Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07035 and LRK53_RS07040 are separated by 219 nucleotides LRK53_RS07040 and LRK53_RS07045 overlap by 4 nucleotides LRK53_RS07045 and LRK53_RS07050 are separated by 237 nucleotides
LRK53_RS07035: LRK53_RS07035 - TolC family protein, at 1,582,812 to 1,584,200
_RS07035
LRK53_RS07040: LRK53_RS07040 - sensor histidine kinase, at 1,584,420 to 1,585,835
_RS07040
LRK53_RS07045: LRK53_RS07045 - response regulator, at 1,585,832 to 1,586,521
_RS07045
LRK53_RS07050: LRK53_RS07050 - sulfite reductase flavoprotein subunit alpha, at 1,586,759 to 1,588,441
_RS07050
Position (kb)
1584
1585
1586 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1583.890 kb on + strand, within LRK53_RS07035 at 1583.891 kb on - strand, within LRK53_RS07035 at 1583.891 kb on - strand, within LRK53_RS07035 at 1583.950 kb on + strand, within LRK53_RS07035 at 1583.950 kb on + strand, within LRK53_RS07035 at 1583.951 kb on - strand, within LRK53_RS07035 at 1583.951 kb on - strand, within LRK53_RS07035 at 1583.971 kb on + strand, within LRK53_RS07035 at 1583.971 kb on + strand, within LRK53_RS07035 at 1583.971 kb on + strand, within LRK53_RS07035 at 1583.972 kb on - strand, within LRK53_RS07035 at 1583.972 kb on - strand, within LRK53_RS07035 at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.300 kb on - strand at 1584.339 kb on - strand at 1584.339 kb on - strand at 1585.182 kb on + strand, within LRK53_RS07040 at 1585.182 kb on + strand, within LRK53_RS07040 at 1585.182 kb on + strand, within LRK53_RS07040 at 1585.183 kb on - strand, within LRK53_RS07040 at 1585.183 kb on - strand, within LRK53_RS07040 at 1585.472 kb on - strand, within LRK53_RS07040 at 1585.570 kb on + strand, within LRK53_RS07040 at 1585.570 kb on + strand, within LRK53_RS07040 at 1585.571 kb on - strand, within LRK53_RS07040 at 1585.571 kb on - strand, within LRK53_RS07040 at 1585.591 kb on + strand, within LRK53_RS07040 at 1585.591 kb on + strand, within LRK53_RS07040 at 1585.591 kb on + strand, within LRK53_RS07040 at 1585.591 kb on + strand, within LRK53_RS07040 at 1585.592 kb on - strand, within LRK53_RS07040 at 1585.592 kb on - strand, within LRK53_RS07040 at 1585.708 kb on + strand at 1585.709 kb on - strand at 1585.726 kb on + strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.875 kb on + strand at 1585.899 kb on - strand at 1585.899 kb on - strand at 1586.336 kb on + strand, within LRK53_RS07045 at 1586.336 kb on + strand, within LRK53_RS07045 at 1586.337 kb on - strand, within LRK53_RS07045 at 1586.337 kb on - strand, within LRK53_RS07045 at 1586.337 kb on - strand, within LRK53_RS07045 at 1586.389 kb on - strand, within LRK53_RS07045 at 1586.538 kb on + strand at 1586.538 kb on + strand at 1586.538 kb on + strand at 1586.539 kb on - strand at 1586.539 kb on - strand at 1586.539 kb on - strand at 1586.539 kb on - strand at 1586.609 kb on - strand at 1586.609 kb on - strand at 1586.609 kb on - strand at 1586.609 kb on - strand at 1586.609 kb on - strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.695 kb on + strand at 1586.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,583,890 + LRK53_RS07035 0.78 +2.2 1,583,891 - LRK53_RS07035 0.78 -0.8 1,583,891 - LRK53_RS07035 0.78 +1.2 1,583,950 + LRK53_RS07035 0.82 -0.3 1,583,950 + LRK53_RS07035 0.82 +0.0 1,583,951 - LRK53_RS07035 0.82 -1.3 1,583,951 - LRK53_RS07035 0.82 -0.3 1,583,971 + LRK53_RS07035 0.83 -0.8 1,583,971 + LRK53_RS07035 0.83 -1.9 1,583,971 + LRK53_RS07035 0.83 -0.3 1,583,972 - LRK53_RS07035 0.84 +2.2 1,583,972 - LRK53_RS07035 0.84 +0.2 1,584,270 + +0.7 1,584,270 + +2.2 1,584,270 + +0.7 1,584,270 + +0.2 1,584,270 + +0.2 1,584,270 + +0.2 1,584,270 + -1.1 1,584,270 + +1.2 1,584,270 + +0.2 1,584,270 + -0.1 1,584,270 + +0.7 1,584,270 + +2.2 1,584,270 + -0.8 1,584,270 + -2.8 1,584,270 + +0.2 1,584,270 + -0.8 1,584,270 + -1.1 1,584,270 + +1.2 1,584,270 + -1.1 1,584,270 + +0.2 1,584,270 + -0.3 1,584,270 + +1.2 1,584,271 - -1.8 1,584,271 - -1.2 1,584,271 - +0.4 1,584,271 - -1.1 1,584,271 - -1.9 1,584,271 - +0.9 1,584,271 - +1.8 1,584,271 - +0.2 1,584,271 - +0.2 1,584,271 - +0.2 1,584,271 - -0.8 1,584,271 - -1.1 1,584,271 - +0.2 1,584,271 - -0.8 1,584,271 - -1.3 1,584,271 - -1.2 1,584,271 - +0.7 1,584,271 - -0.1 1,584,271 - +0.2 1,584,271 - -0.8 1,584,271 - -0.8 1,584,271 - -0.3 1,584,271 - -0.1 1,584,271 - +1.2 1,584,271 - -0.8 1,584,271 - -0.3 1,584,271 - +0.5 1,584,271 - +2.2 1,584,271 - -0.5 1,584,271 - -2.1 1,584,300 - +0.2 1,584,339 - -1.6 1,584,339 - +0.2 1,585,182 + LRK53_RS07040 0.54 -2.7 1,585,182 + LRK53_RS07040 0.54 -1.3 1,585,182 + LRK53_RS07040 0.54 +1.2 1,585,183 - LRK53_RS07040 0.54 +1.8 1,585,183 - LRK53_RS07040 0.54 -1.3 1,585,472 - LRK53_RS07040 0.74 +1.2 1,585,570 + LRK53_RS07040 0.81 -1.3 1,585,570 + LRK53_RS07040 0.81 -1.6 1,585,571 - LRK53_RS07040 0.81 -0.9 1,585,571 - LRK53_RS07040 0.81 +0.2 1,585,591 + LRK53_RS07040 0.83 -1.3 1,585,591 + LRK53_RS07040 0.83 +1.2 1,585,591 + LRK53_RS07040 0.83 -0.3 1,585,591 + LRK53_RS07040 0.83 +0.2 1,585,592 - LRK53_RS07040 0.83 +0.7 1,585,592 - LRK53_RS07040 0.83 -0.8 1,585,708 + +1.2 1,585,709 - +0.2 1,585,726 + +0.2 1,585,727 - -0.3 1,585,727 - +1.2 1,585,727 - -1.6 1,585,727 - -3.3 1,585,727 - -1.6 1,585,727 - +1.2 1,585,727 - -1.2 1,585,727 - +0.2 1,585,727 - +0.2 1,585,727 - -1.9 1,585,727 - -0.3 1,585,727 - -0.3 1,585,875 + -2.2 1,585,899 - +1.2 1,585,899 - +1.2 1,586,336 + LRK53_RS07045 0.73 +1.2 1,586,336 + LRK53_RS07045 0.73 -0.6 1,586,337 - LRK53_RS07045 0.73 -0.3 1,586,337 - LRK53_RS07045 0.73 -0.8 1,586,337 - LRK53_RS07045 0.73 +1.2 1,586,389 - LRK53_RS07045 0.81 -0.3 1,586,538 + -2.2 1,586,538 + +0.2 1,586,538 + +0.2 1,586,539 - -1.1 1,586,539 - -0.8 1,586,539 - -1.2 1,586,539 - +0.2 1,586,609 - -0.4 1,586,609 - +0.9 1,586,609 - -0.3 1,586,609 - -1.2 1,586,609 - -0.5 1,586,665 + -0.2 1,586,665 + -0.8 1,586,665 + +1.0 1,586,665 + +0.2 1,586,665 + +0.6 1,586,666 - +1.2 1,586,666 - +0.7 1,586,666 - +1.2 1,586,666 - -1.2 1,586,666 - -1.2 1,586,695 + -1.2 1,586,696 - +0.2
Or see this region's nucleotide sequence