Strain Fitness in Rhodanobacter sp000427505 FW510-R12 around LRK53_RS12740

Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLRK53_RS12730 and LRK53_RS12735 overlap by 4 nucleotidesLRK53_RS12735 and LRK53_RS12740 are separated by 71 nucleotidesLRK53_RS12740 and LRK53_RS12745 overlap by 4 nucleotides LRK53_RS12730: LRK53_RS12730 - heme lyase CcmF/NrfE family subunit, at 2,814,363 to 2,816,342 _RS12730 LRK53_RS12735: LRK53_RS12735 - DsbE family thiol:disulfide interchange protein, at 2,816,339 to 2,816,881 _RS12735 LRK53_RS12740: LRK53_RS12740 - cytochrome c-type biogenesis protein CcmH, at 2,816,953 to 2,817,399 _RS12740 LRK53_RS12745: LRK53_RS12745 - cytochrome C biogenesis protein CcmI, at 2,817,396 to 2,818,448 _RS12745 Position (kb) 2816 2817 2818Strain fitness (log2 ratio) -2 -1 0 1 2at 2816.948 kb on + strandat 2816.948 kb on + strandat 2816.948 kb on + strandat 2816.948 kb on + strandat 2816.948 kb on + strandat 2816.948 kb on + strandat 2816.948 kb on + strandat 2816.948 kb on + strandat 2816.948 kb on + strandat 2817.302 kb on + strand, within LRK53_RS12740at 2817.302 kb on + strand, within LRK53_RS12740at 2817.302 kb on + strand, within LRK53_RS12740at 2817.302 kb on + strand, within LRK53_RS12740at 2817.302 kb on + strand, within LRK53_RS12740at 2817.561 kb on + strand, within LRK53_RS12745

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
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2,816,948 + +1.3
2,816,948 + +2.3
2,816,948 + -0.3
2,816,948 + -1.5
2,816,948 + -0.7
2,816,948 + +0.3
2,816,948 + -1.1
2,816,948 + +1.3
2,816,948 + -1.7
2,817,302 + LRK53_RS12740 0.78 -1.1
2,817,302 + LRK53_RS12740 0.78 -1.2
2,817,302 + LRK53_RS12740 0.78 -0.7
2,817,302 + LRK53_RS12740 0.78 -1.5
2,817,302 + LRK53_RS12740 0.78 -2.6
2,817,561 + LRK53_RS12745 0.16 +1.3

Or see this region's nucleotide sequence