Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08215 and LRK53_RS08220 overlap by 4 nucleotides LRK53_RS08220 and LRK53_RS08225 overlap by 8 nucleotides LRK53_RS08225 and LRK53_RS08230 are separated by 69 nucleotides LRK53_RS08230 and LRK53_RS08235 are separated by 157 nucleotides
LRK53_RS08215: LRK53_RS08215 - MFS transporter, at 1,850,215 to 1,851,336
_RS08215
LRK53_RS08220: LRK53_RS08220 - HAD family phosphatase, at 1,851,333 to 1,851,962
_RS08220
LRK53_RS08225: LRK53_RS08225 - DeoR/GlpR family DNA-binding transcription regulator, at 1,851,955 to 1,852,752
_RS08225
LRK53_RS08230: LRK53_RS08230 - DUF2147 domain-containing protein, at 1,852,822 to 1,853,286
_RS08230
LRK53_RS08235: LRK53_RS08235 - iron-sulfur cluster carrier protein ApbC, at 1,853,444 to 1,854,538
_RS08235
Position (kb)
1851
1852
1853 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1851.095 kb on + strand, within LRK53_RS08215 at 1851.095 kb on + strand, within LRK53_RS08215 at 1851.095 kb on + strand, within LRK53_RS08215 at 1851.096 kb on - strand, within LRK53_RS08215 at 1851.096 kb on - strand, within LRK53_RS08215 at 1851.096 kb on - strand, within LRK53_RS08215 at 1851.205 kb on + strand, within LRK53_RS08215 at 1851.205 kb on + strand, within LRK53_RS08215 at 1851.408 kb on + strand, within LRK53_RS08220 at 1851.409 kb on - strand, within LRK53_RS08220 at 1851.657 kb on + strand, within LRK53_RS08220 at 1851.658 kb on - strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.831 kb on + strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.832 kb on - strand, within LRK53_RS08220 at 1851.960 kb on + strand at 1851.961 kb on - strand at 1851.961 kb on - strand at 1852.138 kb on + strand, within LRK53_RS08225 at 1852.139 kb on - strand, within LRK53_RS08225 at 1852.614 kb on + strand, within LRK53_RS08225 at 1852.614 kb on + strand, within LRK53_RS08225 at 1852.874 kb on + strand, within LRK53_RS08230 at 1852.874 kb on + strand, within LRK53_RS08230 at 1852.874 kb on + strand, within LRK53_RS08230 at 1852.874 kb on + strand, within LRK53_RS08230 at 1852.874 kb on + strand, within LRK53_RS08230 at 1852.874 kb on + strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.875 kb on - strand, within LRK53_RS08230 at 1852.928 kb on + strand, within LRK53_RS08230 at 1852.928 kb on + strand, within LRK53_RS08230 at 1852.928 kb on + strand, within LRK53_RS08230 at 1852.928 kb on + strand, within LRK53_RS08230 at 1852.928 kb on + strand, within LRK53_RS08230 at 1852.929 kb on - strand, within LRK53_RS08230 at 1852.929 kb on - strand, within LRK53_RS08230 at 1852.929 kb on - strand, within LRK53_RS08230 at 1852.929 kb on - strand, within LRK53_RS08230 at 1853.126 kb on + strand, within LRK53_RS08230 at 1853.126 kb on + strand, within LRK53_RS08230 at 1853.126 kb on + strand, within LRK53_RS08230 at 1853.126 kb on + strand, within LRK53_RS08230 at 1853.126 kb on + strand, within LRK53_RS08230 at 1853.127 kb on - strand, within LRK53_RS08230 at 1853.127 kb on - strand, within LRK53_RS08230 at 1853.127 kb on - strand, within LRK53_RS08230 at 1853.127 kb on - strand, within LRK53_RS08230 at 1853.310 kb on + strand at 1853.310 kb on + strand at 1853.310 kb on + strand at 1853.409 kb on + strand at 1853.409 kb on + strand at 1853.409 kb on + strand at 1853.409 kb on + strand at 1853.410 kb on - strand at 1853.410 kb on - strand at 1853.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,851,095 + LRK53_RS08215 0.78 +2.2 1,851,095 + LRK53_RS08215 0.78 -1.8 1,851,095 + LRK53_RS08215 0.78 +0.0 1,851,096 - LRK53_RS08215 0.79 +0.1 1,851,096 - LRK53_RS08215 0.79 -0.1 1,851,096 - LRK53_RS08215 0.79 +0.9 1,851,205 + LRK53_RS08215 0.88 -0.8 1,851,205 + LRK53_RS08215 0.88 -2.0 1,851,408 + LRK53_RS08220 0.12 -0.4 1,851,409 - LRK53_RS08220 0.12 +0.1 1,851,657 + LRK53_RS08220 0.51 -1.4 1,851,658 - LRK53_RS08220 0.52 -0.6 1,851,831 + LRK53_RS08220 0.79 +0.9 1,851,831 + LRK53_RS08220 0.79 -0.8 1,851,831 + LRK53_RS08220 0.79 +0.2 1,851,831 + LRK53_RS08220 0.79 -2.2 1,851,831 + LRK53_RS08220 0.79 -0.8 1,851,831 + LRK53_RS08220 0.79 -1.3 1,851,831 + LRK53_RS08220 0.79 -1.0 1,851,831 + LRK53_RS08220 0.79 +0.4 1,851,831 + LRK53_RS08220 0.79 -1.2 1,851,831 + LRK53_RS08220 0.79 +0.2 1,851,831 + LRK53_RS08220 0.79 -0.9 1,851,831 + LRK53_RS08220 0.79 -0.4 1,851,832 - LRK53_RS08220 0.79 -0.0 1,851,832 - LRK53_RS08220 0.79 +0.2 1,851,832 - LRK53_RS08220 0.79 -1.2 1,851,832 - LRK53_RS08220 0.79 -0.4 1,851,832 - LRK53_RS08220 0.79 -0.1 1,851,832 - LRK53_RS08220 0.79 +2.2 1,851,832 - LRK53_RS08220 0.79 -1.0 1,851,832 - LRK53_RS08220 0.79 -0.9 1,851,832 - LRK53_RS08220 0.79 +0.1 1,851,832 - LRK53_RS08220 0.79 -0.4 1,851,832 - LRK53_RS08220 0.79 -1.4 1,851,832 - LRK53_RS08220 0.79 -0.8 1,851,832 - LRK53_RS08220 0.79 +0.2 1,851,832 - LRK53_RS08220 0.79 -0.1 1,851,832 - LRK53_RS08220 0.79 -0.4 1,851,960 + +0.2 1,851,961 - +0.2 1,851,961 - -1.3 1,852,138 + LRK53_RS08225 0.23 -1.2 1,852,139 - LRK53_RS08225 0.23 -2.6 1,852,614 + LRK53_RS08225 0.83 -0.4 1,852,614 + LRK53_RS08225 0.83 -1.1 1,852,874 + LRK53_RS08230 0.11 -0.1 1,852,874 + LRK53_RS08230 0.11 -0.1 1,852,874 + LRK53_RS08230 0.11 -1.1 1,852,874 + LRK53_RS08230 0.11 -0.3 1,852,874 + LRK53_RS08230 0.11 +0.5 1,852,874 + LRK53_RS08230 0.11 +1.2 1,852,875 - LRK53_RS08230 0.11 +0.2 1,852,875 - LRK53_RS08230 0.11 +0.6 1,852,875 - LRK53_RS08230 0.11 +1.2 1,852,875 - LRK53_RS08230 0.11 -0.1 1,852,875 - LRK53_RS08230 0.11 +1.2 1,852,875 - LRK53_RS08230 0.11 -1.1 1,852,875 - LRK53_RS08230 0.11 -1.4 1,852,875 - LRK53_RS08230 0.11 -0.4 1,852,875 - LRK53_RS08230 0.11 -1.0 1,852,928 + LRK53_RS08230 0.23 -0.4 1,852,928 + LRK53_RS08230 0.23 +1.2 1,852,928 + LRK53_RS08230 0.23 -0.8 1,852,928 + LRK53_RS08230 0.23 +0.2 1,852,928 + LRK53_RS08230 0.23 +0.2 1,852,929 - LRK53_RS08230 0.23 +2.2 1,852,929 - LRK53_RS08230 0.23 +0.4 1,852,929 - LRK53_RS08230 0.23 +1.2 1,852,929 - LRK53_RS08230 0.23 +0.2 1,853,126 + LRK53_RS08230 0.65 +0.6 1,853,126 + LRK53_RS08230 0.65 -0.4 1,853,126 + LRK53_RS08230 0.65 -1.5 1,853,126 + LRK53_RS08230 0.65 +1.2 1,853,126 + LRK53_RS08230 0.65 +0.2 1,853,127 - LRK53_RS08230 0.66 -0.4 1,853,127 - LRK53_RS08230 0.66 +0.2 1,853,127 - LRK53_RS08230 0.66 -0.1 1,853,127 - LRK53_RS08230 0.66 +1.2 1,853,310 + +0.4 1,853,310 + +1.2 1,853,310 + -1.7 1,853,409 + -0.8 1,853,409 + -0.4 1,853,409 + -0.1 1,853,409 + +2.2 1,853,410 - -2.0 1,853,410 - -0.8 1,853,410 - -0.8
Or see this region's nucleotide sequence