Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09225 and LRK53_RS09230 are separated by 229 nucleotides LRK53_RS09230 and LRK53_RS09235 are separated by 12 nucleotides LRK53_RS09235 and LRK53_RS09240 are separated by 33 nucleotides
LRK53_RS09225: LRK53_RS09225 - adenylosuccinate lyase, at 2,064,404 to 2,065,771
_RS09225
LRK53_RS09230: LRK53_RS09230 - class II fumarate hydratase, at 2,066,001 to 2,067,386
_RS09230
LRK53_RS09235: LRK53_RS09235 - DUF6326 family protein, at 2,067,399 to 2,067,833
_RS09235
LRK53_RS09240: LRK53_RS09240 - DUF4386 domain-containing protein, at 2,067,867 to 2,068,562
_RS09240
Position (kb)
2066
2067
2068 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2065.804 kb on + strand at 2065.804 kb on + strand at 2065.804 kb on + strand at 2065.805 kb on - strand at 2065.805 kb on - strand at 2065.805 kb on - strand at 2065.805 kb on - strand at 2065.836 kb on - strand at 2065.836 kb on - strand at 2065.837 kb on + strand at 2065.838 kb on - strand at 2065.838 kb on - strand at 2066.030 kb on + strand at 2066.030 kb on + strand at 2066.030 kb on + strand at 2066.030 kb on + strand at 2066.030 kb on + strand at 2066.031 kb on - strand at 2066.031 kb on - strand at 2066.041 kb on + strand at 2066.041 kb on + strand at 2066.041 kb on + strand at 2066.041 kb on + strand at 2066.041 kb on + strand at 2066.042 kb on - strand at 2066.042 kb on - strand at 2066.042 kb on - strand at 2066.042 kb on - strand at 2066.042 kb on - strand at 2066.042 kb on - strand at 2066.064 kb on + strand at 2066.064 kb on + strand at 2066.064 kb on + strand at 2066.064 kb on + strand at 2066.065 kb on - strand at 2066.065 kb on - strand at 2066.065 kb on - strand at 2066.065 kb on - strand at 2066.385 kb on + strand, within LRK53_RS09230 at 2066.385 kb on + strand, within LRK53_RS09230 at 2066.385 kb on + strand, within LRK53_RS09230 at 2066.769 kb on + strand, within LRK53_RS09230 at 2066.769 kb on + strand, within LRK53_RS09230 at 2066.769 kb on + strand, within LRK53_RS09230 at 2066.770 kb on - strand, within LRK53_RS09230 at 2067.264 kb on + strand at 2067.264 kb on + strand at 2067.264 kb on + strand at 2067.264 kb on + strand at 2067.265 kb on - strand at 2067.265 kb on - strand at 2067.265 kb on - strand at 2067.265 kb on - strand at 2067.265 kb on - strand at 2067.318 kb on - strand at 2067.318 kb on - strand at 2067.410 kb on + strand at 2067.410 kb on + strand at 2067.410 kb on + strand at 2067.410 kb on + strand at 2067.410 kb on + strand at 2067.410 kb on + strand at 2067.410 kb on + strand at 2067.411 kb on - strand at 2067.411 kb on - strand at 2067.411 kb on - strand at 2067.436 kb on + strand at 2067.436 kb on + strand at 2067.437 kb on - strand at 2067.437 kb on - strand at 2067.437 kb on - strand at 2067.437 kb on - strand at 2067.437 kb on - strand at 2067.487 kb on + strand, within LRK53_RS09235 at 2067.487 kb on + strand, within LRK53_RS09235 at 2067.488 kb on - strand, within LRK53_RS09235 at 2067.488 kb on - strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.535 kb on + strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.536 kb on - strand, within LRK53_RS09235 at 2067.715 kb on + strand, within LRK53_RS09235 at 2067.715 kb on + strand, within LRK53_RS09235 at 2067.716 kb on - strand, within LRK53_RS09235 at 2067.716 kb on - strand, within LRK53_RS09235 at 2067.727 kb on + strand, within LRK53_RS09235 at 2067.727 kb on + strand, within LRK53_RS09235 at 2067.736 kb on + strand, within LRK53_RS09235 at 2067.736 kb on + strand, within LRK53_RS09235 at 2067.736 kb on + strand, within LRK53_RS09235 at 2067.736 kb on + strand, within LRK53_RS09235 at 2067.736 kb on + strand, within LRK53_RS09235 at 2067.736 kb on + strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.737 kb on - strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.742 kb on + strand, within LRK53_RS09235 at 2067.743 kb on - strand, within LRK53_RS09235 at 2067.743 kb on - strand, within LRK53_RS09235 at 2067.743 kb on - strand, within LRK53_RS09235 at 2067.847 kb on + strand at 2068.042 kb on + strand, within LRK53_RS09240 at 2068.042 kb on + strand, within LRK53_RS09240 at 2068.043 kb on - strand, within LRK53_RS09240 at 2068.043 kb on - strand, within LRK53_RS09240 at 2068.288 kb on + strand, within LRK53_RS09240 at 2068.289 kb on - strand, within LRK53_RS09240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 2,065,804 + -0.4 2,065,804 + -1.1 2,065,804 + +0.6 2,065,805 - -0.1 2,065,805 - -0.8 2,065,805 - +0.2 2,065,805 - +1.2 2,065,836 - -0.6 2,065,836 - +0.7 2,065,837 + +0.2 2,065,838 - -0.1 2,065,838 - +0.1 2,066,030 + +1.2 2,066,030 + -0.4 2,066,030 + +0.5 2,066,030 + -2.0 2,066,030 + -0.4 2,066,031 - -0.8 2,066,031 - -2.0 2,066,041 + -0.8 2,066,041 + -0.8 2,066,041 + +0.6 2,066,041 + -0.4 2,066,041 + +1.2 2,066,042 - +0.9 2,066,042 - +0.2 2,066,042 - +0.2 2,066,042 - -1.1 2,066,042 - +0.9 2,066,042 - +0.5 2,066,064 + +2.8 2,066,064 + -2.1 2,066,064 + +1.2 2,066,064 + -1.4 2,066,065 - +1.2 2,066,065 - +0.2 2,066,065 - -0.8 2,066,065 - -1.6 2,066,385 + LRK53_RS09230 0.28 +0.5 2,066,385 + LRK53_RS09230 0.28 +0.2 2,066,385 + LRK53_RS09230 0.28 -3.1 2,066,769 + LRK53_RS09230 0.55 -1.8 2,066,769 + LRK53_RS09230 0.55 -1.6 2,066,769 + LRK53_RS09230 0.55 -0.0 2,066,770 - LRK53_RS09230 0.55 -0.8 2,067,264 + -0.8 2,067,264 + -0.8 2,067,264 + -0.4 2,067,264 + -0.1 2,067,265 - -1.1 2,067,265 - -1.0 2,067,265 - +1.2 2,067,265 - +0.8 2,067,265 - -1.4 2,067,318 - +0.2 2,067,318 - +0.6 2,067,410 + +0.6 2,067,410 + -0.3 2,067,410 + +1.2 2,067,410 + -1.8 2,067,410 + +0.2 2,067,410 + -0.1 2,067,410 + -0.6 2,067,411 - -1.0 2,067,411 - -0.0 2,067,411 - -1.4 2,067,436 + +0.2 2,067,436 + +0.2 2,067,437 - -0.3 2,067,437 - +0.2 2,067,437 - -0.8 2,067,437 - -0.2 2,067,437 - -0.3 2,067,487 + LRK53_RS09235 0.20 -0.4 2,067,487 + LRK53_RS09235 0.20 +1.2 2,067,488 - LRK53_RS09235 0.20 -1.1 2,067,488 - LRK53_RS09235 0.20 -0.4 2,067,535 + LRK53_RS09235 0.31 -0.7 2,067,535 + LRK53_RS09235 0.31 -2.3 2,067,535 + LRK53_RS09235 0.31 +0.6 2,067,535 + LRK53_RS09235 0.31 -0.6 2,067,535 + LRK53_RS09235 0.31 +0.0 2,067,535 + LRK53_RS09235 0.31 -0.4 2,067,535 + LRK53_RS09235 0.31 -1.1 2,067,535 + LRK53_RS09235 0.31 -0.6 2,067,535 + LRK53_RS09235 0.31 -0.4 2,067,536 - LRK53_RS09235 0.31 +1.2 2,067,536 - LRK53_RS09235 0.31 -0.4 2,067,536 - LRK53_RS09235 0.31 -0.6 2,067,536 - LRK53_RS09235 0.31 -0.1 2,067,536 - LRK53_RS09235 0.31 -0.1 2,067,536 - LRK53_RS09235 0.31 +0.2 2,067,536 - LRK53_RS09235 0.31 +1.2 2,067,536 - LRK53_RS09235 0.31 +0.2 2,067,536 - LRK53_RS09235 0.31 -1.1 2,067,536 - LRK53_RS09235 0.31 -0.4 2,067,536 - LRK53_RS09235 0.31 -0.8 2,067,536 - LRK53_RS09235 0.31 +2.2 2,067,715 + LRK53_RS09235 0.73 +1.2 2,067,715 + LRK53_RS09235 0.73 +2.8 2,067,716 - LRK53_RS09235 0.73 -1.3 2,067,716 - LRK53_RS09235 0.73 -0.8 2,067,727 + LRK53_RS09235 0.75 -0.0 2,067,727 + LRK53_RS09235 0.75 -0.9 2,067,736 + LRK53_RS09235 0.77 +0.0 2,067,736 + LRK53_RS09235 0.77 +1.2 2,067,736 + LRK53_RS09235 0.77 -0.6 2,067,736 + LRK53_RS09235 0.77 -0.0 2,067,736 + LRK53_RS09235 0.77 -1.0 2,067,736 + LRK53_RS09235 0.77 +0.6 2,067,737 - LRK53_RS09235 0.78 +0.2 2,067,737 - LRK53_RS09235 0.78 -0.1 2,067,737 - LRK53_RS09235 0.78 -0.1 2,067,737 - LRK53_RS09235 0.78 -1.6 2,067,737 - LRK53_RS09235 0.78 -1.7 2,067,737 - LRK53_RS09235 0.78 +0.2 2,067,737 - LRK53_RS09235 0.78 +0.5 2,067,737 - LRK53_RS09235 0.78 +0.2 2,067,737 - LRK53_RS09235 0.78 +0.5 2,067,737 - LRK53_RS09235 0.78 +0.7 2,067,742 + LRK53_RS09235 0.79 -1.6 2,067,742 + LRK53_RS09235 0.79 -1.1 2,067,742 + LRK53_RS09235 0.79 -1.1 2,067,742 + LRK53_RS09235 0.79 -0.8 2,067,742 + LRK53_RS09235 0.79 +1.2 2,067,742 + LRK53_RS09235 0.79 +0.2 2,067,742 + LRK53_RS09235 0.79 +0.2 2,067,742 + LRK53_RS09235 0.79 -0.4 2,067,743 - LRK53_RS09235 0.79 -0.8 2,067,743 - LRK53_RS09235 0.79 +0.2 2,067,743 - LRK53_RS09235 0.79 -0.4 2,067,847 + +0.2 2,068,042 + LRK53_RS09240 0.25 -0.4 2,068,042 + LRK53_RS09240 0.25 +0.2 2,068,043 - LRK53_RS09240 0.25 -1.0 2,068,043 - LRK53_RS09240 0.25 -1.1 2,068,288 + LRK53_RS09240 0.60 +0.6 2,068,289 - LRK53_RS09240 0.61 -1.1
Or see this region's nucleotide sequence