Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07460 and LRK53_RS07465 are separated by 117 nucleotides LRK53_RS07465 and LRK53_RS07470 are separated by 232 nucleotides LRK53_RS07470 and LRK53_RS07475 are separated by 14 nucleotides LRK53_RS07475 and LRK53_RS07480 are separated by 78 nucleotides
LRK53_RS07460: LRK53_RS07460 - EF-P beta-lysylation protein EpmB, at 1,669,057 to 1,670,073
_RS07460
LRK53_RS07465: LRK53_RS07465 - elongation factor P, at 1,670,191 to 1,670,757
_RS07465
LRK53_RS07470: LRK53_RS07470 - TRZ/ATZ family hydrolase, at 1,670,990 to 1,672,321
_RS07470
LRK53_RS07475: LRK53_RS07475 - YciI family protein, at 1,672,336 to 1,672,590
_RS07475
LRK53_RS07480: LRK53_RS07480 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 1,672,669 to 1,673,397
_RS07480
Position (kb)
1670
1671
1672
1673 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.890 kb on + strand at 1670.891 kb on - strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.967 kb on - strand at 1671.147 kb on - strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.307 kb on - strand, within LRK53_RS07470 at 1671.307 kb on - strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.422 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.504 kb on - strand, within LRK53_RS07470 at 1671.504 kb on - strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.584 kb on + strand, within LRK53_RS07470 at 1671.584 kb on + strand, within LRK53_RS07470 at 1671.584 kb on + strand, within LRK53_RS07470 at 1671.585 kb on - strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1672.032 kb on + strand, within LRK53_RS07470 at 1672.032 kb on + strand, within LRK53_RS07470 at 1672.033 kb on - strand, within LRK53_RS07470 at 1672.033 kb on - strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.264 kb on + strand at 1672.345 kb on + strand at 1672.346 kb on - strand at 1672.346 kb on - strand at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.571 kb on - strand at 1672.571 kb on - strand at 1672.571 kb on - strand at 1672.571 kb on - strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.640 kb on - strand at 1672.640 kb on - strand at 1672.640 kb on - strand at 1672.640 kb on - strand at 1672.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,670,113 + -1.8 1,670,113 + -1.5 1,670,113 + +0.1 1,670,113 + +0.7 1,670,113 + +1.2 1,670,113 + -1.5 1,670,113 + -0.5 1,670,113 + -0.9 1,670,173 + +1.2 1,670,173 + +2.8 1,670,173 + +1.2 1,670,173 + -0.8 1,670,173 + -0.8 1,670,173 + -1.0 1,670,173 + +0.2 1,670,173 + -0.3 1,670,173 + +0.8 1,670,173 + +0.2 1,670,175 + -1.6 1,670,175 + -0.2 1,670,175 + -0.3 1,670,175 + +0.2 1,670,175 + -1.9 1,670,175 + -0.8 1,670,175 + -0.3 1,670,175 + +0.7 1,670,175 + -0.8 1,670,175 + -0.2 1,670,830 + -2.1 1,670,830 + +0.2 1,670,830 + -0.3 1,670,830 + -0.3 1,670,830 + -0.3 1,670,830 + -1.1 1,670,830 + +0.2 1,670,830 + +0.8 1,670,830 + -2.2 1,670,830 + +0.0 1,670,831 - +2.2 1,670,831 - -1.3 1,670,831 - +1.2 1,670,831 - -1.1 1,670,831 - -0.6 1,670,831 - -1.9 1,670,831 - -0.3 1,670,890 + -0.5 1,670,891 - -0.1 1,670,962 + -0.7 1,670,962 + -1.7 1,670,962 + +0.2 1,670,962 + +0.2 1,670,962 + -0.8 1,670,962 + -0.1 1,670,962 + +0.2 1,670,962 + -1.8 1,670,962 + +1.2 1,670,962 + -1.7 1,670,962 + -0.3 1,670,962 + -1.6 1,670,962 + -0.3 1,670,962 + +1.2 1,670,962 + -2.0 1,670,962 + +0.6 1,670,963 - -1.3 1,670,963 - +1.2 1,670,963 - +0.2 1,670,963 - +1.2 1,670,963 - -2.2 1,670,963 - +0.0 1,670,963 - -2.6 1,670,963 - -0.4 1,670,963 - +0.7 1,670,963 - -0.8 1,670,966 + +0.4 1,670,966 + -1.6 1,670,966 + +0.2 1,670,966 + -0.3 1,670,966 + -0.1 1,670,966 + +0.2 1,670,966 + -0.1 1,670,966 + +0.2 1,670,966 + +0.2 1,670,967 - +0.7 1,671,147 - LRK53_RS07470 0.12 +1.2 1,671,306 + LRK53_RS07470 0.24 -1.1 1,671,306 + LRK53_RS07470 0.24 -1.6 1,671,306 + LRK53_RS07470 0.24 -1.1 1,671,306 + LRK53_RS07470 0.24 +0.7 1,671,306 + LRK53_RS07470 0.24 -1.3 1,671,306 + LRK53_RS07470 0.24 -0.8 1,671,306 + LRK53_RS07470 0.24 +1.2 1,671,306 + LRK53_RS07470 0.24 +0.7 1,671,307 - LRK53_RS07470 0.24 -1.3 1,671,307 - LRK53_RS07470 0.24 -0.3 1,671,392 + LRK53_RS07470 0.30 +0.2 1,671,392 + LRK53_RS07470 0.30 -0.8 1,671,392 + LRK53_RS07470 0.30 -0.2 1,671,392 + LRK53_RS07470 0.30 -1.9 1,671,392 + LRK53_RS07470 0.30 +0.2 1,671,392 + LRK53_RS07470 0.30 -0.3 1,671,392 + LRK53_RS07470 0.30 -1.2 1,671,392 + LRK53_RS07470 0.30 +0.2 1,671,392 + LRK53_RS07470 0.30 -1.3 1,671,392 + LRK53_RS07470 0.30 -0.1 1,671,392 + LRK53_RS07470 0.30 +1.2 1,671,393 - LRK53_RS07470 0.30 +1.2 1,671,393 - LRK53_RS07470 0.30 +0.2 1,671,393 - LRK53_RS07470 0.30 -1.8 1,671,393 - LRK53_RS07470 0.30 +1.2 1,671,393 - LRK53_RS07470 0.30 -1.1 1,671,393 - LRK53_RS07470 0.30 +0.2 1,671,393 - LRK53_RS07470 0.30 -0.8 1,671,422 + LRK53_RS07470 0.32 -1.1 1,671,503 + LRK53_RS07470 0.39 -0.8 1,671,503 + LRK53_RS07470 0.39 -0.3 1,671,503 + LRK53_RS07470 0.39 +1.2 1,671,503 + LRK53_RS07470 0.39 -0.3 1,671,503 + LRK53_RS07470 0.39 +1.2 1,671,504 - LRK53_RS07470 0.39 +1.2 1,671,504 - LRK53_RS07470 0.39 -1.1 1,671,527 + LRK53_RS07470 0.40 +0.7 1,671,527 + LRK53_RS07470 0.40 -0.8 1,671,527 + LRK53_RS07470 0.40 +1.2 1,671,527 + LRK53_RS07470 0.40 +0.2 1,671,527 + LRK53_RS07470 0.40 +0.2 1,671,527 + LRK53_RS07470 0.40 -0.3 1,671,527 + LRK53_RS07470 0.40 -1.2 1,671,527 + LRK53_RS07470 0.40 +1.0 1,671,527 + LRK53_RS07470 0.40 -0.6 1,671,528 - LRK53_RS07470 0.40 -1.1 1,671,528 - LRK53_RS07470 0.40 -1.1 1,671,528 - LRK53_RS07470 0.40 -0.3 1,671,528 - LRK53_RS07470 0.40 -0.8 1,671,528 - LRK53_RS07470 0.40 -0.3 1,671,584 + LRK53_RS07470 0.45 +0.4 1,671,584 + LRK53_RS07470 0.45 -0.8 1,671,584 + LRK53_RS07470 0.45 -0.8 1,671,585 - LRK53_RS07470 0.45 +0.7 1,671,586 + LRK53_RS07470 0.45 +1.2 1,671,586 + LRK53_RS07470 0.45 -1.1 1,671,586 + LRK53_RS07470 0.45 -1.5 1,671,586 + LRK53_RS07470 0.45 +0.2 1,671,586 + LRK53_RS07470 0.45 +0.2 1,671,586 + LRK53_RS07470 0.45 +1.2 1,671,586 + LRK53_RS07470 0.45 +1.2 1,671,586 + LRK53_RS07470 0.45 -0.9 1,671,586 + LRK53_RS07470 0.45 +1.8 1,671,586 + LRK53_RS07470 0.45 -0.3 1,671,586 + LRK53_RS07470 0.45 +1.8 1,671,586 + LRK53_RS07470 0.45 -1.3 1,671,586 + LRK53_RS07470 0.45 -3.1 1,671,587 - LRK53_RS07470 0.45 -0.8 1,671,587 - LRK53_RS07470 0.45 +0.2 1,671,587 - LRK53_RS07470 0.45 -1.5 1,671,587 - LRK53_RS07470 0.45 -0.3 1,671,587 - LRK53_RS07470 0.45 -0.8 1,671,587 - LRK53_RS07470 0.45 -2.2 1,671,587 - LRK53_RS07470 0.45 -0.2 1,671,587 - LRK53_RS07470 0.45 +0.2 1,671,587 - LRK53_RS07470 0.45 +0.2 1,671,587 - LRK53_RS07470 0.45 +0.7 1,671,587 - LRK53_RS07470 0.45 -0.8 1,671,587 - LRK53_RS07470 0.45 -0.3 1,672,032 + LRK53_RS07470 0.78 +1.2 1,672,032 + LRK53_RS07470 0.78 +1.2 1,672,033 - LRK53_RS07470 0.78 -0.3 1,672,033 - LRK53_RS07470 0.78 +1.2 1,672,181 + LRK53_RS07470 0.89 +0.7 1,672,181 + LRK53_RS07470 0.89 +0.2 1,672,181 + LRK53_RS07470 0.89 +1.2 1,672,181 + LRK53_RS07470 0.89 +0.2 1,672,181 + LRK53_RS07470 0.89 -0.3 1,672,181 + LRK53_RS07470 0.89 +1.8 1,672,181 + LRK53_RS07470 0.89 +1.2 1,672,181 + LRK53_RS07470 0.89 +2.2 1,672,182 - LRK53_RS07470 0.89 -0.8 1,672,182 - LRK53_RS07470 0.89 -1.1 1,672,182 - LRK53_RS07470 0.89 -0.5 1,672,182 - LRK53_RS07470 0.89 -0.3 1,672,182 - LRK53_RS07470 0.89 -1.1 1,672,182 - LRK53_RS07470 0.89 +1.2 1,672,182 - LRK53_RS07470 0.89 +1.2 1,672,264 + -0.3 1,672,345 + -0.2 1,672,346 - -1.0 1,672,346 - -1.0 1,672,480 + LRK53_RS07475 0.56 -0.4 1,672,480 + LRK53_RS07475 0.56 -1.4 1,672,480 + LRK53_RS07475 0.56 -1.3 1,672,480 + LRK53_RS07475 0.56 -1.1 1,672,480 + LRK53_RS07475 0.56 +1.2 1,672,480 + LRK53_RS07475 0.56 -1.1 1,672,480 + LRK53_RS07475 0.56 -0.5 1,672,481 - LRK53_RS07475 0.57 -0.2 1,672,481 - LRK53_RS07475 0.57 +1.2 1,672,481 - LRK53_RS07475 0.57 -1.0 1,672,481 - LRK53_RS07475 0.57 +0.7 1,672,481 - LRK53_RS07475 0.57 +0.5 1,672,570 + -0.5 1,672,570 + +1.2 1,672,570 + -1.1 1,672,570 + +1.2 1,672,570 + +0.2 1,672,570 + -1.1 1,672,570 + +0.2 1,672,571 - -0.5 1,672,571 - +1.2 1,672,571 - -1.3 1,672,571 - -0.3 1,672,639 + +0.5 1,672,639 + -0.3 1,672,639 + -0.2 1,672,639 + +0.8 1,672,639 + -0.2 1,672,640 - -0.1 1,672,640 - -1.1 1,672,640 - -1.8 1,672,640 - -0.8 1,672,640 - -0.9
Or see this region's nucleotide sequence