Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS10950 and LRK53_RS10955 are separated by 34 nucleotides LRK53_RS10955 and LRK53_RS10960 are separated by 7 nucleotides
LRK53_RS10950: LRK53_RS10950 - DEAD/DEAH box helicase, at 2,445,891 to 2,446,979
_RS10950
LRK53_RS10955: LRK53_RS10955 - phosphopyruvate hydratase, at 2,447,014 to 2,448,294
_RS10955
LRK53_RS10960: LRK53_RS10960 - DNA mismatch repair protein MutS, at 2,448,302 to 2,449,843
_RS10960
Position (kb)
2447
2448
2449 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2446.098 kb on + strand, within LRK53_RS10950 at 2446.099 kb on - strand, within LRK53_RS10950 at 2446.099 kb on - strand, within LRK53_RS10950 at 2446.099 kb on - strand, within LRK53_RS10950 at 2446.208 kb on - strand, within LRK53_RS10950 at 2446.208 kb on - strand, within LRK53_RS10950 at 2446.299 kb on + strand, within LRK53_RS10950 at 2446.393 kb on + strand, within LRK53_RS10950 at 2446.397 kb on - strand, within LRK53_RS10950 at 2446.731 kb on + strand, within LRK53_RS10950 at 2446.816 kb on - strand, within LRK53_RS10950 at 2447.010 kb on - strand at 2447.010 kb on - strand at 2447.188 kb on + strand, within LRK53_RS10955 at 2447.216 kb on + strand, within LRK53_RS10955 at 2447.217 kb on - strand, within LRK53_RS10955 at 2447.217 kb on - strand, within LRK53_RS10955 at 2447.366 kb on - strand, within LRK53_RS10955 at 2447.444 kb on + strand, within LRK53_RS10955 at 2447.445 kb on - strand, within LRK53_RS10955 at 2447.506 kb on + strand, within LRK53_RS10955 at 2447.506 kb on + strand, within LRK53_RS10955 at 2447.506 kb on + strand, within LRK53_RS10955 at 2447.506 kb on + strand, within LRK53_RS10955 at 2447.506 kb on + strand, within LRK53_RS10955 at 2447.507 kb on - strand, within LRK53_RS10955 at 2447.507 kb on - strand, within LRK53_RS10955 at 2447.852 kb on - strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.100 kb on + strand, within LRK53_RS10955 at 2448.101 kb on - strand, within LRK53_RS10955 at 2448.101 kb on - strand, within LRK53_RS10955 at 2448.101 kb on - strand, within LRK53_RS10955 at 2448.101 kb on - strand, within LRK53_RS10955 at 2448.101 kb on - strand, within LRK53_RS10955 at 2448.101 kb on - strand, within LRK53_RS10955 at 2448.207 kb on + strand at 2448.207 kb on + strand at 2448.311 kb on - strand at 2448.514 kb on - strand, within LRK53_RS10960 at 2449.047 kb on + strand, within LRK53_RS10960 at 2449.047 kb on + strand, within LRK53_RS10960 at 2449.090 kb on + strand, within LRK53_RS10960 at 2449.091 kb on - strand, within LRK53_RS10960 at 2449.239 kb on + strand, within LRK53_RS10960 at 2449.239 kb on + strand, within LRK53_RS10960 at 2449.240 kb on - strand, within LRK53_RS10960 at 2449.240 kb on - strand, within LRK53_RS10960 at 2449.251 kb on + strand, within LRK53_RS10960 at 2449.251 kb on + strand, within LRK53_RS10960 at 2449.251 kb on + strand, within LRK53_RS10960 at 2449.252 kb on - strand, within LRK53_RS10960 at 2449.252 kb on - strand, within LRK53_RS10960 at 2449.272 kb on + strand, within LRK53_RS10960 at 2449.272 kb on + strand, within LRK53_RS10960 at 2449.272 kb on + strand, within LRK53_RS10960 at 2449.273 kb on - strand, within LRK53_RS10960 at 2449.273 kb on - strand, within LRK53_RS10960 at 2449.273 kb on - strand, within LRK53_RS10960 at 2449.273 kb on - strand, within LRK53_RS10960 at 2449.273 kb on - strand, within LRK53_RS10960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 2,446,098 + LRK53_RS10950 0.19 -0.3 2,446,099 - LRK53_RS10950 0.19 -0.3 2,446,099 - LRK53_RS10950 0.19 +1.2 2,446,099 - LRK53_RS10950 0.19 +0.8 2,446,208 - LRK53_RS10950 0.29 +1.2 2,446,208 - LRK53_RS10950 0.29 +0.9 2,446,299 + LRK53_RS10950 0.37 -0.4 2,446,393 + LRK53_RS10950 0.46 +1.2 2,446,397 - LRK53_RS10950 0.46 -0.5 2,446,731 + LRK53_RS10950 0.77 +0.6 2,446,816 - LRK53_RS10950 0.85 +1.2 2,447,010 - -0.4 2,447,010 - -0.4 2,447,188 + LRK53_RS10955 0.14 -0.4 2,447,216 + LRK53_RS10955 0.16 -0.8 2,447,217 - LRK53_RS10955 0.16 +0.6 2,447,217 - LRK53_RS10955 0.16 +1.2 2,447,366 - LRK53_RS10955 0.27 -1.1 2,447,444 + LRK53_RS10955 0.34 +1.2 2,447,445 - LRK53_RS10955 0.34 -0.6 2,447,506 + LRK53_RS10955 0.38 +0.2 2,447,506 + LRK53_RS10955 0.38 -2.4 2,447,506 + LRK53_RS10955 0.38 -2.1 2,447,506 + LRK53_RS10955 0.38 +1.2 2,447,506 + LRK53_RS10955 0.38 -2.0 2,447,507 - LRK53_RS10955 0.38 +0.9 2,447,507 - LRK53_RS10955 0.38 -1.1 2,447,852 - LRK53_RS10955 0.65 -1.4 2,448,100 + LRK53_RS10955 0.85 -1.6 2,448,100 + LRK53_RS10955 0.85 +0.5 2,448,100 + LRK53_RS10955 0.85 -1.1 2,448,100 + LRK53_RS10955 0.85 -0.5 2,448,100 + LRK53_RS10955 0.85 -1.5 2,448,100 + LRK53_RS10955 0.85 -0.8 2,448,100 + LRK53_RS10955 0.85 -0.4 2,448,100 + LRK53_RS10955 0.85 -0.2 2,448,100 + LRK53_RS10955 0.85 -1.4 2,448,100 + LRK53_RS10955 0.85 -0.8 2,448,101 - LRK53_RS10955 0.85 -0.4 2,448,101 - LRK53_RS10955 0.85 +1.2 2,448,101 - LRK53_RS10955 0.85 -0.4 2,448,101 - LRK53_RS10955 0.85 -1.1 2,448,101 - LRK53_RS10955 0.85 -1.1 2,448,101 - LRK53_RS10955 0.85 +3.2 2,448,207 + -0.6 2,448,207 + +1.2 2,448,311 - +0.9 2,448,514 - LRK53_RS10960 0.14 +0.2 2,449,047 + LRK53_RS10960 0.48 +0.4 2,449,047 + LRK53_RS10960 0.48 -0.4 2,449,090 + LRK53_RS10960 0.51 -0.8 2,449,091 - LRK53_RS10960 0.51 +0.2 2,449,239 + LRK53_RS10960 0.61 +1.2 2,449,239 + LRK53_RS10960 0.61 +0.2 2,449,240 - LRK53_RS10960 0.61 +0.5 2,449,240 - LRK53_RS10960 0.61 +0.2 2,449,251 + LRK53_RS10960 0.62 -0.1 2,449,251 + LRK53_RS10960 0.62 -0.2 2,449,251 + LRK53_RS10960 0.62 +0.2 2,449,252 - LRK53_RS10960 0.62 -2.1 2,449,252 - LRK53_RS10960 0.62 -0.8 2,449,272 + LRK53_RS10960 0.63 -0.1 2,449,272 + LRK53_RS10960 0.63 +0.1 2,449,272 + LRK53_RS10960 0.63 +0.2 2,449,273 - LRK53_RS10960 0.63 -0.6 2,449,273 - LRK53_RS10960 0.63 +1.2 2,449,273 - LRK53_RS10960 0.63 -1.6 2,449,273 - LRK53_RS10960 0.63 +0.2 2,449,273 - LRK53_RS10960 0.63 -0.1
Or see this region's nucleotide sequence