - +0.3 2,993,117 - -0.7 2,993,117 - -0.3 2,993,222 + +0.7 2,993,246 + +0.4 2,993,246 + -0.7 2,993,246 + -0.4 2,993,246 + +0.2 2,993,246 + -0.4 2,993,246 + -0.3 2,993,246 + +0.3 2,993,246 + +0.3 2,993,246 + -0.7 2,993,247 - -2.5 2,993,247 - +1.3
Or see this region's nucleotide sequence