Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08195 and LRK53_RS08200 are separated by 148 nucleotides LRK53_RS08200 and LRK53_RS08205 are separated by 121 nucleotides LRK53_RS08205 and LRK53_RS08210 are separated by 94 nucleotides
LRK53_RS08195: LRK53_RS08195 - class I SAM-dependent methyltransferase, at 1,845,645 to 1,846,253
_RS08195
LRK53_RS08200: LRK53_RS08200 - endonuclease III, at 1,846,402 to 1,847,037
_RS08200
LRK53_RS08205: LRK53_RS08205 - RnfABCDGE type electron transport complex subunit B, at 1,847,159 to 1,847,761
_RS08205
LRK53_RS08210: LRK53_RS08210 - methionine--tRNA ligase, at 1,847,856 to 1,849,982
_RS08210
Position (kb)
1847
1848 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1846.191 kb on + strand, within LRK53_RS08195 at 1846.191 kb on + strand, within LRK53_RS08195 at 1846.192 kb on - strand, within LRK53_RS08195 at 1846.192 kb on - strand, within LRK53_RS08195 at 1846.275 kb on - strand at 1846.275 kb on - strand at 1846.275 kb on - strand at 1846.275 kb on - strand at 1846.369 kb on + strand at 1846.369 kb on + strand at 1846.404 kb on - strand at 1846.424 kb on + strand at 1846.424 kb on + strand at 1846.424 kb on + strand at 1846.424 kb on + strand at 1846.424 kb on + strand at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.480 kb on + strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.481 kb on - strand, within LRK53_RS08200 at 1846.484 kb on + strand, within LRK53_RS08200 at 1846.484 kb on + strand, within LRK53_RS08200 at 1846.484 kb on + strand, within LRK53_RS08200 at 1846.663 kb on + strand, within LRK53_RS08200 at 1846.663 kb on + strand, within LRK53_RS08200 at 1846.663 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.814 kb on + strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.815 kb on - strand, within LRK53_RS08200 at 1846.874 kb on + strand, within LRK53_RS08200 at 1847.679 kb on + strand, within LRK53_RS08205 at 1847.679 kb on + strand, within LRK53_RS08205 at 1847.680 kb on - strand, within LRK53_RS08205 at 1847.782 kb on - strand at 1847.782 kb on - strand at 1847.782 kb on - strand at 1847.782 kb on - strand at 1847.788 kb on + strand at 1847.789 kb on - strand at 1847.789 kb on - strand at 1847.789 kb on - strand at 1847.789 kb on - strand at 1847.825 kb on + strand at 1847.826 kb on - strand at 1847.826 kb on - strand at 1847.826 kb on - strand at 1847.832 kb on + strand at 1847.833 kb on - strand at 1847.839 kb on + strand at 1847.840 kb on - strand at 1847.840 kb on - strand at 1847.840 kb on - strand at 1847.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,846,191 + LRK53_RS08195 0.90 +1.2 1,846,191 + LRK53_RS08195 0.90 +1.8 1,846,192 - LRK53_RS08195 0.90 -0.6 1,846,192 - LRK53_RS08195 0.90 -2.4 1,846,275 - -1.4 1,846,275 - +0.8 1,846,275 - +0.5 1,846,275 - -0.8 1,846,369 + +0.2 1,846,369 + -0.5 1,846,404 - -0.4 1,846,424 + -1.3 1,846,424 + -1.8 1,846,424 + -0.6 1,846,424 + -1.0 1,846,424 + +0.1 1,846,480 + LRK53_RS08200 0.12 -1.5 1,846,480 + LRK53_RS08200 0.12 -0.2 1,846,480 + LRK53_RS08200 0.12 -0.4 1,846,480 + LRK53_RS08200 0.12 +1.2 1,846,480 + LRK53_RS08200 0.12 -0.1 1,846,480 + LRK53_RS08200 0.12 -0.4 1,846,480 + LRK53_RS08200 0.12 +2.2 1,846,480 + LRK53_RS08200 0.12 +0.6 1,846,480 + LRK53_RS08200 0.12 -0.4 1,846,480 + LRK53_RS08200 0.12 +1.2 1,846,480 + LRK53_RS08200 0.12 +0.8 1,846,480 + LRK53_RS08200 0.12 -0.1 1,846,480 + LRK53_RS08200 0.12 -0.8 1,846,480 + LRK53_RS08200 0.12 +0.2 1,846,480 + LRK53_RS08200 0.12 +0.8 1,846,480 + LRK53_RS08200 0.12 -0.8 1,846,480 + LRK53_RS08200 0.12 +0.2 1,846,480 + LRK53_RS08200 0.12 -0.8 1,846,480 + LRK53_RS08200 0.12 -0.8 1,846,480 + LRK53_RS08200 0.12 +1.2 1,846,480 + LRK53_RS08200 0.12 +3.5 1,846,480 + LRK53_RS08200 0.12 -0.4 1,846,480 + LRK53_RS08200 0.12 +0.2 1,846,480 + LRK53_RS08200 0.12 -0.0 1,846,480 + LRK53_RS08200 0.12 -0.1 1,846,480 + LRK53_RS08200 0.12 +1.2 1,846,480 + LRK53_RS08200 0.12 -1.1 1,846,480 + LRK53_RS08200 0.12 +0.2 1,846,481 - LRK53_RS08200 0.12 -0.8 1,846,481 - LRK53_RS08200 0.12 +0.6 1,846,481 - LRK53_RS08200 0.12 -1.1 1,846,481 - LRK53_RS08200 0.12 -0.6 1,846,481 - LRK53_RS08200 0.12 -0.6 1,846,481 - LRK53_RS08200 0.12 +1.2 1,846,481 - LRK53_RS08200 0.12 -0.4 1,846,481 - LRK53_RS08200 0.12 +0.5 1,846,481 - LRK53_RS08200 0.12 +0.6 1,846,481 - LRK53_RS08200 0.12 -2.1 1,846,481 - LRK53_RS08200 0.12 -1.2 1,846,481 - LRK53_RS08200 0.12 +0.8 1,846,481 - LRK53_RS08200 0.12 +0.2 1,846,481 - LRK53_RS08200 0.12 +1.2 1,846,481 - LRK53_RS08200 0.12 -0.2 1,846,481 - LRK53_RS08200 0.12 -0.6 1,846,481 - LRK53_RS08200 0.12 -0.4 1,846,481 - LRK53_RS08200 0.12 -0.8 1,846,481 - LRK53_RS08200 0.12 -0.6 1,846,481 - LRK53_RS08200 0.12 -1.1 1,846,481 - LRK53_RS08200 0.12 -0.6 1,846,481 - LRK53_RS08200 0.12 +0.5 1,846,481 - LRK53_RS08200 0.12 -1.4 1,846,481 - LRK53_RS08200 0.12 +1.2 1,846,481 - LRK53_RS08200 0.12 -0.4 1,846,481 - LRK53_RS08200 0.12 +0.7 1,846,481 - LRK53_RS08200 0.12 +0.2 1,846,481 - LRK53_RS08200 0.12 +0.5 1,846,484 + LRK53_RS08200 0.13 +2.2 1,846,484 + LRK53_RS08200 0.13 -1.6 1,846,484 + LRK53_RS08200 0.13 -0.5 1,846,663 + LRK53_RS08200 0.41 -0.9 1,846,663 + LRK53_RS08200 0.41 +0.6 1,846,663 + LRK53_RS08200 0.41 -0.7 1,846,814 + LRK53_RS08200 0.65 -0.6 1,846,814 + LRK53_RS08200 0.65 -0.4 1,846,814 + LRK53_RS08200 0.65 -0.4 1,846,814 + LRK53_RS08200 0.65 -0.4 1,846,814 + LRK53_RS08200 0.65 -1.8 1,846,814 + LRK53_RS08200 0.65 -0.5 1,846,814 + LRK53_RS08200 0.65 -0.8 1,846,814 + LRK53_RS08200 0.65 +0.6 1,846,814 + LRK53_RS08200 0.65 +0.5 1,846,814 + LRK53_RS08200 0.65 -1.1 1,846,814 + LRK53_RS08200 0.65 +0.2 1,846,815 - LRK53_RS08200 0.65 -0.2 1,846,815 - LRK53_RS08200 0.65 +1.0 1,846,815 - LRK53_RS08200 0.65 -0.8 1,846,815 - LRK53_RS08200 0.65 -1.1 1,846,815 - LRK53_RS08200 0.65 -0.8 1,846,815 - LRK53_RS08200 0.65 +1.2 1,846,815 - LRK53_RS08200 0.65 +0.2 1,846,815 - LRK53_RS08200 0.65 -0.4 1,846,815 - LRK53_RS08200 0.65 -2.1 1,846,815 - LRK53_RS08200 0.65 -0.8 1,846,815 - LRK53_RS08200 0.65 -0.1 1,846,815 - LRK53_RS08200 0.65 -0.8 1,846,815 - LRK53_RS08200 0.65 +0.6 1,846,874 + LRK53_RS08200 0.74 -1.1 1,847,679 + LRK53_RS08205 0.86 -0.7 1,847,679 + LRK53_RS08205 0.86 -0.8 1,847,680 - LRK53_RS08205 0.86 -1.6 1,847,782 - +1.9 1,847,782 - -0.8 1,847,782 - +0.4 1,847,782 - -2.4 1,847,788 + +0.2 1,847,789 - -0.8 1,847,789 - -0.6 1,847,789 - -1.2 1,847,789 - +1.2 1,847,825 + -1.6 1,847,826 - -0.6 1,847,826 - +0.2 1,847,826 - -0.8 1,847,832 + +1.2 1,847,833 - -0.8 1,847,839 + +0.1 1,847,840 - -0.8 1,847,840 - +0.8 1,847,840 - -1.0 1,847,840 - +0.2
Or see this region's nucleotide sequence