Experiment: R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06920 and LRK53_RS06925 are separated by 140 nucleotides LRK53_RS06925 and LRK53_RS06930 are separated by 53 nucleotides LRK53_RS06930 and LRK53_RS06935 are separated by 564 nucleotides LRK53_RS06935 and LRK53_RS06940 overlap by 4 nucleotides
LRK53_RS06920: LRK53_RS06920 - MipA/OmpV family protein, at 1,562,279 to 1,563,064
_RS06920
LRK53_RS06925: LRK53_RS06925 - MtnX-like HAD-IB family phosphatase, at 1,563,205 to 1,563,906
_RS06925
LRK53_RS06930: LRK53_RS06930 - aminotransferase class III-fold pyridoxal phosphate-dependent enzyme, at 1,563,960 to 1,565,363
_RS06930
LRK53_RS06935: LRK53_RS06935 - type II toxin-antitoxin system VapB family antitoxin, at 1,565,928 to 1,566,143
_RS06935
LRK53_RS06940: LRK53_RS06940 - PIN domain nuclease, at 1,566,140 to 1,566,532
_RS06940
Position (kb)
1563
1564
1565
1566 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1563.077 kb on + strand at 1563.077 kb on + strand at 1563.077 kb on + strand at 1563.077 kb on + strand at 1563.078 kb on - strand at 1563.078 kb on - strand at 1563.078 kb on - strand at 1563.535 kb on + strand, within LRK53_RS06925 at 1563.799 kb on + strand, within LRK53_RS06925 at 1563.799 kb on + strand, within LRK53_RS06925 at 1563.799 kb on + strand, within LRK53_RS06925 at 1563.799 kb on + strand, within LRK53_RS06925 at 1563.800 kb on - strand, within LRK53_RS06925 at 1564.029 kb on + strand at 1564.029 kb on + strand at 1564.030 kb on - strand at 1564.056 kb on + strand at 1564.056 kb on + strand at 1564.056 kb on + strand at 1564.057 kb on - strand at 1564.057 kb on - strand at 1564.060 kb on + strand at 1564.098 kb on + strand at 1564.098 kb on + strand at 1564.098 kb on + strand at 1564.099 kb on - strand at 1564.146 kb on + strand, within LRK53_RS06930 at 1564.146 kb on + strand, within LRK53_RS06930 at 1564.374 kb on + strand, within LRK53_RS06930 at 1564.374 kb on + strand, within LRK53_RS06930 at 1564.458 kb on + strand, within LRK53_RS06930 at 1564.558 kb on - strand, within LRK53_RS06930 at 1564.605 kb on + strand, within LRK53_RS06930 at 1564.644 kb on + strand, within LRK53_RS06930 at 1564.656 kb on + strand, within LRK53_RS06930 at 1564.686 kb on + strand, within LRK53_RS06930 at 1564.740 kb on + strand, within LRK53_RS06930 at 1565.474 kb on + strand at 1565.474 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.856 kb on - strand at 1565.927 kb on + strand at 1565.928 kb on - strand at 1565.928 kb on - strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.960 kb on + strand, within LRK53_RS06935 at 1565.960 kb on + strand, within LRK53_RS06935 at 1566.173 kb on + strand at 1566.173 kb on + strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride hemipentahydrate 128 uM remove 1,563,077 + +1.2 1,563,077 + -0.6 1,563,077 + +0.2 1,563,077 + +1.8 1,563,078 - -0.9 1,563,078 - +0.0 1,563,078 - +0.2 1,563,535 + LRK53_RS06925 0.47 -0.3 1,563,799 + LRK53_RS06925 0.85 -1.1 1,563,799 + LRK53_RS06925 0.85 +1.2 1,563,799 + LRK53_RS06925 0.85 -0.1 1,563,799 + LRK53_RS06925 0.85 -1.1 1,563,800 - LRK53_RS06925 0.85 +0.2 1,564,029 + -1.1 1,564,029 + +1.7 1,564,030 - -2.6 1,564,056 + +1.2 1,564,056 + -0.8 1,564,056 + -0.3 1,564,057 - -1.1 1,564,057 - -2.5 1,564,060 + -0.3 1,564,098 + +0.2 1,564,098 + -0.8 1,564,098 + +0.7 1,564,099 - -0.8 1,564,146 + LRK53_RS06930 0.13 +0.2 1,564,146 + LRK53_RS06930 0.13 +1.2 1,564,374 + LRK53_RS06930 0.29 +0.2 1,564,374 + LRK53_RS06930 0.29 +1.2 1,564,458 + LRK53_RS06930 0.35 -1.6 1,564,558 - LRK53_RS06930 0.43 +0.2 1,564,605 + LRK53_RS06930 0.46 -0.3 1,564,644 + LRK53_RS06930 0.49 +1.2 1,564,656 + LRK53_RS06930 0.50 +0.2 1,564,686 + LRK53_RS06930 0.52 +0.2 1,564,740 + LRK53_RS06930 0.56 -0.3 1,565,474 + +0.4 1,565,474 + -1.0 1,565,855 + -3.0 1,565,855 + -1.8 1,565,855 + -0.6 1,565,855 + +1.2 1,565,855 + +0.2 1,565,855 + +0.2 1,565,856 - -0.3 1,565,927 + -0.9 1,565,928 - -1.1 1,565,928 - +0.2 1,565,944 + +0.2 1,565,944 + -1.1 1,565,944 + +0.7 1,565,944 + +1.2 1,565,960 + LRK53_RS06935 0.15 +1.2 1,565,960 + LRK53_RS06935 0.15 +1.2 1,566,173 + +0.2 1,566,173 + -0.3 1,566,174 - -1.1 1,566,174 - +0.9 1,566,174 - +0.7 1,566,174 - +0.0 1,566,174 - +0.7
Or see this region's nucleotide sequence