Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09715 and LRK53_RS09720 are separated by 5 nucleotides LRK53_RS09720 and LRK53_RS09725 are separated by 117 nucleotides LRK53_RS09725 and LRK53_RS09730 are separated by 66 nucleotides
LRK53_RS09715: LRK53_RS09715 - tRNA 2-thiouridine(34) synthase MnmA, at 2,186,283 to 2,187,422
_RS09715
LRK53_RS09720: LRK53_RS09720 - NUDIX hydrolase, at 2,187,428 to 2,187,913
_RS09720
LRK53_RS09725: LRK53_RS09725 - ATP-dependent Clp protease adapter ClpS, at 2,188,031 to 2,188,357
_RS09725
LRK53_RS09730: LRK53_RS09730 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 2,188,424 to 2,190,694
_RS09730
Position (kb)
2188
2189 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.997 kb on + strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.998 kb on - strand at 2187.999 kb on + strand at 2187.999 kb on + strand at 2187.999 kb on + strand at 2187.999 kb on + strand at 2187.999 kb on + strand at 2188.000 kb on - strand at 2188.125 kb on + strand, within LRK53_RS09725 at 2188.125 kb on + strand, within LRK53_RS09725 at 2188.125 kb on + strand, within LRK53_RS09725 at 2188.125 kb on + strand, within LRK53_RS09725 at 2188.125 kb on + strand, within LRK53_RS09725 at 2188.125 kb on + strand, within LRK53_RS09725 at 2188.125 kb on + strand, within LRK53_RS09725 at 2188.126 kb on - strand, within LRK53_RS09725 at 2188.126 kb on - strand, within LRK53_RS09725 at 2188.126 kb on - strand, within LRK53_RS09725 at 2188.180 kb on + strand, within LRK53_RS09725 at 2188.180 kb on + strand, within LRK53_RS09725 at 2188.180 kb on + strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.181 kb on - strand, within LRK53_RS09725 at 2188.234 kb on + strand, within LRK53_RS09725 at 2188.234 kb on + strand, within LRK53_RS09725 at 2188.234 kb on + strand, within LRK53_RS09725 at 2188.251 kb on + strand, within LRK53_RS09725 at 2188.251 kb on + strand, within LRK53_RS09725 at 2188.251 kb on + strand, within LRK53_RS09725 at 2188.251 kb on + strand, within LRK53_RS09725 at 2188.252 kb on - strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.260 kb on + strand, within LRK53_RS09725 at 2188.261 kb on - strand, within LRK53_RS09725 at 2188.261 kb on - strand, within LRK53_RS09725 at 2188.261 kb on - strand, within LRK53_RS09725 at 2188.261 kb on - strand, within LRK53_RS09725 at 2188.261 kb on - strand, within LRK53_RS09725 at 2188.261 kb on - strand, within LRK53_RS09725 at 2188.290 kb on + strand, within LRK53_RS09725 at 2188.291 kb on - strand, within LRK53_RS09725 at 2188.291 kb on - strand, within LRK53_RS09725 at 2188.291 kb on - strand, within LRK53_RS09725 at 2188.291 kb on - strand, within LRK53_RS09725 at 2188.291 kb on - strand, within LRK53_RS09725 at 2188.291 kb on - strand, within LRK53_RS09725 at 2188.342 kb on + strand at 2188.342 kb on + strand at 2188.463 kb on + strand at 2188.463 kb on + strand at 2188.463 kb on + strand at 2188.463 kb on + strand at 2188.463 kb on + strand at 2188.463 kb on + strand at 2188.464 kb on - strand at 2188.464 kb on - strand at 2188.665 kb on + strand, within LRK53_RS09730 at 2188.665 kb on + strand, within LRK53_RS09730 at 2188.784 kb on + strand, within LRK53_RS09730 at 2188.784 kb on + strand, within LRK53_RS09730 at 2188.784 kb on + strand, within LRK53_RS09730 at 2188.785 kb on - strand, within LRK53_RS09730 at 2188.785 kb on - strand, within LRK53_RS09730 at 2188.785 kb on - strand, within LRK53_RS09730 at 2188.785 kb on - strand, within LRK53_RS09730 at 2188.785 kb on - strand, within LRK53_RS09730 at 2188.829 kb on + strand, within LRK53_RS09730 at 2188.829 kb on + strand, within LRK53_RS09730 at 2188.829 kb on + strand, within LRK53_RS09730 at 2188.829 kb on + strand, within LRK53_RS09730 at 2188.829 kb on + strand, within LRK53_RS09730 at 2188.830 kb on - strand, within LRK53_RS09730 at 2188.830 kb on - strand, within LRK53_RS09730 at 2188.830 kb on - strand, within LRK53_RS09730 at 2188.830 kb on - strand, within LRK53_RS09730 at 2189.060 kb on + strand, within LRK53_RS09730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 2,187,997 + +0.3 2,187,997 + +0.5 2,187,997 + +1.6 2,187,997 + +1.6 2,187,997 + -0.1 2,187,997 + +0.4 2,187,997 + -0.5 2,187,997 + -0.3 2,187,997 + -0.2 2,187,997 + +0.4 2,187,997 + +0.2 2,187,997 + -0.7 2,187,997 + -1.6 2,187,997 + +1.2 2,187,997 + +0.3 2,187,998 - +1.2 2,187,998 - +1.2 2,187,998 - +0.9 2,187,998 - -1.1 2,187,998 - -0.4 2,187,998 - +1.2 2,187,998 - -1.4 2,187,998 - -0.2 2,187,998 - -0.8 2,187,998 - -2.0 2,187,998 - -0.4 2,187,998 - +1.2 2,187,998 - -0.3 2,187,998 - -0.4 2,187,998 - -0.1 2,187,998 - -1.5 2,187,998 - -0.8 2,187,999 + -0.4 2,187,999 + +1.2 2,187,999 + +0.0 2,187,999 + -0.4 2,187,999 + -1.1 2,188,000 - +1.2 2,188,125 + LRK53_RS09725 0.29 -0.0 2,188,125 + LRK53_RS09725 0.29 -1.0 2,188,125 + LRK53_RS09725 0.29 -1.0 2,188,125 + LRK53_RS09725 0.29 +1.2 2,188,125 + LRK53_RS09725 0.29 -1.8 2,188,125 + LRK53_RS09725 0.29 -0.1 2,188,125 + LRK53_RS09725 0.29 +1.2 2,188,126 - LRK53_RS09725 0.29 -1.0 2,188,126 - LRK53_RS09725 0.29 -0.4 2,188,126 - LRK53_RS09725 0.29 +2.2 2,188,180 + LRK53_RS09725 0.46 -1.1 2,188,180 + LRK53_RS09725 0.46 -0.4 2,188,180 + LRK53_RS09725 0.46 -1.1 2,188,181 - LRK53_RS09725 0.46 -0.8 2,188,181 - LRK53_RS09725 0.46 -1.8 2,188,181 - LRK53_RS09725 0.46 -0.8 2,188,181 - LRK53_RS09725 0.46 -0.4 2,188,181 - LRK53_RS09725 0.46 +1.2 2,188,181 - LRK53_RS09725 0.46 -0.4 2,188,181 - LRK53_RS09725 0.46 -0.4 2,188,181 - LRK53_RS09725 0.46 -1.4 2,188,181 - LRK53_RS09725 0.46 -1.1 2,188,181 - LRK53_RS09725 0.46 -1.1 2,188,181 - LRK53_RS09725 0.46 -1.1 2,188,234 + LRK53_RS09725 0.62 -1.0 2,188,234 + LRK53_RS09725 0.62 +0.6 2,188,234 + LRK53_RS09725 0.62 +2.2 2,188,251 + LRK53_RS09725 0.67 -0.1 2,188,251 + LRK53_RS09725 0.67 +1.2 2,188,251 + LRK53_RS09725 0.67 -1.4 2,188,251 + LRK53_RS09725 0.67 +0.6 2,188,252 - LRK53_RS09725 0.68 -1.1 2,188,260 + LRK53_RS09725 0.70 +0.6 2,188,260 + LRK53_RS09725 0.70 +0.2 2,188,260 + LRK53_RS09725 0.70 -0.4 2,188,260 + LRK53_RS09725 0.70 -0.8 2,188,260 + LRK53_RS09725 0.70 +0.2 2,188,260 + LRK53_RS09725 0.70 -1.4 2,188,260 + LRK53_RS09725 0.70 +2.2 2,188,260 + LRK53_RS09725 0.70 -0.4 2,188,261 - LRK53_RS09725 0.70 -0.4 2,188,261 - LRK53_RS09725 0.70 -1.1 2,188,261 - LRK53_RS09725 0.70 -1.6 2,188,261 - LRK53_RS09725 0.70 -0.4 2,188,261 - LRK53_RS09725 0.70 -0.6 2,188,261 - LRK53_RS09725 0.70 -0.4 2,188,290 + LRK53_RS09725 0.79 +0.6 2,188,291 - LRK53_RS09725 0.80 +0.2 2,188,291 - LRK53_RS09725 0.80 -1.1 2,188,291 - LRK53_RS09725 0.80 -0.4 2,188,291 - LRK53_RS09725 0.80 +1.2 2,188,291 - LRK53_RS09725 0.80 +2.8 2,188,291 - LRK53_RS09725 0.80 -0.4 2,188,342 + +0.6 2,188,342 + -1.6 2,188,463 + +1.2 2,188,463 + +1.2 2,188,463 + +0.2 2,188,463 + -0.1 2,188,463 + -0.8 2,188,463 + -2.3 2,188,464 - +1.2 2,188,464 - +2.2 2,188,665 + LRK53_RS09730 0.11 +1.2 2,188,665 + LRK53_RS09730 0.11 -0.8 2,188,784 + LRK53_RS09730 0.16 +2.2 2,188,784 + LRK53_RS09730 0.16 +2.2 2,188,784 + LRK53_RS09730 0.16 -1.6 2,188,785 - LRK53_RS09730 0.16 +1.2 2,188,785 - LRK53_RS09730 0.16 +2.2 2,188,785 - LRK53_RS09730 0.16 -0.4 2,188,785 - LRK53_RS09730 0.16 +2.8 2,188,785 - LRK53_RS09730 0.16 +1.2 2,188,829 + LRK53_RS09730 0.18 +0.2 2,188,829 + LRK53_RS09730 0.18 +0.2 2,188,829 + LRK53_RS09730 0.18 +0.2 2,188,829 + LRK53_RS09730 0.18 +1.2 2,188,829 + LRK53_RS09730 0.18 -0.2 2,188,830 - LRK53_RS09730 0.18 +0.2 2,188,830 - LRK53_RS09730 0.18 +1.2 2,188,830 - LRK53_RS09730 0.18 +0.2 2,188,830 - LRK53_RS09730 0.18 -0.7 2,189,060 + LRK53_RS09730 0.28 -0.8
Or see this region's nucleotide sequence