Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06935 and LRK53_RS06940 overlap by 4 nucleotides LRK53_RS06940 and LRK53_RS06945 are separated by 320 nucleotides LRK53_RS06945 and LRK53_RS06950 overlap by 1 nucleotides LRK53_RS06950 and LRK53_RS06955 are separated by 91 nucleotides
LRK53_RS06935: LRK53_RS06935 - type II toxin-antitoxin system VapB family antitoxin, at 1,565,928 to 1,566,143
_RS06935
LRK53_RS06940: LRK53_RS06940 - PIN domain nuclease, at 1,566,140 to 1,566,532
_RS06940
LRK53_RS06945: LRK53_RS06945 - beta-ketoacyl-[acyl-carrier-protein] synthase family protein, at 1,566,853 to 1,568,085
_RS06945
LRK53_RS06950: LRK53_RS06950 - phosphopantetheine-binding protein, at 1,568,085 to 1,568,357
_RS06950
LRK53_RS06955: LRK53_RS06955 - GNAT family N-acetyltransferase, at 1,568,449 to 1,569,519
_RS06955
Position (kb)
1566
1567
1568
1569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.856 kb on - strand at 1565.927 kb on + strand at 1565.928 kb on - strand at 1565.928 kb on - strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.960 kb on + strand, within LRK53_RS06935 at 1565.960 kb on + strand, within LRK53_RS06935 at 1566.173 kb on + strand at 1566.173 kb on + strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.544 kb on + strand at 1566.544 kb on + strand at 1566.544 kb on + strand at 1566.545 kb on - strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.684 kb on + strand at 1566.684 kb on + strand at 1566.685 kb on - strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1567.196 kb on + strand, within LRK53_RS06945 at 1567.196 kb on + strand, within LRK53_RS06945 at 1567.196 kb on + strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.482 kb on - strand, within LRK53_RS06945 at 1567.482 kb on - strand, within LRK53_RS06945 at 1567.482 kb on - strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.619 kb on + strand, within LRK53_RS06945 at 1567.915 kb on + strand, within LRK53_RS06945 at 1567.915 kb on + strand, within LRK53_RS06945 at 1568.430 kb on + strand at 1568.430 kb on + strand at 1568.431 kb on - strand at 1568.431 kb on - strand at 1568.431 kb on - strand at 1568.444 kb on + strand at 1568.444 kb on + strand at 1568.444 kb on + strand at 1568.444 kb on + strand at 1568.445 kb on - strand at 1568.445 kb on - strand at 1568.445 kb on - strand at 1568.445 kb on - strand at 1568.626 kb on + strand, within LRK53_RS06955 at 1568.690 kb on - strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,565,855 + -3.0 1,565,855 + -1.8 1,565,855 + -0.6 1,565,855 + +1.2 1,565,855 + +0.2 1,565,855 + +0.2 1,565,856 - -0.3 1,565,927 + -0.9 1,565,928 - -1.1 1,565,928 - +0.2 1,565,944 + +0.2 1,565,944 + -1.1 1,565,944 + +0.7 1,565,944 + +1.2 1,565,960 + LRK53_RS06935 0.15 +1.2 1,565,960 + LRK53_RS06935 0.15 +1.2 1,566,173 + +0.2 1,566,173 + -0.3 1,566,174 - -1.1 1,566,174 - +0.9 1,566,174 - +0.7 1,566,174 - +0.0 1,566,174 - +0.7 1,566,544 + +1.2 1,566,544 + +0.2 1,566,544 + -2.5 1,566,545 - +1.2 1,566,550 + +0.6 1,566,550 + -0.8 1,566,550 + -0.3 1,566,550 + +0.4 1,566,550 + -0.6 1,566,551 - -0.8 1,566,551 - -0.3 1,566,551 - -0.6 1,566,551 - -0.5 1,566,551 - -0.1 1,566,551 - -0.8 1,566,551 - -0.8 1,566,551 - +0.2 1,566,684 + -0.8 1,566,684 + +0.7 1,566,685 - -0.3 1,566,748 + +1.2 1,566,748 + +1.0 1,566,748 + -0.6 1,566,748 + -0.4 1,566,748 + +0.7 1,566,748 + -1.2 1,566,748 + +0.0 1,566,748 + -0.8 1,566,748 + +0.0 1,566,748 + -0.1 1,566,748 + -0.5 1,566,748 + -0.3 1,566,748 + -0.1 1,566,749 - +1.2 1,566,749 - -1.8 1,566,749 - -1.1 1,566,749 - -0.3 1,566,749 - +1.2 1,566,749 - -0.8 1,566,749 - -0.1 1,566,749 - -0.4 1,566,749 - -0.2 1,566,749 - +0.2 1,566,749 - -0.8 1,566,760 + -0.5 1,566,760 + -1.2 1,566,760 + -1.1 1,566,760 + -0.6 1,566,760 + -1.2 1,566,760 + -0.1 1,566,760 + -0.9 1,566,760 + -2.1 1,566,760 + -1.3 1,566,760 + -0.3 1,566,760 + +0.1 1,566,760 + +1.0 1,566,761 - +1.2 1,566,761 - +1.2 1,566,761 - +0.2 1,566,761 - -2.2 1,566,761 - +0.0 1,566,761 - -0.8 1,566,761 - +0.7 1,566,761 - +0.1 1,566,761 - -1.3 1,566,956 + +1.2 1,566,956 + -0.3 1,566,956 + -0.3 1,566,956 + -2.0 1,566,956 + +1.2 1,566,956 + -0.3 1,566,956 + +1.2 1,566,957 - +1.2 1,566,957 - -0.3 1,566,957 - -0.6 1,566,957 - -1.3 1,566,957 - +1.2 1,566,957 - -1.6 1,566,957 - +1.2 1,567,196 + LRK53_RS06945 0.28 +0.2 1,567,196 + LRK53_RS06945 0.28 -2.9 1,567,196 + LRK53_RS06945 0.28 +2.2 1,567,197 - LRK53_RS06945 0.28 -2.1 1,567,197 - LRK53_RS06945 0.28 -1.3 1,567,197 - LRK53_RS06945 0.28 -0.1 1,567,197 - LRK53_RS06945 0.28 +0.2 1,567,197 - LRK53_RS06945 0.28 -1.6 1,567,197 - LRK53_RS06945 0.28 +0.2 1,567,197 - LRK53_RS06945 0.28 -1.9 1,567,197 - LRK53_RS06945 0.28 -0.8 1,567,197 - LRK53_RS06945 0.28 +1.2 1,567,482 - LRK53_RS06945 0.51 +0.8 1,567,482 - LRK53_RS06945 0.51 -1.1 1,567,482 - LRK53_RS06945 0.51 +0.2 1,567,598 + LRK53_RS06945 0.60 +0.2 1,567,598 + LRK53_RS06945 0.60 -1.3 1,567,598 + LRK53_RS06945 0.60 -0.6 1,567,599 - LRK53_RS06945 0.61 -0.8 1,567,599 - LRK53_RS06945 0.61 -0.8 1,567,599 - LRK53_RS06945 0.61 +0.2 1,567,599 - LRK53_RS06945 0.61 +1.2 1,567,599 - LRK53_RS06945 0.61 -0.8 1,567,599 - LRK53_RS06945 0.61 -1.1 1,567,599 - LRK53_RS06945 0.61 -2.2 1,567,599 - LRK53_RS06945 0.61 -1.3 1,567,599 - LRK53_RS06945 0.61 -1.9 1,567,599 - LRK53_RS06945 0.61 +0.2 1,567,599 - LRK53_RS06945 0.61 -0.2 1,567,619 + LRK53_RS06945 0.62 +0.2 1,567,915 + LRK53_RS06945 0.86 -3.2 1,567,915 + LRK53_RS06945 0.86 +0.2 1,568,430 + +0.1 1,568,430 + +0.2 1,568,431 - +0.7 1,568,431 - -1.6 1,568,431 - +0.2 1,568,444 + -0.3 1,568,444 + -0.8 1,568,444 + -1.8 1,568,444 + -0.3 1,568,445 - -1.2 1,568,445 - -2.6 1,568,445 - -1.3 1,568,445 - -0.9 1,568,626 + LRK53_RS06955 0.17 -1.1 1,568,690 - LRK53_RS06955 0.23 -0.1 1,568,929 + LRK53_RS06955 0.45 -1.5 1,568,929 + LRK53_RS06955 0.45 -2.3 1,568,929 + LRK53_RS06955 0.45 +1.0 1,568,929 + LRK53_RS06955 0.45 -0.6 1,568,929 + LRK53_RS06955 0.45 -1.5 1,568,929 + LRK53_RS06955 0.45 +0.8 1,568,929 + LRK53_RS06955 0.45 +1.2 1,568,929 + LRK53_RS06955 0.45 -1.9 1,568,929 + LRK53_RS06955 0.45 +1.0 1,568,929 + LRK53_RS06955 0.45 -2.2 1,568,930 - LRK53_RS06955 0.45 +0.2 1,568,930 - LRK53_RS06955 0.45 -0.1 1,568,930 - LRK53_RS06955 0.45 +0.2 1,568,930 - LRK53_RS06955 0.45 -0.3 1,568,930 - LRK53_RS06955 0.45 +0.7 1,568,930 - LRK53_RS06955 0.45 -1.5 1,569,082 + LRK53_RS06955 0.59 +0.2 1,569,082 + LRK53_RS06955 0.59 -0.8 1,569,082 + LRK53_RS06955 0.59 -0.2 1,569,082 + LRK53_RS06955 0.59 -1.6 1,569,082 + LRK53_RS06955 0.59 -0.8 1,569,082 + LRK53_RS06955 0.59 -1.3 1,569,082 + LRK53_RS06955 0.59 +1.2 1,569,082 + LRK53_RS06955 0.59 -0.8 1,569,083 - LRK53_RS06955 0.59 -0.8 1,569,083 - LRK53_RS06955 0.59 -0.6 1,569,083 - LRK53_RS06955 0.59 +1.2 1,569,083 - LRK53_RS06955 0.59 -0.1 1,569,083 - LRK53_RS06955 0.59 +0.2 1,569,083 - LRK53_RS06955 0.59 -2.5 1,569,083 - LRK53_RS06955 0.59 +0.7 1,569,083 - LRK53_RS06955 0.59 +0.7
Or see this region's nucleotide sequence